stress responses of lactic acid bacteria in the context of species or genera

The roles of lactic acid bacteria in host inflammatory responses

Ngày tải lên : 13/09/2015, 19:50

... belong to lactic acid bacteria (LAB) which is an important group in maintaining human health The role of intestinal microflora, such as several strains of lactic acid bacteria (LAB) in priming the ... reinforce the barrier function of the intestinal mucosa, helping in the prevention of the attachment of pathogenic microorganisms and the entry of allergens Alteration of the microbial flora of the intestine ... role in the maintenance of the intestinal immune system There is accumulating evidence that xix the imbalance of the intestinal bacteria might cause inflammatory disorders such as allergy, inflammatory...

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Báo cáo y học: "Anti-inflammatory potential of a malleable matrix composed of fermented whey proteins and lactic acid bacteria in an atopic dermatitis model" pot

Ngày tải lên : 11/08/2014, 08:21

... pro-inflammatory cytokine following MPM consumption indicated its potential in the inhibition of development of inflammatory disease and reduction of its intensity Moreover, Page of 10 (page number not for ... the inhibition of development of inflammation following an inflammatory stimulus The anti-inflammatory potential of MPM has been confirmed in these studies with the murine ACD model This model of ... TNFα in healthy rat [33] This cytokine is very important in the development of the ACD disease and contributes in the amplification of inflammatory reaction [46] The reduction of this pro-inflammatory...

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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

Ngày tải lên : 07/03/2014, 04:20

... in maintaining the solubility of the Hsp25 oligomer Similarly, the introduction of hydrophobicity into the C-terminal extension of aA-crystallin results in a decrease in the thermostability of ... protein indicates that the structure of the N-terminal domain is maintained, at least in the vicinity of the tryptophan residues The increase in exposed clustered hydrophobicity observed for the ... at the beginning of the a-crystallin domain corresponding to residues 70–88 in aA-crystallin [63,64] The changes in tertiary structure observed for the mutants, as evidenced by the alteration in...

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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

Ngày tải lên : 16/03/2014, 10:20

... with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ring ... against ammonium bicarbonate a small proportion of dimers were formed, even in the case of the wild-type protein as indicated by ESI-MS or SDS PAGE in the absence of reducing agents The proportion ... energies of the original resiDG i;j due i in the native protein with the rest of the residues, self DDG k mutị are the changes of the self energies of the residues k that change either their ionization...

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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

Ngày tải lên : 23/03/2014, 03:20

... 27 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 amino amino amino amino amino 15 15 15 15 15 acids acids acids acids acids amino amino amino amino amino acids ⁄ 237 acids ⁄ 237 acids ... Because the carboxylate of this Asp217 (194, chymotrypsin numbering) is involved in the formation of a salt bridge with the N-terminal Ile of the mature enzyme for completing the formation of Fig ... C-terminal segment of the inhibitor SGTI, whereas Loop60 plays a role in the formation of the S1¢–P1¢ interaction [8] Apart from the largest loops of P argus (Fig 3D), considering the amino acid substitutions...

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Báo cáo khoa học: Identiﬁcation of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockﬁsh Sebastes schlegeli pot

Ngày tải lên : 30/03/2014, 10:20

... glycoprotein consisting of 75-kDa subunits by SDS ⁄ PAGE, partly because of the scarcity of the protein of interest In this study therefore we isolated the antibacterial protein, SSAP, from the skin ... superfamily lectins via its sialylated glycan moiety results in the production of locally high concentrations of H2O2 in or near the binding interface The role of the glycosyl substituents of LAOs in biological ... tail Antibacterial LAO of rockﬁsh skin mucus at nucleotide 2027 An ORF is composed of 1662 bp, encoding a precursor protein of 554 amino -acid residues from the putative initiating Met to the putative...

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Analysis and practical implementation of a model for combined growth and metabolite production of lactic acid bacteria

Ngày tải lên : 05/05/2014, 08:44

... fully determined when the total amount of lactic acid in the medium and the buffering capacity of the medium are known The amount of undissociated lactic acid is then further related to the total ... Furthermore, when the pH and /or buffer amount further increase, the intersection of this line with the LaHtot-axis increases, or otherwise said, the straight line is shifted to the right The pH ... to the dissociation constant of lactic acid) (Fig 2a) or when no buffer BuHa/BuMa is present (Fig 2d), the curve is more or less a straight line passing through the origin In case of a higher initial...

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Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract pot

Ngày tải lên : 07/07/2014, 19:20

... 2006) The indicator strains used in this study were gram-negative strains as the main pathogenic microorganisms in the intestinal tract of chicken such as Escherichia coli, and Salmonella sp In ... 1) The 548 isolates of LAB were isolated from crop, small intestine, large intestine and caecum of chicken intestinal tracts The number of LAB isolated from each organ of broilers or Thai indigenous ... 2.5) containing pepsin (3 mg ml-1) (data not shown) In comparison to the acid tolerance of the Lactobacillus species isolated from the gastrointestinal tracts of swine and chicken, Lin et al (2007)...

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Báo cáo khoa học: "Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use" ppsx

Ngày tải lên : 07/08/2014, 18:20

... Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic use Acida Bileb BSHc Streptococcus ... hydrolase activity in the MRS plate media containing taurodeoxycholic acid d Number of resistant strains/Number of selected strains (%) solution was also incubated at 37oC for 24 hrs for the control ... Studies of a surfactant and cholesterolemia in the Massai Am J Clin Nutr 1974, 27, 464469 19 Oh CY, Lee WK Cholesterol lowering effect of lactic acid bacteria isolated from the human intestine Korean...

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Báo cáo y học: "Lack of association of a variable number of aspartic acid residues in the asporin gene with osteoarthritis susceptibility: case-control studies in Spanish Caucasians" potx

Ngày tải lên : 09/08/2014, 07:20

... recipient of a scholarship of the National Program for the Training of University Professors of the Spanish Ministry of Education AG was supported by the Instituto de Salud Carlos III (Spain) References ... effect of variation in the number of D residues in ASPN in hip OA, knee OA or hand OA susceptibility in Spanish Caucasians This result reinforces the overall lack of association reported in other ... confirmation in the same ethnic population Therefore, although it remains possible that ASPN could be a crucial modulator in OA by fine-tuning transforming growth factor beta in the repair of damaged...

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Báo cáo y học: " Conserved charged amino acid residues in the extracellular region of sodium/iodide symporter are critical for iodide transport activity" potx

Ngày tải lên : 10/08/2014, 05:21

... [19-23] Therefore, herein we analyzed the critical roles of amino acid residues in the extracellular segments of NIS, and our findings indicated that these residues affected the iodide transport ... iodide uptake activity of NIS [13] Furthermore, arginine residues of organic anion transporter influence the binding of glutarate and interact with chloride [28] Arg-211 residue of rabbit proton-coupled ... might affect the entry or binding of iodide ions It is interesting to find that residues (Glu-79, Arg-82, Asp-233, Asp237, Arg-239, and Arg-241) involved in the entry or binding of iodide ions...

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Báo cáo y học: " Role of taurine on acid secretion in the rat stomach" ppsx

Ngày tải lên : 10/08/2014, 05:21

... plexus of the enteric nervous system in the stomach Taurinergic neurons in the muscle layer of the gastrointestinal (GI) tract might be involved in motility of the GI tract and the function of endocrine ... magnification of the area in (A) showing taurine-positive processes in the antrum (E) A higher magnification of the area in (B) showing taurinepositive processes in the body Taurine-immunoreactive ... taurine-containing cells in the rat stomach is confirmed, as indicated by CSAD- and taurine-positive cells We found the presence of taurine-containing cells and taurine-induced acid secretion in...

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Báo cáo y học: "Amino acid substitutions in the E2 glycoprotein of Sindbis-like virus XJ-160 confer the ability to undergo heparan sulfate-dependent infection of mouse embryonic fibroblasts" docx

Ngày tải lên : 12/08/2014, 01:21

... HSdependent in infection of cells due to lacking of the two basic amino acids in the second HS-binding domain Specific interaction of the peptide containing 145-150 aa from YN87448 E2 gene with heparin ... data demonstrate the importance of positively charged residues in the HS-binding domain of E2 in mediating SINV infection Increased binding of alphaviruses to HS usually results in smaller plaque ... and no binding of the corresponding peptide from the of XJ-160 E2 gene to heparin further strengthened this speculation However, the effects of E2-146Tyr and E2-149Asn on HS binding of Sindbis...

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Báo cáo y học: " Second site escape of a T20-dependent HIV-1 variant by a single amino acid change in the CD4 binding region of the envelope glycoprotein" doc

Ngày tải lên : 13/08/2014, 09:20

... domain Binding of the surface subunit gp120 to CD4 and a co-receptor on the T-cell surface triggers conformational changes in the Env complex, leading to the insertion of the hydrophobic N-terminal ... replication of the wild-type was strongly inhibited by T20 Introduction of the positive arginine (G431R) resulted in increased replication of the wild-type in the absence of T20 This may be due to the ... observed in the context of the GIA-SKY virus However, when an alternative neutral amino acid (alanine which is the most analogous amino acid to the endogenous glycine; G431A) was introduced into the...

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Báo cáo y học: "Use of the probiotic Lactobacillus plantarum 299 to reduce pathogenic bacteria in the oropharynx of intubated patients: a randomised controlled open pilot study" pps

Ngày tải lên : 13/08/2014, 11:23

... the findings emerging enteric bacteria Distribution of the findings ofof emerging enteric bacteria On the first days of ICU care, identified emerging enteric species were twice as many in the ... substantially to the analysis of the results of the bacterial cultures and completion of the manuscript BJ took part in planning of the study and in finalising the manuscript AL helped plan the study ... gradual decrease in the number of patients remaining in the study (similar in both groups), new cases of tracheal infection appeared in the latter part of the study period, primarily in the control...

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fermentation of mango juice by lactic acid bacteria

Ngày tải lên : 06/10/2015, 12:57

... Note: The maximum score is The data were the average of sensory evaluators In short, from the results of pH, Brix, lactic acid content, the density of bacteria and the results of the sensory evaluation ... Count density of lactic acid bacteria: plate counting method Monitor the indicators: Brix, pH, level of lactic acid after fermentation and the density of lactic acid bacteria Analyze the data with ... Figure 10 The change of acid content according to inoculum density, temperature and time of fermentation In general, the acid content increased when incubation time increased Acid content of the samples...

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fermentation of carrot juice by lactic acid bacteria

Ngày tải lên : 06/10/2015, 13:04

... Isolation of lactic acid bacteria All pure isolates were classically defined by observing macro and micro morphology As the result, isolates of lactic acid bacteria were isolated from kinds of carrot ... contained 36 mL carrot juice + mL of inoculum) Each tube represented a single sample and the experiments were performed in triplication The lactic acid fermentation was performed in 37ºC for 48 ... log cell/mL of the bacterial inoculum, for 24 hours of fermentation time and at 37ºC The result of storage testing showed that suitable temperature for product storage was 4-6ºC, sensory evaluation...

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fermentation of dragon fruit juice by lactic acid bacteria

Ngày tải lên : 06/10/2015, 13:04

... (more than per 10 points) In particular, the 9%-concentration-rate sample got the highest score due to harmonious flavor In summary, from the results of the acid index, the concentration of bacteria ... due to the production of lactic acid during development of bacteria Concentration of lactic acid produced from A strain (1.38%) and Pro strain (1.36%) were higher than other strain’s and they were ... determine the isolated bacteria strains at genus level The results showed that: - Gram staining: Bacteria strains remained purple color after staining crystal violet, thus they were Gram-positive bacteria...

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fermentation of pineapple juice by lactic acid bacteria

Ngày tải lên : 06/10/2015, 13:05

... and reduce lactic acid Therefore, lactic acid content in these simples was high On the other hand, storage of samples at – 6ºC gave high sensory score because they still had the same favorable smell ... That satisfied the requirement of probiotic product There for, all of these LAB strains could use to apply in producing of fermented pineapple juice Besides, acid lactic content of L acidophillus ... converting all sugar into lactic acid inhibited the bacterial growth and development Besides, lactic acid itself could inhibit the development of bacteria (Nguyen Thi Hien, 2006) In contrast, the...

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PRESSURE, STRESS, STRAIN AND LOANGDING FORM USED IN THE CALCULATION OF CHEMICAL EQUIPMENT

Ngày tải lên : 08/06/2016, 11:17

... called stress categories Types of Stress Define a direction of stress or relate to the application of the load Tensile Compressive Shear Bending Bearing Axial Discontinuity Membrane Principal ... based on the weakest part in the new and cold condition, and all other loadings are not taken into consideration 2 STRESS & STRAIN • The combined stresses due to a combination of loads acting simultaneously ... Secondary bending stress Peak stress, F STRAIN LOADINGS • Loadings or forces are the "causes" of stresses in pressure vessels • Type of loading: o Steady loads o Non-steady loads o General loads...

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