protocol for expansion of undifferentiated human embryonic and pluripotent stem cells in suspension

Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

Ngày tải lên : 18/06/2014, 16:20

... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... of resuspended cells and refrigerated in the dark for 15 (4-8) C Cells were washed in ml of PBS with 0.5% BSA and resuspended in 100 ul of buffer for analysis Flow cytometry was performed using ... required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated...

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Báo cáo y học: "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity" pps

Ngày tải lên : 14/08/2014, 08:20

... seen in primitive endoderm and extraembryonic endoderm cell lines (XEN cells) [29,30], and can be induced in ESCs or in the inner cell mass (ICM) by knocking out or downregulating expression of ... centrifugation and cells were fixed in Genome Biology 2007, 8:R163 information Karyotyping interactions Cells were collected by trypsinization, washed twice with PBS and fixed in 4% paraformaldehyde for ... Auburn, CA), and cells replated at 10 cells/ well Expansion was done by trypsinizing (0.05% trypsin, Cellgro) the cells and replating them every days at a density of 100-200 cells/ cm2 Cells at population...

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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair

Ngày tải lên : 12/09/2015, 09:42

... bodies composed of -actinin, the N-terminus of titin, nebulin, and telethonin contribute to the polarized organization of the thin actin filaments to form I-Z-I bands in developing sarcomeres11, ... Likewise, M-band proteins such as myomesin, M protein and the C-terminus of titin play a significant role in incorporating the myosin thick filaments into regular A bands19-25 Importantly, the coincident ... differentiation of stem cells into well-defined lineages in vitro, by introducing ECM molecules into the culture milieu In addition, cell-ECM interactions may promote the proliferation of these adult stem cells...

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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps

Ngày tải lên : 12/08/2014, 15:22

... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition, ... addition of UC-MSCs maintained such inhibitory effects, suggesting that the transplantation of these cells is practicable and effective for treatment of RA Interestingly, the invasive behavior of FLSs...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole ... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Commission for the Study of Ethical Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death...

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Báo cáo Y học: Monitoring the structural consequences of Phe12 ﬁ D-Phe and Leu15 ﬁ Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf

Ngày tải lên : 17/03/2014, 10:20

... 84% for studies carried out in 40% TFE in an H2O mixture [38] According to studies of CRH binding to single bilayer egg phosphatidylcholine vesicles, the development of the intramolecular interaction ... torsion angles for the mainchain conformation of Aib15 in the family of 40 energy minimized structures are calculated as u ¼ )59.6° ± 1.5 and w ¼ )31.9 ± 1.5, while for the mean energy minimized structure ... of the linear structure of human/ rat CRH [15] in the region of Aib residue probably acts as a helix-breaking signal in the region of Val18–Leu19 (3JHNHa 8.0 and 6.0 Hz, respectively) justifying...

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Báo cáo y học: "Apigenin, a non-mutagenic dietary flavonoid, suppresses lupus by inhibiting autoantigen presentation for expansion of autoreactive Th1 and Th17 cells" potx

Ngày tải lên : 09/08/2014, 14:20

... were fixed in 10% formalin and paraffin-embedded To determine the extent of renal disease, sections were stained with hematoxylin and eosin and periododic acid-Schiff and graded in a blinded fashion ... concentration of 30 M, apigenin induced twofold more apoptosis in DCs and macrophages than in T and B cells At a concentration of 10 M, apigenin did not induce significant apoptosis of T cells, but B cells, ... Conclusions Apigenin inhibits autoantigen-presenting and stimulatory functions of the APCs necessary for activation and expansion of autoreactive Th1 and Th17 cells and B cells in lupus Apigenin also causes...

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Báo cáo y học: " Expansion of the human mitochondrial proteome by intra- and inter-compartmental protein duplication" ppsx

Ngày tải lên : 09/08/2014, 20:21

... 2009, tein functional module in human Three enzymes participating in ornithine metabolism can be found in mitochondria in human and ureotelic mammals, but not in yeast: OTC, CPSase I and P5CS Of these, ... origin of a domain responsible for the binding with Ca2+, as the binding site is created upon the formation of a complex with the E2 subunit of the PDH complex and requires the lipoyl groups of ... ancestor of human and yeast, and a subsequent gain of mitochondrial localization We hypothesized that these proteins can constitute gains of mitochondrial localization in the human lineage To...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and ... isolation and expansion Cytotherapy 75 Du F, Zhou Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological ... stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991;Mesenchymal stem...

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Frequency of Routine and Flooding-stage Observations for Precise Annual Total Pollutant Loads and their Estimating Method in the Yodo River

Ngày tải lên : 05/09/2013, 09:38

... period in 2005, mostly winter, was stable Fig shows the changes in TOC and DOC concentrations with the change of flow in 2005, and Fig shows the changes in TOC and T-COD loadings with the change of ... events and storm runoff events which bring increases in pollutant loads during flooding-stages in large river catchments As the traverse water mixing in the wide Yodo River was relatively weak during ... flooding stage Therefore, we conducted continuous observations at three transverse points over the river during five periods of flooding in the routine observation period The frequency of flooding-stage...

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Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx

Ngày tải lên : 07/03/2014, 12:20

... identified in fungi, in mammals and in plants, including A thaliana [2,4,17,18] In fact, six different UXS isoforms have been identified in A thaliana and they can be classified into two types AtUXS1 and ... resulted in production of a protein of approximately 54 kDa in the TOY1 and TOY3 strains, whereas the protein encoded by AtUXS3 was detected as a 40-kDa band within the TOY2 and TOY3 strains (Fig ... strain To gain a better understanding of how the amount of UDP-sugars was regulated in the cells, we analyzed several properties of the recombinant AtUGD1 The substrate saturation kinetics of...

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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt

Ngày tải lên : 16/03/2014, 16:20

... remnants are able to induce foam cell formation in human monocyte-derived macrophages (HMDM) [21] and in human and murine macrophage cell lines [22–24], and that, in striking contrast to LDL, ... effect by comparing the in uence of CRLPs and CRLPs containing probucol (pCRLPs) on the uptake of the particles by the cells, intracellular lipid synthesis, and the expression of mRNA for genes believed ... effects of CRLPs and pCRLPs on any of the genes investigated We have reported the in uence of CRLPs containing lycopene (lycCRLPs) on the expression of mRNA for LDLr, LRP, ACAT1 and DGAT1 in THP-1...

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Transfusion Medicine Made Easy for Students of Allied Medical Sciences and Medicine Authored ppt

Ngày tải lên : 23/03/2014, 15:20

... made up of an intact light chain and the amino –terminal end of the heavy chain linked by a disulphide bondThe Fab portion is predominantly carbonhydrate and contains specific antigen binding ability ... infections Eosinophils have Fc receptors for IgE and binding of eosinophils to IgEcoated helminths results in killing of the parasite IgE does not fix complement Transfusion Medicine Made Easy for Students ... and N) Anti humanglobulin (AHG) reagent Anti -human globulin reagent are antibodies produced against human globulin (IgG) and will detect the presence of human globulin coating on red cells (sensitized...

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Serological Diagnosis of Certain Human, Animal and Plant Diseases Edited by Moslih Al-Moslih potx

Ngày tải lên : 23/03/2014, 16:21

... eliminate the infection and therefore, production can be affected for the life of the flock Vaccination of laying hens is performed at 12 weeks of age and delivered in the drinking water (Usman and Diarra ... among siblings and children under the age of In developing countries infection occurs in the first years of life and increases successively involving almost 90% of the 50year-olds In developed ... vary according to the type of infection and viral etiology We have to take into consideration a stage of infection, incubation period, beginning of clinical appearance and dynamic of immune response...

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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc

Ngày tải lên : 24/03/2014, 00:21

... in lane 1, runs only marginally above the hA2aR fusion protein and is the main band in lane In this puri®cation, 7% of speci®c ligand binding sites loaded onto the XAC-agarose were detected in ... obtained, namely LysIle-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the mature maltose-binding protein Binding of the fusion protein to the IMAC gel in buffer containing ... ef®cient extraction and maintain structural integrity We used radioligand binding assays to monitor hA2aR integrity and stability Approximately 70±90% of speci®c [3H]ZM241385 binding sites were solubilized...

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Báo cáo khoa học: Benzo[a]pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for a common food pollutant doc

Ngày tải lên : 30/03/2014, 11:20

... carcinogenesis in rodents and 20-fold lower than those causing alterations of the EGF receptor in cultured human uterine cells, RL95-2 [34] In vivo the maximal inhibitory effect on epinephrine-induced ... m ol in e m ut a b ep in ep hr in e 0.5 ACTH-induced FFA release (mM/mg prot) C Norepinephrine-induced FFA release (mM/mg prot) B Epinephrine-induced FFA release (mM/mg prot) Epinephrine-induced ... between epinephrine and saline incubations (A) (B) Effect of 15 preincubation of human adipocytes with either B[a]P (j) or saline (h) followed by 45 incubation with ANP In the absence of pharmacological...

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Báo cáo sinh học: "Safety and pharmacokinetics of recombinant human hepatocyte growth factor (rh-HGF) in patients with fulminant hepatitis: a phase I/II clinical trial, following preclinical studies to ensure safety" ppt

Ngày tải lên : 18/06/2014, 19:20

... each), intravenously for 14 days, and urinary excretion of albumin and protein was measured before (day 1), during (days and 14), and and 14 days after HGF administration Repeated doses of rh-HGF induced ... decrease in BP during rh-HGF infusion and renal toxicity induced by repeated rh-HGF dosing, including an increase in urinary excretion of albumin, were potential adverse events in a human study In ... of 12 (day) Figure Repeated dose of rh-HGF induced an increase in urinary excretion of albumin and protein in rats Rats were administered rhHGF, 0.4 (A), 1.0 (B), and 4.0 mg/kg/day (C) (n = for...

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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc

Ngày tải lên : 20/06/2014, 07:20

... Cumulus cells were lysed in Laemmli sample buffer and protein extracts were stored at -80°C until use After denaturing by boiling for min, 10 ul of each samples containing equal amounts of protein ... expressed in porcine granulosa cells in vivo in response to hCG administration [12] Thus, in vivo during the ovulation process, LH induces EGF-like factor expression in granulosa cells and the ... remained unclear during in vitro maturation of porcine COCs http://www.ovarianresearch.com/content/2/1/20 The binding of FSH and/ or LH in granulosa cells to its own receptors led to rapidly and...

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