structure functions and classification of amino acids
Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
Ngày tải lên :
16/03/2014, 06:20
... substrates, and probably allows the interaction of the enzyme with the substrate structure Whether the motion of the lid promotes the recognition of the substrate structure, or the recognition of this structure ... PLRP2 and colipase ) no interaction colipase, and what the nature is of the amino acids that correspond to the exposed surface The superposition of hoPL and hoPLRP2 (Fig 6) shows that loop b5¢ of ... PLRP2 and colipase ) no interaction In the absence of dithiothreitol in the sample buffer, native hoPL and hoPLRP2 ran as a single band of about 50 kDa (Fig 2A, lanes and 2) In the presence of dithiothreitol...
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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot
Ngày tải lên :
30/03/2014, 10:20
... acetylcholine-binding proteins and the b ECD have similar structures and that the secondary structures of a non-a ECD (b) and the a ECD resemble one another, being largely composed of b -structure Thirdly, ... supernatant using dot-blots and a series of supernatant dilutions Expression varied depending on the presence of the different modifications (Fig 1B) The yield of amino acids 1–218 of the human AChR c ... right site and possibly of correct structure Secondly, the CD spectrum of the b ECD indicated a folded protein consisting mainly of b-sheet (Fig 4) The solved crystallographic structures of the molluscan...
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Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot
Ngày tải lên :
18/03/2014, 01:20
... indication of ligand-independent preassociation of the receptor chains Therefore, the dimerization of LIFR and gp130 can be used as a measure of high affinity ligand binding Deletion of individual ... coprecipitation of LIFR and gp130c (lanes and 2), coexpression of LIFR with gp130C458Ac led to the ligand-independent coprecipitation of both receptor chains independently of stimulation (lanes and 4, ... transfection of COS7 cells with LIFR and the gp130 chimeras coprecipitation and detection of LIFR and gp130 was performed as described in (A) LIFR and gp130, a prominent signal of coprecipitated...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
Ngày tải lên :
15/03/2014, 09:20
... site By contrast, the active center of nitrogenase-2 consists of vanadium and iron, and that of nitrogenase-3 contains only iron [1,2] The expression of each set of structural genes is regulated ... primary structure of the GAF domain is highly conserved in VnfA and AnfA, whereas NifA shares little homology with them, suggesting that the sensor structure of NifA is distinct from that of VnfA and ... combination of the presence and absence of AMPPNP and the Fe-S cluster As described above, N-terminal cysteine residues located immediately upstream of the GAF domain are the potential ligands of the...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
Ngày tải lên :
15/03/2014, 11:20
... superimposition of ligand (substrate or inhibitor)-free and ligand-bound structures In (F), only the ligand-free structure is shown because no ligand-bound structure is available The side-chains of three ... 2.88 2.72 3.18 2.84 Od2 of Asp423 OH of Ser425 NH of Ala490 NE1 of Trp664 O3 O6 O7 O7 2.89 2.64 2.94 2.40 NH of Ala490 Od2 of Asp636 Od1 of Asp524a Od2 of Asp524a O7 2.63 OH of Tyr590 NAG3 ()1) NAG4 ... Ala524 Asp522 Fig Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B) The structures of bound sugars and the side-chains of three acidic residues in...
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Báo cáo khoa học: Dissecting the role of the N-terminal metal-binding domains in activating the yeast copper ATPase in vivo pptx
Ngày tải lên :
23/03/2014, 05:22
... Ccc2 and the mammalian copper ATPases all possess a cytosolic N-terminus made of between two and six metal-binding domains (MBD) and each of these domains is similar to Atx1 in terms of structure, ... complementation assays to assess the transport of copper inside the TGN by Ccc2 [8] and human homologues [23,24], or to investigate the role of various amino acids in these proteins [25–28] Although ... Expression of Ccc2 using the pX vector Immunodetection of endogenous Ccc2 in the wild-type strain YPH499 (1) and immunodetection of Ccc2 provided by the pX– vector in the presence of Atx1 (pC–Atx1)...
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt
Ngày tải lên :
23/03/2014, 06:20
... mutants (A) SDS ⁄ PAGE of purified full-length ClpC1 and deletion mutants, ClpC1D1 and ClpC1D2 (B) Full-length ClpC1 and deletion mutants, ClpC1D1 and ClpC1D2; the first and last amino acid numbers ... in its pathogenesis and an understanding of 6150 their mode of action could be useful in exploring them as drug targets Results Figure shows the sequence and putative domains of M tuberculosis ... ClpC1D1 and ClpC1D2 were generated in which, respectively, amino acids 1–38 and 1–77 were deleted from the N-terminus of M tuberculosis ClpC1 (Fig 2B) ClpC1D1 has the N-terminal repeat I deleted, and...
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Báo cáo hóa học: " Contribution of cysteine residues in the extracellular domain of the F protein of human respiratory syncytial virus to its function" docx
Ngày tải lên :
20/06/2014, 01:20
... processing of F0 to F1 and F2 Mutation of residues 69 and 322 dramatically reduced the levels of total protein immunoprecipitated as well the levels of F0 processed to F1 and F2 None of the mutations ... mutagenesis of the HRSV F protein JL generated the computer model of the HRSV F ECD AD and RS participated in the design of the experiments, oversight of the conduct of the experiments, and AD, RS, and ... sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit Nucleic Acids Res 1985, 13:1559-1574 Zimmer G, Trotz I, Herrler G: N-glycans of F...
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Báo cáo y học: " Role of the C-terminal domain of the HIV-1 glycoprotein in cell-to-cell viral transmission between T lymphocytes" potx
Ngày tải lên :
12/08/2014, 23:23
... addition of uninfected cells and culture medium Lysates of equal numbers of cells, now containing equal percentages of infected cells, were prepared, and protein determination using standard procedures ... Replicative spread of HIV-Wt and HIV-Env-Tr712 in different T-cells A Schematic depiction of the Env proteins from HIV-Wt and HIVEnv-Tr712 B Replication kinetics of HIV-Wt (circles) and HIV-Env-Tr712 ... levels of Figure HIV gag gene expression in non-permissive (np) and permissive (p) cells A Western blot analysis employing antibodies to HIV-CA and cellular tubulin of equal amounts of lysates of...
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Báo cáo y học: "Role of the long cytoplasmic domain of the SIV Env glycoprotein in early and late stages of infection" ppt
Ngày tải lên :
13/08/2014, 05:22
... [25] The early steps of HIV and SIV infection include the attachment of viruses to host cells, entry and transport of the genome to the transcription site, formation of the PIC, and import to the ... fetal calf serum and antibiotics, and buffered by 10 mM HEPES Preparation of cloned SIV stocks, standardization of virus titers, and conditions for virus infection were done as Page of 14 (page number ... http://www.retrovirology.com/content/4/1/94 Figure of early steps of replication of SIV with length or truncated Env in rhesus monkey PBMC full Comparison Comparison of early steps of replication of SIV with full length...
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The role of the n terminal extension domain of vamp4 in the regulation of its recycling to the TRANS GOLGI network
Ngày tải lên :
11/09/2015, 09:02
... presumably unstructured Habc Habc Habc Habc ND ND D12 ND Yes ND, similar to Slt1 Localization EE and RE TGN and EE PM LE and Lysosome and PM EE and LE Golgi ER and ERGIC ERGIC and cis-Golgi ER and ERGIC ... General structure of SNAREs 25 1.3.2 General mode of action of SNAREs 37 1.3.3 1.4 1.2.2 1.3 Vesicular transport 1.2.1 1.2 SNARE localization and the specificity of transport 41 44 VAMPs and the ... patience, understanding and most important, their love Tran Thi Ton Hoai 2009 ii Table of contents Thesis title Acknowledgement i Table of contents iii Summary viii List of Tables x List of Figures...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
Ngày tải lên :
25/10/2012, 11:15
... three regions between RIZ1 and ephrin receptor (EphR) during sequence analysis (Fig 2) The sequence identity and homology were 34.2% and 68.4%, 37.0% and 81.5%, and 22.9% and 53.1% in the three aligned ... alone exhibited anticancer activity Materials and Methods Overexpression and purification of PR domain The cloning, overexpression and purification of recombinant His6-tagget PR domain has been ... respectively Western blot of PR domain The expression of the PR domain was examined after 24 hr of transfection with pcDNA/HisMax/PR and the empty vector, respectively Supernatants (30 g) of the cell lysates...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
Ngày tải lên :
14/02/2014, 19:20
... in a small subset of individuals, mirroring a highly balanced degree of co-evolution of H pylori and humans Studies of host–bacterial interactions and virulence factors CagA and the T4SS have ... mechanism of H pylori-associated carcinogenesis A first direct causal link between CagA and oncogenesis Fig Models for the assembly and assembled structure of T4SS in A tumefaciens and Helicobacter ... kDa, domain structure and amino acid positions shown for CagY of strain 26695, accession number NP_207323.1) carrying two transmembrane domains (TM1 and TM2) to form a hairpin-loop structure in...
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Tài liệu Rethinking the Role of the State in Finance doc
Ngày tải lên :
16/02/2014, 10:20
... Nippon Life Professor of Finance and Professor of Economics at the Wharton School of the University of Pennsylvania Thorsten Beck Professor of Economics and Chairman of the European Banking Center, ... Netherlands Charles Calomiris Henry Kaufmann Professor of Financial Institutions, Graduate School of Business, Columbia University Gerard Caprio William Brough Professor of Economics and Chair, ... various official interventions in financial systems, from regulation and supervision of financial institutions and markets, to competition policy, to state guarantees and state ownership of banks, and...
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Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt
Ngày tải lên :
16/02/2014, 15:20
... is a member of the TALE superfamily of HD proteins, which are characterized by the presence of a three amino acid loop insertion between helices and of the HD [13–15] The presence of this loop ... 5¢-end of exon (Fig 7A) The region missing in Meis3.2 encodes the equivalent of amino acids 164–180 in Meis2, which form about half of hr2 (see Fig 6A) To confirm that the two isoforms of Meis3 ... presence of alternative splicing around the 5¢-end of exon of Meis2 and Meis3 was tested by RT-PCR The positions of molecular mass markers are shown to the left, and the size in base pairs of the...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
Ngày tải lên :
19/02/2014, 00:20
... crystal structure of the complex of this protease with benzamidine Results and Discussion Overall structure The catalytic domain of DESC1 resembles an oblate ˚ ellipsoid with diameters of 38 and ... mediate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and proper positioning of the scissile ... and emerging subfamily of serine proteases Crystal structure of the catalytic domain of DESC1 Experimental procedures Cloning, expression and purification Cloning, expression and purification of...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
Ngày tải lên :
19/02/2014, 07:20
... respectively, p-quinones and dimeric quinonic structures (Fig 1) [7] Tyrosinases are responsible for vertebrate cutaneous pigmentation, browning of fruits and vegetables, and morphogenesis and fruiting body ... to be part of the defense system against wounding and infection by the pathogen [16] Thus, the timing and location of melanization seems to be essential for the prevalence of one of these two ... of the histidine residues of the CuA and CuB binding regions and the same Prosite signatures [2,4,6] The low or zero monophenolase ⁄ o-diphenolase ratio is understandable Chemical oxidation of...
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