negative regulators of nf k b activation and type i interferon pathways

Báo cáo y học: " Inhibitor of IκB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells" ppsx

Ngày tải lên : 12/08/2014, 12:20

... IKKα in the inhibition of IFN-α and IKKβ in the inhibition of TNF-α at the downstream of TLR7/9-MyD88 The other two IKK-related kinases, TANK-binding kinase (TBK1) and IKKτ (also called as IKKε), ... phosphorylation and nuclear translocation depend on IKKα, NF- B activation needs IKKβ IKKβ homodimer can compensate the function of heterodimer of IKKα and IKKβ in activating NF- B in the absence of IKKα ... pathogenic conditioned IFN-α production under in vitro experiments BAY11 inhibits inducible IFN-α production in vivo Finally, to weigh up the possibility of inhibiting type I IFN production through BAY11...

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Báo cáo y học: " Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes" ppsx

Ngày tải lên : 13/08/2014, 01:21

... vectors with wild -type or chimeric Ad5/35 fiber encoding murine type I IFN subtypes IFNa1, IFNa2, IFNa4, IFNa5, IFNa6, IFNa9 or IFNb The identities of the IFN subtypes were verified by sequencing and ... infection [4-9] While the antiviral functions of type I IFNs have been elucidated in detail, and IFN combination therapy is the standard of care in some viral infections like chronic hepatitis ... formation Enhanced antibody titers after co-administration of specific type I IFN subtypes Virus-specific antibodies have been shown to play an important role in vaccine protection against FV infection...

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Báo cáo y học: "κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocyte" ppt

Ngày tải lên : 09/08/2014, 01:23

... cytokines have to be considered, regarding their mode of action and their contribution to disease In conditions where individual cytokines had no significant effect, their combination resulted in ... criteria for the classification of rheumatoid arthritis Arthritis Rheum 1988, 31:315-324 Ziolkowska M., Kurowska M, Radzikowska A, Luszczykiewicz G, Wiland P, Dziewczopolski W, Filipowicz-Sosnowska ... Hata K, Andoh A, Shimada M, Fujino S, Bamba S, Araki Y, Okuno T, Fujiyama Y, Bamba T: IL-17 stimulates inflammatory responses via NF- kappaB and MAP kinase pathways in human colonic myofibroblasts...

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Báo cáo y học: "Early Inhibition of NF-κB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol)" pot

Ngày tải lên : 11/08/2014, 08:21

... Inhibition bark extract after five days intakeby plasma of five Inhibition of LPS-induced NF- B activation by plasma of five volunteers before and after five days intake of 200 mg maritime pine ... teers before and after matching plasma and MMP-9 of inhibition of LPS-induced NF- B of five volunCorrelation release by five days intake samples activation Correlation of inhibition of LPS-induced ... restricting NF- B activation by green tea polyphenols [25] The mechanism of ex vivo inhibition of NF- B nuclear translocation by plasma containing bioavailable active principles after Pycnogenol ingestion...

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Structure and rheology of mixtures of the protein b lactoglobulin and the polysaccharide k carrageenan

Ngày tải lên : 12/07/2015, 13:00

... biophysical studies because of its abundance and ease of isolation from milk Its biological function is not clear, but it is a member of the lipocalin family of proteins (Banaszak et al., 1994; ... protein rich domains, which explained the reduction of the turbidity, but the domains remained visible in microscopy In another study, microphase separation was observed during cooling a mixture of ... Optical rotatory dispersion and sedimentation in the study of association-dissociation: Bovine β-lactoglobulins near pH5 Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology,...

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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

Ngày tải lên : 19/02/2014, 16:20

... without Bsr gene Position of the probe (0.9 kb; )10.1 kb HindIII to )9.2 kb AccI fragment) for hybridization is indicated by a black rectangle Size and position of the HindIII fragment hybridizable ... ()7.8 kb to )6.0 kb; DHSs, )7.3 kb and )6.5 kb); region II, promoter and upstream region of Ig -b gene ()2.7 kb to +0.05 kb; DHSs, )2.1 kb, )1.0 kb, and kb); region III, in the ﬁrst intron of the Ig -b ... HindIII, and hybridized with a 0.9 kb probe for the Na channel gene; for regions II (Fig 3B) and III (Fig 3C), DNA was cut with EcoRI, and hybridized with Ig -b cDNA; for region IV (Fig 3D), SacI-digested...

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Báo cáo khoa học: Involvement of NF-jB subunit p65 and retinoic acid receptors, RARa and RXRa, in transcriptional regulation of the human GnRH II gene pot

Ngày tải lên : 16/03/2014, 10:20

... abolished by RAR antibody and RXR antibody, another speciﬁc Complex (Complex B) increased in intensity This may imply competition binding between RAR and ⁄ or RXR and Complex B on the hGII-Sil ... assay to identify which members of the RAR family bind to the Mut4 and Mut5 (hGII-Sil) region in the putative intronic silencing element in TE671 cells Synthetic oligonucleotides of hGII-Sil were ... hGII-Sil in TE671 cells According to the results of supershift assays, NF- jB p65 subunit antibody and RAR antibody abolished the formation of Complex A, indicating that the p65 subunit and members...

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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc

Ngày tải lên : 09/08/2014, 14:22

... autoantibody profile and TLR-dependent B- cell response in SLE mice genetically deficient in the IFNAR2 chain of the IFNAR Autoantibody profiling using autoantigen microarrays in combination with ... Schreiber RD, Sheehan K, Hilton DJ, Alexander WS, Hertzog PJ: Suppressor of cytokine signaling regulates the immune response to infection by a unique inhibition of type I interferon activity Nat Immunol ... chain exists in both transmembrane and soluble isoforms and is critical for ligand binding and signal transduction through the receptor [27,28] Negative regulators of IFN and other proinflammatory...

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Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf

Ngày tải lên : 12/08/2014, 14:20

... Chwieralski CE, Brule S van den, Röcken C, Reinhold D, Welte T, B hling F: Modulation of cytokine production and silica-induced lung fibrosis by inhibitors of aminopeptidase N and of dipeptidyl ... grade n Silicotic nodule grade Saline control Silica Silica +Lv-shCD36 Silica +Lv-shCD36-NC 8 8 I ~I I 8 8 I ~II I I ~II 8 8 II I II Page of (page number not for citation purposes) Respiratory ... of silicosis was induced by an intratracheal administration of silica dust, resulting in varying degrees of fibrotic silicosis [15] Following silica-induced lung injury, AMs were stimulated and...

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Báo cáo y học: "Prothrombin complex concentrate in surgical patients: retrospective evaluation of vitamin K antagonist reversal and treatment of severe bleeding" pdf

Ngày tải lên : 13/08/2014, 20:21

... coagulation inhibitors protein C and protein S [18] Beriplex P/N® is prepared using pasteurization and nanofiltration to facilitate viral inactivation and elimination [19] As PCCs are able to normalize ... six days One factor that will influence the speed of INR correction with PCCs is body temperature Hypothermia inhibits the synthesis of fibrinogen and the initiation phase of thrombin generation ... cardiocirculatory instability following activation of coagulation The dose of PCC administered was significantly higher in bleeding patients than in anticoagulation reversal patients This was probably because...

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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx

Ngày tải lên : 19/02/2014, 00:20

... maturation and distribution of the K6 50M mutant The kinase activity of FGFRs, including FGFR3 [37,38], is inhibited by SU5402, which binds to the kinases’ ATP-binding site [39] We therefore determined ... 21323–21326 Huang F, Kirkpatrick D, Jiang X, Gygi S & Sorkin A (2006) Differential regulation of EGF receptor internalisation and degradation by multiubiquitination within the kinase domain Mol Cell 21, ... membrane The E3-ubiquitin ligase c-Cbl is directly involved in the ubiquitylation of several RTKs [24–26,32] and may participate in the downregulation of FGFR1 via an indirect interaction with...

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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx

Ngày tải lên : 20/06/2014, 01:20

... activate TLRs and retinoic acid inducible gene I (RIG -I) signaling pathways leading to phosphorylation of interferon regulatory factor3 (IRF3) and IRF7 and stimulation of type I interferon (IFN) ... ability of RSV to induce expression and catalytic activity IKKε which blocks RSV-induced IRF3 phosphorylation, nuclear translocation and DNA-binding, and leading to inhibition of cytokine gene ... Sasaki T, Tashiro M, Kato A, Ozato K: Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression J Biol Chem 2008 Kunzi MS, Pitha...

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Báo cáo y học: "Interactions among type I and type II interferon, tumor necrosis factor, and -estradiol in the regulation of immune response-related gene expressions in systemic lupus erythematosus" potx

Ngày tải lên : 09/08/2014, 01:22

... effect of TNF on interferon- inducible gene expressions in peripheral blood mononuclear cells in vitro The expression of 15 IFI genes (IFIT1, IFIT3, IFIT5, IFI6, IFI16, IFI27, IFI30, IFI35, interferon- induced ... stimulation in vitro Meanwhile, IFI genes such as IFIT1, IFIT3, OAS1, ISG15 and IRF7, and IL8RA were upregulated in vivo, but IFIT1, IFIT3, OAS1 and IL8RA were downregulated, while ISG15 and IRF7 ... that blocking TNF signaling increases the production of IFN by plasmacytoid dendritic cells and induces an IFN signature in the blood of arthritis patients [35] This may be another mechanism...

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Báo cáo y học: " Interactions among type I and type II interferon, tumor necrosis factor, and -estradiol in the regulation of immune response-related gene expressions in systemic lupus erythematosus" pptx

Ngày tải lên : 09/08/2014, 13:22

... effect of TNF on interferon- inducible gene expressions in peripheral blood mononuclear cells in vitro The expression of 15 IFI genes (IFIT1, IFIT3, IFIT5, IFI6, IFI16, IFI27, IFI30, IFI35, interferon- induced ... stimulation in vitro Meanwhile, IFI genes such as IFIT1, IFIT3, OAS1, ISG15 and IRF7, and IL8RA were upregulated in vivo, but IFIT1, IFIT3, OAS1 and IL8RA were downregulated, while ISG15 and IRF7 ... that blocking TNF signaling increases the production of IFN by plasmacytoid dendritic cells and induces an IFN signature in the blood of arthritis patients [35] This may be another mechanism...

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DISCOVERY OF NOVEL REGULATORS OF TYPE i INTERFERON RESPONSE

Ngày tải lên : 04/10/2015, 16:03

... polyubiquitin chain then activates a complex consisting of TAK1, TAK1-binding protein (TAB1), TAB2, and TAB3 leading to the phosphorylation of I B kinase (IKK)-β and MAP kinase kinase This brings ... replication and the escalating of infection inside the host (Schoggins & Rice, 2011) 15 The ubiquitination mediated regulation of cell signalling The ubiquitin ligases and ubiquitination Ubiquitination ... motif RIP1 then undergoes K6 3-linked polyubiquitination and consequently results in NF B activation Beside NF- B activation, TRIF also mediates the expression of Type- I IFN by recruiting signalling...

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Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx

Ngày tải lên : 07/03/2014, 12:20

... Zamanian-Daryoush M, Mogensen TH, DiDonato JA & Williams BR (2000) NF- kappaB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF- kappaB-inducing kinase and IkappaB ... to virus infection in Atlantic salmon A role for NFjB in the activation of PR -I was further supported by two different inhibitor experiments First, dose-dependent inhibition of promoter activity ... activity by the NFjB inhibitor PDTC was shown (Fig 7) PDTC is a metal chelator with antioxidant properties which speciﬁcally inhibits NFjB-induced pathways [39] Secondly, partial inhibition of promoter...

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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

Ngày tải lên : 16/03/2014, 12:20

... accompanied by increased sensitivity of the enzyme to inhibition by agmatine and putrescine, the decarboxylated derivatives of arginine and ornithine, respectively (Fig 1) However, the most important ... ´ R, Uribe E & Lopez V (2004) Kinetic and site directed mutagenesis studies of Escherichia coli agmatinase A: role for Glu274 in binding and correct positioning of the substrate guanidinium group ... would explain the increased Km value for arginine inhibition and Ki value for ornithine inhibition of the N130D variant The large effect of the Asn130 ﬁ Asp mutation on the kcat value indicates that...

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Báo cáo y học: "Systemic lupus erythematosus and the type I interferon system" docx

Ngày tải lên : 09/08/2014, 01:21

... their activation of NIPCs/PDCs through the FcγRII could be blocked The actions of the IFN-α produced could furthermore be inhibited by neutralizing anti-IFN-α antibodies [79], antibodies blocking ... both for therapy and to maintain remissions in SLE patients This drug is known to inhibit IFN-α production by NIPCs/PDCs in vitro by the inhibition of endosomal acidification/maturation [23] The ... [48,62,64] In addition, TNF-α inhibited the action of these inducers [62]; this observation is interesting because it can explain why a blockade of TNF-α by antiTNF-α antibodies or soluble TNF-α receptors...

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Báo cáo y học: "Monocyte surface expression of Fcγ receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus" pptx

Ngày tải lên : 12/08/2014, 12:20

... pericarditis, and hematologic abnormalities In addition, the presence of anti-dsDNA and anti-Sm autoantibodies, but not antiphospholipid antibodies, was linked to increased CD64 expression (Table ... 0.05, Spearman's rank correlation; Figure 4b) The effects of SLE sera were inhibited by the addition of B1 8R, but not neutralizing antibodies to IL-12 or IFN-γ, indicating that IFN -I was the major ... neutrophils is a sensitive and specific marker for detection of infection in patients with rheumatoid arthritis J Rheumatol 2006, 33:2416-2424 Biesen R, Demir C, Barkhudarova F, Grun JR, Steinbrich-Zollner...

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Báo cáo y học: "Toll-like receptor 3 upregulation by type I interferon in healthy and scleroderma dermal fibroblasts" ppt

Ngày tải lên : 12/08/2014, 15:22

... stimulation of dermal fibroblasts Myofibroblasts have increased upregulation of TLR3 SSc skin biopsies have increased numbers of myofibroblasts [3] In vitro TGF -b induces the differentiation from fibroblasts ... Suzuki H, Takemura H, Yoshizaki K, Koishihara Y, Ohsugi Y, Okano A, Akiyama Y, Tojo T, Kishimoto T, Kashiwagi H: IL-6-anti-IL-6 autoantibody complexes with IL-6 activity in sera from some patients ... deposition in the bleomycin-induced skin fibrosis model [38] However, clinical trials of recombinant IFN-g or IFN-a in patients with SSc failed to show substantial clinical benefit [39-41] The lack...

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