mutated human embryonic stem cells for the study of human genetic disorders
Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... source of cells and the potential to derive the cell type of interest together with the possibility to enrich for genetic modifications during the dividing stem cell state 1.1.1 Human pluripotent stem ... by the integration of wild-type genes into the AAVS1 locus The use of AAVs was further simplified by the arrival of commercially available systems Following the hypothesis that homology of the...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... indicated the uniqueness of the human ES cells from the rest of the cells, cancerous or normal and that human EC cells shared a common core set of pluripotency genes with human ES cells In the ES/EC ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... 1974) The pioneering work in mouse EC cells paved the way to the derivation of pluripotent cells from the ICM of mouse blastocysts, termed embryonic stem (ES) cells, under culture condition of fibroblast...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
... PD, the application of animal models for studying PD, and possible therapeutic application of stem cells in the treatment of PD will be reviewed Based on this review, the scope of the present study ... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... after the initiating agent has disappeared The MPTP model of PD has been invaluable in the studying of the mechanisms of PD pathogenesis, for example, the mechanisms of microglial activation and their...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... characterized by the breakdown of the joint’s cartilage Cartilage is the part of the joint that cushions the ends of the bones and allows easy movement of joints The breakdown of cartilage causes the bones ... 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from the inner cell mass of the blastocyst-stage embryo (Thomson et al., 1998) Human ESCs have the capacity ... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage...
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Human embryonic stem cells for genotoxicity testing
... established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been validated ... Determination of Genotoxicity using Mitomycin C…………………………… 26 iii CHAPTER 4 RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human ... evidenced by the lower levels of chromosomal aberration in hESCs compared to IMR-90 The resistance of hESCs to genotoxic damage highlights the possibility of efficient repair mechanisms in these cells...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... FLSs [32] Therefore, we further investigated the effect of UC-MSCs on the invasive behavior of FLSs by the Cell Migration/Invasion Assay, and the MMP9 secretion of FLSs As a result, the invasive ... [44] Therefore, reduction of MCP-1 could partly explain the absence of inflammatory infiltrates in the synovium of mice treated with human UC-MSCs Moreover, UC-MSCs increased the levels of the ... in the joint of the CIA mice The short-term presence of UC-MSCs in the spleen suggests that the therapeutic effect of UC-MSCs does not rely on the capacity to engraft and survive long-term in the...
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Báo cáo y học: " The utilization of humanized mouse models for the study of human retroviral infections" docx
... tissues, therefore allowing the engraftment of human cells and/or tissues This model represents the landmark experiment that sparked further development of humanized mice for the study of human ... Additionally, the presence of the fetal thy/ liv organ allows for innate maturation of human CD4+ and CD8+ T cells in the context of the animal's own immune system In general, the proliferation of human cells ... highlight of the noteworthy events of humanized mouse model system development over the past 30 years The bottom half of the timeline denotes the emergence of key humanized mouse models The top half of...
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Báo cáo y học: "Use of a multi-virus array for the study of human viral and retroviral pathogens: gene expression studies and ChIP-chip analysis" pot
... listing the identity of the PCR primers used for the production of the amplicons, their exact coordinates on the genomes, the sizes of the probes generated and the success of the amplification Also ... in some of the expression studies AP performed many of the expression studies and the ChIP-chip analysis VL performed the ChIP-chip analysis CD participated in the design and printing of the array ... to amplicons of other viruses, only two (proviral LTR and nef) of the 11 spotted regions of the HIV-1 genome hybridized to the DNA sample The G/C content of the entire coding region of HIV is 42%...
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Tài liệu Towards a framework for the study of the neural correlates of aesthetic preference pdf
... consequences for the comparison of the results of this study with the others Jacobsen et al (2006) acknowledged this in the first paragraph of their paper when referring to the other set of studies: ... none of these studies aimed at identifying the network of aesthetic judgment per se” (Jacobsen et al., 2006, p 276) In sum, while the question of the brain correlates of judging the beauty of images ... of the emotional response associated with aesthetic preference have been captured by two of the reviewed studies: the representation of reward value and the awareness of the emotional state The...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... with many human adult stem cells The application of stem cell research to therapies for human disease will require much more knowledge about the biological properties of all types of stem cells Although ... or the brain, in the adult body They can renew themselves in the body, making identical copies of themselves for the lifetime of the organism, or become specialized to yield the cell types of the ... identical to the donor, promises such an advantage Other options for this purpose include genetic manipulation of the stem cells and the development of a very large bank of embryonic stem cell lines...
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Report Of The Committee On Proposal Evaluation For Allocation Of Supercomputing Time For The Study Of Molecular Dynamics pptx
... letter describes the work and transmits the final report of the Committee on Proposal Evaluation for Allocation of Supercomputing Time for the Study of Molecular Dynamics, Second Round The committee ... committee of experts to evaluate the proposals submitted in response to the aforementioned RFP The committee of 17 was chaired by Dr Robert L Jernigan, Director of the Baker Center for Bioinformatics ... an Anton system housed at the Pittsburgh Supercomputing Center (PSC), based on the advice of a previous National Research Council committee convened in the fall of 2010 The success of the program...
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Báo cáo khoa học: Data-driven docking for the study of biomolecular complexes pptx
... for the deleted atoms [13] Another point is that one should, in principle, always check whether the mutants not affect the 3D structure of the free components themselves, i.e whether or not the ... assessment of which residues of the labeled molecule are perturbed by the formation of the complex One then repeats this procedure with the second molecule labeled Under the assumption that the perturbed ... the residues that are on the surface of the partner proteins The general idea then is that mutation of an interface residue will influence the interaction, whereas for non-interface residues the...
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báo cáo hóa học: " Muscle-driven forward dynamic simulations for the study of normal and pathological gait" potx
... regarding the role of the plantarflexors in propulsion, but their study examined the accelerations of the hip rather than the trunk COM [40] Dynamic simulation has also been used to investigate the ... assess the effects of using generic joint models • Means for validation The results of dynamic simulations are likely to be sensitive to the degree of complexity in the formulation of the model ... modeling of contact between the feet and the ground; modeling of contact within the joints; the method used to integrate the equations of motion Also important is the scheme used to arrive at the...
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A FUNCTIONAL-ANALYTIC METHOD FOR THE STUDY OF DIFFERENCE EQUATIONS EUGENIA N. PETROPOULOU AND potx
... outline of the functional-analytic method that we will present in details in Section (For a sketch of the main ideas used in the proofs of our main results, see the beginning of Section 3.) By use of ... fixed point theorems) from the wealth of operator theory, in order to assure the existence of a unique solution of the operator equation in H or H1 In the case of linear equations, we use the following ... equations of three and four variables The aim of this paper is to present the generalization of this functional-analytic method for the study of linear and nonlinear partial difference equations of p...
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Báo cáo khoa học: " Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection" ppsx
... 4B shows that the majority of Huh7 cells were positive for the HCV E2 glycoprotein and that the entire aggregate was permissive for HCV infection rather than just the cells at the periphery, ... 10 11 12 13 The authors declare that they have no competing interests Authors' contributions BS and VT participated in the design of the study, performed the experiments and drafted the manuscript ... reported using the robust 2-D Huh7 system [2,46] To determine the percentage of cells expressing HCV proteins, indirect immunofluorescence analysis of infected 3-D Huh7 aggregates was performed Fig...
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Báo cáo khoa học: "Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection" pdf
... 4B shows that the majority of Huh7 cells were positive for the HCV E2 glycoprotein and that the entire aggregate was permissive for HCV infection rather than just the cells at the periphery, ... 10 11 12 13 The authors declare that they have no competing interests Authors' contributions BS and VT participated in the design of the study, performed the experiments and drafted the manuscript ... reported using the robust 2-D Huh7 system [2,46] To determine the percentage of cells expressing HCV proteins, indirect immunofluorescence analysis of infected 3-D Huh7 aggregates was performed Fig...
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Development of a fluorescence correlation spectroscopy method for the study of biomolecular interactions
... by the law of mass action: ratio of the concentrations of the free receptor [R] and the free ligand [L], to the concentration of the complex [RL] The concentrations of the free components are then ... easily distinguishes the products from the free reactants via the amplitude of the CCF, as compared to the weak dependence of the ACF with the mass of the complexes Assuming that there is no cross-talk ... is the viscosity of the solvent, and r is the radius of the sphere r is related to the mass of a sphere given by the product of the density ρ and volume V M = ρV = ρ πr3 (2.21) 17 Chapter Theory...
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An asthma allergen specific animal model for the study of responses to dust mite allergen induced asthma
... orchestrator of immune responses These cells produce a variety of cytokines that affect the function of the other cells in the immune system, both in the adaptive and innate immune components of the immune ... IL-13) These mediators can profoundly affect the permeability of the vascular endothelium, allowing the migration of circulating immune cells to migrate through the endothelium into the site of inflammation ... infections The role of the innate immune system of the lung is not only to protect the host from respiratory pathogens but also to maintain homeostasis The innate immune system is the first component of...
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Inverse modeling for the study of 2d doping profile of submicron transistor using process and device simulation
... is the range of the amorphous Pearson profile Rpb is the range of the channeled Pearson profile σ a is the standard deviation of the amorphous Pearson profile σ b is the standard deviation of the ... channeled Pearson profile γ a is the skewness of the amorphous Pearson profile γ b is the skewness of the channeled Pearson profile β a is the kurtosis of the amorphous Pearson profile - 28 - b β ... amorphous Pearson profiles, respectively, ratio is the ratio of the dose of the amorphous profile to the total dose and u is the coordinate along the depth A final profile will take the shape as illustrated...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... is the establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... 2008 The Authors Journal compilation ª 2008 FEBS 1625 Epigenetic study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells...
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