rda analysis of up regulated transcripts showed that glutathione s transferase mrna levels increased in mgh cells after 2 hours of lyo bcg treatment

Mycobacterium bovis, BCG modulation of gene expression in bladder cancer

Ngày tải lên : 11/09/2015, 10:06

... 4 .2. 3 RDA analysis of up- regulated transcripts showed that Glutathione- S- Transferase mRNA levels increased in MGH cells after hours of Lyo BCG treatment …… 94 4 .2. 4 Comparisons of gene expression ... 49 2. 2.5 GSTT2 silencing and its effects on Lyo BCG treatment of MGH cells ………… 53 2. 2.5.1 GSTT2 siRNA transfection and lyo BCG treatment …………………………… 53 2. 2.5 .2 Real time validation of GSTT2 silencing………………………………………… ... reduction in BCG blocked samples…………………………… 103 4.3 Effects of GSTT2 silencing on lyo BCG treatment of MGH cells ……………… 107 4.3.1 Transfection with GSTT2 SMARTpool siRNA successfully reduces GSTT2 expression……………………………………………………….…………………...

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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

Ngày tải lên : 21/02/2014, 01:21

... FEBS 20 02 Omega-class GST from Schistosoma mansoni (Eur J Biochem 26 9) 5513 At least ﬁve GST activities have been described in the human parasite Schistosoma mansoni (SmGST-1 to SmGST-5) SmGST-5 ... Construction of a homology model of SmGSTO SmGSTO structure was built using the human GSTO 1–1 structure as template in the SWISS-MODEL modeling RESULTS SmGSTO DNA and protein sequence BLAST search of ... His-tagged SmGSTO (B) Batch analysis of His-tagged SmGSTO to GSH-agarose Lane 1, protein used in the binding step Lane 2, supernatant of the binding-step Lane 3, GSH eluted fraction (C) Batch analysis...

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Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx

Ngày tải lên : 16/03/2014, 11:20

... demonstrating successful inhibition of de novo synthesis of pathologic PrPSc in persistently prion-infected ScN2a cells In contrast, a strong band at the expected size of PrPSc was observed in cells ... exclusion of dead cells by 7-aminoactinomycin D staining, analysis was performed using a FACScalibur ﬂow cytometer (Becton Dickinson, Franklin Lakes, NJ) Results are expressed in ﬂuorescence units ... mAb 6H4 has been well established as one of the most widely used Abs in prion diseases, and is employed in routine diagnostic tests for transmissible FEBS Journal 27 4 (20 07) 1747–1758 ª 20 07 The...

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Báo cáo khoa học: Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx

Ngày tải lên : 23/03/2014, 09:21

... at least six classes of cytosolic GSTs in insects [2] The majority of GSTs are in the Delta and Epsilon classes, and the remaining enzymes are in the Omega, Sigma, Theta and Zeta classes The ... in Delta class GSTs of other insect species [17 ,22 ,23 ,25 – 27 ], indicating that the unusually high catalytic rate is not a reﬂection of the binding of substrate and cosubstrate Previously reported ... DmGSTD1 NM_0796 02 NlGST1-1 LcGST1 AgGSTD3 BmGST1 MsGSTolf1 AgGSTD7 AgGSTS1 Bla g DmGSTS1 AF448500 L23 126 AF273039 AB176691 AF13 326 8 AF071161 AF513639 U 924 12 NM_16 621 7 MsGST2 AgGSTE2 AgGSTE1 AgGSTO1...

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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf

Ngày tải lên : 30/03/2014, 13:20

... plates Wells containing single cells were marked, and growth in these wells was monitored daily Expression of hGSTA4-4 protein was ascertained by Western blot analysis Site-directed mutagenesis of ... surprising but it seems to be consistent with numerous previous studies suggesting that 4-HNE is involved in cell-cycle signaling mechanisms Our results show that transfection or microinjection of ... being linked to other possible effects of hGSTA4-4, such as a hypothetical direct binding to signaling kinases, as has been described for Pi-class GSTs [30] To test further the hypothesis that...

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Báo cáo khoa học: Puriﬁcation and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx

Ngày tải lên : 31/03/2014, 09:20

... characteristics of R decussatus GST isoforms Results are expressed as means ± SD Reverse phase HPLC retention time (Rt) of subunits expressed in minutes; apparent molecular mass of each subunit expressed ... al [28 ] Species GST 44 GSH binding protein GST GST Consensus mu Consensus alpha Consensus pi N-terminal sequence Identity (%) Ruditapes decussatus Mytilus edulis Clonorchis sinensi Fasciola hepatica ... antisera speciﬁc of mammalian pi-class GST Studies of aquatic species such as salmonids [26 ], Bufo bufo [27 ] and M edulis [28 ], showed that their GSTs belong to the pi class The GST isoforms 1-1...

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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green ﬂuorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc

Ngày tải lên : 31/03/2014, 15:20

... 26 9) 25 79 Table Antisense ODN sequences Target siteb Name Sequencea Regionb GSTM1 GSTM2 Mismatch c (number of bases) AS-ctrl AS-GFP AS-1 AS -2 AS-3 AS-4 AS-5 AS-6 AS-7 AS-8 AS-9 AS-10 AS-11 AS- 12 ... cells With this assay, we determined the effectiveness of a set of antisense PS-ODNs directed against the glutathione S- transferase Mu1 (GSTM1) and Mu2 (GSTM2) isoforms of the rat GSTs play an important ... In summary, we selected several effective antisense ODNs against rat GSTM1 and GSTM2 from a set of 15 PS-ODNs in a novel, sensitive screening assay The assay discriminates between effective AS-ODNs...

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Báo cáo y học: "4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase" doc

Ngày tải lên : 09/08/2014, 13:21

... apoptotic response of chondrocytes to HNE is associated with decreased Bcl -2 expression and increased Bax expression This suggests that chondrocytes need to modulate the synthesis of at least several ... Values were expressed as the GSSG/[GSSG+GSH] ratio Western blot analysis Chondrocytes were incubated for increasing time periods (0 to 16 hours) with 30 μM HNE or with increasing concentrations of ... mortality, suggesting a role for Fas/CD95 and PARP in HNE-inducing cell death Second, in investigating the classi- cal markers of apoptosis, we obtained data showing that HNE induced caspase-8, -9,...

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Báo cáo y học: " Genetic polymorphisms in glutathione S-transferase (GST) superfamily and risk of arsenic-induced urothelial carcinoma in residents of southwestern Taiwan" ppt

Ngày tải lên : 10/08/2014, 05:21

... Glu208Lys (K208E) 0.0 125 rs11509438 C _113094 32_ 20 GSTO1 Exon6 A®C 0.0000 rs150 32 C _1174185_80 GSTO2 Exon2 5’UTR (-183) A®G Thr217Asn (T217N) — 0 .20 32 rs 229 723 5 C _ 322 31 42_ 1_ GSTO2 Exon5 A®G Asn142Asp ... association of this gene with extreme urinary profiles [28 -30] However, a recent study in Taiwan showed that the GSTO2 Asn142Asp (N142D) homo-variant was associated with an increased iAs% [31] A study of ... (GSTO) and arsenic (III) methyltransferase (AS3MT, or CYT19) are involved in classic arsenic methylation in a variety of animals including humans [24 -26 ] The GSTOs, including GSTO1 and GSTO2,...

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Báo cáo y học: " Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients" potx

Ngày tải lên : 12/08/2014, 15:20

... domain suggesting its role in the metabolism and maintenance of GSH levels in intact cells [20 ,22 ] Since GSH is one of the major antioxidants of the airways, it can be hypothesized that GSTO ... varied, being moderate/intense in 8/16 non-smokers, 9 /22 Statistical methods The statistical analyses were performed with the SPSS for Windows software (SPSS, Chicago, IL, USA) Continuous data were ... markers such as β-actin and some other antioxidant enzymes such as manganese superoxide dismutase in these specimens were negative (not shown) Western blot analysis for GSTO1-1 showed decreased levels...

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Báo cáo y học: "Anticitrullinated protein antibody (ACPA) in rheumatoid arthritis: influence of an interaction between HLA-DRB1 shared epitope and a deletion polymorphism in glutathione s-transferase in a cross-sectional study" ppt

Ngày tải lên : 12/08/2014, 15:22

... seronegative disease [2] Because of the limited sample sizes in subgroups of interest, our study did not include analyses of interactions of distinct HLA-DRB1 subtypes with GSTM1 Recent findings have shown ... complex interactions, including analyses of GSTM1-SE stratified by smoking status Future analyses of this sort with larger patient populations will be essential not only in replicating our findings ... categorized as heavy smokers [45] Differences in smoking exposure may be salient here, given findings from a separate North American study showing that SE-smoking interactions in the risk of seropositive...

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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

Ngày tải lên : 19/02/2014, 06:20

... properties [15] Hsp overexpression in COS-7 cells was assessed by transient transfection using vectors encoding either Hsp70, Hdj1, Hdj -2 or Hsp27 Hdj -2 is an isoform of Hdj-1 that has been previously ... decrease intracellular ROS levels [15,59] We also show here that the effect is speciﬁc to Hsp27, since it is not observed in the case of Hsp70 ⁄ Hsp40(Hdj-1) overexpression In cells expressing httEx1-72Q-EGFP, ... proteins in response to httEx1-72QEGFP expression (Fig 4A) Analysis of httEx1-103QEGFP-expressing cells revealed that in this case Hsp70 ⁄ Hdj-1 or Hsp27 were not efﬁcient in counteracting the increased...

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Báo cáo khoa học: Structure analysis of the ﬂavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the ﬂavin reductase family pdf

Ngày tải lên : 07/03/2014, 02:20

... Secondary structure elements of avoredoxin are shown on the lines of residue numbers Residues involved in binding of FMN are shown in red Residues shown in bold are aligned based on crystal structures ... NADH ()343 versus ) 320 mV) supports this explanation The second possibility is that the binding of NAD(P)H is sterically hindered by the surrounding residues Regarding this second possibility, when ... is also shown as a transparent model (D) Zinc ion-binding site Zinc ions are depicted as green spheres The remaining color codes are the same as in (A) and (B) D peaks at each of these sites...

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Analysis of Healthcare Interventions that Change Patient Trajectories doc

Ngày tải lên : 23/03/2014, 00:20

... service, and costs and benefits per instance of the service Table S. 1 shows our estimates of some of the effects of these five preventive services These estimates assume that the services are rendered ... residents ignores the cost of residing in the home, because these costs are deliberately not included in MEPS It assumes that a nursing home resident will visit the hospital or a physician s office ... to hospital stays with diagnoses that a physician identified for us as being most likely to be associated with ADEs Descriptions of hospital stays (including diagnoses and an identification of...

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Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular ﬁbronectin-mediated cancer cell adhesion pot

Ngày tải lên : 29/03/2014, 22:21

... likely representing a discontinuous FN-binding domain and favoring the mechanism of steric hindrance Interestingly, based on structural comparison and superimposition of rDPP IV (2GBC) and hDPP ... (control) with horseradish peroxidase-conjugated streptavidin Immunoﬂuorescent staining and ﬂuorescenceactivated cell sorting (FACS) analysis rDPP IV-expressing HEK293 cells were stained with lgÆmL)1 ... adverse effect in nude mice [15] It is likely that it causes distinct effects of cytotoxicity against DPP IV-expressing cancer cells and normal cells Altogether, we propose a hypothesis that...

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báo cáo sinh học:" Community characteristics that attract physicians in Japan: a cross-sectional analysis of community demographic and economic factors" potx

Ngày tải lên : 18/06/2014, 17:20

... taking into account distribution analysis of physicians against these parameters enables us to obtain a clearer sense of the demand/supply balance of physicians in communities, and to ascertain ... necessarily hold true [21 ,22 ] A substantial proportion of patients cross county borders to visit their physicians [21 ,22 ] Past studies in the United States revealed that the percentage of physician ... proportion of clinics was 13.7% to 26 .3% [20 ] Thus, most medical services are provided during business hours The data of Hong et al support our reported results Based on the population data of United States...

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Báo cáo sinh học: "Analysis of the human cytomegalovirus genomic region from UL146 through UL147A reveals sequence hypervariability, genotypic stability, and overlapping transcripts" ppt

Ngày tải lên : 19/06/2014, 08:20

... changes but instead occur as defined sets of amino acid substitutions suggests that there may be selection based on virus and/or host functional constraints Variability of host factors such as CXCR2 ... provides further evidence for 3' co-terminal transcripts, because there is a single consensus sequence at bp 12, 563 just downstream of UL1 32 The next closest signals are 2. 8 kb upstream and 2. 3 ... UL146 sequence Some of these strains were also subjected to extensive passage in cell culture and still retained absolute sequence stability Of note in this regard is the stability of strain W1...

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Báo cáo hóa học: " Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that ?" doc

Ngày tải lên : 20/06/2014, 01:20

... http://www.virologyj.com/content/5/1 /24 Figure Surface plasmon resonance analysis of the NS5A(Δ 32) :Fyn SH3 domain interaction Surface plasmon resonance analysis of the NS5A(Δ 32) :Fyn SH3 domain interaction SPR analysis was performed ... Figure 1a shows a schematic of the expressed proteins and figure 1b western blot analysis and Coomassie Blue staining of various stages in the purification process We established a two stage purification ... expressed truncated forms showing the locations of the N-terminal amphipathic helix and the three domains (b) AntiNS5A western blot analysis and Coomassie staining of the purification of NS5A(Δ 32) ...

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báo cáo hóa học:" High rate of loss to clinical follow up among African HIV-infected patients attending a London clinic: a retrospective analysis of a clinical cohort" ppt

Ngày tải lên : 20/06/2014, 08:20

... ethnicity/migrants [21 ,22 ], younger age [22 -24 ], a CD4 cell count of less than 20 0 cells/ mm3 [22 ,24 ] or a high CD4 count being associated with retention in care [25 ,26 ], non-use of HAART [24 ,27 ,28 ], a ... [21 ,22 ,24 ,27 ,28 ] or absence of an AIDS-defining illness as predictors of loss to follow up [24 -26 ] Therefore, among both HAART recipients and nonrecipients, it is the most vulnerable patients ... widespread use of HAART [23 ,25 ,26 ] or in research cohorts [27 -29 ] Furthermore, since many clinics not have established systems for identifying patients who are lost to follow up (LFU), there is...

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Báo cáo hóa học: " Research Article Multirate Formulation for Mismatch Sensitivity Analysis of Analog-to-Digital Converters That Utilize Parallel ΣΔ-Modulators" pdf

Ngày tải lên : 22/06/2014, 19:20

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