... of these reports provide evidence of
post-fertilisation transferand maintenance of transgenes. Several ofthe studies report the subsequent
generation of viable progeny animals, the cells of ... germline gene therapy reconsidered. Hum Gene Ther. 2001; 12(11): 144 9-
145 8.
3. McDonough PG. The ethics of somatic and germline gene therapy. Adolescent Gynecology and
Endocrinology- Basic and Clinical ... KR. The role of sperm-mediated gene transfer in genome mutation and evolution. Med Hypotheses
2002; 59 (4) :43 3 -43 7.
39. Zani M, Lavitrano M, French D, Lulli V. The mechanism of binding of exogenous...
... of attack of 60°
shows better
heat transfer performance compared to that ofthe 45 ° rib arrangement. Lau et al. [6, 7] investigated the
heat transferand friction factor characteristics of ...
Figure 4. Laser sheet arrangement for PIV measurement of single rib
4. Results and discussion
The results of experimental investigation ofheattransferand flow characteristics ofthe roughened ... value of turbulent intensity is observed at the relative gap width of 1.0 which is in the line ofheat
transfer measurements. These results of PIV showed good agreement between theheat transfer...
... thecondensationand fragmentation of their
nuclei. The percentage of apoptotic cells was calculated as
the number of apoptotic cells divided by the total number
of cells counted. A minimum of ... Institutional
Animal Care and Use Committee ofthe Center South Uni-
versity, and were carried out in accordance with the
National Institutes of Health Guide for the Care and Use
of Laboratory Animals. ... Journal 277 (2010) 642 652 ê 2009 The Authors Journal compilation ê 2009 FEBS 651
After 24 h of H
2
O
2
exposure, 44 % of untransfected
control cells and 45 % of cells transfected with the
empty vector...
... convection. In the first section of this system, heat is transferred
by convection andradiation in parallel. Hence, the rate ofheat flow to the interior
surface ofthe wall is the sum ofthe two heat ... ft
2
. The water is at 200°F andthe surrounding
air is at 80°F. If theheattransfer coefficients between the
water andthe glass, and between the glass andthe air are
5 Btu/h ft
2
°F and 1.2 ... Basic Modes ofHeatTransfer 2
1.1 The Relation ofHeatTransfer to Thermodynamics 3
1.2 Dimensions and Units 7
1.3 HeatConduction 9
1 .4 Convection 17
1.5 Radiation 21
1.6 Combined Heat Transfer...
... the solid andthe liquid.
Instead, the structure of solid and liquid co-exists within a
certain range between the solid andthe liquid, which is shown
as the co-existence ofthe peak andthe ... atoms, andthe system consists of 48 6,000 atoms. In
both x and y directions, the computational domain has a size of
48 .73 nm. In the z direction, the size ofthe computation domain
is 17. 14 nm ... to the
surface ofthe target by scaling velocities of atoms in cells on
the top surface, andthe same amount ofheat flux is dissipated
from the bottom ofthe target by scaling velocities of...
... the solid andthe liquid.
Instead, the structure of solid and liquid co-exists within a
certain range between the solid andthe liquid, which is shown
as the co-existence ofthe peak andthe ... atoms, andthe system consists of 48 6,000 atoms. In
both x and y directions, the computational domain has a size of
48 .73 nm. In the z direction, the size ofthe computation domain
is 17. 14 nm ... check the validity ofthe simulation, thermal
physical properties including the specific heat at constant
pressure, the specific heat at constant volume, andthe thermal
conductivity are calculated...
... the solid andthe liquid.
Instead, the structure of solid and liquid co-exists within a
certain range between the solid andthe liquid, which is shown
as the co-existence ofthe peak andthe ... to the
surface ofthe target by scaling velocities of atoms in cells on
the top surface, andthe same amount ofheat flux is dissipated
from the bottom ofthe target by scaling velocities of ... constant 0. 541 4 nm
c
r , Cut off distance
0.8515 nm
Size ofthe sample –
x 48 .726 nm
Size ofthe sample –y 48 .726 nm
Size ofthe sample –z 8.121 nm
Time step 25 fs
Number of atoms 48 6000
...
... open,
transparent, and readily apparent alignment between the goals of learning
programs andthe needs ofthe business. We share suggestions and case
studies on how learning and line leaders can work together ... asked to
estimate the annual value created as a result ofthe program. We tally
these and we show the results of what they have done as a group and
for the program as a whole. They are self-reported ... ofthe senior man-
agers we say: ‘These are your people and this is what they are saying.’
And once the talent management council participates in a call
and really hears the stories behind the...
... Comparison of amino acid sequences of SH2 domains from Src andthe mouse Grb4, and expression and purification ofthe Grb4 SH2 and
RGS3 PDZ domains. (A) Secondary structural elements are from the X-ray ... question by use of the
SH2 domain of Grb4 andthe PDZ domain ofthe RGS3
protein. We titrated the SH2 protein into the fluorescein-
labeled ephrinB2(301–333)-pY3 04 after saturation by the
GST-PDZ ... residues Lys306 and Tyr316, Glu305 and
Fig. 6. Effect of PDZ binding on the interactions ofthe Grb4-SH2
domain with the ephrinB2(301–333)-pY3 04 peptide. (A) The PDZ-
binding curves are for the fluorescein-labeled...
... loops
L
2Â3Â
(Thr412-Tyr417) and L
4 5Â
(Gln4 34- Arg 441 ).
These two loops interact with the tips of four other
loops: L
11Â
(Gly378-Asp375), L
2Â2
(Ala402-Arg4 04) ,
L
3 4
(Gly4 24- Gly427) and L
66Â
(Glu498-Gly500). ... in the axis
area areof polar character. The water channel in the
apo form ofthe enzyme runs along the axis of the
b-propeller through the whole domain and is only
blocked by Cys236 in the area ... ID: 3KF5
2.3 ⁄ 44 2 ⁄ 24 2.1 ⁄ 40 8 ⁄ 25 2.0 ⁄ 46 8 ⁄
35 – 2.6 ⁄ 44 8 ⁄ 23 2.7 ⁄ 45 2 ⁄ 24
Invertase Arabidopsis thaliana PDB ID: 2AC1 2.0 ⁄ 45 6 ⁄ 28 2.1 ⁄ 41 6 ⁄ 26 2.5 ⁄ 46 1 ⁄ 28 2.6 ⁄ 44 9 ⁄ 23 – 1.2...
... cold-inducible. Hsp40 is an essential cofactor
that interacts with the members ofthe Hsp70 family.
It accelerates the dissociation ofthe ADP–Hsp70 com-
plex, and therefore an increase in Hsp40 concentration
may ... using the absolute quantifi-
cation module in the software package. The PCR reactions
were performed in a final volume of 10 lL containing 1 lL
of cDNA sample, 0 .4 lm each primer, and 5 lL ofthe ... pupariation and emergence. Cryobiology 33,
44 7 45 8.
44 Sun Y & MacRae TH (2005) Small heat shock proteins:
molecular structure and chaperone function. Cell Mol
Life Sci 62, 246 0– 247 6.
45 Kim M...
... For the calibration ofthe oxygen electrode,
the oxygen content ofthe air-saturated incubation medium
was taken to be 217 nmolặmL
)1
[49 ].
Immunoblotting of cytochrome c
A volume of 2 mL ofthe ... 35933601 ê 2005 FEBS
The increases in cytosolic Ca
2+
and ROS concentra-
tion are two essential factors that favour opening of
the mPTP [45 ]. Thereby oxidation of SH-groups of the
adenine nucleotide ... to the Bradford method
[47 ] using BSA as the standard. In separate experiments we
determined protein values with the Bradford and Lowry
methods. From the comparison, a correction factor of 1 .4
was...
... changes in
the population ofthe fish shoal, based on the
success or failure of its reproduction, the
surviving rate ofthe fish generation within its
life cycle andthe migration of additional ... means of fishing. These arethe
differences from the long-term forecast. The
changes of oceanological factors in the forecast
area such as temperature, salinity, currents,
disturbance and displacement ... June and July, 2009 presented up to 60 percentage of acception.
Meanwhile, the quantity of good forecasts are about 50% andthe quantity of excellent forecasts
ranks from 25 to 41 %.
The Length...
... that
babies are born with 90 percent of their brains formed and millions of connections that
are surplus. So the rst years of life are spent winnowing out the unused connections
and growing the ones ... parts of their brains interact to form a variety of overlapping sensory
associations. In other words, the rats were not just seeing their way to the food in the
maze; they were smelling and feeling ... sample of baby boomers “When does old age begin?” The
average answer was 85. As expectations rise, clearly the brain must keep pace and
accommodate the new old age. The old theory ofthe xed and...