origins of the c
A Quick Tour of the C++CLI Language Features
Ngày tải lên :
05/10/2013, 08:20
... }
};
You could compile the class unchanged in C+ +/CLI with the following command line:
cl /clr atom.cpp
and it would be a valid C+ +/CLI program. That’s because C+ +/CLI is a superset of C+ +, so any
C+ + ... static_cast (or dynamic_cast),
we use a casting construct that is introduced in C+ +/CLI, safe_cast. A safe cast is a cast in
which there is, if needed, a runtime check for validity. Actually, there ... implementation of RadioactiveAtom that
implements the IRadioactive interface.
Note the absence of the public keyword in the base class and interface list. Inheritance is
always public in C+ +/CLI, so there...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
Ngày tải lên :
06/03/2014, 11:20
... hypothe-
sized to be a result of the presence of an amino acid
that occludes the active site. This idea has been
proposed because of similarities in the structures of the
C- terminals of the related ... twice that of the recombinant core
tyrosinase, although the K
m
for both substrates is
almost identical. The increased level of activity is prob-
ably a result of the higher copper content of the ... 3).
Recombinant core tyrosinase
To further asses the functional importance of the
C- terminal extension, we created a shortened form of
the V. spinosum tyrosinase, using the presence of the
conserved...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
Ngày tải lên :
06/03/2014, 11:20
... the C signal
of the preceding amino acid, correlating the amide HN and
the Ca signals, correlating the amide HN and the Ca signal
of the preceding amino acid, correlating the amide NH with
the ... for the determination
of the structures of the conformers of the C- domain.
The dipolar couplings provided long-distance informa-
tion on the global folding of both conformers.
The structure of the ... of the protein.
Fig. S2. Plot of the number and distribution of the
NOEs versus the amino acid sequence that were used
in the structure calculation for the open and closed
conformers of the C- terminal...
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
Ngày tải lên :
07/03/2014, 12:20
... 1009
plants, the product (E)-cinnamic acid is hydroxylated
at the para-position by cinnamate-4-hydroxylase
(C4 H), in conjunction with NADPH:cytochrome P450
reductase (CPR). The coordinated reactions catalyzed
by ... basis of the
functional similarity of HAL and PAL, of the same
electrophilic prosthetic group at the active sites, and of
the sequence conservation over a large evolutionary
distance (mammals, bacteria, ... contained the Tyr110-loop, but in a con-
formation which separates the phenolic O-atom of
Tyr110 more than 17 A
˚
apart from the exocyclic
methylene C- atom of the MIO prosthetic group.
On the basis of...
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Báo cáo khoa học: by nucleotide affinity cleavage: a distinct nucleotide specificity of the C-terminal ATP-binding site potx
Ngày tải lên :
08/03/2014, 02:21
... panel).
In conclusion, the present studies provide the first
systematic and detailed characterization of the nucleotide
binding specificity of the N- and C- terminal nucleotide
binding sites of the 90-kDa ... C- terminal
preference (Fig. 2B). These experiments provided evidence
for the applicability of the nucleotide affinity cleavage
technique to study the specificity of the nucleotide binding
sites. Since GTP is a C- terminal-speci c ... after the occupancy of the
N-terminal site. Our present work demonstrates the appli-
cability of the oxidative nucleotide affinity cleavage in the
site-speci c characterization of nucleotide binding...
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![Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf](https://media.store123doc.com/images/document/14/rc/ty/medium_tyq1394220086.jpg)
Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf
Ngày tải lên :
08/03/2014, 02:21
... Na,Ca,Cb,Cc¢ atoms of the pyrrolidine cycle. v
1
and v
2
torsion angles correspond to the side chain of the prolinoamino acid and are
defined by Na,Ca,Cb,Cc and Ca,Cb,Cc,Cd(proR) atoms.
Ac-P
c
3
Leu-NHMe
w, ... b-substituent on the pyrrolidine cycle (Met side
chain) does not further affect the conformation of leucine.
In the case of Gly-Xaa dipeptides, the conformation effect
of the pyrrolidine cycle is much smaller ... and potency towards the NK-1 receptor. The
structural implications of these results on the bioactive
conformation of Leu10 and the C- terminal tripeptide of SP
are analyzed by NMR spectroscopy on...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
Ngày tải lên :
08/03/2014, 22:20
... the
human CD53 cDNA to mimic the CD53 ectopic expression
in carcinomas. In stable CD53-transfected cells it stimulated
Jun-dependent transcriptional activity. We conclude that
parts of the cell responses ... reintroduction of CD9 in the cell func tions
as a b rake [31]. In addition, CD53 deficiency has a clinical
phenotype similar to those of inherited defects of cell
adhesion molecules [32]. Because of the complex ... progression
through the cell cycle. The differe nces in the effects caused
by mAbs are due to their recognition of different epitopes
on the CD53 molecule. All of these data indicated that
tetraspanin...
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Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf
Ngày tải lên :
16/03/2014, 00:20
... mutant, the
forward primer 5¢-CGAATCCCTACCCCAAGATCTGAT
CGGGCCTGAAGCGTCGCCG-3¢ and the reverse primer
5¢-CGGCGACGCTTCAGGCCCGATCAGATCTTGGGG
TAGGGATTCG-3¢ were used. Briefly, the mutagenesis
was achieved ... 5¢-ACCCCAAGATCGACTGGGCGG TAAG
CGTCGCGCTGGGTGGAGGA-3¢ and 5¢-TCCTCCACC
CAGCGGCGACGCTTACCGCCCGAGTCGATCTTGG
GGT-3¢ were used as forward and reverse primers, respec-
tively. For construction of the Tr(delDSGL
559
) ... but the processing is
distinct from that of the ascomycete laccases, because
the C- terminus of C. cinereus laccase does not contain
the conserved ascomycete cleavage site [24].
The role of C- terminal...
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
Ngày tải lên :
17/03/2014, 11:20
... AAA CAG CAT CCC CTC ggc CAT CGG GGT CAA CGC-3¢ CfrI
5¢R62A 5¢-CTG CAT GTG CTG GTA GAG gcc TAC GAT GTA TTC CGC ACG-3¢ AatI
3¢R62A 5¢-CGT GCG GAA TAC ATC GTA ggc CTC TAC CAG CAC ATG CAG-3¢ AatI
5¢R67A ... ATA CGA TGT ATT Cgc gAC GTT GTT TAT CTA TGA
AAA GC-3¢
NruI
3¢R67A 5¢-GCT TTT CAT AGA GAA ACA ACG Tcg cGA ATA CAT CGT ATC TCT
CTA CC-3¢
NruI
5¢W202L 5¢-CAG GCC GCT CTC AAC TAc Tta AGC GAC TAT CTG ... GCC-3¢ A¯II
3¢W202L 5¢-GGC TTC CAG ATA GTC GCT taA gTA GTT GAG AGC GGC CTG-3¢ A¯II
5¢R284A 5¢-GGC TCT GTT GTA TCC GGA gct CCT ACA GAC ATC GTC GG-3¢ SacI
3¢R284A 5¢-CCG ACG ATG TCT GTA GGa gcT...
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