structural and functional roles of fsh and lh as glycoproteins regulating reproduction in mammal

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

Ngày tải lên : 20/02/2014, 01:20

... [17] The structural TnC-binding site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity of the troponin complex ... strengthening of the interaction between the structural TnCbinding site and the C-domain of TnC accompanying Ca2+ binding to site IV of TnC In vertebrate troponin, the activation may be a result of the interaction ... ATPase activity of the thin ﬁlament J Biol Chem 272, 10529–10537 29 Ramos CH (1999) Mapping subdomains in the C-terminal region of troponin I involved in its binding to troponin C and to thin...

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báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf

Ngày tải lên : 11/08/2014, 11:22

... cotransformed into host yeast strain NMY51 Interaction was determined by the growth of yeast transformants on medium lacking His or Ade and also by measuring bgalactosidase activity Functional complementation ... heteromeric interactions of COPTs AIg5, a transmembrane protein with C terminal in the cytoplasm; NubI, N-terminal half of ubiquitin protein; NubG, mutated N-terminal half of ubiquitin protein; Cub, ... root and COPT3 and COPT7 in shoot, and slightly suppressed COPT2 and COPT4 in root Zn deficiency induced COPT1, COPT5, and COPT7 and slightly suppressed COPT4 in root and induced COPT5, COPT6, and...

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Báo cáo y học: "Rapid and persistent selection of the K103N mutation as a majority quasispecies in a HIV1-patient exposed to efavirenz for three weeks: a case report and review of the literature" pot

Ngày tải lên : 11/08/2014, 14:21

... persistence of K103N mutation after EFV interruption in patients failing to EFV-containing regimens, documenting variable decay kinetics for the K103N containing quasispecies Such quasispecies ... minimal exposures to EFV, reinforcing at the same time the need for a systematic evaluation of GRT in naïve patients and after any type of interruption of NNRTI-based HAART regimens, as well as ... transcriptase inhibitor; NVP, nevirapine; PI, protease inhibitor Competing interests The authors declare that they have no competing interests Consent Written informed consent was obtained from...

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Understanding the functional roles of intrinsic protein disorder in NFkB transcription factors

Ngày tải lên : 12/10/2015, 17:35

... roles of intrinsic protein disorder in cytoplasmic signaling domains[22] and in scaffold proteins, which integrate cell signaling pathways[15], have been reported The most relevant study of intrinsic ... conservation and functional roles of intrinsically disordered protein residues in NFκB signaling machinery For my research project, an in silico approach was adopted since large-scale data mining and ... involved in many biological events and pathways, especially during cell signaling[14,15,22-24] 1.3.1 Role of Intrinsic Protein Disorder in Cell Signaling In the context of cell signaling, intrinsically...

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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

Ngày tải lên : 20/02/2014, 11:20

... mobility binding assays with increasing concentrations of control Ydc2 and Ydc2-ND35 (A) or Ydc2-ND35/CD15 (B), as indicated, visualized by phosphorimaging (C) Binding reactions with increasing concentrations ... mutants of Ydc2–GFP were introduced in strain JAL01 Vector pREP41–EGFP in JAL01 was used as control Expression of the fusion proteins was induced by growth in the absence of thiamine and was veriﬁed ... and mutations in mtDNA associated with mitochondrial diseases and implicated in aging (reviewed in [39]) Understanding the molecular mechanisms and pathways of mtDNA inheritance in ﬁssion yeast,...

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LONDON AND PARIS AS INTERNATIONAL FINANCIAL CENTRES IN THE TWENTIETH CENTURY pot

Ngày tải lên : 29/06/2014, 00:20

... Preeminence of International Financial Centers (New York, 1981); G Jones, ‘International Financial Centres in Asia, the Middle East and Australia’, in Y Cassis (ed.), Finance and Financiers in ... wars has indicated a willingness to provide both short- and long-term funds as long as there was a prospect of the business surviving and prospering Only when that was not the case was finance ... and markets that was required to organize, evaluate, and finance major projects in such fields as transport and mining, considering the risks and uncertainties involved.7 Lying at the heart of...

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Báo cáo khoa học: " Correlation between HIV viral load and aminotransferases as liver damage markers in HIV infected naive patients: a concordance cross-sectional study" pdf

Ngày tải lên : 12/08/2014, 04:20

... aminotransferases with HCV RNA levels and histological findings in patients with chronic hepatitis C: the role of serum aspartate transaminase in the evaluation of disease progression Eur J Gastroenterol ... theories of liver damage due to mitochondrial disturbance and the stimulation of the caspases cascade in the induction of apoptosis including those without antiretroviral experience Based in our ... proteins which stimulate an inflammatory response [9-16] Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are hepatic enzymes that could be used as markers of hepatocellular injury...

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Báo cáo y học: "Functional consequences of DECTIN-1 early stop codon polymorphism Y238X in rheumatoid arthritis" pptx

Ngày tải lên : 12/08/2014, 11:23

... Dectin-1 mRNA and protein expression in synovial tissue To gain insight into the distribution and amount of dectin-1 expression in human synovial tissue, dectin-1 mRNA expression was measured in ... 0.98 DAS28: Disease Activity Score using 28 joint counts Baseline and follow-up values of disease markers of joint inflammation and bone destruction of 262 RA patients, stratified by DECTIN-1 ... implicated in immunopathology Furthermore, dectin-1 is involved in the induction of arthritis in mouse models through induction of intracellular signalling on recognition of fungal components As a...

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Báo cáo y học: " pG island density and its correlations with genomic features in mammalian genomes" pps

Ngày tải lên : 14/08/2014, 08:21

... monotremes and has a fascinating mixture of features typical of mammals and of reptiles and birds Monotremes (mammalian subclass Prototheria) are the oldest branch of the mammalian tree, diverging 210 ... sites, leading to TpG and CpA dinucleotides Excess of TpGs and CpAs as well as other vanishing CGI features (decreasing length, ObsCpG/ExpCpG ratio and GC content) has been found in the homologous ... a faster rate of loss of CGIs in the rodent lineage, rather than faster gains of CGIs in the human lineage [7,9] However, it remains unclear whether the loss -of- CGI model holds for other mammalian...

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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc

Ngày tải lên : 23/03/2014, 10:20

... against HNE–protein adducts Ligandbinding tests showing the functionality of the same HNE-treated porcine (B) and bovine (C) OBPs as in the immunostaining The plots show the residual AMA-binding ... incubations, in which at least 50% of residual ligand-binding capacity was maintained at the highest [HNE] ⁄ [OBP] values In the case of 20-min incubations, HNE inactivation was even less efﬁcient and ... even in the case of very high incubating [HNE] ⁄ [OBP] molar ratios (50 : 1), both forms lost only a fraction of their initial AMA-binding capability Western blotting and ligand-binding assay of...

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The effect of CDK1 mediated GOLGI vesiculation on mitotic progression in mammalian cells

Ngày tải lên : 16/10/2015, 15:35

... studied in yeasts and mammalian cells It consists of two phases, the interphase and the mitotic phase (Mphase) The interphase in turn is made up of three phases; the first gap phase (G1-phase), ... consists of 986 amino acids and has coiled coil domains It has an N terminal region that binds to p115 Its C terminal was shown to interact with another Golgi structural protein GRASP65 Giantin, on ... might also be involved in the G2 Golgi fragmentation Table 1.1 List of kinases involved in regulating Golgi structure and function Table modified from: (Lowe and Barr 2007) Kinases Interaction...

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Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

Ngày tải lên : 07/03/2014, 12:20

... centrifugation Monomer mass refers to the mass of p26 monomers and was calculated using a p26 molecular mass of 20.8 kDa, as determined by GENERUNNER (version 3.05, Hastings Software, Inc.) with corrections ... Modeling indicates that tetramer formation depends upon contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands and in the a-crystallin domain of monomers C and ... against physiological stress, was investigated by site-directed mutagenesis of a-crystallin domain residues and molecular modeling of protein structure The p26 a-crystallin domain contains nine...

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Báo cáo y học: "Summary The claudin multigene family encodes tetraspan membrane proteins that are crucial structural and functional components of tight junctions, which have important roles in regulating para cellular" pdf

Ngày tải lên : 14/08/2014, 21:20

... Claudin-17 Claudin-5 ‘Classic’ claudins ‘Non-classic’ claudins Claudin-10b Claudin-15 Claudin-9 Claudin-11 Claudin-6 Claudin-4 Claudin-18a Claudin-3 Claudin-18b Claudin-20 Claudin-12 Claudin-14 ... Claudin-14 Claudin-2 Claudin-19b Claudin-19a Claudin-16 Claudin-7 Claudin-1 Claudin-21 Claudin-24 Claudin-22 Claudin-23 Figure A phylogenetic tree of full-length human claudin proteins, indicating the ... highlighted in green As previously suggested [7], claudins can be divided in two groups in terms of sequence homology (dashed line): the ‘classic’ human claudins are indicated in red and the ‘non-classic’...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... functions as a catalytic base [12] However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transition state stabilization during ... Mutations of Asp13 7in human HPRT to Asn resulted in a 290-fold and 18-fold decrease in kcat values for nucleotide formation with guanine and hypoxanthine bases, respectively, indicating that Asp137 ... being added to crystallization solutions The ligand bound was interpreted as GMP because the N2 of the base makes a hydrogen bond to a main chain carbonyl Binding of a xanthine base from XMP in...

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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

Ngày tải lên : 18/02/2014, 12:20

... proteins work as a DNA-binding protein It is possible that they act as an essential structural component of a multiprotein complex and ⁄ or are directly or indirectly involved in mediating the assembly ... of the cysteines was present in a thiol form in the oxidized protein (except for one in WhiB6 which has five cysteines), it was concluded that the apo form of all WhiB proteins contained two intramolecular ... protein disulfide reductase activity of WhiB2, WhiB5, WhiB6 and WhiB7 by insulin disulfide reduction assay This is a standard assay to asses the disulfide reductase activity of any protein in which...

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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... life of cytokine mRNAs For this reason, transcription of genes encoding cytokines is not sufﬁcient to guarantee the appearance of functional proteins As reported by Kaspar and Gehrke [18] and Dinarello ... amino acids located within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and ... double-stranded RNA-binding domain (dsRBD) and Piwi Argonaut and Zwille (PAZ), represents an RNA-binding domain These domains are usually linked to larger globular enzymatic domains, such as nucleases...

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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

Ngày tải lên : 18/02/2014, 16:20

... tight main-chain and medium side-chain restraints Model building was carried out in o [29] and coot [30] In chain A, residues and 169–176 are missing; in chain B, residues 167–172 are missing; in ... in chain C, residues 1, and 169–174are missing; in chain D, residues 168–174 are missing; in chain F, residues and 168–175 are missing All amino acid residues were present in the E chain, and part ... NADH and lmolÆ min)1Æmg)1ÆmL)1 of pyruvate kinase and lactate dehydrogenase Nucleoside diphosphate kinase (NDPK) was not added, as this did not lead to a signiﬁcant change in the rates determined,...

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Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

Ngày tải lên : 19/02/2014, 07:20

... heart and limb in vivo as efficiently as VEGF and PlGF-1 [77] PDGF-C mediates increased mRNA and protein levels of metalloproteinase-1 (MMP-1) and its inhibitor (TIMP-1), both being important in ... treatment increased both mRNA and protein levels of the metalloproteinase inhibitor, TIMP-1, in dermal fibroblasts in vitro [78] The PDGF-C induction of fibrosis starts with activation and intense ... fibrosis inducer as the PDGF-C overexpression resulted in significantly increased mRNA and protein levels of key profibrotic proteins like PDGFR-a and -b, TGFb1, and TIMP-1 and -2 in the liver of transgenic...

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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

Ngày tải lên : 06/03/2014, 00:21

... the ﬁrst and last turns of helices E and F, and the C-terminus helices I and J In addition, there is a lysine-rich N- and C-terminus, in accordance with the basic isoelectric point of CagS However, ... of pro -in ammatory production of cytokines, mainly interleukin (IL)-8 [12] The activation of a pro -in ammatory signalling cascade has been conﬁrmed to be Nod1 cytoplasmic receptor dependent and ... apparatus In this minireview, we concentrate on this last class of cagPAI proteins, and summarize what is known about them both from a molecular and structural point of view, as well as their...

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