suppression and alteration of adhesion structures in human epithelial cancer progression

Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps

Ngày tải lên : 12/08/2014, 13:22

... Servant, R Zollinger, SI Bogiatzi, P Hupé, E Barillot, V Soumelis, A critical function for transforming growth factor-β, interleukin 23 and proinflammatory cytokines in driving and modulating human TH-17 ... Generation and Differentiation of Th17 Cells and Tregs in MPE Purified naïve CD4+ T cells (5 × 105) were cultured in ml of complete medium containing human IL-2 (2 ng/ ml) in 48-well plates and stimulated ... Cells in MPE We determined some cytokines that reported be involved in generation and differentiation of Tregs or Th17 cells, and observed that high levels of IL-1b, IL-6, and TGF-b1, but not of...

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Experimentation, modeling and control of calcium dynamics in human vascular endothelial cells

Ngày tải lên : 09/09/2015, 18:48

... identifying the structure of the mechano-sensor in VECs membrane, ﬁnding signaling pathway given certain type of mechanical stimulus and investigating the interplay of gene expression and cell ... biological/physiological and engineering perspectives Proﬁcient skills in handling cells and chip In our case, we need to integrate cell culture, ATP release measurement and Ca2+ imaging into the one system The main ... concentration of ATP on cell surface, in uencing calcium mobilization in an indirect fashion In the meanwhile, there were several other groups providing their results supporting Ando’s point of view...

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Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt

Ngày tải lên : 28/03/2014, 14:20

... as mRNA and protein Further, mRNAs and proteins of MMP-2, -9, -15 and -23 were moderately expressed in this cell line According to this finding we also detected gelatinolytic activity of secreted ... Expression of matrix metalloproteinase-26 and tissue inhibitors of metalloproteinase-3 and -4 in normal ovary and ovarian carcinoma Int J Gynecol Cancer 2006, 16(5):1794-800 Page 11 of 12 18 Giambernardi ... we investigated the expression of all MMPs known in humans so far by measuring mRNA and protein level in twelve gynecological cancer cell lines commonly used in experimental research We examined...

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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Ngày tải lên : 21/02/2014, 01:21

... cystatin F, through analysis of the expression, distribution and properties of the native human inhibitor in U937 cells MATERIALS AND METHODS In silico cloning and sequencing of the human cystatin ... in the ER staining where cystatin F stains Regulation of cystatin F expression To shed light on the regulation of the cystatin F gene, we examined the sequence of the human gene, CST7 At an initial ... characterization and use of monoclonal antibodies against the major extracellular human cysteine proteinase inhibitors cystatin C and kininogen Scand J Clin Laboratory Invest 48, 573–582 Bjarnadottir,...

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Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

Ngày tải lên : 07/03/2014, 14:20

... observed in studies utilizing a series of human colorectal carcinoma cell lines [7] and tissue obtained from colorectal cancer patients [3,7] The level of mono-Oacetylated Neu5Ac (Neu5,9Ac2 and Neu5,7Ac2) ... for the alteration of O-acetylated sialic acids in cancer mucosa, the activities of OAE-L, OAE-C and OAT in the resection margins and cancer mucosa from matched tissue samples were determined As ... mono-Oacetylation remaining constant [7,30] In this study, utilizing matched colonic samples (resection margin and cancer tissue obtained from the same colorectal carcinoma patients) at all stages of cancer...

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Environmental Injustice and Human Rights Abuse: The States, MNCs, and Repression of Minority Groups in the World System pptx

Ngày tải lên : 15/03/2014, 23:20

... justice and the need for international codification of norms pertaining to the rights of all people to clean air, water, and a safe and sound environment capable of sustaining life are offered in ... number of cases involving ecological and human rights problems ranging from the military government extermination of indigenous population in Irian Jaya, Indonesia, to ecological assaults and human ... are inseparable from several cases of environmental injustice and human rights repression including genocide in the Third World Unfortunately, most of the practices involving environmental and human...

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Báo cáo khoa học: Identiﬁcation and characterization of multiple species of tenascin-X in human serum doc

Ngày tải lên : 23/03/2014, 09:21

... previously mapped binding of these proteins to the FNIII29 of TNX in a study of C-terminal TNX domains [20] The recombinant TNX domains in our previous study were produced in prokaryotic cells ... and Physiology of the Washington University School of Medicine are acknowledged for performing N-terminal sequencing and providing recombinant tropoelastin Claire Lethias of the Institut de Biologie ... significant binding to human collagen type I, III and V; binding to human fibronectin, laminin or irrelevant protein (BSA) was also absent (data not shown) Similarly, we did not find any effect of serum...

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Báo cáo khoa học: Characterization of sequence variations in human histone H1.2 and H1.4 subtypes pptx

Ngày tải lên : 30/03/2014, 20:20

... tail and the binding of H1.4 to chromatin, as arginine offers additional hydrogen-bonding abilities to DNA as compared to lysine Lysine 173 in H1.4 is situated in an SPKK motif, one of the known ... believed to be involved in positioning of the globular domain on the nucleosome [32] The N- and C-terminal tails of histone H1 adopt a random coil in solution [33] On binding of histone H1° to DNA, ... the binding of H1.2 to chromatin, may be affected A further nonsynonymous substitution prompted the replacement of lysine with arginine in the C-terminal tail (position 173) of histone H1.4 in...

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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps

Ngày tải lên : 09/08/2014, 07:21

... cut-offs for positivity and negativity We determined the expression of E-cadherin, α-, β- and γcatenin in human breast carcinomas using real time quantitative polymerase chain reaction and investigated ... prognosis and non-invasive properties[21] Junctional catenin expression is often lost in cadherin-negative breast cancer and changes in catenin phosphorylation may compromise adhesion in cadherinpositive ... Journal of Surgical Oncology 2008, 6:56 The main structure of E-cadherin catenin complex consists of transmembrane E-cadherin, cytoplasmic proteins, called catenins (α-, β- and γ-catenin), and actin...

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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf

Ngày tải lên : 09/08/2014, 09:20

... were obtained from ATCC and maintained in a humidified atmosphere of 5% CO2 and 95% air 10 mg of DCQ was dissolved in ml of DMSO and stored in a brown eppendorf at 4°C and then diluted in media ... [8] In a recent study, DCQ was found to induce single strand breaks (SSB) in DNA of DLD-1 human colon cancer cells, and both SSB and double strand breaks (DSB) in EMT6 cells [5,11] DNA damage, in ... compound that induces apoptosis in several murine and human cancer cell lines [4,5,8] This is our first attempt to understand the role of p53 and p21 in drug efficacy using colon cancer cells...

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báo cáo khoa học: "Dysregulation of miR-15a and miR-214 in human pancreatic cancer" potx

Ngày tải lên : 10/08/2014, 22:21

... cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Gibco BRL) 293T cells were maintained in DMEM containing 10% FBS Ten samples of pancreatic cancer tissues and their ... Xiaohua S, Xinyu R, Kai W, Tonghua L: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines Mol Cancer ... roles in the development of the disease Overexpression of exogenous miR-15a inhibited the viability of pancreatic cancer cells, suggesting that downregulation of miR-15a might be involved in the progression...

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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx

Ngày tải lên : 11/08/2014, 00:22

... participated in the conception and experimental design of the in vitro HIV-1 manipulation and infection assays, in the analysis and interpretation of the data, and in the writing and revision of this ... working designing, and authoring E.N.G-T participated in the analysis and interpretation of the data and in writing and revising this report C.R-P participated in the experimental design of this study ... PVP-coated AgNPs After 48 h of incubation in a 37°C humidified incubator, the tissues were washed, embedded in paraffin, and stained with hematoxylin and eosin b) Cervical explants in the upper Transwell...

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báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx

Ngày tải lên : 11/08/2014, 00:22

... Mondalek et al., Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP Journal of Nanobiotechnology ... Angiogenesis in Gastric Cancer Cancer Microenviron 2009 in press 14 Waugh DJ, Wilson C: The interleukin-8 pathway in cancer Clin Cancer Res 2008, 14(21):6735-41 15 Nishimoto N: Interleukin-6 in rheumatoid ... the design planning of all experiments PP participated in the design of XTT, CAM and Western experiments, assisted in data analysis and interpretation and in writing and revising the manuscript...

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Báo cáo khoa học: "Changes in some Blood Micronutrients, Leukocytes and Neutrophil Expression of Adhesion Molecules in Periparturient Dairy Cows" ppt

Ngày tải lên : 12/08/2014, 15:20

... before and after calving in the blood levels of Ca, P, K and Na (Dukes 1993) Blood levels of Ca and P is expected to decrease at calving due to the large demand of colostrum and milk production In ... collected in the morning, using Vacutainer® tubes (Becton Dickinson Vacutainer Systems, Meylan, France), one month before estimated calving, at calving (within 24 hours after calving) and one month ... at calving in serum concentrations of vitamins A and E, and in Zn in agreement with earlier reports (Johnston & Chew 1984, Goff & Stabel 1990, Weiss et al 1990, Xin et al 1993) A drop in the...

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Báo cáo y học: " Epratuzumab targeting of CD22 affects adhesion molecule expression and migration of B-cells in systemic lupus erythematosus" potx

Ngày tải lên : 12/08/2014, 15:21

... tyrosine kinase) which is involved in BCR signaling, but also in the regulation of integrin activation [44] Moreover, recent work indicates that Syk is involved in CXCL12- and a4b1 integrin-induced ... expression of a4b7 integrin is regulated in a homeostatic relation with the surface expression of a4b1 integrin on T-cells since high expression of a4b1 integrin resulted in a loss of a4b7 integrin With ... effects of epratuzumab on the expression of a range of adhesion molecules (CD62L, b1 integrin and b7 integrin) and migration towards CXCL12, CXCL13 and a number of CXCR3 ligands (CXCL9, 10 and 11)...

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Báo cáo y học: " Caspase-3-mediated cleavage of p65/RelA results in a carboxy-terminal fragment that inhibits IκBα and enhances HIV-1 replication in human T lymphocytes" ppsx

Ngày tải lên : 13/08/2014, 05:21

... 1,0 Figure Binding affinity assay of p65wt-tag and ΔNH2p65-tag to IκBα by using in vitro translated proteins Binding affinity assay of p65wt-tag and ΔNH2p65-tag to IκBα by using in vitro translated ... along with p65wt-tag In fact, overexpression of increasing quantities of ΔNH2p65-tag protein in the 3T3-p65ko cell line – maintaining the p65wttag protein concentration as invariable – showed ... Figure DNA-binding and transcriptional activity of ΔNH2p65 Analysis of Analysis of DNA-binding and transcriptional activity of ΔNH2p65 (a) Proteins p65wt-tag, p65 D94E;D97E-tag, ΔNH2p65-tag and NF-κB1/p50...

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Báo cáo y học: "A novel model of common Toll-like receptor 4- and injury-induced transcriptional themes in human leukocytes" pot

Ngày tải lên : 13/08/2014, 21:21

... resulted in the identification of 445 genes (5.4%) that persisted in differential expression in response to TLR4induced systemic inflammation and/ or injury We refer to this group of TLR4 and injury ... 29 Marsin AS, Bouzin C, Bertrand L, Hue L: The stimulation of glycolysis by hypoxia in activated monocytes is mediated by AMP-activated protein kinase and inducible 6-phosphofructo-2-kinase J ... differentially expressed genes during the early, dynamic phase of TLR4-induced inflammation resulting from endotoxin infusion, and those differentially expressed in PBL in the early posttrauma time...

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Báo cáo y học: "Large-scale discovery and validation of functional elements in the human genome" ppt

Ngày tải lên : 14/08/2014, 14:21

... will offer an additional way of obtaining a global understanding of regulatory elements John Stamatoyannopoulos (Regulome, Seattle, USA) and Gregory Crawford (National Human Genome Research Institute ... identification of regions of open chromatin in the human genome The methods involve mapping DNase I hypersensitive sites, which are known to correlate with many types of functional elements, including promoters, ... state -of- the-art technologies being applied to the identification of functional elements in genomes are producing huge numbers of candidate regions High-throughput assays in human cells and model...

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Báo cáo y học: "Systematic analysis of gene expression in human brains before and after death" docx

Ngày tải lên : 14/08/2014, 16:20

... expression patterns in postmortem human brains Study of expression levels of 14 genes in human brain autopsy and biopsy samples found significant change in one of the genes, indicating that a substantial ... organization and biogenesis' and 'intracellular protein transport' (Table 2) The expression of genes involved in the ubiquitin cycle and protein ubiquitination is significantly increased after ... translated to functional proteins, up-regulation of genes involved in ubiquitin-dependent protein catabolism may lead to increased degradation of proteins in human brain samples after death This...

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Actual situation study, some factors has risk to infect larva of toxocara canis in human and effect of treatment by albendazole at 2 commune of an nhon district, binh dinh province (2011 2012)

Ngày tải lên : 29/10/2014, 22:17

... roundworm larvae in human; in dogs, in surroundings (soil) 2.2.6 Use definitions and concepts in research: The infection ratio of dog roundworm larvae in human; in dogs, in surroundings (soil); ... prior to interviewing, investigating and interviewing cadres must be trained and a trial interview will be made in the locality 2.2.8 Parameter handling: collected parameters will be handled by ... (36.0%) and having habit of eating fresh vegetable (26.0%) [30] According to P.A.M Overgaauw and et at (1997), no increase of morbidity rate of larvae of Toxocara canis in those working in veterinary...

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