lc hrms for the analysis of brs and their glucosides

báo cáo khoa học: " Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana" doc

Ngày tải lên : 11/08/2014, 11:21

... 48 and 96 hrs after addition of BL (1 μg/ml) Liquid chromatography high-resolution mass spectrometry (LC- HRMS) for the analysis of BRs and their glucosides A LC- HRMS method was developed for the ... in planta Kinetics of BL-glucoside catabolism in UGT73C5oe and UGT73C6oe plants To determine the kinetics of formation of the putative BR-MalGlc and BR-diGlc, the samples of the time- Husar et ... factor as for BR-23Glc The results are illustrated in Figure and show levels of BL-MalGlc and BL-diGlc in nmol/g Fw, as compared to BL and BL-23Glc amounts in seedlings of Col-0, UGT73C5oe and UGT73C6oe...

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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 1

Ngày tải lên : 14/09/2015, 10:36

... before sample injection to reduce the length of the concentrated sample zone and increase the peak height by slowing down the forward movement of the neutral sample associated with β-CD-NH2 and ... β-CD-NH2 and the backward movement of the neutral sample partitioned in the micelles of sodium dodecyl sulfate (SDS) Stability of the inclusion complexes formed between the carrier and the solute ... studies of many groups of compounds The improvement of sensitivity and the miniaturization of CE technique are two major trends of capillary electrophoresis which have gain more and more attention...

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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 2

Ngày tải lên : 14/09/2015, 10:37

... than the EOF as their velocity direction is the same as that of the EOF, so very rapid analysis times are therefore achieved with positively charged compounds The neutral compounds move at the ... presents one of the simplest and most effective means of influencing the EOF of the separation system Normally the EOF increases with decreasing buffer concentration and enables analysis of highly ... So the viscous drag of the solvent and the charge and size of the solute thus control the migration of a species in an applied electric field The separation efficiency, normally expressed as the...

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Novel methods (sonochemistry, microwave dielectric heating, sonoelectrochemistry and RAPET for the fabrication of nanomaterials and their applications

Ngày tải lên : 11/06/2014, 12:29

... used in our laboratory for the last four years We will show that the RAPET of silanes is yielding the smallest reported SiC particles having the highest surface area The RAPET of empty Coca Cola ... ability to reduce metals for which there is no reducing agent that can it For example, preparing metallic Mg nanoparticles .The last technique was named by us RAPET It stands for Reaction under Autogenic ... The main parameters that determine the particle size in sonoelectrochemistry are temperature, sonication intensity, and the electric pulse width Its main synthetic advantage is the ability...

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Báo cáo hóa học: " Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies" docx

Ngày tải lên : 20/06/2014, 02:20

... of each sample The numbers for the base positions that are reported here are the bases compared to the positions of the full length genome of HCV-N [44] Control HCV sequences for strains 1a and ... transmissions, and retransmissions of HCV JGP performed the clinical work, recruitment of patients, and procurement of specimens DR, MOA, RSB, DB, and NC performed the molecular work Acknowledgements The ... Samples from sera and isolates were cloned and sequenced The dates of transmission indicate the date that HCV was added to the cells For the T1 through T7 isolates, the exact date of transmission...

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Báo cáo lâm nghiệp: "of morphological characters and molecular markers for the analysis of hybridization in sessile and pedunculate oak" pps

Ngày tải lên : 08/08/2014, 18:21

... means of the comparison of the RAPD marker frequencies of a subsampling of these progenies and of the adult population Analysis of the morphological characters MATERIALS AND METHODS Sampling The ... Morphological analysis In the second case of the figure, the heterozygote deficit (f) must be considered The frequency of the phenotype A+ is the sum of the frequency of the genotype P : +/+ All of the ... situated on the side of the species in the majority represented in the neighbourhood, the sessile oak The comparison of the mean values of the groups on the first axis of DFAa by means of the F-test...

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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps

Ngày tải lên : 14/08/2014, 17:22

... with the technical aspects of data import and management, and refer the interested reader to the documentation of the software package prada for a thorough discussion of these [19] Selection of ... cleaning up of the data, removal of systematic biases and drifts (a process often referred to as 'normalization'), and transformation to a format and scale that is suitable for the following analysis ... (SSC), and they measure cell size and cell granularity (the amount of light-impermeable structures within the cell) One of the fluorescence channels monitors emission from the YFP tag of the overexpressed...

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Using biological networks and gene expression profiles for the analysis of diseases

Ngày tải lên : 09/09/2015, 10:15

... this case, the variances of the classes are pooled to compute the standard error, s2 and s2 denote the variance of the two classes respectively, N1 and N2 denote the sample size of the two classes ... s2 denote the variance of the two classes respectively, N1 and N2 denote the sample size of the two classes respectively Welch provides an approximation for the degrees of freedom for this test, ... Tk be the ordering of k genes sorted in increasing order of the modiﬁed t-statistic The permutation test randomly swaps the class labels of the original data, preserving the proportions of the...

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Capillary and microchip electrophoresis for the analysis of small biomolecules

Ngày tải lên : 02/10/2015, 12:56

... unsuitable for mass production because of the inherent serial nature of the system36 With the numerous benefits and the wide variety of substrates and techniques available for microfabrication of microchips, ... it and thereby generating the EOF The strength of this EOF is determined by Equation 1.2 below: μEOF = єζ/4πη (1.2) Where є refers to the dielectric constant of the buffer, ζ is the ... potential and η is the viscosity of the buffer The buffer parameters are affected by the composition of the buffer used, its pH as well as the type of organic additives introduced For instance, when the...

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Intrathecal baclofen for the control of spinal and supraspinal spasticity

Ngày tải lên : 01/11/2013, 10:20

... implanted for the relief of severe lower limb spasticity and ﬂexor spasms The goals then are improved comfort and wheelchair posture, Intrathecal baclofen for the control of spinal and supraspinal ... the process of consideration for ITB Patients may hear about ITB and discuss the question with their therapists rather than their doctors The charting of range and function before and after a ... cord than in the brain because of the characteristics of the circulation of the CSF from the ventricles to the spinal subarachnoid space The effective distribution volume of intrathecally administered...

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The analysis of theme and rheme in little house on the prairie = sự phân tích về đề ngữ và thuyết ngữ, thông qua truyện ngôi nhà nhỏ trên thảo nguyên

Ngày tải lên : 22/12/2013, 12:59

... that of the Interrogatives is the Interrogative word and that of the Imperatives is the Imperative form of the verb And Rheme is the remainder of the message, and also the part in which the Theme ... Interpersonal Themes, on the other hand, reveal something of the attitude of the speaker or the writer And Textual Theme is the link between the clause contains that Theme and the rest of the discourse ... Interpersonal Themes, and Textual Themes -Theme and Mood 3.1.1 Unmarked Themes and Marked Themes The Themes are often identified as the part chosen to be the departure point for a clause and in different...

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Tài liệu TREES OF THE NORTHERN UNITED STATES THEIR STUDY, DESCRIPTION AND DETERMINATION FOR THE USE OF SCHOOLS AND PRIVATE STUDENTS doc

Ngày tải lên : 13/02/2014, 12:20

... The last of the four kinds of parts is found in the center of the flower, and is called the pistil It is this part which forms the fruit and incloses the seed The stamens and the pistil are the ... plants themselves This chapter therefore will be devoted mainly to the words needed for leaf description, together with their application The Leaf.—In the axil of the whole leaf the bud forms for the ... little study of the roots of trees is practicable, some knowledge of their forms, varieties, and parts is important The great office of the roots of all plants is the taking in of food from the soil...

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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

Ngày tải lên : 14/02/2014, 22:20

... assessment of the trajectory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions ... The rate of net starch synthesis (rCO2 !Starch )was determined by interpolation of measured starch contents (unit: C6) and calculation of the ﬁrst derivative of this function The ﬂux rate of ... invertase activity The control of each assay was boiled for after addition of enzyme extract Reactions were incubated for 60 at 30 and °C, and stopped by boiling for min; the concentration of glucose...

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Tài liệu The Role of BCG Vaccine in the Prevention and Control of Tuberculosis in the United States: A Joint Statement by the Advisory Council for the Elimination of Tuberculosis and the Advisory Committee on Immunization Practices docx

Ngày tải lên : 15/02/2014, 13:20

... Centers for Disease Control and Prevention The role of BCG vaccine in the prevention and control of tuberculosis in the United States: a joint statement by the Advisory Council for the Elimination of ... RR-4 MMWR The Role of BCG Vaccine in the Prevention and Control of Tuberculosis in the United States A Joint Statement by the Advisory Council for the Elimination of Tuberculosis and the Advisory ... re-evaluation of the role of BCG vaccination in the prevention and control of TB This updated report is being issued by CDC, the Advisory Committee for the Elimination of Tuberculosis, and the Advisory...

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Tài liệu Multiple Vulnerabilities qualitave data for the stydy of orpharns and vulnerable children in South Africa ppt

Ngày tải lên : 19/02/2014, 00:20

... being done, found that the loss of either parent put strain on the child, as the loss of the mother often means loss of the direct carer, while the loss of the father puts the household in a difficult ... particular form of loss of a parent for a period of time while they go in search of income Orphans and deserted children are very dependent on the availability and quality of replacement carers for their ... communities and feel they have a better understanding of the most needy people within their areas They work together with DSD and the community to determine the criteria for receiving grants and also...

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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc

Ngày tải lên : 19/02/2014, 12:20

... activation states of the nAChR At the Table Comparison of a common motif in loop II of a4/7-conotoxins and their activity on oocyte-expressed a7 and a3b2 nAChRs The length/ hydrophobicity of the amino ... because of the impossibility of direct electrophysiological recordings and their incomplete pharmacological characterization Furthermore, the distance of the projection areas from the cell bodies and ... genomic DNA from other cone snail tissues The analysis of the DNA of different Conus species has already revealed a large number of a-conotoxin sequences [45] and the identiﬁcation of further speciﬁc...

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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx

Ngày tải lên : 05/03/2014, 21:20

... and, in the case of the sample uptake tubing, care must also be taken to avoid contamination of the sample through contact with the tubing material For routine analysis of organic solvents, the ... analysis of the organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some specific features and ... to the plasma, the plasma environment becomes considerably more reactive, and so the use of platinum-tipped interface cones instead of the standard nickel cones is recommended Optimization of the...

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Clinical Practice Guidelines for the Management of Overweight and Obesity in Adults ppt

Ngày tải lên : 08/03/2014, 14:20

... about the lack of evidence for the use of alternative, over -the- counter weight-loss medications and, in some cases, the possible dangers of their use xxviii Clinical Practice Guidelines for the ... Behavioural therapy can increase the effectiveness of other weight-loss treatments, and the duration of the therapy influences the ability to maintain the weight loss No single behavioural therapy ... strategic plan for the prevention of overweight and obesity The primary outcomes of that plan were the goals to ‘prevent further weight gain in adults and eventually reduce the proportion of the adult...

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A pharmacopoeia including the outlines of materia medica and therapeutics for the use of practitioners and students of veterinary medicine pdf

Ngày tải lên : 23/03/2014, 00:20

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SCOP: A Structural Classiﬁcation of Proteins Database for the Investigation of Sequences and Structures ppt

Ngày tải lên : 23/03/2014, 12:20

... Most of the folds are assigned to one of the ﬁve structural classes on the basis of the secondary structures of which they composed: (1) all alpha (for proteins whose structure is essentially formed ... Mosaic and the world wide web: global hypermedia protocols for the Internet Science, 265, 895–901 Sternberg, M J E & Thornton, J M (1976) On the conformation of proteins: the handedness of the b-strand–a-helix–b-strand ... Figure In scop, the unit of classiﬁcation is usually the protein domain Small proteins, and most of those of medium size, have a single domain and are, therefore, treated as a whole The domains in...

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