regulation of glycosyltransferase genes in apoptotic breast cancer cells induced by l ppmp and cisplatin
Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf
Ngày tải lên :
17/03/2014, 23:20
... human breast cancer cell line L5 6Br-C1 This cell line carries a germ-line mutation (1806 C fi T) in the BRCA1 tumour Fig Propidium iodide-stained nuclei of L5 6Br-C1 cells Cells were seeded in the absence ... as in any of those two cell lines The depletion of the polyamine pools was however, similar in all three cell lines The differences in response to DENSPM treatment are presumably reflected in ... observed in SK-MEL-28 cells [14], a human melanoma cell line that contains a mutated p53 gene In the present work, DENSPM was also found to rapidly and extensively induce cell death in the human breast...
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Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors potx
Ngày tải lên :
22/03/2014, 17:20
... FBS (vol/ vol), 1% penicillin streptomycin (vol/vol), and μg/mL ciprofloxacin MCF7/Her2-18 and parental CHO cells were cultured in an incubator at 37°C with 5% CO2 and maintained at a cell concentration ... concentration of between × 105 and × 10 viable cells/ mL MDA-MB-231 cells were cultured in an incubator at 37°C with no CO2 and maintained at a cell concentration of between × 105 and × 106 viable cells/ mL ... receptor levels on several cell lines reported to express varying levels of Her2, including breast cancer cell lines MCF7/Her2-18 Hathaway et al Breast Cancer Research 2011, 13:R108 http:/ /breast- cancer- research.com/content/13/5/R108...
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Báo cáo khoa học: Inhibition of human MDA-MB-231 breast cancer cell invasion by matrix metalloproteinase 3 involves degradation of plasminogen docx
Ngày tải lên :
31/03/2014, 09:20
... with intact plasminogen (10 lgÆmL)1) in the presence of BSA (10 lgÆmL)1) resulted in cell–plasminogen association, as determined by casein zymogram of washed cell lysates Cells incubated with plasminogen, ... CaCl2, 100 lgÆmL)1 leupeptin, 2.5 mgÆmL)1 pepstatin A and mM phenyl methyl sulfonylflouride (pH 8.0) Cells were disrupted by cycles of freezing and thawing in liquid nitrogen and at 42 °C Nuclei ... mean of 410 cells per filter invaded in the presence of plasminogen Exogenous recombinant pro-MMP-3 (0.1 and lgÆmL)1) inhibited MDA-MB-231 invasion in the presence of plasminogen by 24% and 49%...
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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf
Ngày tải lên :
09/08/2014, 09:20
... carcinoma cells and Lewis Lung Carcinoma (LLC) cells [4,8] We also showed that DCQ inhibited cell proliferation and induced apoptosis in colon T-84 cancer cell lines under normoxia via the inhibition ... and DSB induced by DCQ in p53+/+ cell line (A) Examples of comets induced by DCQ in cells subjected to the alkaline comet assay Cells treated with DCQ for hrs in normoxia or hypoxia were collected ... percentage of Annexin-positive apoptotic cells (Figure 3C) In Figure 3C, quadrant A represents apoptotic cells, B apoptotic and necrotic cells, C normal cells and D necrotic cells The percentage of apoptotic...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
Ngày tải lên :
12/08/2014, 02:20
... proliferation and differentiation of B cells, and proliferation of T cells As a result, all these TNF-a activated mediators could contribute to the infiltration of inflammatory cells into the influenza infected ... CO2 incubator Mandin-Darby canine kidney (MDCK) cells were used for growing stocks of influenza virus isolates MDCK cells were grown and maintained in Eagles Minimal Essential Media (MEM) containing ... bronchial epithelial cells induced by influenza virus A Int Arch Allergy Immunol 1997, 113:307-311 39 Hao S, Baltimore D: The stability of mRNA influences the temporal order of the induction of genes...
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Báo cáo y học: " Identification of fusion genes in breast cancer by paired-end RNA-sequencing" potx
Ngày tải lên :
09/08/2014, 22:23
... serum, 1.5 mM L- glutamine and penicillin/streptomycin All cells were cultured at 37°C under 5% CO2 Sequencing library construction and paired-end RNA- Total RNA from breast cancer cell lines (see ... VAPB and CYTH1 (middle) and RPS6KB1 and SNF8 (right) in BT-474 cells but not in control cells White arrows indicate fused genes Coloring of the gene names coincides with labeling of the BAC clones ... Sigrid Juselius Foundation and the Cancer Society of Finland Author details Institute for Molecular Medicine Finland (FIMM), Tukholmankatu 8, Helsinki, 00290, Finland 2Medical Biotechnology, VTT...
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báo cáo khoa học: "Aberrant DNA methylation of cancer-related genes in giant breast fibroadenoma: a case report" docx
Ngày tải lên :
10/08/2014, 23:20
... analysis of the methylation profile of 26 cancer related regions in invasive breast carcinomas by MS-MLPA and drMS-MLPA Mol Cell Probes 2010, 24:271-280 Di Vinci A, Perdelli L, Banelli B, Salvi ... was provided by SECTyP, National University of Cuyo (06-J343) and the School of Medical Sciences, National University of Cuyo, Mendoza, Argentina Author details Cellular and Molecular Laboratory, ... epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications BMC Cancer 2010, 10:297 12 Ottaviano YL, Issa JP, Parl FF, Smith HS, Baylin SB,...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
Ngày tải lên :
07/03/2014, 05:20
... examine the kinetics of the apoptotic cell death induced by ionizing radiation in human cervical cancer cells, we treated HeLa cells with 10 Gy radiation, and analyzed induction of apoptotic cell ... role in radiation -induced apoptotic cell death in human cervical cancer cells Inhibition of JNK effectively protected cells from radiation -induced loss of mitochondrial membrane potential and apoptotic ... medium supplemented with 10% fetal bovine serum All media were supplemented with 100 unitsÆmL)1 penicillin and 100 lgÆmL)1 streptomycin, and all cells were incubated at 37 °C in 5% CO2 Cells were...
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Báo cáo hóa học: " Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early-stage breast cancer" pot
Ngày tải lên :
18/06/2014, 16:20
... mathematical modeling and statistical analyses, LM - performed experiments, interpreted results, KB - collected and interpreted clinical data, EN - collected and interpreted clinical data, MS ... published aiming to verify the applicability of MALDI- and SELDI-based analyses of the low-molecular-weight fraction of blood proteome for cancer diagnostics Although no single peptide could ... outliers by using Dixon test based on areas of the raw spectra, averaging of technical repeats, binning of neighboring points to reduce data complexity, removal of the spectral area below baseline and...
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báo cáo hóa học:" Heath-related quality of life in Spanish breast cancer patients: a systematic review" pptx
Ngày tải lên :
20/06/2014, 15:20
... Neoplasm” AND “Spain” in PubMed, EMBASE and PsycINFO, with Breast Cancer also being used as a key word; “Quality of life” AND Breast cancer in Dialnet; “quality” AND “life” AND cancer AND breast ... breast in IBECS; “quality of life” and breast cancer and “Spain” as the key words in CUIDEN; cancer AND breast AND “quality” AND “life” in ISOC; and lastly, the terms “Neoplasms of breast , ... Craven-Bartle J, Sierra J, Arcusa A: A randomized trial of the effect of training in relaxation and guided imagery techniques in improving psychological and quality -of- life indices for gynecologic and breast...
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báo cáo hóa học:" The effect of peer-led education on the life quality of mastectomy patients referred to breast cancer-clinics in Shiraz, Iran 2009" pot
Ngày tải lên :
20/06/2014, 16:20
... our findings will contribute to delegating caring responsibility to these patients and facilitating the establishment of a counseling center in the breast cancer clinics Overall breast cancer ... Cancer clinic in Shiraz (Dr Talei) and director of Chemotherapy center in Nemazee hospital (Dr Mohamadi) and all the personnel for their valuable help and cooperation Also the authors would like ... the life quality of 99 breast cancer patients using standard life quality assessment tools The results of the current study indicated an increase in the life quality related to breast cancer before...
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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
Ngày tải lên :
09/08/2014, 07:21
... of cell-cell adhesion molecules E-cadherin, α-, β- and γ-catenin in human breast cancer by quantitative real time polymerase chain reaction The mRNA levels of these markers were related to clinicopathological ... Following extensive washings, sections were incubated for 30 in the secondary biotinylated antibody (Multilink Swine anti-goat/ mouse/rabbit immunoglobulin; Dako Inc.) Following washings, Avidin ... or abnormal location of E-cadherin has been observed [3-8] Studies on both cell lines and clinical materials have provided evidence for the involvement of E-cadherin in suppressing cancer progression[5,9-13]...
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Báo cáo khoa học: "Is standard breast-conserving therapy (BCT) in elderly breast cancer patients justified? A prospective measurement of acute toxicity according CTC-classification" pot
Ngày tải lên :
09/08/2014, 09:20
... helping to define the Page of study endpoints KE was also actively involved in the data collection BK helped to define the principle concept of the study as a long-term institutional goal BK also ... according axilla and arm scales were extremely low In fact, axillar pain did improve from initial level of 39.8% grade and Galalae et al Radiation Oncology 2010, 5:103 http://www.ro-journal.com/content/5/1/103 ... documented according “hair loss”, “sweat gland function”, and “pain” Toxicity levels in the scales “hair loss” and “sweat gland function” were clinically insignificant Grade (mean grades close to 0)...
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Báo cáo khoa học: "Tangential beam IMRT versus tangential beam 3D-CRT of the chest wall in postmastectomy breast cancer patients: A dosimetric comparison" docx
Ngày tải lên :
09/08/2014, 09:20
... 76(3):300-310 35 Harsolia A, Kestin L, Grills I, Wallace M, Jolly S, Jones C, Lala M, Martinez A, Schell S, Vicini FA: Intensity-modulated radiotherapy results in significant decrease in clinical toxicities ... including the internal mammary chain in left-sided breast cancer: dosimetric evaluation Radiother Oncol 2009, 90(1):99-105 30 Cozzi L, Fogliata A, Nicolini G, Bernier J: Clinical experience in ... patients in a clinical trial of post-operative radiotherapy Clin Radiol 1989, 40(2):204-208 Paszat LF, Mackillop WJ, Groome PA, Schulze K, Holowaty E: Mortality from myocardial infarction following...
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Báo cáo khoa học: "The prognostic value of the nodal ratio in N1 breast cancer" pps
Ngày tải lên :
09/08/2014, 09:21
... Elliott EA, Parda DS, Pierce LJ, Shepherd L, Vallow LA, White JR, Whelan TJ: A clinical trial of breast radiation therapy versus breast plus regional radiation therapy in early-stage breast cancer: ... Trialists’ Collaborative Group (BCTCG) showed the overall absolute reduction of 5-year locoregional recurrence by 19%, resulting in a 5% overall absolute reduction of 15-year breast cancer mortality ... management of clinical data SWK, JHK, YJK, JSK, and IAK provided clinical expertise in clinical breast oncology SYP contributed to the pathologic work All authors read and approved the content of manuscript...
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báo cáo khoa học: "Evaluation of HER2 and p53 expression in predicting response to docetaxel-based first-line chemotherapy in advanced breast cancer" docx
Ngày tải lên :
10/08/2014, 10:21
... cyclindependent kinase inhibitor [37-39] Therefore, the subcellular localization of docetaxel molecular target and the timing of docetaxel action during cell cycle not overlap with those of p53 ... staining in fewer than 10% of the tumor cells (0); weak staining in part of the membrane in more than 10% of the tumor cells (1+); complete staining of the membrane with weak or moderate intensity ... occurs in a late phase of cell cycle (G2/M transition) Conversely, p53 mainly (but not exclusively) acts in early phases of cell cycle inducing, after DNA damage, a G1 arrest by transactivation of...
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báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
Ngày tải lên :
10/08/2014, 10:21
... protein expression of estrogen recepter alpha(ER a ), matrix metalloproteinase 9(MMP-9), cyclinD1, and on cancer cells invasion, proliferation and cell cycle cell in two cell lines were investigated ... MDA-MB-231 and MCF-7 cells were plated in six-well plates at a density of × 105 cells per well and incubated overnight Cells were transfected with pG, pGM1, pGM2 and blank control, using Lipofectamine ... human breast cancer cell lines MDA-MB231, and protein levels of MMP-9 and CyclinD1 were down -regulation (P < 0.05) However, in ER alpha-positive breast cancer cells MCF-7, protein expression levels...
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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx
Ngày tải lên :
10/08/2014, 10:21
... epithelial cells [35] To distinguish between different populations of cells, double labeling of cellular cytokeratin and vimentin was performed Cells were seeded on Matrigel ® -coated cover slides ... F344 and Lewis cells Salivary a-amylase was similarly expressed in cultured rat mammary epithelial cells from F344 and Lewis, showing its localization in the cytoplasm (Figure 1c,d) In F344 cells, ... Page of 12 b) Lewis cells (P0) 100 μm 100 μm d) Lewis cells (P1) c) F344 cells (P1) 10 μm 10 μm Figure Differences in cultures of primary mammary cells from F344 and Lewis rats and cellular localization...
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Báo cáo y học: " Breast conserving surgery with preservation of the nipple-areola complex as a feasible and safe approach in male breast cancer: a case report" pptx
Ngày tải lên :
11/08/2014, 23:21
... Journal of Medical Case Reports 2008, 2:126 Clinically, he had a lump centrally in the left breast, at a o'clock position, with skin tethering and mild inversion of the nipple Ultrasound (US) and ... Role of adjuvant chemotherapy in male breast cancer Cancer 1989, 64:1583-1585 Authors' contributions SL and GF collected the data and reviewed the literature and case notes and was involved in ... oncologists and patient about the risk of recurrence, we proceeded with BCS with nipple preservation and SNB The sentinel node was involved and level III axillary clearance was performed Overall,...
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báo cáo khoa học:" The effect of peer-led education on the life quality of mastectomy patients referred to breast cancer-clinics in Shiraz, Iran 2009" pot
Ngày tải lên :
12/08/2014, 01:21
... our findings will contribute to delegating caring responsibility to these patients and facilitating the establishment of a counseling center in the breast cancer clinics Overall breast cancer ... Cancer clinic in Shiraz (Dr Talei) and director of Chemotherapy center in Nemazee hospital (Dr Mohamadi) and all the personnel for their valuable help and cooperation Also the authors would like ... the life quality of 99 breast cancer patients using standard life quality assessment tools The results of the current study indicated an increase in the life quality related to breast cancer before...
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