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qpcr assay targeting prot6e and inva genes for fast and accurate detection of salmonella enteritidis

INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx

INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx

Nông nghiệp

... taken in the disposal of all incubated materials Scope This International Standard specifies a horizontal method for the detection of Salmonella, including Salmonella Typhi and Salmonella Paratyphi ... at 37 °C and leave for several minutes (approximately min) Add 0,25 ml of the reagent for detection of β -galactosidase and mix Replace the tube in the water bath set at 37 °C and leave for 24 ... tests for detecting Salmonella, and especially Salmonella Typhi and Salmonella Paratyphi, are only undertaken in properly equipped laboratories, under the control of a skilled microbiologist, and...
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Báo cáo khoa học:

Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx

Báo cáo khoa học

... than one Salmonella serovar was found in Table Comparison of results of sampling of 26 Salmonella infected broiler flocks at weeks of age, and at slaughter age by pairs of sock samples No of Salmonella ... slaughter age No of Salmonella positive samples at weeks of age 10 2 16 Numbers in the table show the number of flocks Acta vet scand vol 43 no 1, 2002 1 10 26 Procedures for detection of Salmonella ... used for, and results of comparisons between sampling of 26 Salmonella infected broiler flocks at weeks of age, and at slaughter age by pairs of sock samples Statistics Routine 5-sock-samples and...
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Báo cáo y học:

Báo cáo y học: "Biochip sensors for the rapid and sensitive detection of viral disease" pps

Báo cáo khoa học

... array capable of correctly identifying four of the orthopoxvirus species Similarly, arrays have been developed for the detection and distinction of hantaviruses [8] and are capable of distinguishing ... used for the identification of viral inhibitors [6], and in 2002 this idea was applied by Wang et al [4] to the detection and identification of viruses The authors [4] described the use of viral ... only to detection and identification but also to the monitoring of viral populations In 1999, we and colleagues [6] described the first viral DNA microarray for the temporal profiling of viral...
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Báo cáo hóa học:

Báo cáo hóa học: " cDNA targets improve whole blood gene expression profiling and enhance detection of pharmocodynamic biomarkers: a quantitative platform analysis" ppt

Hóa học - Dầu khí

... cut off for the analysis of PNA and no treatment samples The number of significant genes differentially regulated between 1% liver and 1% brain is equal to 97 for no treatment, 117 for PNA and ... correlate to globin addition The standard deviations were 0.36 for no treatment, 0.3 for PNA and 0.12 for cDNA (table 1) cDNA hybridization allowed for the detection of 1.4-fold change in expression ... included the down regulation of MYC and up regulation of GADD45B [24] Conclusions Blood is a critical tissue for the understanding of disease and the development of disease treatments It is a...
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Báo cáo hóa học:

Báo cáo hóa học: " Research Article A Simple Technique for Fast Digital Background Calibration of A/D Converters" potx

Báo cáo khoa học

... shows the bandwidth of the PRBS as a function of L For each value of L, we report the fraction of the Nyquist bandwidth in which 50% and 90% of the noise power is concentrated The former is a ... bandwidth fN of about 0.035% of the Nyquist bandwidth); for comparison, the same PRBS has been used as the random signal PN in a standard implementation of the calibration procedure The use of ... the histograms of the effective number of bits (ENOB) with and without calibration, for 100 Monte Carlo iterations, and Table reports the average value and standard deviation of ENOB and SFDR Figure...
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Chapter 078. Prevention and Early Detection of Cancer (Part 1) pps

Chapter 078. Prevention and Early Detection of Cancer (Part 1) pps

Sức khỏe giới tính

... identification and manipulation of the genetic, biologic, and environmental factors in the causal pathway of cancer Education and Healthful Habits Public education on the avoidance of identified ... to teach patients about the hazards of smoking, the features of a healthy lifestyle (including diet and exercise), use of proven cancer screening methods, and sun avoidance Smoking Cessation ... cancer Lung cancer and cancers of the larynx, oropharynx, esophagus, kidney, bladder, pancreas, and stomach are all tobacco-related The degree of smoke exposure, meaning the number of cigarettes smoked...
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Chapter 078. Prevention and Early Detection of Cancer (Part 2) ppt

Chapter 078. Prevention and Early Detection of Cancer (Part 2) ppt

Sức khỏe giới tính

... in 9.8% of women in the low-fat diet group compared to 12.4% of women on the standard diet In observational studies, dietary fiber lowers the risk of colonic polyps and invasive cancer of the ... increased risk for cancers of the breast, colon, prostate, and endometrium These cancers have their highest incidence and mortalities in western culture where fat comprises an average of 40–45% of the ... absorbing and inactivating dietary estrogenic and androgenic cancer promoters However, two large prospective cohort studies of >100,000 health professionals showed no association between fruit and...
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Chapter 078. Prevention and Early Detection of Cancer (Part 3) doc

Chapter 078. Prevention and Early Detection of Cancer (Part 3) doc

Sức khỏe giới tính

... gas Cancer of the lung, head and neck, nasal sinuses Nickel dust Cancer of the lung, nasal sinuses Phenacetin Cancer of the renal pelvis and bladder Polycyclic hydrocarbons Cancer of the lung, ... such as androgens, linked to prostate cancer, and estrogen, linked to breast and endometrial cancer The distinction between an initiator and promoter is sometimes arbitrary; some components of cigarette ... Acute myeloid leukemia, bladder cancer Androgens Prostate cancer Aromatic amines (dyes) Bladder cancer Arsenic Cancer of the lung, skin Asbestos Cancer of the lung, pleura, peritoneum Benzene...
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Chapter 078. Prevention and Early Detection of Cancer (Part 4) pdf

Chapter 078. Prevention and Early Detection of Cancer (Part 4) pdf

Sức khỏe giới tính

... risk of colorectal cancer compared to women taking placebo Of >16,600 women randomized and followed for a median of 5.6 years, 43 invasive colorectal cancers occurred in the hormone group and ... taking placebo and of per 1000 subjects per year for those taking β-carotene The ATBC and CARET results demonstrate the importance of testing chemoprevention hypotheses thoroughly before their widespread ... change in the risk of lung cancer for those taking β-carotene; fewer of its participants were smokers than those in the ATBC and CARET studies Chemoprevention of Colon Cancer Many of the current...
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Chapter 078. Prevention and Early Detection of Cancer (Part 5) pdf

Chapter 078. Prevention and Early Detection of Cancer (Part 5) pdf

Sức khỏe giới tính

... increase in risk of endometrial cancer, stroke, pulmonary emboli, and deep vein thrombosis was noted Tamoxifen has been approved by the U.S Food and Drug Administration for reduction of breast cancer ... Trial randomly assigned men ≥55 years at average risk of prostate cancer to finasteride or placebo After years of therapy, the incidence of prostate cancer was 18.4% in the finasteride arm and 24.8% ... Prevention A number of infectious agents cause cancer Hepatitis B and C are linked to liver cancer, some human papilloma virus (HPV) strains are linked to cervical and head and neck cancer, and Helicobacter...
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Chapter 078. Prevention and Early Detection of Cancer (Part 6) ppsx

Chapter 078. Prevention and Early Detection of Cancer (Part 6) ppsx

Sức khỏe giới tính

... includes a biopsy and pathologic confirmation A number of genes have been identified that predispose for a disease, and many more will be identified in the near future Testing for these genes can define ... may be ideal candidates for chemoprevention and screening; however, efficacy of these interventions in the high-risk population should be investigated Currently, persons at high risk for a particular ... asymptomatic, healthy persons, it should offer substantial likelihood of benefit that outweighs harm Screening tests and their appropriate use should be carefully evaluated before their use is widely encouraged...
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Chapter 078. Prevention and Early Detection of Cancer (Part 7) pps

Chapter 078. Prevention and Early Detection of Cancer (Part 7) pps

Sức khỏe giới tính

... population Assessment of Screening Tests Good clinical trial design can offset some biases of screening and demonstrate the relative risks and benefits of a screening test A randomized, controlled ... the question of generalizability to the target population Screening trials generally involve thousands of persons and last for years Less definitive study designs are therefore often used to ... more aggressive, and treatment outcomes are not as favorable An extreme form of length bias sampling is termed overdiagnosis, the detection of "pseudo disease." The reservoir of some undetected...
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Chapter 078. Prevention and Early Detection of Cancer (Part 8) potx

Chapter 078. Prevention and Early Detection of Cancer (Part 8) potx

Sức khỏe giới tính

... a Summary of the screening procedures recommended for the general population by U.S Preventive Services Task Force (USPSTF), the American Cancer Society (ACS), and the Canadian Task Force on Prevention ... Fair evidence to cervix, include in examination beginning of sexually active years after first women intercourse by age Yearly or 21 for standard Pap; every years with liquid test Pelvic examination ... stand-alone >40, yearly without mammography Mammography F: 40–75, F: ≥40, every 1–2 years every year F: 50–69, every 1–2 years (fair evidence) Complete skin examination Insufficient evidence for...
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Chapter 078. Prevention and Early Detection of Cancer (Part 9) potx

Chapter 078. Prevention and Early Detection of Cancer (Part 9) potx

Sức khỏe giới tính

... screening for BRCA1 and BRCA2 mutations and other markers of breast cancer risk has identified a group of women at high risk for breast cancer Unfortunately when to begin and the optimal frequency of ... 3/1000 for colonoscopy and 1/1000 for sigmoidoscopy Debate continues on whether colonoscopy is too expensive and invasive for widespread use as a screening tool in standard-risk populations DRE and ... sensitivity for fecal occult blood is increased if specimens are re-hydrated before testing, but at the cost of lower specificity The false-positive rate for rehydrated FOBT is high; 1–5% of persons...
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Chapter 078. Prevention and Early Detection of Cancer (Part 10) doc

Chapter 078. Prevention and Early Detection of Cancer (Part 10) doc

Sức khỏe giới tính

... screening for basal and squamous cell cancers and melanoma No prospective randomized study has been performed to look for a mortality decrease Observational epidemiologic evidence from Scotland and ... be offered screening and allowed to make a choice after being informed of potential risks and benefits (Table 78-3) A man should have a life expectancy of at least 10 years to be eligible for ... showed that rigorous screening of low-risk men for years leads to the diagnosis of prostate cancer in >12% of patients In addition, 15% of men who had normal DRE and PSA levels after years were...
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Báo cáo khoa học:

Báo cáo khoa học: "Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens" docx

Báo cáo khoa học

... 44 001 38 92 97 )07 = n( sniarts tnatsiseR % iloc E nicaxolforpiC nicaxolfornE senoloniuqoroulF elozaxohtemaflus-mirpohtemirT sedimanoflus detaitnetoP nicakimA nicymotpertsordyhid nicimatneG sedisocylgonimA ... morf derevocer setalosi eht fo lla ,seliforp ecnatsiser gurditlum ot drager htiW ylevitcepser ,)%97( locinehpmarolhc dna ,)%18( nicaxolforpic ,)%38( nicaxolforne ,)%38( nillicipma ,)%001( enilcycartetyxo ... eht roF ]42,91[ debircsed ylsuoiverp esoht ot lacitnedi erew 3-tac dna ,2-tac ,1-tac ,Almc fo noitacifilpma eht ni deyolpme stes remirp ehT nim rof C 27 ta pets noisnetxe na dna ,ces 54 rof C...
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Báo cáo khoa học:

Báo cáo khoa học: "Use of soft X-radiography for early non-destructive detection of floral differentiation in Douglas fir buds" pptx

Báo cáo khoa học

... of the plant of particular interest for ontogenetic studies; and (ii) to have a more accurate definition of the state or the development stage of organs and tissues for sampling, especially for ... about once every weeks (July 22 for tree number 1, July 31 for trees 1-5, August 13 for trees 1-5, August 22 for trees 1-3, August 26 for trees and 5, and September for trees 1-5) At each sampling ... number of buds on September (table II) The date of floral transformation of some of the buds in these radiographs was estimated as being the same as on the excised shoots For the population of buds...
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Báo cáo y học:

Báo cáo y học: " Global and unbiased detection of splice junctions from RNA-seq data" pptx

Báo cáo khoa học

... include identification of splice sites in uncharacterized transcripts, detection of long-range fusion transcripts, and detection of small indels in transcribed sequences About 12% of the predicted ... 11.6 million reads) and oocyte2 (23.5 million reads), and oocyte1 +2, a combination of all reads from the two samples These data consist of 50-bp reads, and the alignment was performed by using the ... whole-transcriptome-alignment software with anchor lengths in the range between 21 and 24 (see Methods for details) The highest number of uniquely mapped split reads was obtained for lengths 22 and 23 (see Table...
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báo cáo khoa học:

báo cáo khoa học: " Rapid and accurate pyrosequencing of angiosperm plastid genomes" doc

Báo cáo khoa học

... complements of coding genes, each containing 30 tRNA genes, rRNA genes, and 79 protein-coding genes (Table 3) Based on the presence of internal stop codons, two pseudogenes (ycf15 and ycf68) are ... genome map of Nandina domestica (Berberidaceae) Plastid genome map of Nandina domestica (Berberidaceae) Map of the plastid genome of Nandina domestica (Berberidaceae), showing annotated genes and introns ... fields of plant genetics and phylogenetics by dramatically expanding the amount of sequence data available to both Methods Fresh leaf material of Nandina and Platanus was collected on the campus of...
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