purification reconstitution and functional characterization of zinc transporter from rat renal brush border membranes

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Ngày tải lên : 18/02/2014, 18:20

... concentration of Rep245 prepared in 10 mm sodium phosphate (pH 7.4) was lm CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm ... plausibly involved in DNA recognition and binding and is positioned left of the active site [14] Rep245 is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension ... selection stringency and incorporation ﬁdelity [26] Thermal activity analysis of Rep245 revealed an optimal temperature of 65 °C, i.e 10 °C lower than the growth temperature of the natural host...

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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Ngày tải lên : 21/02/2014, 00:20

... presence of several concentrations of ATP (A) or AMP (B) and different concentrations of the inhibitor Each point is the mean of three separate trials Inhibition of AK by the specific inhibitor Ap5A and ... Asian and Mediterranean countries [15] This paper describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular characterization ... abundance of AK mRNA during the growth curve of promastigotes Functional characterization of recombinant AK The recombinant protein purified from the inclusion bodies and refolded in the presence of...

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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Ngày tải lên : 07/03/2014, 15:20

... fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP accumulated ... differential expression of isoforms of CRFR1 to precisely couple selectively activated phenotypic targets, we performed molecular characterization of newly described CRFR1 isoforms First, we tested ... lLÆmL)1 of donor beads and lLÆmL)1 of acceptor beads The signal was measured by Fusion a instrument (Packard BioScience, Meriden, CT, USA) cAMP concentration was recalculated from the standard...

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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Ngày tải lên : 07/03/2014, 21:20

... VBARP-L consisted of a 576-bp fragment absent from the shorter VBARP-S and contained a 114-bp UTR (Fig 2A) Intron and exon analysis of VBARP revealed that VBARP-L and VBARP-S contain 11 and exons, respectively ... lymph node, and muscle expressed relatively low levels of ANKHD1 isoform In the case of VBARP-L isoforms, spleen showed the highest level (3.89 ratio) followed by lung, lymph node and kidney Interestingly, ... Expression and biochemical characterization of VBARP The predicted sizes of the proteins encoded by VBARP-L and VBARP-S are 69 and 49 kDa, respectively To test this, C-terminal V5-tagged VBARP-L and...

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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebraﬁsh SULT1 sulfotransferases docx

Ngày tải lên : 08/03/2014, 02:20

... Detection of zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and ... The concentration of the divalent metal cations tested was mM, and the concentration of NaCl tested was 10 mM 2410 T Sugahara et al (Eur J Biochem 270) Expression of sebrafish SULT1 ST1 and SULT1 ... dose-dependence of the regulation of the activity of the zebrafish ST by these divalent metal cations and their modes of action Fig Effects of divalent metal cations on the sulfating activity of the zebrafish...

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Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

Ngày tải lên : 16/03/2014, 05:20

... stiff and tough, and protection from microbial degradation This multifunctionality, together with the insoluble ⁄ sticky and complex nature of the adhesive, have hindered any detailed analysis of ... molecular masses and isoelectric points of the mature Table Amino acid compositions of various cp-19ks and their deviations from standard compositions The amino acid compositions of mature cp-19ks ... composition calculated from the deduced sequence b The amino acid composition analyzed by amino acid analysis of the numbers of Asp and Asn d Sum of the numbers of Glu and Gln 4338 c Sum FEBS...

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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

Ngày tải lên : 16/03/2014, 06:20

... composed mainly of a helices (Figs and 2) The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) anking a ... overall morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar in the absence or presence of calcium ions Calcium ... it may be concluded that EF-hand of the Iba proteins is not capable of functional Ca2+ binding Function of EF-hand In both Iba1 crystal structures, the loops of EF-hand adopt essentially the same...

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Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

Ngày tải lên : 24/03/2014, 03:21

... 5A) A BS3 concentration of 0.16 mM was sufficient to generate the accumulation of a larger protein form With increasing concentrations of BS3, the disappearance of the monomer band corresponded ... mass of % kDa The removal of the carbohydrate moiety had no effect on any of the characteristics of smelt AFP that were investigated However, the possibility of alternative and untested roles of ... with a kmax of 343 nm from % 30 lM samples of both untreated (Fig 4A) Fig Effect of Ca2+ on the intrinsic fluorescence of deglycosylated and untreated smelt AFP (A) Emission spectra of intact (untreated)...

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Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

Ngày tải lên : 30/03/2014, 04:20

... standard After extraction of the steroids and evaporation of the chloroform phase, the steroids were resuspended in 200 lL acetonitrile and separated on a Jasco reversed phase HPLC system of ... interspecies conversion rates However, the alterations may also be explained by the broader substrate-binding spectrum of bCYP11B1 resulting from the combination of the functions of CYP11B1 and CYP11B2 within ... be attributed to an anisotropy of the porphyrin absorption band [22] Upon addition of substrate, the signal of the positive CD band at 290 nm and the negative band at 386 nm decreased (Fig 4B)...

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Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

Ngày tải lên : 30/03/2014, 04:20

... This is the ﬁrst report, for eukaryotes, of cDNA cloning and functional characterization of d-AAT acid aminotransferase in A thaliana tion rate of main roots and hypocotyls appeared to be greater ... activities were determined, and are shown in Table In the transamination conversion of amino acid to a-ketoglutarate, Km and Vmax values for d-Asp and d-Ala were 2.3 and mm, and 2.5 and 5.0 lmolÆmin)1Æmg)1 ... d-amino acids, was cloned and characterized This represents the ﬁrst cDNA cloning and functional characterization of a d-AAT of eukaryotic 1196 origin Further characterization of this d-AAT is necessary...

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Báo cáo khoa học: Identiﬁcation and functional characterization of an aggregation domain in long myosin light chain kinase ppt

Ngày tải lên : 30/03/2014, 04:20

... existence of potentially functional domains in this extension is of importance for elucidating the roles of L-MLCK Some functional domains have already been identiﬁed in L-MLCK and, on the basis of ... muscle degeneration As mentioned above, the aggregate-containing ﬁbers were of small size and irregular morphology, both typical of degenerative pathology In order to assess the extent of functional ... Localization of 4Ig aggregates in mitochondria and measurements of mitochondrial respiratory activities (A) Transmission electron microscopy images of 4Ig aggregates in mitochondria of the EDL and diaphragm...

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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

Ngày tải lên : 30/03/2014, 08:20

... phosphorylation, and P2Y1 and P2Y12 receptor protein expression and functional activity Examination of the effects of speciﬁc pharmacological agents (a single agonist and two speciﬁc antagonists of P2Y1 and ... devoid of serum for up to 96 h and examined the effect of such long-term serum starvation on cellular morphology, adhesion, proliferative and differentiation state, basal level of ERK1 ⁄ and Akt ... [phosphatase and tensin homolog (PTEN)] [3] PTEN dephosphorylates the 3¢ position of phosphoinositides and acts as an antagonist of PI3-K, resulting in a reduction of the concentration of this kinase...

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Báo cáo khóa học: Puriﬁcation and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

Ngày tải lên : 30/03/2014, 13:20

... 30 s, and 68 °C for min, yielding a single band of predicted size The DNA band was digested with NdeI and XhoI, and cloned into the NdeI and XhoI sites of pET22b The H151A mutant protein of SMC ... Biochem 271) Isolation of the bacteria from antlion M bore and preparation of the culture broth The antlions were sterilized with an aqueous 70% ethanol solution, and one of the mandibles was pulled ... SDS/PAGE of native SMC and the recombinant protein SDS/ PAGE of native SMC puriﬁed from the culture broth of B cereus (A; 10% gel) and the recombinant protein, which was expressed by E coli and subsequently...

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Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc

Ngày tải lên : 31/03/2014, 09:20

... insensitivity of front-end desaturases and elongases to the presence of an x3-double bond and of x3-desaturases to the structure of the carboxyl end of the fatty acids, the existence of separate x3- and ... report the cloning and functional characterization of a D5- and a D6-fatty acid desaturase from P tricornutum Both contain the typical features of membrane-bound desaturases and a cytochrome b5 ... conﬁrm the D5- and D6-regioselectivity of PtD5p and PtD6p, respectively (Fig 3), and to determine each substrate speciﬁcity (Table 1) PtD6p desaturated 25–30% of both 18:2D9,12 and 18:3D9,12,15...

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Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf

Ngày tải lên : 08/08/2014, 14:20

... generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5’region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation codon was isolated from ... region of GS1a gene The clone includes 980 bp upstream of the functional ATG So far, very few studies involving genomic clones from gymnosperms have been reported in the literature [4,16,18], and ... tissues of developing seedlings and the level of the transcript was affected by developmental and light conditions [9] Here, we present the DNA sequence of a partial genomic clone containing exons of...

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Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps

Ngày tải lên : 09/08/2014, 14:22

... of MNC isolated from SF and paired PB samples of 25 JIA patients, as well as from PB samples of 20 age-matched controls CD19+ cells were less numerous in SF than in paired PB (SF percentage of ... chemokine receptor CXCR1, CXCR2, and CXCR3 mAbs from Serotec Inc (Raleigh, NC, USA); and PE-conjugated anti-CXCR4 and CXCR5 mAbs from R&D Systems Cell staining and flow-cytometric analysis were ... such as CD80 and CD86 and presenting antigens to T cells [9] With this background, we here address the immunophenotypic and functional characterization of synovial B cells from JIA patients, with...

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Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot

Ngày tải lên : 10/08/2014, 05:21

... (M), and local concentrations (C) of different species were shown The local concentration of SV was from the discrete distribution of SV and that of SE was from the SE experiments The concentration ... Expression and Purification of ApoE Proteins Protein induction and purification procedures have been described previously [22,23] Typical yields of the apoE(72-166) proteins were 5-10 mg after purification ... level of p = 0.95, a best fitted average anhydrous friction ratio (fr), a resolution value N of 200, and sedimentation coefficients between and 20 S For the data fitting of apoE-(72-166) in PBS and...

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báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx

Ngày tải lên : 11/08/2014, 11:21

... Ler, Landsberg erecta Page 12 of 13 Additional file 3: Growth of AtERF13, RAP2.4 and RAP2.4L knockout and RNAi lines RNAi lines of AtERF13 and knockout lines of RAP2.4 and RAP2.4L were prepared as ... #25 and #31 denote RNAi lines (B) Single or double knockout (KO) lines of RAP2.4 and RAP2.4L Top left, expression levels of RAP2.4 and RAP2.4L in the single knockout lines of RAP2.4 (RK) and ... a positive regulator of ABA and sugar responses [35] DREB2C and maize DBF1 are also positive regulators of ABA response [36,37] On the other hand, AtERF7 [38], ABR1 [39] and AtERF4 [34] are ERF...

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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx

Ngày tải lên : 12/08/2014, 03:21

... types of cis-elements and orthologous transcription factors However, the identification and functional characterization of these types of promoters in fruit trees is lacking We have demonstrated ... regulation of these promoters in peach and Arabidopsis Figure selected accuracy of the predicted expression patterns of Evaluation of the genes by RT-PCR Evaluation of the accuracy of the predicted ... these promoters Functional characterization of these promoters (stably in A thaliana and transiently in peach fruits) demonstrates that these are peach cold-inducible promoters and that there...

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báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt

Ngày tải lên : 12/08/2014, 03:21

... gene in S lycopersicum and S habrochaites The mRNA levels of CYC-B gene in leaf and flower, and at different stages of fruit development of S lycopersicum cv Pusa Ruby and S habrochaites genotype ... expression, and thus can be a valuable tool in understanding carotenoid metabolism in tomato Methods Isolation and cloning of ShCYC-B promoter Isolation of 5' flanking region of CYC-B gene from S ... 365 nm and emission at 455 nm The amount of 4-MU was determined from a standard curve Protein concentrations of the samples were determined using Bradford reagent (BioRad, Hercules, CA) and BSA...

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