results and how would you manage the neonate
Báo cáo y học: "Modeling the clonal heterogeneity of stem cells" doc
Ngày tải lên :
13/08/2014, 16:20
... on the various clones For clarity, we shall only display the results of the one and the two species cases (one and two dimensions) In Section 5.3, we illustrate the steps necessary to extend the ... sampling of values of f11 (the efficacy of the first treatment for the first clone, on the x axis The results for various values of f22, the efficacy of the second treatment for the second clone are ... p1 and p We specify the amount of disturbance that will allow both clones to sur- vive given the growth and death rates and the interaction parameters (the b’s) Suppose ∞ ∞ q1 = q , where the...
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Báo cáo y học: "Deciphering the molecular machinery of stem cells: a look at the neoblast gene expression profile" pps
Ngày tải lên :
14/08/2014, 20:22
... [7] These cells are scattered throughout the parenchyma with the exception of the anterior end of the cephalic region and the pharynx, and accumulate preferentially in the dorsal region, along the ... μm, they are the smallest cells in the planarian body and are the principal cell type expected to be found after the filtering procedure through a progressive series of meshes (50 μm, 20 μm, and ... expressed in the dispersed neoblasts and only slightly detectable at the level of the clustered neoblasts (Figure 7g) reports In all cases the results obtained were consistent with the transcriptional...
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Microgel iron oxide nanoparticles for tracking of stem cells through magnetic resonance imaging
Ngày tải lên :
14/09/2015, 08:49
... the CNS There are two parts to the mammalian immune response to pathogens such as bacteria and viruses The first is the innate immune response which is the immediate and generic response of the ... have the ability to home and migrate to sites of tissue injury, where they may participate in therapeutic activities However, transplanted stem cells may also end up in other parts of the body Therefore, ... adhesion steps is initiated by surface tethering, which are mainly mediated by P and E selectins and their ligands Following tethering, the captured cells roll and encounter chemokines, which eventually...
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In vitro differentiation of stem cells towards islet like cells
Ngày tải lên :
09/10/2015, 11:18
... its simplicity and convenience However, the work on safety and the efficiency of the therapeutic gene carrying vector is still in progress There are few strategies on in vivo gene therapy, which ... (Blyszczuk, Asbrand et al 2004) They managed to induce the differentiation of unselected ES cells into islet-like clusters that express Pdx1, Pax4, and insulin The islet-like clusters showed the glucose ... transplantation, the immunosuppressive therapy following islet transplantation also has significant adverse effects Therefore, there is still the need for further progress in the availability...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... culture, the medium was refreshed, and then the medium changed every other day Cell passaging was performed when cell confluence reached 85% The purity and immunophenotype of MSCs and their potentials ... smooth and round, and the articular cartilage was transparent and glossy (Figure 5A) After surgery, the shape of femoral head was not markedly changed and the surface of femoral head was pale and ... head The normal femoral head was smooth and round, and the articular cartilage was transparent and glossy (A) After freezing, the femoral head was pale in the absence of normal glossiness and...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... emphasized the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the H9 line isolated by Thomson and ... proper pH, humidity and CO2 balance For the 4oC and 25oC exposure groups, the 6-well culture plates were sealed at the sides with parafilm (for minimal evaporation), and placed in either a 4oC refrigerator, ... temperature, the proportion of Grade A, B and C colonies were 94.5%, 3.0% and 2.5% respectively for 24h exposure to 4oC (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
Ngày tải lên :
18/02/2014, 04:20
... increases the transcription but not the secretion of IL-1b and TNF-a, suggesting that the roles of these factors in the paracrine effects of MSCs are negligible However, hypoxia ⁄ SD also enhances the ... (data not shown) and enhanced IL-10 expression Therefore, MG132 preconditioning may provide another effective strategy of maximizing the viability, paracrine effects and biological and functional ... degradation of IjB The active form of NF-jBp65 is then released and translocated into the nucleus to activate the transcription of IL-1b and TNF-a In this report, hypoxia ⁄ SD-induced IL-1b and TNF-a...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
Ngày tải lên :
07/03/2014, 12:20
... been made at other loci, such as at the lysozyme locus [16], c-fms [17], and the myeloperoxidase gene [18] Understanding how epigenetic states are acquired during development and how they impact ... least two independent experiments The results show that RNA Pol II, TPB, and Me2K4H3 are present at the core regions of the LCR (HS2 and HS3) but not at the ec- and bmajor-globin genes in undifferentiated ... HS2 (3¢HS2), and the bmaj-globin gene (bmaj) The panel on the right shows that transcription 5¢-to HS3 is directional and proceeds towards the HS3 core enhancer The RNA was isolated and reverse...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
Ngày tải lên :
15/03/2014, 09:20
... science and technology and to their use for the general welfare Upon the authority of the charter granted to it by the Congress in 1863, the Academy has a mandate that requires it to advise the federal ... or the brain, in the adult body They can renew themselves in the body, making identical copies of themselves for the lifetime of the organism, or become specialized to yield the cell types of the ... all the members of the committee for their valuable contributions and especially for their insights into both the scientific and the societal issues In particular, Corey Goodman, chair of the...
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Atlas of Human Pluripotent Stem Cells doc
Ngày tải lên :
22/03/2014, 09:20
... and Knowles during the 1970s for separating between the trophoectoderm layer and the ICM (Solter and Knowles 1975) This achieved, and following removal of the zona pellucida (ZP) (Fig 1.2), the ... facilitate the realization of the enormous potential of human pluripotent stem cells and shorten the path from the bench to the bedside We are grateful for the generous support of the Technion’s ... Technion’s Stem Cell Center by the Sohnis and Forman Families We thank Ilana Laevsky and Atara Novak for their valuable contributions The cooperation and enthusiasm shown by the staff members at Springer...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
Ngày tải lên :
23/03/2014, 07:20
... cells from the early blastomere stage to the blastocyst stage is accompanied by a change in the epigenetic profile that directs the differentiation pathway to either the embryo proper or the placenta ... methylation However, following these studies, it was still unclear how Dnmt1 and Dnmt3a ⁄ 3b are involved in DNA methylation in T-DMRs, thereby establishing DNA methylation profiles of cells, and whether ... methylation profiles during the developmental process To examine how the DNA methylation profile changes as the embryo develops, we utilized model differentiation systems and analyzed the DNA methylation...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
Ngày tải lên :
30/03/2014, 04:20
... by the dendrogram above to show the similarity of their gene expression patterns The expression profile of each gene is represented in the respective rows Genes are linked by the dendrogram on the ... 0.0064 a Of the genes analyzed, the GO biological process is known for 739 up-regulated genes and 215 down-regulated genes Others are unknown b Of the genes in the mother population, the GO biological ... for 12 441 Others are unknown c The significance of the appearance of the GO term (biological process) in the up-regulated and down-regulated genes was calculated as a P value by the software...
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differentiation of embryonic stem cells
Ngày tải lên :
11/04/2014, 00:32
... VOLUME 85 Structural and Contractile Proteins (Part B: The Contractile Apparatus and the Cytoskeleton) Edited by DIXIE W FREDERIKSEN AND LEON W CUNNINGHAM VOLUME 86 Prostaglandins and Arachidonate ... Biology and Genetics (Part A) Edited by CHANDAN K SEN AND LESTER PACKER VOLUME 353 Redox Cell Biology and Genetics (Part B) Edited by CHANDAN K SEN AND LESTER PACKER VOLUME 354 Enzyme Kinetics and ... cells representative of the primary germ layers, ectoderm, endoderm, and mesoderm Further differentiation results in formation of many of the cell populations of the embryo and adult including beating...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
Ngày tải lên :
18/06/2014, 15:20
... CD31 and eNOS [10] Therefore, we used the term 'eEC' for these ECs in the early differentiation stage We sorted and expanded these eECs in vitro These eECs were cultured in the presence of VEGF and ... over the course of 50 seconds and checked the time until the mouse fell off The exercise time (seconds) on the rota-rod for each mouse was recorded just before the experiments (= day 0) and on ... MCAo However, the blood flow of the ipsilateral side in the hES-EC+MCinjected group, especially pointed out by the arrow, clearly increased up to or rather than the corresponding area in the contralateral...
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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc
Ngày tải lên :
18/06/2014, 15:20
... factor in the nerve growth factor family of neurotrophins helps support the survival, growth and differentiation of both existing and new neurons and synapses in vivo and ex vivo In the study the supplement ... in part by the grants from the 7042014 of the National Science Foundation of Beijing, China, the 50573044 of the National Natural Science Foundation of China and the 2005CB623905 of the National ... References The authors declare that they have no competing interests Authors' contributions HZ and TWY collected and analyzed data KST interpreted data and wrote the manuscript CRS, JL and HH acquired...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... (B) Figure Sequence and GO analysis of the miR-302 cluster and miR-520 cluster Sequence and GO analysis of the miR-302 cluster and miR-520 cluster The members of the miR-302 and miR-520 clusters ... miR-373 induces the expression of E-cadherin and CSDC2 by targeting their promoter region and initiate their expression[73] Another mechanism is that the engagement of miRNA and their targets at ... collected and analyzed the data and wrote the manuscript PJ carried out data analyses and assisted with writing the manuscript EW designed the miRNA-array and oligo platform, developed protocol and...
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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx
Ngày tải lên :
18/06/2014, 15:20
... isolated and showed the ability to differentiate into cell types of the three germ layers [17-23] The human fallopian tubes (hFTs) share the same embryologic origin as the uterus They have the capacity ... CD44 and CD90) and other MSC markers (CD13, CD73, SH2, SH3 and SH4) together with the multilineage differentiation results confirmed the mesenchymal nature of human fallopian tube stem cells These ... muscle control and in the induced hFTs cells 1B) Skeletal myosin expression in muscle control and in the induced hFTs cells 1C) Myosin band observed in the muscle control and in the induced hFTs...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
Ngày tải lên :
18/06/2014, 16:20
... interpreted data and conceptualized manuscript RH, JAK, JK, KWA, CAS, BM, ANP, MPM, LS, FR, and TEI provided detailed ideas and discussions, and/ or writing of the manuscript NAM and JAJ performed the experiments ... Markin, The Aidan Foundation, and the Center For The Improvement Of Human Functioning International Author details Bio-Communications Research Institute, Wichita, Kansas, USA 2The Center For The ... stimulated and not stimulated cells was calculated and compared for different periods before and after intervention Stem-Kine mobilizes CD34 and CD133 Cells Statistics Differences between the groups...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
Ngày tải lên :
18/06/2014, 16:20
... safety and efficacy of the product There is still much to be learned about the procedures to establish the safety and efficacy of cell therapy products The greater the understanding of the biology ... biocompatible and, as synthetic polymers, may be incorporated active biomolecules They have however the disadvantages that they may mimic the natural structure and composition of extracellular matrix, their ... in 2006 with the support of the Ellison Medical Foundation, The Greenwall Foundation, and Howard Hughes Medical Institute These updates and amendments have updated the guidelines of the different...
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