specificity of real time hybridization probe rt pcr
Development of multivariate curve resolution and associated system identification tools for IR emission, chiroptical, and far infrared and far raman spectroscopies
Ngày tải lên :
12/09/2015, 08:20
... DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMICAL & BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT This thesis is the result of four and half years of work ... (1998), many of the methods employed in chemometrics are based on the concept of soft modeling It is worthy to note that soft modeling provides a more realistic framework for many of the chemometric ... profiles 10 Chapter 2.2.1 History of SMCR The starting point of SMCR goes back to the realization that the number of chemical components in a mixture might be determined by the matrix rank of...
- 260
- 402
- 0
Tài liệu Selecting the Top n Rows in a DataTable doc
Ngày tải lên :
14/12/2013, 18:16
... MessageBoxIcon.Stop); return; } // Clear the filter on the view dv.RowFilter = ""; // Sort the view descending on the top n field dv.Sort = topNFieldName + " DESC"; // Create a filter for all records with a ... the nth value // Eliminate enough rows from the bottom of the dv using a filter on // the primary key to return the correct number (top n) of values bool refilter = false; // Iterate over all records ... Freight value involves several steps First, sort the DataView on the Freight field in descending order; this places the top n records at the top of the view Next, get the Freight value for the...
- 4
- 332
- 0
Tài liệu Murray N. Rothbard - History Of Money And Banking In The United States (pdf) pptx
Ngày tải lên :
17/01/2014, 02:20
... with judgments of value, with the choice of ends and of the means resorted to for the attainment of these ends, and with the valuation of the outcome of actions performed.21 Furthermore, whether ... Mises’s words, “are the starting point of a specific mode of reflection, of the specific understanding of the historical sciences of human action.” Equipped with the method of “specific understanding,” ... contribution of the railroad to the growth of real GNP in the United States? Or, what has been the effect of the creation of the Federal Reserve System on the stability of the price level and real output?...
- 510
- 1.2K
- 0
Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx
Ngày tải lên :
17/02/2014, 14:20
... m2, respectively If they are a distance d apart, determine the force of gravity acting between them Compare this result with the weight of each particle Units Used: G = 66.73 × 10 − 12 m kg⋅ ... No portion of this material may be reproduced, in any form or by any means, without permission in writing from the publisher Engineering Mechanics - Statics Chapter Problem 1-16 Two particles ... portion of this material may be reproduced, in any form or by any means, without permission in writing from the publisher Engineering Mechanics - Statics Chapter Problem 1-4 Represent each of...
- 1.1K
- 1.1K
- 2
Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... we further show the importance of PKA-mediated phosphorylation in the mitochondrial targeting of CYP2E1 Most importantly, substrate conversion by mitochondria-targeted CYPs was fully supported ... release of porin started at a much lower concentration of 0.025% These results further support the possibility that mitochondria-associated CYP2E1 is located inside the innermembrane compartment ... localization of intact CYP2E1 in yeast cells Because of the existing ambiguity in the literature on the nature and extent of CYP2E1 import into mitochondria, we first established the relative purity of...
- 16
- 650
- 0
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... heavily in the presence of Cu2+ at pH and to a lower extent at pH Therefore, no comparison of the stability of the tertiary structure to the effect of Cu2+ could be made Influence of copper on oligomerization, ... at pH [5,6] In the present series of experiments we compared fibrillation of stefin B wildtype (E31 isoform), of stefin B variant (Y31 isoform) and the mutant P79S of the variant, all at pH 5.0 with ... of the protein -1 -2 Stefin B pH5 kcal/mole of injectant Near UV CD spectra are a better measure of protein tertiary structure and thus the solution conformation There are cases where the tertiary...
- 14
- 586
- 0
Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc
Ngày tải lên :
19/02/2014, 07:20
... Reverse 1534 mao, cloning mao, cloning sad, cloning sad, cloning mao, RT- PCR mao, RT- PCR sad, RT- PCR sad, RT- PCR mabO, RT- PCR mabO, RT- PCR FEBS Journal 273 (2006) 1528–1536 ª 2006 The Authors Journal ... Arrows indicate the position of primers employed in the PCR amplification of gene fragments and the numbers the size in basepair of the amplified DNA fragment (B) RT- PCR of RNA derived from A nicotinovorans ... bioremediation of nicotine waste, but also in the supply of nicotine derivatives as starting materials for the synthesis of new products of industrial and pharmaceutical importance [3,4] Construction of...
- 9
- 524
- 0
Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
Ngày tải lên :
19/02/2014, 13:20
... independent of the thermodynamic and kinetic properties of the binding process and is solely based on the detectability of the peptides of interest We have used this method to show that binding of fosfomycin ... with evidence from studies of small angle X-ray scattering [14] and the protective effect of UDPNAG on proteolysis of MurA [15] Here we report the thermodynamic profile of UDPNAG binding to two ... of UDP-N-acetylmuramic acid) (A) and inactivation mechanism of MurA by fosfomycin as a result of the covalent linkage between Cys115 of MurA and fosfomycin (B) Fig Structural representation of...
- 9
- 707
- 0
Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
Ngày tải lên :
19/02/2014, 17:20
... regulation of the transport activity and surface expression of neurotransmitter transporters [10,17] Functional expression of the GABA transporter is abolished by tunicamycin, a potent inhibitor of N-glycosylation ... important for the GABA transport activity 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal structures of the N-glycans of GAT1, ... N-glycans of oligomannosidic type 1627 Role of N-glycosylation and N-glycan trimming of GAT1 G Cai et al Expression of N-glycosylation mutants on the plasma membrane of CHO cells The expression of NNN...
- 14
- 654
- 0
Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
Ngày tải lên :
20/02/2014, 11:20
... apparent molecular mass of 100 kDa The Lon protease may be structurally modified by oxidation, which in turn could reduce its activity In support of our assertion, recent reports claiming that carbonylated ... terminated by adding 30 lL of 40% trichloroacetic acid and 50 lL of 3% BSA After centrifugation at 15 000 g for 30 min, 100 lL of M sodium borate was then added to 80 lL of supernatant Fluorescence ... only 51% of that of adult (10-months) rats Such a decrease in matrix enzyme activity cannot result from the disruption sensitivity of mitochondria as we observed roughly the same pool of disrupted...
- 8
- 412
- 0
Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
Ngày tải lên :
07/03/2014, 05:20
... degradation of FR-linker–GFP by wild-type ClpA required the addition of ClpS 1402 (Fig 2D) Importantly, like wild-type ClpA, RR ⁄ AA was also able to support the ClpS-dependent degradation of FR-linker–GFP ... ClpP-mediated degradation of a SsrA tagged peptide (50 lM) was monitored in the absence of ClpA (ClpP) and the presence of wild-type (ClpA) or mutant (RR ⁄ AA) proteins (C) Time course of a-casein degradation ... addition of ATP (lane 1), in the presence of 160 pmol dWB ClpA6 without (lane 2) or with addition of mM ATP (lane 3), or in the presence of 160 pmol RR ⁄ dWB ClpA6 without (lane 4) or with addition of...
- 11
- 434
- 0
Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
Ngày tải lên :
07/03/2014, 17:20
... Binding to supported lipid membranes Fig Insertion of stefins into DOPC and DOPG monolayers (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of mNÆm)1 The proteins ... amyloid-b peptide (A-b) that recognizes the structure of the prefibrillar oligomers of a number of amyloidogenic proteins [9], further supporting a generic mechanism A mechanism for toxicity was ... oligomers of vesicles made of acidic phospholipids resembles pore formation by A-b [27] and liposome permeabilization of a-synuclein [43] The toxic activity exerted by prefibrillar forms of stefin...
- 10
- 476
- 0
Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
Ngày tải lên :
07/03/2014, 21:20
... necessity of Arg46 in the V1aR it was important to identify the features of the arginyl side-chain that supported high affinity agonist binding A comprehensive approach was undertaken in which each of ... activation of the V1aR Discussion Receptors which mediate the effects of the neurohypophysial peptide hormone family are structurally and pharma- Fig Effect of substitution of Arg46 of the V1aR ... switch of the V1aR (Eur J Biochem 270) 4683 Results The effect of systematic substitution of Arg46 on agonist binding The structural requirements of the residue at position 46 in the N-terminus of...
- 8
- 487
- 0
Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
Ngày tải lên :
08/03/2014, 08:20
... from the NBD than ATP Rapid dissociation of hydrolysis product may be expected as part of the kinetics of the transport cycle of the intact transporter Furthermore, the data demon- strate that ... appropriate choice of template as it shares a high degree of sequence identity to the NBDs of P-glycoprotein, and is a member of the same subfamily of ABC transporters (ABCB) However, the reported crystal ... properties of the NBDs, rather than of their interactions with the TMDs (e.g [50] and see introduction for further references) Recent structural determinations of both intact ABC transporters...
- 10
- 386
- 0
Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... evaluation of the function of the three serine mutants, the kinetic properties of the mutant enzymes were compared to those of the parent enzyme, P-DN42 As shown in Table 1, the Km values of all ... UDP Ó FEBS 2002 portion of the sugar donor Recent crystallographic studies on glycosyltransferases indicate that several interactions are involved in binding of the UDP portion of the sugar donors ... the basic catalytic function of the GalNAc-transferases, but rather are important in defining the catalytic properties of specific isozymes In fact, the interaction of UDP-GalNAc with GalNAc-T5,...
- 9
- 435
- 0
Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot
Ngày tải lên :
16/03/2014, 13:20
... development 3288 Fig Topological model of Kv3.1 and amino acid sequences of the S1–S2 linker of Kvs (A) Topology of a Kv3.1 monomeric unit Black circles represent the Asn of native N-glycosylation consensus ... currents were placed on top of each other (B) Rise times and (C) activation time constants are shown for wild-type Kv3.1 (d) and N220Q ⁄ N229Q (n) Rise times represent the time required for the current ... kinetics of wild-type Kv3.1 (time constant deactivation, soff is 2.4 ± 0.9 at ) 40 mV, n ¼ 3) and N220Q ⁄ N229Q (soff is 3.6 ± 0.3 at ) 40 mV, n ¼ 3) were rapid, and similar to those previously reported...
- 14
- 406
- 0
Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
Ngày tải lên :
16/03/2014, 16:20
... efficiency of N-myristoylation of various cellular proteins may arise in part because of differences in the presentation of Gly2 dictated by interactions among the residues at positions 3, 6, and of ... 1.7% of all proteins [17] S cerevisiae Nmt1p is the best studied of the known NMTs The X-ray structure of a binary complex of Nmt1p with bound myristoyl-CoA has been determined [34] A structure of ... presence of [3H]leucine, [35S]methionine or [3H]myristic acid under conditions recommended by the manufacturer The mixture (composed of 17.5 lL of rabbit reticulocyte lysate, 0.5 lL of mM leucine-...
- 12
- 512
- 0
Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
Ngày tải lên :
16/03/2014, 18:20
... role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription Ó FEBS 2004 Regulation of the presenilin gene (Eur J Biochem ... for flexibility in the utilization of the promoter Analysis of the start sites of the major species of PS1 mRNAs present in brain and human placenta revealed a set of 5¢ ends, all present between ... J Biochem 271) 4489 A Fig Effect of PS1 promoter point mutations on the efficiency of transcription initiation and 23 the position of the start site(s) (A) Effect of point mutations in Ets and Sp1...
- 10
- 492
- 0
Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
Ngày tải lên :
16/03/2014, 23:20
... incubation time at °C (r) and 37 °C (d) is reported for each dimeric protein intrinsic flexibility of this tract of the protein Yet, in the crystallographic structure of a dimeric variant of human ... Insertion of the correct mutations was confirmed by DNA sequencing Recovery of proteins Materials and methods Construction of mBS mutants Site-directed mutagenesis was performed by a megaprimer PCR ... structure of mBS [8] To allow a more accurate evaluation of the effect of single point mutations on the solution structure of monomeric derivatives, we analysed the 2D NMR spectra of the different...
- 7
- 404
- 0
Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
Ngày tải lên :
22/03/2014, 21:20
... is mainly involved in the synthesis of small chains of this fraction Furthermore, SSIV has been found recently to be involved in the control of the number of starch granules and starch granule ... reported that different starch biosynthetic enzymes (including several SS isoforms) are capable of associating in a multisubunit complex, and that these interactions may be of physiological importance ... N-terminal region of SSIII can interact with SSI [13] The possible regulatory role of this protein makes this isoform a potential target for the manipulation of the level and quality of plant starch...
- 13
- 457
- 0