overexpression and expression of mutant ank on the hypertrophic phenotype in transduced atdc5 cells
Báo cáo y học: "Transition of healthy to diseased synovial tissue in rheumatoid arthritis is associated with gain of mesenchymal/fibrotic characteristics" pdf
Ngày tải lên :
09/08/2014, 08:23
... was involved in conception of the study and participated in the design of the study CL was involved in conception of the study and participated in the design of the study CPJV was involved in ... participated in the design of the study and was involved in interpretation of the data REMT was involved in conception of the study and participated in the design of the study and helped to draft the ... the design of the study and interpretation of data BJW was involved in the design of the study and interpretation of data and gave technical support TWJH was involved in conception of the study,...
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Báo cáo y học: "Collagen-specific T-cell repertoire in blood and synovial fluid varies with disease activity in early rheumatoid arthritis" pot
Ngày tải lên :
09/08/2014, 13:22
... contributions FR has made substantial contributions to the conception and design of the study, and analysis and interpretation of the data He drafted the manuscript and gave final approval of the version ... Proliferation assay As a measure of the inhibitory capacity of Treg cells contained in each sample, we measured the effect of CD4+CD25+ depletion on the proliferative response of autologous T cells The ... presence of TCR expanding in response to stimulation of PBMCs with huCollp261–273 As shown in Figure 2, the total number of T cells expanding was no different among control individuals and RA patients...
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Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
Ngày tải lên :
09/08/2014, 14:20
... d, demonstrating more intense GAG staining in the deep non-lesioned OA cartilage regions and less intense staining in lesioned cartilage (Figure 1d) compared with the superficial non-lesioned ... sampling locations at the lesion and remote from the lesion yielding cartilage specimens from deep and superficial non-lesioned areas (b) Comparison of modified Mankin scores for both lesioned and ... This is exactly the result we find The authors of that study further found that the hyaluronanbinding (G1) domain of aggrecan is retained early in the disease process and then finally released...
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Báo cáo y học: "Plasma and synovial fluid microRNAs as potential biomarkers of rheumatoid arthritis and osteoarthritis" pot
Ngày tải lên :
12/08/2014, 12:20
... Cloning of PCR products and generation of standard curve To verify the PCR products and to generate standard curves of miRNAs, thymine adenine (TA) cloning was performed The resultant reaction ... TJC These results indicate that miR-132 might be involved in the systematic condition of patients with joint inflammation On the other hand, miR-16, miR-146a, miR-155 and miR-223 were higher in ... miRNAs in the condition medium of each cell or tissue of RA and OA A, FLSs of RA (n = 4) and OA (n = 5) were cultured in serum-free medium for 48 h Concentrations of miRNAs in each conditioned...
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Báo cáo y học: "Synovial effusion and synovial fluid biomarkers in psoriatic arthritis to assess intraarticular tumor necrosis factor-a blockade in the knee joint" doc
Ngày tải lên :
12/08/2014, 14:22
... was insufficient to control local knee disease activity The expression of proinflammatory cytokine/chemokine at baseline in the SF of the PsA patients is consistent with previous findings in the ... Switzerland Authors’ contributions UF was responsible for the study concept and design, analysis and interpretation, and drafting the manuscript PS participated in the design of the study and performed ... regulation of type 17 T-helper (TH-17) cells in the pathogenesis of psoriasis [18,19] Secretion of cytokines, such as IL-22 and IL-17, could, moreover, induce keratinocyte proliferation and skin inflammation...
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Báo cáo y học: "Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)" ppt
Ngày tải lên :
12/08/2014, 15:22
... pilot trial of a single injection of the short-acting intra-articular IL-1Ra therapy, anakinra, administered at the time of presentation to the clinic [20] The samples used in this study were ... suggesting a systemic response to acute injury rather than a signal knee specific response In the acute injury phase, the duration and/ or level of elevation of CRP in the serum may be a useful indicator ... together, these results demonstrate that the cartilage within the injured joint responds to the initial acute injury with a wave of proteoglycan and non-collagenous protein loss However, as the concentrations...
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Báo cáo y học: "Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis" doc
Ngày tải lên :
09/08/2014, 01:23
... fraction in comparison with the starting material (Fig 2, top panel) Furthermore, the average intensity of the ~170-kDa band in the FT was 61.5 ± 44.2% of the corresponding value in the starting ... starting material in the remaining eight RA samples, with little or no staining for ~200+-kDa species in the FT fractions (Fig 2, bottom) The average intensity of staining of the ~170-kDa band in ... staining of a 45-kDa GBDcontaining fragment (Fig 3b) R335 Arthritis Research & Therapy Vol No Peters et al Table Species of fibronectin bearing the N-terminal heparin-binding domain in samples of...
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Báo cáo y học: "Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils" doc
Ngày tải lên :
09/08/2014, 10:20
... Isolation of mononuclear cells and granulocytes from human blood Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at g Scand ... RA spontaneously expressed RANK-L, OPG, and RANK proteins (Figure 2), we next investigated the effect of SF on the expression of these genes by incubating healthy human blood neutrophils in the ... Conclusion Direct evidence is provided for the differential expression of proteins of the RANK/RANK-L pathway in neutrophils in a noninflammatory versus inflammatory context Moreover, signaling...
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Báo cáo y học: "Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 01:23
... because the concentration of cells in the SF and the levels of cell death vary with the severity of arthritis and the use of cytotoxic drugs Nevertheless, it does demonstrate that inflamed joints contain ... the infiltration of new inflammatory cells, the death of resident cells, and the continuous secretion of DNA adducts, all of which contribute to the inflammatory outcome High systemic levels of ... conditions The optimum concentration of MgCl2 for PCR amplification of mtDNA was determined by analyzing PCR reactions containing various concentrations (2.0, 3.0, and 4.0 mM) of MgCl2 In addition,...
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Báo cáo y học: "Osteogenic protein 1 in synovial fluid from patients with rheumatoid arthritis or osteoarthritis: relationship with disease and levels of hyaluronan and antigenic keratan sulfate" ppt
Ngày tải lên :
09/08/2014, 08:22
... interpretation, and writing of the data BSF was involved in the acquisition, analysis and interpretation of the data and in drafting the manuscript MM was involved in sample acquisition and analysis of the ... was involved in the development and adaptation of the ELISA method for SF and in the acquisition of data CAM was involved in data acquisition EJMAT was involved in interpretation of the data and ... chondroitinase ABC (0.1 U/ml); lane 4, combination of hyaluronidase (50 units/ml) and chondroitinase ABC (0.1 U/ml) The numbers on gels represent the size of the protein bands of interest and incubated...
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Báo cáo y học: " Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections" pdf
Ngày tải lên :
09/08/2014, 08:23
... against infection Inhibition of its activity could therefore be anticipated to augment the risk of infection in patients with RA The reasons for the predominance of S aureus in SA and the mechanisms ... components The profile was similar to that induced by a combination of IL-1/TNF, which might indicate the involvement of an inflammatory cytokine-mediated pathway in the observed induction of ... express integrins, MMPs, and proinflammatory cytokines There was no mention regarding MMP expression after TLR blockade, and it remains unclear whether TLR is involved in MMP expression in a more...
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Báo cáo y học: " Effects on osteoclast and osteoblast activities in cultured mouse calvarial bones by synovial fluids from patients with a loose joint prosthesis and from osteoarthritis patients" pdf
Ngày tải lên :
09/08/2014, 10:20
... responsible for the bone-resorbing activity Expression and importance of RANKL/RANK/OPG in SFinduced bone resorption Semi-quantitative RT-PCR assessments of the mRNA expressions of rankl and ... preparing the draft of the manuscript PL and AL were responsible for the experiments and analysis of gene expressions AO was actively involved in the initial work leading to the hypothesis on bone-resorbing ... Research & Therapy Vol No Andersson et al Introduction Aseptic loosening of a joint prosthesis is associated with remodelling of bone tissue in the vicinity of the prosthesis Histopathological and morphometric...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 10:21
... most chemokine receptors were downregulated in SF PMN, supporting the notion of a differential modulation of chemokine receptors and their ligands during chronic inflammation These findings suggest ... inflammatory milieu of the joint Differential expression of chemokine receptors in SF PMN To estimate changes in the responsiveness of SF PMN to chemokine ligands, expression levels of chemokine receptor ... irradiation of PMN on their own abolished the production of TNF-α in co-culture with EA-hy.926 cells, indicating that PMN are the predominant source of TNF-α in co-cultures The results therefore...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
Ngày tải lên :
09/08/2014, 14:22
... and JS participated in the design and coordination of the study, and drafted the manuscript MR has assisted in writing the manuscript AH and AS analyzed microbiological and sequencing data, and ... aAccession number of the bacterial species in the EMBL database bLength of alignment on which the 16S rDNA inserted sequence and the corresponding sequence in the database are similar cIn the '% similarity' ... study in order to avoid any contamination [15] From a practical point of view, it is important to notice that we did not surgically incise the skin at the site of the aspiration thus avoiding contamination...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
Ngày tải lên :
19/02/2014, 02:20
... shown in Fig 10C, confirm the higher hydrophobicity in human ACMSD of the region surrounding the metal active site and the Arg47 likely involved in substrate binding Discussion In this study, the expression ... elevation of quinolinate in serum, urine, cerebrospinal fluid and peripheral tissues [38–41] On the other hand, we found significantly lower levels of human ACMSD expression in liver and in brain than ... of the metallocofactor in human ACMSD might also be inferred by our study on the enzyme pH dependence, showing that the increase of the concentrations of succinate and 4-morpholineethanesulfonic...
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Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
Ngày tải lên :
19/02/2014, 07:20
... based on the original PCR clone and gave the remaining part of the coding region The coding part of the Y6 gene is contained within one exon and encodes a protein of 374 amino acids displaying the ... an AUG context that agrees with the consensus sequence for initiation of translation Phylogenetic analyses Isolation and sequencing of the chicken Y7 gene and cloning into the expression vector ... polymerase, on an ABI PRISM 310 Genetic Analyzer, and found to be identical to the genomic sequence The expression construct contains one upstream in- frame methionine codon (immediately after the cloning...
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Báo cáo khoa học: Genomic organization, tissue distribution and deletion mutation of human pyridoxine 5¢-phosphate oxidase pptx
Ngày tải lên :
23/03/2014, 12:20
... regulation of PLP could be controlled by several factors The synthesis of PLP requires the joint action of PDXK and PNPO, and the PLP availability is dependent on the degree of protein binding of the ... Table The intron/exon junctions of the human PNPO gene The nucleotide sequences at exon (uppercase letters) and intron (lowercase letters) junction are shown Exon and intron sizes are indicated in ... +136 of the start codon Such a CpG island is indicative of the presence of a promoter region and indicates a widespread expression Analysis of the 5¢-flanking human PNPO gene sequence using PROMOTORINSPECTOR...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
Ngày tải lên :
18/06/2014, 15:20
... [34] The reduced response of non-healing edge cells is not surprising, as the cells appear to retain their phenotype in vitro It is surprising, then, that GM-CSF stimulated migration of these cells ... assistance in preparation of the manuscript References The National Institute on Aging Cell Repository at Coriell is the first containing cells strains derived from chronic wounds By using the methodology ... http://www.translational-medicine.com/content/6/1/75 32 33 34 35 36 37 38 39 of chronic wounds: the role of beta-catenin and c-myc in the inhibition of epithelialization and wound healing Am J Pathol 2005,...
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báo cáo hóa học: " Soluble HLA measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary study" pdf
Ngày tải lên :
19/06/2014, 22:20
... Detection of lesions, compatible with MS, was made by visual inspection as was determination of the absence or the presence of contrast enhancing lesion Computer based software allowed comparison of ... subgrouping them into those with enhancing lesions vs those without enhancing lesions on brain MRI, with the understanding that contrast enhancement tends to reflect disease activity Comparison of ... differences The measurements of the saliva and the CSF HLA-I demonstrated the following: sHLA I was highly elevated in the CSF and saliva of only two patients with RRMS, during an exacerbation (mean...
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Báo cáo hóa học: " Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue" pdf
Ngày tải lên :
20/06/2014, 01:20
... production The effect of timing of TLR-ligand exposure on inhibition of HCMV infection production was next investigated using the concentration of each TLR-ligand that most effectively inhibited infection ... time of TLR stimulation on HCMV infection and IL-8 secretion in Foreskin Fibroblasts The effect of time of TLR stimulation on HCMV infection and IL-8 secretion in Foreskin Fibroblasts A Cell monlayers ... secretion and HCMV inhibition in HFF induced by TLR ligands IL-8 secretion and HCMV inhibition in HFF induced by TLR ligands HFF cells were cultured to 95% confluency and stimulated with the indicated...
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