role of stem cell imaging in regenerative medicine gabriella passacquale and kishore bhakoo
Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 1) ppt
Ngày tải lên :
07/07/2014, 01:20
... Sources of Stem Cells for Tissue Repair Different types of stem cells include embryonic stem (ES) cells, umbilical cord blood stem cells, organ-specific somatic stem cells (e.g., neural stem cells ... These include development of methods for reliably generating large numbers of specific cell types, minimizing the risk of tumor formation or proliferation of inappropriate cell types, ensuring ... for stem cell replacement therapy Regardless of the source of the stem cells used in regenerative strategies, a number of generic problems must be overcome for the development of successful clinical...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 2) pps
Ngày tải lên :
07/07/2014, 01:20
... of a variety of different cell types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose stem cells Bone marrow stem cells in particular have been examined in clinical ... differentiate into the desired cell types In addition to these strategies for cell replacement, the ex vivo or in situ generation of tissues provides an alternative means of tissue engineering (Chap 69) Stem ... for cell replacement have been considered (Fig 67-1): (1) injection of stem cells directly into the damaged organ or into the circulation, allowing them to "home" into the damaged tissue; (2) in...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 3) pot
Ngày tải lên :
07/07/2014, 01:20
... development of dyskinesia Transplantation of stem cell derived dopamine-producing cells offers a number of potential advantages over fetal transplants, including the ability of stem cells to migrate and ... Transdifferentiation of bone marrow and adipose stem cells into neural stem cells, and vice versa, has been reported, and clinical trials of such cells have begun for a number of neurologic disorders Clinical ... Potential sources of stem cells include endogenous liver stem cells (such as oval cells), ES cells, bone marrow cells, and umbilical cord blood cells Although a series of studies in humans as well...
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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 4) pot
Ngày tải lên :
07/07/2014, 01:20
... applications in regenerative medicine and cancer therapies Clin Pharmacol Ther 82(3):252, 2007 [PMID: 17671448] National Institutes of Health: Stem cell information page URL: http://stemcells.nih.gov/index.asp ... neuroscience and cognitive research, in vitro fertilization, and prenatal genetic testing From these and other precedents, we learn the importance of understanding and testing fundamental biology in the ... et al: Stem Cells and the Future of Regenerative Medicine Washington, D.C., National Academies Press, 2002 Holland S et al: The Human Embryonic Stem Cell Debate: Science, Ethics and Public Policy...
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Tài liệu Role of diffusion-weighted imaging in the diagnosis of gynecological diseases pdf
Ngày tải lên :
13/02/2014, 06:20
... Functional MR imaging of the female pelvis J Magn Reson Imaging 25:1101–1112 25 Koyama T, Tamai K, Togashi K (2006) Current status of body MR imaging: fast MR imaging and diffusionweighted imaging Int ... may also show increased intensity, the role of DWI and ADC in distinguishing between benign and malignant lymph nodes may be limited Lin et al [62] reported the combination of size and relative ... echo-planar MR imaging and ADC mapping in the differential diagnosis of ovarian cystic masses: usefulness of detecting keratinoid substances in mature cystic teratomas J Magn Reson Imaging 22:271–278...
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Potential applications of chitosan based hydrogels in regenerative medicine
Ngày tải lên :
10/09/2015, 09:23
... attachment study Following the rinsing, each cell- seeded disc was transferred to a 24-well plate and incubated in ml of the growth medium and maintained at 37ºC in the incubator with subsequent ... group of cell- secreted instructional proteins capable of directing a plethora of cell functions, including migration, mitosis, differentiation, and apoptosis (Chen et al., 2010) The action of these ... sustained release of VEGF for 28 days, resulting in the increase of proliferation of human umbilical vein endothelial cells (King and Patrick, 2000) The microspheres can also be used in combination with...
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Báo cáo hóa học: "Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea" ppt
Ngày tải lên :
18/06/2014, 16:20
... markers as Sox2 and Nestin, and can differentiate in vitro into cells expressing markers of hair cells and supporting cells in vitro[18,31] Culturing organ of Corti progenitor cells under nonadherent ... cells in the inner ear[14,33,38] Nestin-positive cells expanded in culture from proliferating and floating spherical colonies have been shown to incorporate bromodesoyuridine into the DNA indicating ... therapies, including replacement of cells in the inner ear [28] More recent data from mammals suggests that supporting cells or a subset of supporting cells can act as precursors for hair cells, and...
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Báo cáo y học: " Role of pulmonary intravascular macrophages in endotoxin-induced lung inflammation and mortality in a rat model" pot
Ngày tải lên :
12/08/2014, 14:20
... were processed and embedded in paraffin Sections (five micron) were cut onto the polyL-Lysine coated slides and used for hematoxylin and eosin staining and immunostaining Pieces of the left lung ... recruited PIMs in endotoxin-induced mortality in a BDL rats It is striking that depletion of a single inflammatory cell results in remarkable reduction in key inflammatory 11 Competing interests The ... or presence of PIMs PIM reduction protects against LPS-induced mortality in BDL rats We delineated role of PIMs by comparing LPS-induced lung inflammation and mortality in BDL rats and GCtreated...
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Báo cáo sinh học: " Risk factors in the development of stem cell therapy" pdf
Ngày tải lên :
18/06/2014, 19:20
... expansion and culture of stem cells can change the characteristics of the stem cell due to intracellular and extracellular influences Every cell division has a small chance of introducing deleterious ... the intrinsic cellular properties of a particular cell type or class of stem cells (Table 1); secondly extrinsic risk factors introduced by procurement, handling, culturing, or storage of the cells; ... counterparts of embryonic stem cells that originate from the inner cell mass of a blastocyst stage embryo The embryonal carcinoma cells replicate and grow in cell culture conditions In 1981, embryonic stem...
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Báo cáo hóa học: " The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C" ppt
Ngày tải lên :
20/06/2014, 01:20
... for correct folding and intracellular transport of viral glyco- proteins, as shown for instances for influenza hemagglutinin [19] In order to investigate the importance of the individual N-glycosylation ... transported to the cell surface and remain mannose-rich Materials and methods N-glycans play not only an important role in folding and intracellular transport of viral glycoproteins but also are ... of glycosylation sites and the N-glycan trimming differs between recombinant expressed glycoproteins and GP in LASV-infected cells It was shown recently that recombinant expressed M protein of...
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Báo cáo y học: "The role of T cell interleukin-17 in conducting destructive arthritis: lessons from animal models" pps
Ngày tải lên :
09/08/2014, 06:22
... Beer Y: Interleukin-17 enhances tumor necrosis factor alpha-induced synthesis of interleukin 1, 6, and in skin and synovial fibroblasts: a possible role as a ‘fine-tuning cytokine’ in inflammation ... a role for T cells in the initiation and perpetuation of chronic inflammation prevalent in RA Although T cells represent a large proportion of the inflammatory cells during inflammation, T cell- derived ... having an important function in the disease pathogenesis of RA (Fig 1) Role of IL-17 in T cell immunity and/ or propagation of joint inflammation In arthritis, IL-17 is a pro-inflammatory cytokine...
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báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice" pptx
Ngày tải lên :
11/08/2014, 00:23
... Bioadhesivepolysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo Int J Pharmacol 2002, 249:139-147 Neuman RE, Logan MA: The determination of collagen and ... examined in control and treated groups by immunostaining Alb and CK18 proteins increasingly expressed in the MSC/+HGF-CNP treated group compared to other groups (Figure 2A and 2B) despite continuous ... Less invasions of inflammatory cells in the MSC and MSC-+HGF-CNP treated groups connect with the anti-inflammatory action of MSC Moreover, the direct involvement of MSC in immunomodulation of hepatic...
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the role of wasp family members in dictyostelium discoideum cell migration
Ngày tải lên :
22/12/2014, 18:48
... inclusion of profilin and αactinin The role of these proteins in promoting actin nucleation and maintaining actin treadmilling is summarised in figure 1.1c How these factors and many others interact ... depolymerised ADP-bound G-actin is regulated by actin monomer binding proteins Profilins are actin monomer binding proteins that bind G-actin and promote the release of ADP in exchange for ATP (Goldschmidt-Clermont ... F-actin capping, bundling and severing proteins Once nucleated, F-actin interacts with a host of different actin binding proteins, which act to limit elongation, cross-link multiple filaments into...
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the role of fgfr3 mutation in tumour initiation, progression and invasion of urothelial cell carcinoma in mice
Ngày tải lên :
22/12/2014, 20:56
... The role of FGFR3 mutation in tumour initiation, progression and invasion of urothelial cell carcinoma in mice Mona Foth Submitted in fulfilment of the requirements for the Degree of PhD Beatson ... urothelial stem cells within the basal cell compartment using marker expression studies and lineage tracing It has been suggested that 10% of the basal cells could represent candidate stem cells, ... The extracellular domain (ligand binding domain) of each monomer consists of three immunoglobulin (Ig)-like domains (Ig I, Ig II and Ig III) (Wesche et al., 2011) The Ig II and III domains generally...
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Genetic analysis of the role of ARP2 3 complex in border cell migration in drosophila melanogaster
Ngày tải lên :
10/09/2015, 15:48
... prevent the binding of capping factors to the barbed end (Kovar et al 2003) FH1 contains polyproline sequences and interacts with profilin (Chang et al 1997) Since profilin binds to actin monomer, ... domains within Diaphanous besides the FH1 and FH2, including the Diaphanous autoregulatory domain (DAD), DAD interacting domain (DID), and GTPases binding domain (GBD) (Li and Higgs 2005) DAD interacts ... cell clusters formed in mbm germline mutant clone Absence of Mbm staining in germ cells is an indication of mutant clone (C) Western blot test of endogenous Mbm protein in mbm1819 and mbm1, cellular...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
Ngày tải lên :
11/09/2015, 10:14
... Immunoflourescent staining of neural stem cell marker Nestin in SHH-CM and Control-CM treated EB Middle panel shows corresponding DAPI nuclear staining in blue and right panel shows corresponding ... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... the neural tube into the forebrain, midbrain, hindbrain and spinal cord The Shh and bone morphogenetic proteins (BMP) play important roles in patterning the dorsal-ventral aspect of the neural...
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The role of kruppel like factors in embryonic stem cells
Ngày tải lên :
14/09/2015, 14:07
... complex inhibits a complex including Axin, Gsk-3 and APC Inactivation of Gsk-3 results in stabilization and accumulation of β-catenin in the cytoplasm and nucleus β-catenin in collaboration of T cell ... regenerative medicine Stem cells in mammals are mainly classified into four groups according to their differentiation potentials: totipotent stem cells, pluripotent stem cells, multipotent stem cells, and ... trithorax-group inactive X specific transcripts zinc finger protein 42 zinc finger protein 143 zinc finger protein of the cerebellum XI Introduction CHAPTER I INTRODUCTION Introduction CHAPTER I INTRODUCTION...
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The role of TRIP br proteins in the regulation of mammalian gene transcription and cell cycle progression
Ngày tải lên :
16/09/2015, 17:12
... Features PHD zinc finger protein PHD zinc finger protein Composite PHD zinc fingerbromodomain proteins PHD zinc finger protein Bromodomain protein PHD zinc finger proteins PHD zinc finger protein Composite ... Prominent examples of PHD zinc finger and/ or bromodomaincontaining proteins The (*) label designates disease-associated PHD zinc finger and/ or bromodomain-containing proteins Conservation of the ... function of the PHD-bromodomain-interacting domain of TRIP-Br proteins 1.1 Designing peptides that antagonize physical 51 interactions between TRIP-Br proteins and PHD zinc fingers and/ or bromodomain-containing...
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The role of host cell ether lipids in influenza virus infection
Ngày tải lên :
12/10/2015, 17:36
... Briefly, their functions include maintaining the structure of cell membranes, serving as an energy storage, and being involved in membrane trafficking and cellular signalling processes (van der ... observations in influenza virus-infected A549 cells, many choline containing lipids such as phosphatidylcholine (PC) and sphingomyelin (SM) lipid species were significantly different in infected cells, ... protein is involved in the export of vRNP out of the nucleus (Rossman and Lamb, 2011) Influenza M2 proteins are multifunctional proteins, mediating entry, assembly and budding stages of the influenza...
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