kinases transcription factors and cytokine expression in bmmcs
Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf
Ngày tải lên :
12/08/2014, 14:20
... positive (n = 4) and TST negative (n = 7) subjects TLR ligands induce production of pro-inflammatory cytokines by bronchoalveolar cells and monocytes To assess the cytokine- inducing functional ... variety of factors, including type and concentrations of cytokines and of additional proteins such as surfactant protein A [39] We also assessed the effects of TLR-specific ligands on the expression ... cell-type-specific ligandmediated regulation of the expression of TLR2 and TLR4 It was beyond the scope of this study to assess in detail whether ligand-binding alone, and/ or cytokine release in the cellular...
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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
Ngày tải lên :
09/08/2014, 01:23
... cells and in cell lines [9] In the RA synovium, p38 MAPK is predominantly activated in endothelial cells and in the lining layer [10] Inhibition of p38 MAPK therefore could provide an interesting ... mRNA expression and protein production, so inhibition is already apparent at the level of transcription R389 p38 MAPK activity in RA is found predominantly in the synovial lining layer and in endothelial ... cells in the synovium [10] The lining layer consists mainly of fibroblasts and macrophages, both important players in the process of inflammation by the production of cytokines and degrading enzymes...
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Báo cáo khoa học: "Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages" pot
Ngày tải lên :
07/08/2014, 15:20
... against the stimulation times of the kinetics of porcine cytokine mRNA expression in alveolar macrophages In this study, we examined seven porcine cytokine expression levels over a time course in ... blotting and an RT-PCR In conclusion, the results obtained in this study indicate that each porcine cytokine has its own expression pattern in alveolar macrophages depending upon the stimulator and ... study, the expression level of each cytokine gene was normalized against that of the cyclophilin A gene The final expression of each cytokine gene was determined by subtracting the expression...
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Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
Ngày tải lên :
13/08/2014, 18:22
... performed detecting organ-associated cytokine expression at the protein level In this context, it is well known that the release of cytokines is repressed in certain stages after trauma and sepsis ... differences in protein level after DHEA administration are caused by changes in cytokine transcription activity Moreover, we investigated mRNA expression levels in two organ compartments (liver and lung) ... be interfered by IGF-I, and that a variety of cytokines and growth factors play a role in the modulation of hormone secretion [37,38] This could result in timedependently varying reactions and...
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báo cáo khoa học: " Increased IL-10 mRNA expression in tumorassociated macrophage correlated with late stage of lung cancer" pps
Ngày tải lên :
10/08/2014, 10:21
... expression in macrophage, A, IL-10 staining in macrophage (strong positivity); B, CD68 staining C-D, Cathepsin B expression in macrophage; C, cathepsin B staining in macrophage (most cells were moderate ... (Figure 2B) The mRNA expression levels of IL-10, cathepsin B and cathepsin S in TAMs The mRNA expression levels of IL-10, cathepsin B and cathepsin S in TAMs were analyzed using QRT-PCR, compared ... best time point for analyzing the expression level of IL-10, cathepsin B and cathepsin S, a time course study was done After adhere to plastic for 20 min, 40 min, 60 and 90 min, the expression...
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Retrovirology Research BioMed Central Open Access APOBEC3G mRNA expression in exposed ppt
Ngày tải lên :
12/08/2014, 23:20
... hA3G by inducing its ubiquitination and degradation through the Vif protein [5-7] In vivo, the significance of hA3G-induced hypermutation as a protecting agent of clinical pathogenesis and HIV ... which include interaction with the HIV nucleocapsid with subsequent inhibition of tRNALys3 annealing to viral RNA, and DNA strand transfer during reverse transcription [2,3] Recently, studies in ... the expression levels varied with the decrease in viremia of their partners, or remained unchanged The findings would indicate either induced or constitutive hA3G expression, respectively In parallel,...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
Ngày tải lên :
21/02/2014, 15:20
... The efficacy of infection for varying viral loads was determined by staining for b-Gal (data not shown) Metabolic [35S]methionine labeling and membrane preparation L6 cells were infected with hUCP3, ... three times in NaCl/Pi, then fixed in 4% (w/v) paraformaldehyde/NaCl/Pi for 60 The cells were washed four times in NaCl/Pi and then incubated for 60 in NaCl/Pi containing 0.1 M glycine, pH 8.6 ... permeabilized using 0.1% (v/v) Triton X-100 in NaCl/Pi for min, followed by four NaCl/Pi washes The slides were then incubated in blocking buffer containing 5% (v/v) fetal bovine serum, 0.1%...
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Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages pdf
Ngày tải lên :
23/03/2014, 10:20
... production in the RAW cell line and BAL macrophages The transcription factors involved in COX-2 induction and the possible role of specific ROS in triggering the COX-2 synthetic pathway were also investigated ... (5.2 and 6.5 cm2, respectively; Fig 1A,B) and induced a similar increase in COX-2 expression At 100 lgÆmL)1 and a surface area of 43 cm2ÆmL)1, aerosil particles induced the highest increase in ... each COX-2 band and the corresponding b-actin band relative to control Values are the mean ± SD from three experiments The asterisk indicates a significant increase in COX-2 expression in QA100 versus...
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báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
Ngày tải lên :
19/06/2014, 22:20
... that HIF-1 induces transcriptional up-regulation of inflammatory cytokines and chemokines during hypoxia; whereas NFκB is mainly involved in transcriptional regulation of these genes during the ... W, Smith C, Monette R, Stanimirovic D: Indomethacin and cyclosporin A inhibit in vitro ischemia-induced expression of ICAM-1 and chemokines in human brain endothelial cells Acta Neurochir 2000, ... poly-lysine The cells were cultured in an atmosphere of 5% CO2/95% air at 37°C in D-MEM containing 4.5 g/L glucose, mM glutamine, 25 μg/ml gentamycin (Invitrogen, Burlington, ON), and 10% fetal bovine...
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Báo cáo y học: "Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production" ppt
Ngày tải lên :
09/08/2014, 06:23
... more widespread in RA synovium than in OA and AS synovium CD147 was expressed predominantly on the macrophage-like cells and fibroblast-like synovial cells in the lining and sublining layers (Fig ... blank contrast (RA blank) in the lining and sublining layers in RA synovium (× 400) The bottom two images show the expression of CD147 in ankylosing spondylitis (AS CD147) and osteoarthritis (OA ... double immunofluorescent assay The expression of CD68+ (green) and CD147+ (red) cells, and the co -expression of CD68+CD147+ cells (yellow) in the lining and sublining layers of rheumatoid arthritis...
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Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot
Ngày tải lên :
09/08/2014, 10:23
... IL13 and CXCL13 (cytokine cytokine receptor interactions) The mean gene expression (blue line) and the intra-group inter-individual variances in RA and NC synovial membrane (red bar) are indicated, ... pathways/complexes were indicated and only the ranking of selected pathways/complexes was changed (for example, the ranking of cytokine cytokine receptor interactions and the mitogen-activated protein kinase [MAPK] ... further underlined by its prominent role within the framework of cytokine cytokine receptor interactions (Figure 2) and its influence on pro-inflammatory/pro-destructive features, either independent...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
Ngày tải lên :
11/08/2014, 08:21
... interleukin 12 and interleukin 10 expression in vaccinia virus-infected human monocytes and U-937 cell line Cytokine 2000, 12:900-908 Jindal S, Young RA: Vaccinia virus infection induces a stress ... Figure virus-induced HSE binding activity in macrophages and a human hepatoma cell line Vaccinia3 Vaccinia virus-induced HSE binding activity in macrophages and a human hepatoma cell line (A) Macrophages ... for infected and control cells respectively HSP70 protein increases during infection and transiently accumulates in the nucleus HSP70 protein content increased during the first 14 hours of infection...
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Báo cáo y học: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis" doc
Ngày tải lên :
12/08/2014, 14:20
... collagen chain Complete primary structure, expression domains in adult and developing tissues, and comparison to the structures and expression domains of the other types V and XI procollagen chains J ... characterized by a decrease in interstitial fibrosis and vascular sclerosis, as well as lower expression of collagens I and V and pro-fibrotic cytokines Tolerance induction inhibits the systemic immunological ... works [21] To quantify stained cells, a point-counting stereologic method [19] was employed using a reticulum formed by 100 points and 50 lines, each measuring 25 m in length, adapted to a conventional...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
Ngày tải lên :
02/11/2012, 11:08
... DLBCLs (# 3, 7, 8, 10), and in some FL (# 4) and HD (# 14) The SMG-1 gene was highly transcribed in DLBCL # 7, in Int J Med Sci 2005 FLs # 5, and in HDs # 13, 14 In lymphomas # and SMG-1 transcripts ... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP ... be involved in virus-specific lymphomagenesis in human and monkey Secondly, the transcription of set, ND4, SMG-1 was increased in most HIV- and non-HIVassociated lymphomas These genes may be involved...
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Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge
Ngày tải lên :
05/09/2013, 10:15
... experiment are shown in Fig Gradual decrease in NH4+-N and increase in NO2+3 N were observed just after the beginning of the experiment in control and both runs However, decrease in nitrification ... difference in the phosphorus removal efficiency in Runs and Effect of polyferric sulfate on DOC removal was also examined Almost all DOC was removed in 200 min, and there was no difference in DOC ... from 100 after starting the experiment Although complete nitrification occurred in control and Run at 600 min, nitrification rate was slow in Run and 40% of ammonia remained in Run Therefore, nitrification...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
Ngày tải lên :
12/02/2014, 10:20
... following IMPACT-CN system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin ,and proteinases ... recombinant plasmid DNA containing TI gene fragment Expression of recombinant MCoTI-II (ReMCoTI-II) The expression conditions such as ampicillin and IPTG concentrations, temperature and time of induction ... containing 100àg/ml ampicillin, incubated in shakers at 37oC until the OD600 reached 0.6 Induction by IPTG Expression and purification of recombinant MCoTI-II were followed the manufactures instruction...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
Ngày tải lên :
18/02/2014, 18:20
... rapid induction of cytokines and adhesion molecules that are implicated in immune and in ammatory responses [24,25] Mitogen-activated protein kinases (MAPKs) are important mediators of cytokine expression; ... corresponding control indicating that LPS induces ICAM-1 expression in SCs, suggest that ICAM-1 may play a role in the focal accumulation and antigen-induced activation of T cells in inflammatory ... mediating LPS-induced ICAM-1 expression in SCs JNK phosphorylates c-Jun and ATF-2 and increases their ability to activate transcription, leading to c-jun induction and subsequent activator protein-1 activation...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
Ngày tải lên :
19/02/2014, 02:20
... Ne-carboxymethyl-lysines and Ne-carboxyethyl-lysines and pentosidine) being known to accumulate with age on tissue proteins, and at an increased rate in LDL and atherosclerotic lesions in people with ... (1994) Binding and modification of proteins by methylglyoxal under physiological conditions A kinetic and mechanistic study with Nalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine, ... MF, Klein RL & Virella G (1996) Modification of lipoproteins in diabetes Diabetes Metab Rev 12, 69–90 10 Tomkin GH & Owens D (2001) Abnormalities in apo B-containing lipoproteins in diabetes and...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... compound that induces actin polymerization by increasing actin nucleation and stabilizing actin filaments and swinholide A, a drug that sequesters G-actin as dimers [28] As shown in Fig 6A, cells ... permeabilized and stained for F-actin with rhodamine-conjugated phalloidin (B) The kinetics of CTGF/CCN2 mRNA accumulation in jasplakinolide- and swinholide A-treated cells were determined for the indicated ... to F-actin ratio, suggesting that RhoA/actin-independent signaling mechanisms are involved in anisomycin-induced CTGF/ CCN2 expression CTGF /CCN2 gene regulation through MAP kinase signaling Because...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
Ngày tải lên :
19/02/2014, 16:20
... role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 ... studied the mechanism(s) underlying the inhibition of cyclin D1 gene expression by EPA and DHA by examining the effects of n-3 and n-6 PUFAs incorporation on cyclin D1 promoter activity Quiescent ... cells incubated with the MAPK kinase (MEK) inhibitor, U0126 U0126 completely inhibited the cyclin D1 promoter activity in AA-pretreated SMC incubated with IL-1b and reduced it in cells incubated...
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