the cartesian coordinate system is formed by the
Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
Ngày tải lên :
18/02/2014, 13:20
... proteins
within the cell. We found this translocation to be
dependent on the final amino acids of the receptor
protein. Similar to the other PDZ domain interactions
that have been shown to be abolished by ... signal is impaired.
We finally examined the effect of deletion of the
components of the PDZ binding motif on the signal-
ing properties of the receptor in Ca
2+
imaging
experiments. Truncation of the ... summary, when the interaction between
MUPP1 and OR2AG1 is inhibited by over-expressing
the interacting PDZ domains, the resulting response of
OR2AG1 to odorant is modified in that the rapid
decay...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... Fenton
reaction with the iron metal at the active site. The presence
of iron is therefore a prerequisite for its effect and suggests
that the absence of reactivity with the MnSOD mutants is
due to the lack ... identified to distinguish between FeSOD and
MnSOD [7]. The position of the glutamine residue, which is
structurally equivalent in the enzymes, is contributed by the
N domain of FeSODs (Q69) and the C ... a shift in
the position of Y34 and a twist in the ring of the metal-
binding H81. Thermostability is not a new phenomenon
amongst SODs; those isolated from hyperthermophilic
organisms, for example,...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
Ngày tải lên :
07/03/2014, 05:20
... Subsequently, the
GST–PP2Aca:His–TIPRL complex was incubated with
His–a4 and the GST–PP2Aca:His–a4 complex was
incubated with His–TIPRL. Binding of His–TIPRL to
the previously formed GST–PP2Aca:His–a4 ... order to test this hypothesis, in vitro assays
were performed in which the activity of PP2A core
enzyme (A and C subunits) was measured in the pres-
ence of His–a4 or His–TIPRL using the phosphopep-
tide ... PP2Ac is
represented by a black bar for comparison. Numbers on the left of the gray bars indicate the first amino acid in the respective activation
domain-phosphatase catalytic subunit fusion. The...
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Báo cáo khoa học: Androgen receptor function is modulated by the tissue-specific AR45 variant ppt
Ngày tải lên :
07/03/2014, 16:20
... recruitment, due to the
absence of the NTD. This might be overcome by the
overexpression of cofactors. To test this hypothesis, we
analyzed the effects of two AR cofactors known to
interact with the AR LBD. ... observed. The important role of the NTD, which
is absent in AR45, has been documented by several
studies [40–42]. The hormone-dependent interaction
between this region and the LBD of the AR is essen-
tial ... primer specific for the extreme 5¢ region was
then used together with a primer recognizing the 3¢ end
of the AR coding part to amplify the complete coding
region of this novel AR form. The deduced amino...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
Ngày tải lên :
07/03/2014, 21:20
... mutagenesis of the
N-terminus of the V
1a
R; this revealed that a single residue
(Arg46) located in the distal segment of this domain is
critical for high affinity agonist binding but not antag-
onist ... affinity agonist binding, by site-
directed mutagenesis. The wildtype Arg46 was systematic-
ally substituted by all the other 19 encoded amino acids.
These receptor constructs were then characterized ... rescued
by a chimeric construct in which the N-terminus of the
OTR is replaced with the corresponding sequence of
the V
1a
R [10]. The role of the N-terminus has been
addressed recently by alanine-scanning...
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Báo cáo khoa học: The bioactive dipeptide anserine is transported by human proton-coupled peptide transporters doc
Ngày tải lên :
15/03/2014, 09:20
... see
[11,12]). At the renal epithelium, small peptides are
reabsorbed from the glomerular filtrate into the cells
by the subtypes PEPT1 and PEPT2. PEPT2 is also
expressed in other tissues, such as ... insertion of the correct
cDNA was verified by sequencing. The pNKS-hPEPT1
construct served as template for cRNA synthesis. After
linearizing the plasmids with NotI, cRNAs were synthesized
using the mMESSAGE ... of vertebrates [1,2]. It is formed
as a secondary product through the methylation of the
dipeptide carnosine (b-alanyl-l-histidine) [3]. The reac-
tion is catalysed by carnosine N-methyltransferase
[3,4]....
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Báo cáo khoa học: Proteolytic degradation of nitric oxide synthase isoforms by calpain is modulated by the expression levels of HSP90 potx
Ngày tải lên :
23/03/2014, 07:20
... and
obviously the isolated proteins are degraded by the
protease. On the basis of these observations, we can
explain not only the protective effect exerted by
HSP90 on the digestion of NOS isozymes, ... can
prevent the degradation by calpain of both NOS by
protecting the cleavage of the peptide bond close to
the CaM binding site. This explains why both NOS
are inactivated by calpain in the absence ... quantified as described in the Exper-
imental procedures. The areas of the peaks were normalized on
the basis of the amount of protein loaded on the column. The val-
ues reported are the arithmetical mean...
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Metaphor, based on the association of similarity, is one of the two basic types of semantic transference that have been an interest for many linguistic researchers
Ngày tải lên :
07/11/2012, 14:44
... is thus a theory of the global distribution of the units of the grammar.
The English grammatical rank scale recognized by Systemic Functional Grammar is as follows:
In turn, each unit has their ... English Theme is the first element of a clause and providing what we call the departure for
the starting point for the clause. By changing Theme we do not change the ideational meaning
but the ... what is it that the participants are engaged in, in which the language is an
essential component?
+ The TENOR OF DISCOURSE concerns who is taking part, to the nature of the participants,
their...
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![Tài liệu [ Team LiB ] Configuring Your Safe Senders List The Safe Senders list is part of the Junk E-mail pdf](https://media.store123doc.com/images/document/14/nu/js/medium_jsx1390245650.jpg)
Tài liệu [ Team LiB ] Configuring Your Safe Senders List The Safe Senders list is part of the Junk E-mail pdf
Ngày tải lên :
21/01/2014, 02:20
... Safe Senders List
The Safe Senders list is part of the Junk E-mail filters. If you choose to allow external
content from addresses listed in your Safe Senders list, you need to check the
configuration ... select Add
Sender to Safe Senders List or Add the Domain @[domain-name] to Safe Senders List.
The sender's fully qualified domain name is listed in the menu selection, as shown
previously ... you need to check the
configuration of the list.
You can add addresses and domains to the Safe Senders list directly from an email
message. Click on the InfoBar or right-click in any image...
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Tài liệu The Role of BCG Vaccine in the Prevention and Control of Tuberculosis in the United States: A Joint Statement by the Advisory Council for the Elimination of Tuberculosis and the Advisory Committee on Immunization Practices docx
Ngày tải lên :
15/02/2014, 13:20
... as the disease became less prevalent; however, the risk is
still high for HCWs who work in settings in which the incidence of TB among patients
is high. The precise risk for TB among HCWs in the ... developed at the University of
Illinois (Chicago, Illinois) from a strain originated at the Pasteur Institute. The Food and
Drug Administration is considering another vaccine, which is produced by Connaught
Laboratories, ... with the
Advisory Council for the Elimination of Tuberculosis
and the
Advisory Committee on Immunization Practices
vi MMWR April 26, 1996
Advisory Council for the Elimination of Tuberculosis (ACET)
September...
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Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx
Ngày tải lên :
08/03/2014, 02:20
... tyrosines, by the
PTK Lck, to be deduced.
Experimental procedures
Peptide synthesis
Peptides were made by manual solid-phase peptide synthesis
using the tBoc method [11], purified by RP-HPLC, ... sample. The analysis was repeated for fragment 5–6, the
other high-yielding product of the (incomplete) trypsin
digest in which the only ITAM Tyr was 1N. This procedure
was performed for all of the ... 2, and the farthest as 3. The two tyrosines
within each ITAM are then further classified as N or C,
reflecting their locations relative to the N-terminus and
C-terminus (Fig. 1). Six synthetic...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
Ngày tải lên :
17/03/2014, 17:20
... level of GR in the epithelia of the
epididymis (Fig. 1 ). As the epididymis is rich in sulfoxidase,
the oxidation of sulfhydryls in the sperm head and tail
would be mediated by this enzyme in a ... Thiol oxidation occurs
in the nuclei and the tail during the maturation process in
the epididymis. The greater the extent of oxidation in nuclei,
the more potent are the spermatozoa. In addition, ... prostate gland. The l ocalization of
GR, as evidenced by immunohistochemical techniques,
reveals that it exists at high levels in the epithelia of the
genital tract. In testis, GR is mainly localized...
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