structural and functional modulation of ion channels by specific lipids from model systems to cell membranes

Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx

Ngày tải lên : 23/03/2014, 21:20

... presentation by class II major histocompatibility complex [24] and possible regulation of activities of substance P and related tachykinins [31], whereas cathepsin D is indispensable for protection of ... the presence of Ala in the P¢2 site is crucial for the selective cleavage of a2M by cathepsin E and that the addition of Phe to the P¢3 site causes a loss of this selective action of cathepsin ... Institute of Tropical Medicine, Nagasaki University) for helpful discussion and technical advice and Drs Tsutomu Iwamoto (1st Department of Oral and Maxillofacial Surgery, Nagasaki University School of...

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Structural and functional characterization of haditoxin, a novel neurotoxin isolated from the venom of ophiohagus hannah (king cobra

Ngày tải lên : 10/09/2015, 08:37

... Interaction of haditoxin with AChBP 180 x 5.3.2 Interaction of haditoxin with 5-HT3 receptors 180 5.3.3 Recombinant expression and purification of haditoxin 182 5.3.4 Functional characterization of ... purification of haditoxin 59 2.3.2 Identification of haditoxin 62 2.3.3 Assessment of homogeneity of haditoxin 62 2.3.4 Secondary structure analysis of haditoxin 65 2.4 DISCUSSION 66 2.5 CONCLUSIONS ... subtypes of cholinergic receptors In this study, we report the purification, structural and functional characterization of a novel non-covalent homodimeric neurotoxin, haditoxin, from the venom of...

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Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus

Ngày tải lên : 14/09/2015, 09:57

... Xuhua, Ms Sunita and, Mr Jobi and the rest of the lab mates for the valuable discussion and friendship and the present and former members of Functional Genomics Laboratory as well as Structural Biology ... purified to homogeneity by standard chromatographic techniques such as affinity chromatography, ion exchange chromatography and gel filtration chromatography The following introduction is adapted from ... molecules to monitor the production of amplified products during each cycle of the PCR reaction It is one of the technologies of the genomic age and has become the method of choice for detection of...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... Cloning, expression and purification The PRTFDC1 and HPRT gene was obtained from National Institute of Health Mammalian Gene Collection 4926 Fig Sequence alignment of PRTFDC1 (accession number: NP_064585), ... with a concentration of 0.5 mm and growth was allowed to continue overnight Cells were harvested by centrifugation at 3500 g for 20 and frozen at )80 °C Before purification, the cell pellet was ... side chain of Asp201, a phosphate ion and five water molecules The phosphate involved in this interaction is bound by main chain interactions with Lys76 and Gly77 and the side chain of Arg207 The...

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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... transcription of genes encoding cytokines is not sufficient to guarantee the appearance of functional proteins As reported by Kaspar and Gehrke [18] and Dinarello [19], stimulation of macrophages by ... These cells are a good model to study inflammatory processes, because, in contrast to HepG2 cells, expression of IL-1b in fibroblasts is significant and these cells are also important in inflammatory ... determination of the cellular localization of MCPIP HepG2 cells were transfected with a vector overexpressing MCPIP and with an empty vector (control) using Lipofectamine 2000, according to the...

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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

Ngày tải lên : 18/02/2014, 16:20

... Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and from O3 to the backbone N of ... bonds from N3 to the backbone O of Phe133, and from O4 to the backbone N and the side chain of Thr131, with the ribose moiety anchored by two hydrogen bonds, one from 2¢-OH to the side chain of ... activities of F133N and F133A were only 50 and 20% of that of the wild-type enzyme Similar to the situation with wild-type UpUMPK, addition of 0.5 and mm GTP resulted in no detectable activation of F133N...

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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

Ngày tải lên : 06/03/2014, 00:21

... factor-jB and the induction of pro-inflammatory production of cytokines, mainly interleukin (IL)-8 [12] The activation of a pro-inflammatory signalling cascade has been confirmed to be Nod1 cytoplasmic ... of interactions of this protein agrees with its functional relevance and localization studies, where it was found to enrich both in the inner and partially in the outer membrane fractions A model ... strand of a second monomer, allowing for the formation of the dimer The surface of interaction between monomers also involves portion of chains D and E of the two monomers, which are held together...

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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Ngày tải lên : 07/03/2014, 16:20

... function of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites A schematic representation of the ... Here we conﬁrm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p and Pex6p involves ... effects of deletion and point mutations in Pex1p and Pex6p for the interaction of the proteins and for their overall function in peroxisome biogenesis Our results demonstrate a different role of the...

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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

Ngày tải lên : 16/03/2014, 06:20

... a cycle of polymerization and depolymerization, isoform separation by hydroxyapatite chromatography, and a nal gel ltration step Actin cosedimentation and cross-linking assays Samples of actin ... as a loose network of thin bers with occasional formation of bundles (Fig 5A) Bundle formation differs between actin isoforms and is a function of polymer concentration and ionic strength [21] ... dimerization interface of Iba2o and the Zn2+ ions are additionally coordinated by His85 side chains of adjacent molecules There are two additional Zn2+ ions bound by the Glu64 and Asp107 side chains of...

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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

Ngày tải lên : 16/03/2014, 10:20

... Calculating proton uptake release and binding free energy taking into account ionization and conformation changes induced by proteininhibitor association: application to plasmepsin, cathepsin D and endothiapepsinpepstatin ... a dipole from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), ... the case of S36A, no relaxation at all The rst type of relaxation (structural relaxation), which involves only proton and side chain motions, is seen in D132 and R110 mutants Substitution of either...

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Báo cáo khóa học: Structural and functional comparison of 15S- and 15R-speciﬁc cyclooxygenases from the coral Plexaura homomalla potx

Ngày tải lên : 23/03/2014, 12:20

... of the C-15 stereochemistry of the products orientation of the cDNA insert in the vector was confirmed by digestion with XbaI and the presence of mutations by digestion with HpaI or VspI Experimental ... for helpful discussions This work was ¨ supported by Estonian Science Foundation Grants 5639 (to N.S.) and 5100 (to I.J.) and National Institutes of Health Grant GM-53638 (to A.R.B.) References ... PCR cloning and structural analysis We obtained P homomalla samples from the Bahamas and confirmed the 15S-configuration of the endogenous PGs by Ó FEBS 2004 Comparison of coral 15S- and 15R-cyclooxygenases...

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Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

Ngày tải lên : 24/03/2014, 03:21

... addition of EDTA to a ﬁnal concentration of 18 mM (not shown) No blue or red shift was observed in the emission spectra of either sample on addition of Ca2+ or EDTA Detection of dimerization To ... conditions and stained with Gelcode Blue Ó FEBS 2002 Characterization of smelt antifreeze protein (Eur J Biochem 269) 1221 Determination of molecular mass by gel-ﬁltration chromatography A TosoHaas ... glycosylation site in the N-terminal extension sequence [13] Therefore, the goal of this study was to investigate the structural and functional characteristics of smelt type II AFP The effects and...

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Báo cáo khoa học: Submembraneous microtubule cytoskeleton: biochemical and functional interplay of TRP channels with the cytoskeleton pot

Ngày tải lên : 30/03/2014, 10:20

... TRP channels also share functional links with the cytoskeleton by other means, namely cytotoxicity and cell death For example, activation of TRPV1 leads to the inhibition of protein synthesis and ... exploring how TRP channels affect the distribution, function and endogenous level of myosins Modulation of TRP ion channels activities by the cytoskeleton TRP channels can be modulated by Ca2+-dependent ... alteration of the cytoskeleton results in inhibition of TRP channel opening For example, the requirement for a functional cytoskeleton in the activation of TRP channels in store-mediated Ca2+ entry and...

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Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx

Ngày tải lên : 30/03/2014, 15:20

... neurodegeneration, and mutations in VCP have been observed to cause cytoplasmic vacuoles followed by cell death because of a dysfunctional second ATPase domain and inclusion body formation [120–123,127,135,136] ... horizontal lines from top to bottom: UIM sequences of the Pfam seed alignment including ﬁrst, second, and third UIMs of ataxin-3 homologues, UIM-like peptides from GGAs and Tom1, and related AP180 ... NMR solution structures of the UBL domain of hHR23A/B bound to a UIM peptide of S5a [99,119] could be used to model the complex of hHR23A/B and ataxin-3 Similarly, the complex of a UIM of ataxin-3...

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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc

Ngày tải lên : 22/06/2014, 06:20

... measured the rate of functional degradation of the antibiotics (both free and Gnps conjugated forms) by storing them at room temperature Both the forms of antibiotics were stored at room temperature ... activities were evaluated by measuring the area of zone of inhibition (diameter2) We used autoclaved water and only Gnps as negative control Results Production of Gnps on reduction with citrate or ... coli DH5a, M luteus and S aureus by comparing corresponding zone of inhibition (diameter2) In Fig 6, the zone of inhibition by agar well diffusion assay for E coli DH5a was shown to increase at a...

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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf

Ngày tải lên : 22/06/2014, 19:20

... measured the rate of functional degradation of the antibiotics (both free and Gnps conjugated forms) by storing them at room temperature Both the forms of antibiotics were stored at room temperature ... activities were evaluated by measuring the area of zone of inhibition (diameter2) We used autoclaved water and only Gnps as negative control Results Production of Gnps on reduction with citrate or ... coli DH5a, M luteus and S aureus by comparing corresponding zone of inhibition (diameter2) In Fig 6, the zone of inhibition by agar well diffusion assay for E coli DH5a was shown to increase at a...

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structural and functional organization of the synapse

Ngày tải lên : 06/07/2014, 15:30

... STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE Edited by Johannes W Hell University of Iowa Iowa City, IA, USA and Michael ... plasma membranes of the spine head and presynaptic bouton contains dense staining material, presumably composed of adhesion molecules and portions of receptors (d) Serial section between (c) and ... .205 Cyril Hanus and Michael D Ehlers Structure and Mechanism of Action of AMPA and Kainate Receptors 251 Mark L Mayer Cellular Biology of AMPA Receptor Trafficking and Synaptic Plasticity...

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Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf

Ngày tải lên : 08/08/2014, 14:20

... released from one of these clones by enzyme digestion was subcloned into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5’region of GS1a to ... 5’upstream region of the pine gene was able to drive gene expression in pine cotyledons To further characterize the function of the 5’upstream region of the pine GS1a gene, stable GUS expression was studied ... DNA-protein interactions in the 5’ region of GS1a gene from Scots pine We have carried out an in vitro study of interactions between nuclear factors from Scots pine cotyledons and an A/T-rich sequence...

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Báo cáo y học: "Structural and functional map of a bacterial nucleoid" doc

Ngày tải lên : 09/08/2014, 20:21

... Tavazoie and colleagues [3] opens up the possibility of studying, at a high resolution, the zones of the nucleoid occupied by the entire repertoire of transcription factors The next step should be to ... organizational center of the bacterial nucleoid, potentially also carrying out the important functional task of repression of silent DNA sequences by H-NS [21,22] Taking it further The work of Tavazoie ... 18:1766-1779 Woldringh CL, Jensen PR, Westerhoff HV: Structure and partitioning of bacterial DNA: determined by a balance of compaction and expansion forces? FEMS Microbiol Lett 1995, 131:235-242...

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Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot

Ngày tải lên : 10/08/2014, 05:21

... sedimentation coefficients (s), molar mass (M), and local concentrations (C) of different species were shown The local concentration of SV was from the discrete distribution of SV and that of SE was from ... distributions from global analysis of the three discrete species model by SEDPHAT according to eq The molar mass and sedimentation coefficients of the species were floated and fitted The residuals of ... the explanation of diverged functionality of truncated apoE isoforms Materials and methods Plasmids The construction of pET-apoE2, apoE3, apoE4, apoE3(72-166), and apoE4-(72-166) vectors were described...

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