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Development of molecular diagnostics and antiviral therapy against enterovirus 71
Ngày tải lên :
12/09/2015, 08:20
... Development of real- time Hybridization probe RT-PCR 3.2.2.1 Specificity of real- time Hybridization probe RT-PCR 3.2.2.2 Quantitative analysis of real- time Hybridization probe RT-PCR 3.2.2.3 Detection of ... specimens by real- time Hybridization probe RT-PCR 3.2.3 Development of real- time TaqMan RT-PCR 3.2.3.1 Specificity of real- time TaqMan RT-PCR 3.2.3.2 Quantitative analysis of real- time TaqMan ... specific probe hybridization Real- time PCR 1.3.5.1 Advantages of real- time PCR over conventional PCR 1.3.5.2 Approaches to real- time PCR 19 21 21 22 23 23 26 27 27 29 iii 1.3.5.3 Detection of enteroviruses...
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Tài liệu Báo cáo khoa học: "Reactive Content Selection in the Generation of Real-time Soccer Commentary" pdf
Ngày tải lên :
20/02/2014, 18:20
... in MIKE to produce a real time narration 2.2 What, How a n d When-to-Say The previous section pointed out that contents should be uttered at the right time; that is, real time discourse systems ... a real- time game log1 of a very high quality, sending information on the positions of the players and the ball to a monitoring program every 100msec Specifically, this information consists of: ... delete time or utter Figure 4: An example transformation of importance of a proposition put can be is in English, French or Japanese To produce speech, MIKE uses off the shelf text-to-speech software...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
Ngày tải lên :
19/06/2014, 08:20
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real- time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... Analysis of HA and quantitative real- time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real- time PCR data employed for the determination of JC ... end of the cycle Real- time PCR amplification data were analyzed using the Bio-Rad iCycler iQ™ Multicolor Real- Time PCR Optical System Software Version 3.1 A standard curve for the quantitation of...
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Báo cáo hóa học: " Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia" pptx
Ngày tải lên :
20/06/2014, 01:20
... the commonest cause of death in adult males in The Gambia [4] Detection of serological markers is the mainstay of diagnosis of HBV infection and the most reliable marker of HBV carriage is HBV ... quantification of virus load as a measure of replication of HBV in patients with chronic liver disease Prior to the development of the polymerase chain reaction (PCR) a number of hybridisation ... so the majority of the HBV carriers were over 20 years Levels of viral load was compared with HB sero-status Real- time quantitative PCR for HBV DNA DNA was extracted from 200 µL of serum using...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
Ngày tải lên :
20/06/2014, 04:20
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real- time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... Analysis of HA and quantitative real- time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real- time PCR data employed for the determination of JC ... end of the cycle Real- time PCR amplification data were analyzed using the Bio-Rad iCycler iQ™ Multicolor Real- Time PCR Optical System Software Version 3.1 A standard curve for the quantitation of...
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báo cáo khoa học: " Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data" potx
Ngày tải lên :
12/08/2014, 03:21
... Accurate normalization of real- time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biol 2002, 3:7 Gachon CMA, Charrier B: Real- time PCR: what relevance ... decade of improvements in quantification of gene expression and internal standard selection Biotechnology Advances 2009, 27(4):323-333 12 Andersen CLJJ, Orntoft TF: Normalization of real- time quantitative ... quantitative real- time PCR Vet Parasitol 2003, 118:169-174 45 Robinson TLSI, Sutherland J: Validation of candidate bovine reference genes for use with real- time PCR Vet Immunol and Immunopathol 2007, 115: 160-165...
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Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
Ngày tải lên :
12/08/2014, 04:21
... sequencing and for evaluation of sensitivity of the real- time PCR JE, WN and CHK viruses were used to evaluate the specificity of the assay Infection and maintenance of cell cultures Vero cells ... sets for detection of all four serotypes of DENV [11] In the present study, we describe the development of a DENVspecific TaqMan based real- time PCR for detection and quantitation of all four serotypes ... surveillance of vector population and detection of DENV in field caught mosquitoes Real- time PCR because of its high sensitivity could be useful in such surveillance and provide early warning of a possible...
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Báo cáo y học: "Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection" pptx
Ngày tải lên :
12/08/2014, 23:23
... However, real- time PCR offers a rapid, sensitive and highly reliable new technique that may improve our understanding of the epidemiology of severe LRTIs Conclusion Real- time PCR for a broad range of ... additional advantage of real- time PCR over standard-format PCR is that it allows high-throughput screening of patient samples for the presence of many different pathogens Our real- time PCR assays ... the yield of viral culture depends on the quality of sampling, the correct transport and storage of samples, and the type of cells used In addition, the technique has a turnaround time of several...
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Báo cáo y học: "The effect of real-time CPR feedback and post event debriefing on patient and processes focused outcomes: A cohort study: trial protocol" ppsx
Ngày tải lên :
13/08/2014, 23:20
... performance [16] Systems of post-event debriefing of the cardiac arrest team of real life resuscitation atempts were associated with improved: knowledge; adherence to guidelines; quality of CPR and a significantly ... performance category of patients Page of 17 at discharge The process focussed outcomes focus on both quality of CPR and team factors Quality of CPR outcomes are: quality of 2222 call; chest compression ... and confusion at a time when they are already burdended by either the loss of a loved one or shock of a sudden deterioation in health status Our assessment of the burdens of approaching relatives...
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Báo cáo y học: "Base relative quantification framework and software for management and automated analysis of real-time quantitative PCR data" potx
Ngày tải lên :
14/08/2014, 17:22
... formulas N, number of replicates i; g, number of genes j; c, number of IRCs m, m'; r, number of runs l, l'; s, number of samples k; f, number of reference genes p, p'; h, number of standard curve ... under the form of XML files [15] Data can be imported from a number of data formats Two standards (qBase internal format and RDML (Real- time PCR Data Markup Language)) and a number of instrument ... flexibility, and user friendliness of operation Our quantification framework and software fit exactly in current thinking that places emphasis on getting every step of a realtime PCR assay right (such...
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Improved symbolic model checking of real time systems
Ngày tải lên :
09/09/2015, 11:10
... correctness of real- time systems In this chapter, we give an overview of real- time model checking and discuss the research gaps Then, we present the objectives of this thesis 1.1 Real- time Model ... checkers on timed automata Similarly, Sun et al [95] proposed Stateful Timed CSP, an extension of Timed CSP, to model hierarchical realtime systems Stateful Timed CSP extends Timed CSP with timed process ... constraints Timed safety automata [50] are an variant of timed automata with state invariants to limit the duration of the timed automaton staying at a certain state However, both timed automata and timed...
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Interactive design space exploration of real time embedded systems
Ngày tải lên :
11/09/2015, 16:04
... Hardware/Software Partitioning We develop an efficient scheme for design space exploration in the context of hardware/software co-design of real- time systems Such systems nowadays consist of a heterogeneous ... to the time at which the task begins execution, or can be relative to the ready -time of another job of the same task Therefore, a constraint like the ready-times of two consecutive jobs of a task ... triggerings of the source vertex should be separated by at least P (T ) units of time, called the period of the task graph Therefore, a sequence of vertices v1 , v2 , , vk getting triggered at time...
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Dynamic scheduling of real time control systems
Ngày tải lên :
04/10/2015, 17:06
... function of sampling frequency, but also a function of time ∞ Jd (t, f ) = k=t·f (y(k/f ) − r(k/f ))2 , f (4.8) where Jd (t, f ) considers PI in the time period of current time t to ∞, instead of considering ... the desired frequency of the closed loop system A rule of thumb to decide the sampling interval for discrete -time control is that one should sample to 10 times per rise time of the closed loop system, ... attributes include the task release time, the task period, the task execution time, the response time and the task deadline • The release time is the moment of time when the task becomes eligible...
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Evaluation of real time methods for epidemic forecasting 2
Ngày tải lên :
12/10/2015, 17:34
... Effects of a on C(t) a=0.5 a=1 a=2 −5 Effects of K on I(t) 0.0 0.1 0.2 0.3 0.4 0.5 t Effects of r on I(t) r=0.5 r=1 r=2 0.1 0.2 0.3 0.4 K=0.5 K=1 K=2 0.0 I(t) 15 tm=0 tm=5 tm=10 −5 10 t Effects of ... −5 10 15 10 15 t Effects of a on I(t) a=0.5 a=1 a=2 0.2 0.1 0.0 0.0 0.1 0.2 0.3 10 t Effects of tm on C(t) −5 I(t) r=0.5 r=1 r=2 0.3 C(t) 0.0 0.2 0.4 0.6 0.8 1.0 Effects of r on C(t) 0.0 0.2 0.4 ... r=1 r=2 0.3 C(t) 0.0 0.2 0.4 0.6 0.8 1.0 Effects of r on C(t) 0.0 0.2 0.4 0.6 0.8 1.0 2.0 Effects of K on C(t) K=0.5 K=1 K=2 −5 ❢r♦♠ ❘✐❝❤❛r❞s ♠♦❞❡❧ ✇✐t❤ ♣❛r❛♠❡t❡rs ✉♥❧❡ss ♦t❤❡r✇✐s❡ st❛t❡❞ 0.5 C(t)...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
Ngày tải lên :
05/09/2013, 10:15
... Quantification of microcystin-degrading bacteria by real- time PCR The standard curve was generated from serial 10-fold dilutions of DNA carrying the mlrA of strain C-1 The quantification of microcystin-degrading ... Dr Fujimoto from Tokyo University of Agriculture ** Biofilm samples Samples of biofilm (10 g) were taken from a biological contact material, honeycomb catalyst, of a practical biological treatment ... reactions required µL of extracted DNA, 10 pmol of each primer, 30 pmol of TaqMan probe, 25 µL of TaqMan Gene Expression Master Mix (Applied Biosystems Co USA), made up to a final volume of 50 µL using...
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