... (GCGCTGTCGACCATGGAGGACTT
TCTGCTCT CATTGAATTCCTCAAGTGCTTGCTAGC
CATG). The forward (5Â) primer contained a SalI site,
whereas the reverse (3Â) primer contained an EcoRI site. The
amplied products ... GGTGGTCATATGGAGG
ACTTTCTRCTCT ⁄ CACTTGCCATTGCTTTTATCA and
ligated into SmaI site of pUC 18 vector.
The E. coli pGEX–mTSSK3 or pGEX–hTSSK3 plasmids
were constructed using pGEX)4T-2, which expresses ... and Biophysics, Polish Academy of Sciences, Warsaw, Poland
3 Department of Physiological Chemistry and Center for Biomedical Genetics, University Medical Center Utrecht, the Netherlands
Phosphorylation...
... surfaces such as flows over stepped chute,
cascade, spillway, etc and the alike. In such cases, these models can not reproduce the
effects of the bottom topography (e.g., centrifugal force due ... nonorthogonal curvilinear coordinates for prediction of flow pattern in
meandering channel with 60
o
and 90
o
bend, and also with compound meandering channel.
In this study, the Cartesian velocity components ... or /and with an existing empirical
formula to show the applicability and effectiveness of the model.
1.4 Scope of Study
The manuscript consists of eight chapters including the Introduction and...
... experiments.
2. Create the project exp7a using CCS. Add the command file exp7.cmd, the
functions epx7a .c, fft_a .c, andibit_rev .c, and the header file
icomplex.h from the software package into the project.
340
FAST ... scaling
technique achieves much better accuracy since we may scale less often than the uncon-
ditional scaling method. However, this conditional scaling method increases software
complexity and ... Experiment 7C:
1. Create the project epx 7c and include the files exp7.cmd, exp 7c. c, w_table .c,
fft.asm, and bit_rev.asm from the software package into the project.
2. Build and view the IFFT results by...
... complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin
3-glucoside, sections Cand D).
A Michaelis–Menten constants of BSP electrogenic uptake (K
M
, lM)
Carnation Liver
5.28 ± ... Cocolo
1
, Enrico Braidot
2
, Elisa Petrussa
2
, Carlo Peresson
2
,
Nevenka Medic
1
, Francesco Macri
2
and Angelo Vianello
2
1 Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Universita
`
di ... known to
randomly revert by freezing and thawing the vesicle
suspension. Because as many as three cycles of freezing
and thawing did not decrease the speci c activity of
BSP electrogenic uptake,...
... metabolism of MDs in F. succinogenes. These
Table 1. Production of succinate and acetate by Fibrobacter succin-
ogenes grown on various carbon sources. CB, cellobiose; Glc, glu-
cose; M2, maltose; M3, ... the culture media after 48 h. Average standard deviations were
± 0.7 and 0.2 for acetate and succinate, respectively.
Concentration ⁄ m
M Concentration ⁄ mM
Sugar Succinate Acetate Sugar Succinate ... 45
37.5
30
22.5
15
7.5
0
45
37.5
30
22.5
15
7.5
0
45
37.5
30
22.5
15
7.5
0
60
52.5
45
37.5
30
22.5
15
7.5
0
1.5
1.25
1
0.75
0.5
0.25
concentration (mM)concentration (mM)
concentration (mM)concentration (mM)
concentration (mM) concentration (mM) concentration (mM) concentration (mM)
0
01020
time (min)
S
A
B
C
D
S
S
SE
E
E
E
time...
... first application is to predict
function tags of an input syntactic parse tree; the sec-
ond one is to predict Chinese empty elements; and
the third one is to predict whether a syntactic con-
stituent ... the lexical features and it helps quite a
bit by improving the accuracy by 0.64%. When all
features are used, the system reached an accuracy of
97.34%.
From these results, we can conclude that, ... prediction and recov-
ery with unlexicalized PCFGs and slash features. In
Proceedings of the 21st International Conference on
Computational Linguistics and 44th Annual Meet-
ing of the Association...
... of
169
Yb complexes in normal and
tumour cells: influence of ligand and metabolic cell activity and
stability of cellular association. Nucl. Med. Biol. 24, 349–355.
6. Evans, C. H. (1990) Biochemistry ... the cells after incubation with FITC-Yb
2
-Tf by
flow cytometric technique based on the fluorescence excited
at 488 nm. As shown in Fig. 7A, the mean channel
fluorescence intensity (
FF), which ... mean channel fluorescence
intensity (
FF), which was obtained by integrating the
intensity per cell from 1 · 10
4
cells for each sample.
Laser scanning confocal microscopy
Entry of FITC-Yb-Tf...
... manufactured in commercial
quantities.
Commodity chemicals (e.g., polymer-grade acrylamide) and specialty chemicals can be
produced using biotech applications. Traditional chemical synthesis ... agricultural crops, such as corn, can be used in place of petroleum to produce chemicals.
The crop’s sugar can be fermented to acid, which can be then used as an intermediate to produce
other chemical ... develop crops with specific beneficial traits and reduce undesirable traits (10).
Traditional biotechnology such as cross-pollination in corn produces numerous, non-selective
changes. Genetic modifications...
... Jr., Homcy CJ, Vatner SF. One
hour of myocardial ischemia in conscious dogs increases beta-adrenergic receptors, but
decreases adenylate cyclase activity. Journal of Molecular and Cellular Cardiology
(1988). ... myocardial cell death due to prolonged
ischemia. Cell death is categorized pathologically by coagulation necrosis and/ or contraction
band necrosis, which usually evolves through oncosis, but can ... In contraction band necrosis and
colliquative myocytolysis, healing is thought to occur by fibroblastic proliferation, without the
usual sequence of changes that occurs with coagulation necrosis....
... 5’-AATCCATCTTGCTCCAA‐
CAC-3’ Rev- 5’ATCTCTTTTTCCGTCATCGTC-3’). PCR conditions were: Initial denatura‐
tion at 95 C for 15mins followed by 35 cycles (95 C for 1 min, 60 C for 1 min, 72 C for ... (Days)
pUb
pCMV
pGL3
Average Luminescence
p/sec/cm^2/sr/Gene Copy
Average Luminescence
p/sec/cm^2/sr/Gene Copy
Average Luminescence
p/sec/cm^2/sr/Gene Copy
pCMV-luc
Liver & Muscle
pUb-luc
Liver ... the landmark publications in the late 1980s [80]. Cationic lipids can condense nu‐
cleic acids into cationic particles when the components are mixed together. This cationic
lipid/nucleic acid complex...
... Detailed book review, subject could also be French Architecture
Richard A. Etlin, Symbolic Space: French Enlightenment Architecture andIts Legacy. Chicago:
The University of Chicago Press, 1994; xxv ...
Politics, science, social organization, and religion, all have spaces unique to their own functional
character. Beyond a functional character, however, there exists a deeper, more spiritual
connection ... that affect the viewer and the
user." Architecture can establish a meaning and a feeling; it will affect man's mood. The mood is
created and controlled by an architect's use and design...
... findings suggest that
APC protects hepatic nonparenchymal cells from
thrombin-induced inflammation, and that plasma PCI
in hPCI-Tg mice inhibits the cytoprotective action of
APC and may also inhibit ... PCI in hPCI-Tg mice inhibits the
cytoprotective activity of APC
The anticoagulant protease APC has marked cytopro-
tective and anti-inflammatory activities [31,32], and it
is generated from its ... representation of molecular homology between APC–PCI (left) and HGFA–PCI (right). APC (green), HGFA (red–orange) and
PCI (gray) are shown as ribbon models. In each complex, acidic and basic amino acids are...