rev interaction by affinity chromatography
Báo cáo y học: " Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques" pot
Ngày tải lên :
13/08/2014, 09:20
... tenofovir treatment tion (K64R/K) By week 35, the K64R mutation emerged as the major population in three infected animals Quantification of SIV RNA by real-time PCR revealed the presence of SIVmac055 ... drug treatment augmented by virus-specific immunity can prevent replication of drugresistant virus during chronic SIV infection The animals used in this study were previously found to have robust ... at a dose of 30 mg/kg body weight The effect on SIV replication was determined by quantification of plasma SIV RNA by allele-specific real-time PCR Plasma viral load is expressed as SIV RNA copies/ml...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
Ngày tải lên :
26/10/2012, 09:48
... lower row) by a reversible disulfide-bridge formation, and secondly, the compounds for the ligation in virtue to the Diels-Alder-Reaction with inverse electron-demand extensive described by Braun ... for 144 hours Treatment of lymphocytes was followed by identical procedures according to the DU 145 cells The suspension culture was harvested by centrifugation at 800rpm for 10min, rinsed in PBS ... behaviour could be caused by an arrest of diploid cells in the G1 phase, whereas the cells of the aneuploid fraction pass from the G1 into the S phase This finding is confirmed by the investigation...
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báo cáo hóa học:" Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist" potx
Ngày tải lên :
18/06/2014, 15:20
... 2001, 87(4A):1A-7A Revkin JH, Shear CL, Pouleur HG, Ryder SW, Orloff DG: Biomarkers in the prevention and treatment of atherosclerosis: need, validation, and future Pharmacol Rev 2007, 59(1):40-53 ... temperature for 24 hours followed by processing through the internalization assay Results obtained from the overnight incubation were compared to results obtained by processing the whole blood after ... Alexa-488 labeled MCP-1 (AF488-MCP-1) was purified from the excess labeling reagent and free MCP-1 by RP-HPLC using a Vydac C18 semi-prep column (10 × 250 mm) and BioCad Vision Workstation Labeled...
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báo cáo hóa học:" Static platelet adhesion, flow cytometry and serum TXB2 levels for monitoring platelet inhibiting treatment with ASA and clopidogrel in coronary artery disease: a randomised cross-over study" potx
Ngày tải lên :
18/06/2014, 15:20
... prevent thrombosis in cardiovascular disease The benefits of ASA have been clearly demonstrated by the Anti-platelet Trialists' Collaboration [1] They found that ASA therapy reduces the risk by ... Connecticut, USA) followed by incubation at 4°C at least overnight and for a maximum of days The microplates were then washed two times in 0.9% NaCl by plate inversion followed by the addition of 25 ... followed by statistical complexity reduction is unusual for this type of study Among the variables measuring platelet function, platelet adhesion was represented by eight factors, flow cytometry by...
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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx
Ngày tải lên :
07/08/2014, 20:23
... humans However, the mAb-defined epitope recognized by BAQ44A is expressed on B lymphocytes in multiple species of ruminants The epitope recognized by CADO34A is expressed on granulocytes, B lymphocytes ... Panel shows the LDM recognized by RACT20A is only expressed on basophils, upper right quadrant As noted, Ig is present on basophils Panel shows the LDM recognized by MRB120A is expressed on monocytes, ... cytometry has not revealed any CD4 CD8 double negative subset Likewise comparison of the percentages of lymphocytes expressing LDM on T cells with those expressing LDM on B cells has not revealed the...
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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps
Ngày tải lên :
09/08/2014, 14:22
... performed daily by FACSComp software using CaliBRITE™3 beads Optimal dilution of probes used in FCM was obtained by comparing different dilutions The majority of the unfixed cells were viable by propidium ... FCM analysis of GR expression by western blot Analysis of GR repression in (left) K562 and (right) U937 with and without 10-6 M Dex of GR expression by western blot by GR-mAb FCM and western blot ... been explained One possibility is that cytokines may play a role in steroid resistance by reducing the affinity of GR [19] Therefore the detection of GR utilizing different modalities may be...
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Báo cáo y học: "The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives" pot
Ngày tải lên :
13/08/2014, 13:22
... to another) and the monitoring of their activation by detecting CD63 (which is also expressed to some extent by other activated leukocytes and by activated platelets that may adhere to basophils) ... prostaglandin D2 was by far the most potent activator of basophils, inducing CD203c and CD11b elevation [37] This response was mediated by the DP2 receptor / CRTH2 as it was shared by selective agonists ... agonists of this receptor As previously observed in eosinophils [39], the interaction of prostaglandin D2 with the DP1 receptor limited the activation of basophils by this prostaglandin This suggested...
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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps
Ngày tải lên :
14/08/2014, 17:22
... reassessment of the experimental setup, small variations are adjusted by the model described by equation In particular, they are fitted by the intercept term α The biologically relevant information is ... apoptosis revealed using fluorescence-resonance energy transfer EMBO Rep 2000, 1:266-270 Kolch W: Meaningful relationships: the regulation of the Ras/ Raf/MEK/ERK pathway by protein interactions ... healthy cells is approximated by Correlation of fluorescence and cell size Regardless of the presence of fluorochromes, every cell emits light when it is excited by a laser - a phenomenon referred...
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Intake manifold design effect on air fuel mixing and flow for an LPG heavy duty engine
Ngày tải lên :
05/09/2013, 16:30
... engine Go- Kart engine The study is done by three dimensional simulations of the flow Simulations are validated by an experimental study From this study, they reveal that the variations in the geometry ... beginning, the 3D geometries construct of intake manifolds are built by the CAD software SW; as shown in Figure The files created by SW are imported into FloWorks to build mesh for final simulation ... heat flux The mixture density is calculated by [20]: m propane ρ = mair × ρ propane m propane mair + ρ air (5) +1 The turbulent viscosity is given by: µ t = Cµ ρ k2 ε (6) This viscosity is a...
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Clinical Flow Cytometry – Emerging Applications Edited by Ingrid Schmid ppt
Ngày tải lên :
08/03/2014, 19:20
... was purified and characterized by high resolution chromatography (HPLC) and mass spectrometry (MS-MS), supplemented by the methods of Edman (Edman & Begg, 1967) This revealed a pentapeptide with ... IL-2 by rapid proliferation Because IL-2 is secreted by effector T cells, this provides a negative-feedback mechanism, in which inflammatory T-cell activity (e.g., by Th1 cells) is restrained by ... detected by FCM The multiparameter analysis permitted by FCM can be used to study other characteristics, such as parasite antigens expressed by the erythrocyte (which can be detected by antibodies...
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flow cytometry protocols 2d ed - teresa s. hawley
Ngày tải lên :
08/04/2014, 12:51
... available when cells differ from each other by the way they scatter light, by slight differences in antigen intensity, or by multiparameter criteria Cells sorted by flow cytometry are routinely used ... be determined by the number of cells flowing per second, by the vibration frequency that is creating the drops, and by the impact of the mathematics of a Poisson distribution, whereby cells are ... of the detectors will measure light excited by the first laser, some will measure light excited by the second laser, and so forth The signals emitted by a cell as it passes through each laser beam...
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flow cytometry protocols
Ngày tải lên :
11/04/2014, 09:41
... utilized have been reviewed by Lewis (I) The intracellular detection of cytokine protein by flow cytometry, which can be used in conjunction with surface-marker analysis (by using two or more ... measurements in AML cells by flow cytometry have come from work with the Fix & Perm reagents produced by An Der Grub Bio Research (Austria) and distributed in the United Statesby Caltag (San Francisco, ... appropriately Blast cells are identified by the simultaneous expression of FITC-labeled TdT and the PE-labeled surface antigen of choice prevrously shown to be expressed by the majority of blast cells...
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hughes t. groove and flow - six analytical essays on the music of stevie wonder (диссертация)
Ngày tải lên :
29/05/2014, 21:49
... played by Wonder’s brother Calvin Hardaway, the judge by his lawyer Johanan Vigoda, the criminal by his road manager Ira Tucker, Jr., and the jailor who says “Get in that cell, nigger!” by a white ... generates one type of flow by periodically adding heavy reverb to the bass drum only on the second beat of a bar.21 There are qualitative differences between the flow generated by different musical ... copy of a doctoral dissertation by Timothy S Hughes and have found that it is complete and satisfactory in all respects, and that any and all revisions required by the final examining committee...
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Báo cáo hóa học: "Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments" pot
Ngày tải lên :
18/06/2014, 16:20
... following the LTM leukopak collection The protocol was reviewed by NCI’s CTEP and approved by the Providence Health System institutional review board All patients gave written informed consent ... generated by different panels of variable markers Additionally our approach recognizes that potential sets of interest are both those defined by all variable markers, and those defined by subsets ... All protocols were approved by the IACUC of Legacy Research and Technology Center A bilateral compartment syndrome injury was produced in the anterior tibialis muscles by infusing porcine plasma...
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CLINICAL FLOW CYTOMETRY – EMERGING APPLICATIONS pot
Ngày tải lên :
28/06/2014, 15:20
... was purified and characterized by high resolution chromatography (HPLC) and mass spectrometry (MS-MS), supplemented by the methods of Edman (Edman & Begg, 1967) This revealed a pentapeptide with ... IL-2 by rapid proliferation Because IL-2 is secreted by effector T cells, this provides a negative-feedback mechanism, in which inflammatory T-cell activity (e.g., by Th1 cells) is restrained by ... detected by FCM The multiparameter analysis permitted by FCM can be used to study other characteristics, such as parasite antigens expressed by the erythrocyte (which can be detected by antibodies...
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Báo cáo y học: " Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assays" potx
Ngày tải lên :
09/08/2014, 08:22
... determination within the context of a diagnostic investigation Patients were diagnosed by a clinician by reviewing of files and the patients were classified in accordance with the ACR classification ... anti-pepB antibodies by line immunoassay Anti-pepA and anti-pepB Abs were detected by a research line immunoassay containing two citrulline-containing peptides, as described previously (INNO-LIA™ ... evaluated, as proposed by Hanley [21] Agreement between dichotomized variables was measured by the (weighted) κ statistic Proportions of matched-pair data were compared by means of the McNemar...
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Báo cáo khoa học: "Cutaneous vascular reactivity and flow motion response to vasopressin in advanced vasodilatory shock and severe postoperative multiple organ dysfunction syndrome" pot
Ngày tải lên :
12/08/2014, 23:22
... dysfunction syndrome (Table 1) Materials and methods The study protocol was approved by the institutional review board and the ethical committee of the Innsbruck Medical University, Innsbruck, ... (perfusion units = 250) was derived by measurement in a motility standard fluid (Perimed) The electrode was placed on the volar aspect of the forearm and held in place by a thin transparent silicon ... the response of the oscillation frequency detected by laser Doppler flowmetry Results Cardiovascular function could not be stabilized adequately by incremental dosages of norepinephrine in one patient...
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Báo cáo y học: "Differential response of human basophil activation markers: a multi-parameter flow cytometry approach" pot
Ngày tải lên :
13/08/2014, 13:22
... with CD203c triggered by fMLP or by anti-IgE and with CD13 triggered with fMLP CD63-FITC MFI, very low in resting cells (mean 619.76), increased by 24 times following fMLP and by 4.8 times following ... test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives Clin Mol Allergy 2005, 3:9 Triggiani M, Marone G: Basophil's secrets revealed by flow ... automatically with the cytometer software by averaging the total fluorescence of the marker in the basophil gate As well percentage of activated cells was calculated by the software considering the CD63-FITC...
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Influence of pipe length and flow rate on nano particle deposition in laminar circular pipe flows
Ngày tải lên :
09/09/2015, 10:06
... for validating the Brownian motion The first employed equation was developed by Thomas [2] at 1976 which is calculated by the following equation: DE = − (0.819e −14.63∆ + 0.097 e −89.2 ∆ + 0.032e ... is developed by Ingham at 1975 which is defined as follow [3]: DE = − (0.819e −14.63∆ + 0.0976e −89.22∆ + 0.0325e − 228∆ + 0.0509e −125.9∆ 2/3 ) (10) The third one is developed by Yeh and Schum ... contour at the midsection of the pipe Deposition results for the Brownian motion models are verified by comparing the results with different analytical expressions for the fully developed flow in pipes...
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