structural and functional organization of the insect retina

structural and functional organization of the synapse

Ngày tải lên : 06/07/2014, 15:30

... STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE Edited by Johannes W Hell University of Iowa Iowa City, IA, USA and Michael ... deciphering the structural and functional organization of the synapse We have assembled leaders in the field of synapse biology to describe and distill the wonders and mysteries of the synapse ... must occur within the tiny space of the synapse, the precise molecular organization of proteins, lipids, and membranes at the synapse is paramount Given the central role of the synapse in neuronal...

807
506
0

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... strength [20] In the present study, we report the structural and functional characterization of the human HPRT homolog PRTFDC1 To shed light on the potential function of PRTFDC1, the protein was ... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in...

11
770
0

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Ngày tải lên : 07/03/2014, 16:20

... to the cytosol Here we conﬁrm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites A schematic representation of the Pex1p and ... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The...

12
584
0

Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf

Ngày tải lên : 08/08/2014, 14:20

... subcloned into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5’region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation ... [4,16,18], and none of them correspond to nitrogen metabolism We have studied the promoter activity of the 5’-untranslated region using fusions with the reporter gene uidA and the presence of DNA-protein ... tissues of developing seedlings and the level of the transcript was affected by developmental and light conditions [9] Here, we present the DNA sequence of a partial genomic clone containing exons of...

6
327
0

Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

Ngày tải lên : 06/03/2014, 00:21

... glutamate and aspartate residues conﬁned to the portion of the molecule involving a-helix A, the nearby loop, the ﬁrst and last turns of helices E and F, and the C-terminus helices I and J In ... b-strand, F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction between monomers ... as that of other members of this family Finally, when crystallized in the presence of Cu(II), the protein shows the presence of the ion coordinated in a small cavity of the surface at the polar...

9
496
0

Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

Ngày tải lên : 16/03/2014, 10:20

... energies of the original resiDG i;j due i in the native protein with the rest of the residues, self DDG k mutị are the changes of the self energies of the residues k that change either their ionization ... on the reaction Analysis of the effect of the mutations on protein stability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation at all The rst type of ... increased by one order of magnitude with respect to the wild-type enzyme, at the expense of the Km value The overall effect of this structural change is a twofold increase in the kcat Km ratio with...

11
437
0

Báo cáo khóa học: Structural and functional comparison of 15S- and 15R-speciﬁc cyclooxygenases from the coral Plexaura homomalla potx

Ngày tải lên : 23/03/2014, 12:20

... proteins, and an Ile in the 15RCOX [22,31] To determine the role of residue 349 in the speciﬁcities of the two P homomalla variants, a Val349Ile mutant of the 15S-COX and an Ile349Val mutant of the ... Swissprot on the basis of three-dimensional data of mammalian COX isozymes (data not shown), reveals that the other 16 residues lie outside of the active site and mostly on the surface of the catalytic ... form the disulphide bonds The residues that are different between the novel COX and the 15R-COX are dispersed along the polypeptide chain with most of them located in the C-terminal half of the...

6
414
0

Báo cáo y học: "Summary The claudin multigene family encodes tetraspan membrane proteins that are crucial structural and functional components of tight junctions, which have important roles in regulating para cellular" pdf

Ngày tải lên : 14/08/2014, 21:20

... representation of the claudin monomer The model depicts the conserved structural features of claudins and some of the known interactions and modifications EL1 and EL2 denote the extracellular loops and ... research in the field of claudins and TJs From a practical point of view, a better under tanding of TJ s formation and regulation may provide novel avenues for the enhancement of drug delivery and absorption ... be extremely variable in terms of number and complexity depending on the cell type Claudins are the major constituents of these strands and from various lines of evidence it has been suggested...

7
348
0

Structural and functional characterization of haditoxin, a novel neurotoxin isolated from the venom of ophiohagus hannah (king cobra

Ngày tải lên : 10/09/2015, 08:37

... fields of study Clearly, rather than the intended offensive and defensive purposes of the toxins, they go a long way for the benefit of science and medicine This becomes a dramatic turning point of ... α-bungarotoxin from the venom of the banded krait (Bungarus multicinctus) (Chang and Lee, 1963), which pioneered the identification and isolation of the nicotinic acetylcholine receptor, the first and most ... (Selected as “Paper of the Week” and for the cover page illustration for the March 12, 2010 issue of the JBC) (4) Roy A, Rajagopalan N, and Kini RM Identification and characterization of a α-helical...

308
442
0

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... independently of the presence of AREs (Fig 6B, lanes and 5) It is possible that in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the IL-1b transcript, there is a putative ... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... many stimulants, and regulates the level of IL-1b mRNA and of its own transcript Further studies are necessary to clarify the biological role of MCPIP as a nuclease and to identify the cis-acting...

14
598
0

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

Ngày tải lên : 18/02/2014, 16:20

... to the side chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and ... in E coli The probable binding motif for the uracil base is through hydrogen bonds from N3 to the backbone O of Phe133, and from O4 to the backbone N and the side chain of Thr131, with the ribose ... hydrogen-bonded to the three lysines (Fig 3E) There is no water molecule ﬁxing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and...

12
656
0

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Ngày tải lên : 18/02/2014, 18:20

... a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure ... assess the structural stability of the Rep245 mutant Following 30 incubation at 60, 70 and 80 °C, we recorded the CD spectra of the incubated Rep245 samples at these temperatures The absence of thermal ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then...

14
620
0

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Ngày tải lên : 21/02/2014, 15:20

... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain ... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... not the lipase activity and that the normal afﬁnity of these mutants for heparin (Fig 3, Table 1) implies conservation of the overall structure of the WW and WWW mutants – indicate that these...

10
679
0

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

Ngày tải lên : 16/03/2014, 06:20

... mostly by serines or glycines The coordination of Ca2+ by the )Z glutamate is the prime reason for the movement of the outgoing helix and the conformational change of EF-hand proteins upon Ca2+ binding ... concluded that EF-hand of the Iba proteins is not capable of functional Ca2+ binding Function of EF-hand In both Iba1 crystal structures, the loops of EF-hand adopt essentially the same conformation ... EF-hand proteins The second pair of EF-hands in calmodulin (PDB code 1CLL) [19] and the second pair of EF-hands in troponin C (PDB code 1NCX) [20] are structurally closely related to Iba2 The...

14
546
0

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

Ngày tải lên : 16/03/2014, 18:20

... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid ... of the medium and K2 the orientation factor, determined by the mutual spatial orientation of the transition dipole moments of the donor and acceptor As no data on the spatial orientation of the ... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary...

10
501
0

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

Ngày tải lên : 16/03/2014, 23:20

... signiﬁcantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... in Fig 4A–D, the rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The eﬀect of b1B and b1 subunits on the function and expression ... encoded by the part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary of the SCN1B...

9
415
0

Báo cáo Y học: Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72 pptx

Ngày tải lên : 17/03/2014, 17:20

... by the ratio of the integral intensities of the signals in the major and minor series, the average degree of glycosylation with GlcII is 75% Comparison of the 13C NMR spectra of the initial and ... con®guration of the Gal residue [19] Signi®cant low-®eld displacements of the signals for C6 of Glc, C3 and C4 of Gal, and C3 of GalNAcI to d 66.8, 81.3, 77.1, and 81.6 in the 13C NMR spectrum of the ... and GalNAcII H1/Gal H3 at d 4.61/3.96 These data ®tted well with the substitution pattern of the sugar residues determined by the 13C NMR chemical shift data and de®ned the full sequence of the...

6
560
0

Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx

Ngày tải lên : 23/03/2014, 21:20

... of the original a2M disappeared and a Analysis of the specific cleavage of a2M by cathepsin E with various synthetic peptides To further confirm the specific cleavage of a2M by cathepsin E, synthetic ... by cathepsin D On the other hand, the pentapeptides SPAFL and SSAFL were not cleaved either cathepsin E or cathepsin D, indicating that the presence of Ala in the P¢2 site is crucial for the selective ... cleavage of a2M by cathepsin E and that the addition of Phe to the P¢3 site causes a loss of this selective action of cathepsin E In agreement with these results, the Fig Effects of synthetic peptides...

10
315
0

Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

Ngày tải lên : 24/03/2014, 03:21

... glycosylation site in the N-terminal extension sequence [13] Therefore, the goal of this study was to investigate the structural and functional characteristics of smelt type II AFP The effects and inter-relationship ... intensity of a higher-molecular-mass band The apparent molecular mass of this band was 38 kDa, which is substantially higher than the molecular mass of the monomer band, indicating the presence of a ... deglycosylated and untreated smelt AFP (both 130 lM) as described in Materials and methods The top row of photos is of ice crystals with their c axis in the plane of the page The bottom row shows...

8
518
0

Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx

Ngày tải lên : 30/03/2014, 15:20

... guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 of B as well as an ionic interaction between E21/Q282 of D3 and R65/S322 of B stabilize the dimer further However, the latter ... ﬂexibility of the C-terminal tail of ataxin-3 [72] Furthermore, the ANTH domain itself is evolutionarily, structurally, and functionally related to a VHS domain [101] Lately, the structure of the GAT ... asparagines N20 and N21, and another identical glutamine Q24 downstream of the catalytic cysteine C14 cannot be derived either from the structural model of the Josephin domain (Figs and 7) However,...

16
526
0