... Fe-proteins or other proteins.Results and DiscussionSite-directed mutagenesis ofthe target arginine of dinitrogenase reductaseAn indication for the covalent modification of onesubunit of dinitrogenase ... azinc-imidazole stain [37]; and (d) a copper stain [38], aswell as their impact onthe further processing of pro-teins by MS. In all staining methods, except for the conventional Coomassie stain, the pH ... catalyzes the ADP-ribosylationreaction in this b-proteobacterium on one subunit of the nitrogenase Fe-protein, leading to the inactivation of the enzyme. Thus, the results obtained inthe pres-ent...
... LPSs in increasing the rate of oxidation, suggesting a difference in binding of the two types of LPSs. The effect ofthe LPSs onthe rate of oxidation of Hb A0was much less than on cross-linked ... the binding of LPS to the hemoglobin. The effect ofthe LPS appeared to be largely on the initial fast phase ofthe oxidation reaction, suggest-ing modification ofthe heme pocket ofthe a chains. ... Comparison ofthe effects ofthe LPSs of rough S. minnesota 595(Re) and smooth S. minnesota, inthe absence and presence of EDTA, on the oxidation of a, a-cross-linked Hb. Themean±SDofthreeinde-pendent...
... publication, Integrative Medicine andthe Health ofthe Public: A Summary ofthe 2009 Summit, provides an account ofthe discussion and presentations ofthe two -and- a-half day summit in Washington, ... are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, andthe Institute of Medicine. The members ofthe committee responsible for the report were ... that, for the 40 to 50 percent ofthe population suffering from chronic conditions, the distinctions between prevention and treatment begin to break down since the interventions are much the same....
... within 6 months of confirmation of index case: MissedTwenty countries have had outbreaks since the start of 2010. The Programme has succeeded in stopping each of them within six months. Only one, ... ERADICATION INITIATIVEJune 2012 The Independent Monitoring Board was convened at the request ofthe World Health Assembly to monitor and guide the progress ofthe Global Polio Eradication Initiative’s ... detection, and should be far more widely used MissedReport ofthe Independent Monitoring Board ofthe Global Polio Eradication Initiative June 2012 Independent Monitoring Board ofthe Global...
... containing a carbohydrate-binding module areroutinely O-glycosylated inthe linker region between the catalytic domain andthe carbohydrate-bindingmodule. Regions 725–739 and 764–782 ofthe ... formatting of the ligand and macromolecule, as well as for setting the grid and docking parameters. Minor variations ofthe standardLamarckian genetic algorithm parameter settings were used: the ... wide range of proteins,including other GHs (clans E, F and J) [3] and hemo-pexin [20]. The CD spectrum ofthe latter protein [21]is very similar to the one observed here; therefore, the unusual...
... shown in bold.PCR conditions were as described previously. The frag-ments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR. The remaining portions of the polylinker introduced ... extracellular component and a cell surfaceassociated subunit containing the transmembraneregion andthe cytoplasmic tail. These two parts of the protein remain associated by noncovalent interac-tion, probably ... the role of this domain in the processing of membrane-tethered mucins. The mech-anism by which the heavily glycosylated portion of these molecules is released at epithelial surfaces withinthe...
... preventing the ‘spreading’ of competing chromatinstructures between iab-7 and iab-8. This assumption iscompatible with the location ofthe PTS. The discovery and characterization ofthe PTS in ... later than inthe case of the histone gene cluster [10]. This difference inthe tim-ing of somatic pairing perhaps reflects the differencebetween the complexities ofthe regulation ofthe twosystems: ... This ‘breathing’ ofthe paired state might berequired for the reorganization of intramolecular inter-actions andthe correction of an inappropriate loopingstructure in later stages of development.If...
... oered by a software for inbreeding control 4 120Meuwissen, T.H.E. Genetic conservation of small populations from theory to practice inthe Netherlands 5 121Windig, J.J. Management of genetic variability ... M.C. and Carolino, M.I.BLUESEL – an INTERREG France-Wallonie-Vlaanderen project aiming at the conservation andthe use ofthe genetic heritage ofthe dual-purpose Blue Breeds in Belgium and ... Caprita, R. The biochemical indices ofthe milk and meat in goats with and without digestive strongilate invasion 53 59Birgele, E., Keidane, D. and Ilgaža, A. The eect ofthe inclusion grape...
... extension; to improve the technical and economic conditions ofthe livestock sector; to promote the welfare of farm animals andthe conservation of the rural environment; to control and optimise the ... one ofthe worldwide most important scientic meetings of professionals working inthe area of animal production.One ofthe main objectives ofthe meeting is to focus on sustainability and eciency ... Hofmanova, B. and Vostry, L.Management of inbreeding rates and relatedness in pedigreed dogs inthe Netherlands 3 68Windig, J.J. and Oldenbroek, J.K.Conservation of genetic diversity within...
... resulting in a delay in strandinsertion during reaction with the target proteinase. On the other hand, the Triton X-100-induced substrate behaviourdid not seem to implicate the interactions ofthe ... results in an < 7-nm translocation of the proteinase from the position of its initial encounter with the RCL to the other pole ofthe serpin [7– 10]. The translocation results in distortion ofthe ... vitronectindid not delay latency transition of any ofthe variantsharbouring the K335A substitution. Onthe basis of the opposite effects ofthe K335A substitution inthe absence and presence of...
... than the true value, underlining the impact of quite asmall level of contamination on these results.Reassessment ofthe effects ofthe mutations on coenzyme specificity In view ofthe dramatically ... before the steady state is reached: the presence or absence of aburst provides information onthe position ofthe rate-limiting step along the reaction pathway, and the amplitude ofthe burst ... estimatedthat, under the conditions used, 97–98% ofthe con-taminant NAD+should be converted into NADH.These results strongly suggest that NAD+is the con-taminant affecting the course ofthe enzymatic...
... ubiquitin-like (UbL) domain, the XPC-bindingdomain, and two ubiquitin-associated domains (UBA1 and UBA2) [9,10]. The UbL domain has a high bind-ing affinity for polyubiquitin binding site 2 of the human ... beanother advantage of adapting to the larger bindingcounter part. The difference in flexibility ofthe N-terminal regions of XPCB–hHR23B and XPCB–hHR23A may be oneway to explain why these domains, ... regions, in spite ofthe presence of internal motion (si) and aremarkable exchange process (Rex). The NOE valuescombined with the presence of internal motion and the exchange terms in N-terminal...
... byinterfering with ATP binding and therefore by limiting the energy necessary for the unwinding, (b) inhibition of ATPhydrolysis or release of ADP by blocking opening or closing of domain 2, (c) inhibition ... inhibition of RNA (or DNA) substratebinding, (d) inhibition of unwinding by sterically blockinghelicase translocation or (e) inhibition of coupling of ATPhydrolysis to unwinding. The present ... apolarchains of CK2. The only polar interaction, mediated by apair of hydrogen-bonded water molecules, involves the N(1) of the TBBT triazole ring and two charged side-chains of the protein [43]....
... 2007; Lino-Neto et al. 2008a; Mancini et al. 2006, 2008), indicating another possible source of characters that may contribute to understanding the systematics of these insects. In insects, ... the insertions ofthe deferent ducts (dd) into the accessory gland (g). Note the difference between the epithelium ofthe deferent duct (dd), showing basal and spherical nuclei (arrow), andthe ... additional information inthe systematics of Hymenoptera (Schiff et al. 2001; Zama et al. 2007; Lino-Neto et al. 2008a). Variation inthe morphology ofthe reproductive system in males of...