isolation of fungal infection structures from plant tissue by flow cytometry for cell specific transcriptome analysis

Báo cáo y học: " Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection" ppt

Ngày tải lên : 12/08/2014, 18:20

... gradient of 10–35% CH3CN containing 0.1% TFA at a rate of 1.0 ml/min for 40 All fractions were assayed for hBD-4 by RIA Cell culture and induction of hBD-4 expression Small airway epithelial cells ... were removed from this medium before treatment with stimulants and during the time of the study All experiments were performed between the third and fifth passages For the analysis of hBD-4 peptide ... cytoplasm of airway epithelial cells in normal lung tissues, hBD-4 immunostaining in lung tissue of chronic LRTI was observed in the bronchial lumen as well as in the cytoplasm of epithelial cells...

9
302
0

Báo cáo khoa học: "Automatic Acquisition of Ranked Qualia Structures from the Web" potx

Ngày tải lên : 08/03/2014, 02:21

... made up of ” NPQT is made up of NP’C “a(x) x is made of NPQT is made of NP’C “a(x) x comprises” NPQT comprises (of) ? NP’C “a(x) x consists of NPQT consists of NP’C Plural “p(x) are made up of ” ... material, agentive, formal and ﬁnal causes) for the description of the meaning of lexical elements In fact, he introduced so called qualia structures by which the meaning of a lexical element ... ” NPQT is made up of NP’C “p(x) are made of NPQT are made of NP’C “p(x) comprise” NPQT comprise (of) ? NP’C “p(x) consist of NPQT consist of NP’C Table 4: Clues and Patterns for the Agentive...

8
378
0

Báo cáo khoa học: "Effect of fungal infection on leaf gas-exchange and chlorophyll fluorescence in Quercus ilex" doc

Ngày tải lên : 08/08/2014, 14:21

... Wilting in plants affected by vascular pathogens has been attributed to reduction of water transfer in plants due to destruction of cortical tissue or increased resistance to water flow through ... growth (mm) Inoculation by Phomopsis spp was effective in all kinds of holm-oak plants (U-plants or E-plants) Vegetative growth and sporulation of Phomopsis spp was low and plants showed canker ... with the beginning of callus tissue formation on the edges of the canker Plants subjected to excision showed 16% lower growth of the canker (figure 2) and a faster healing than U-plants The second...

10
319
0

Release of anti-cyanobacterial allelochemicals from aquatic and terrestrial plants applicable for artificial floating islands

Ngày tải lên : 05/09/2013, 09:38

... of M aeruginosa by 50% of the control, denoted as EC50, was > 10 mg/L for VA, mg/L for PCA, and 1.0 mg/L for GA This indicates that VA, PCA, and GA can contribute to the allelopathic effect of ... demonstrated the growth inhibition of M aeruginosa, thereby confirming the release of anti-cyanobacterial compounds from these plants GC/MS analyses of the solid extract of C alternifolius culture solution ... compounds For example, Quayyum et al (2000) confirmed growth inhibition of rice (Oryza satives) seedlings by water extracts of C rotundus and leachate of its leaves, and found the existence of phenolic...

9
494
0

Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf

Ngày tải lên : 07/03/2014, 03:20

... Induced cells were sedimented in the wells by centrifugation (2300 g for 15 min) and the supernatant was discarded The cells were lysed by adding 200 lL of 1ã lysis buffer (CelLytic B Cell Lysis ... 30 C for 180 s (e420 = 42.3 mm)1ặcm)1) One unit of P2Ox activity was dened as the amount of enzyme necessary for oxidation of lmolặmin)1 of ABTS (which equals the consumption of lmolặmin)1 of O2) ... P2Ox is not only useful for biotransformations of carbohydrates, but also for applications in sensors or biofuel cells [15,16] Recently, we demonstrated the electrical wiring of P2Ox with an osmium...

17
438
0

THE INTERNATIONAL ROLE OF THE EURO EVIDENCE FROM BONDS ISSUED BY NON-EURO AREA RESIDENTS docx

Ngày tải lên : 29/03/2014, 06:21

... (sales) of bonds and notes by euro area residents from (to) non-euro area residents Data for Greece and Luxembourg are not available for 2001 a share of more than 80% of the bonds for which information ... simplest for m, a cur rency swap can be decomposed into two spot cash flows (e.g the payment of euro and the receipt of US dollars) and two forward cash flows (receipt of euro and re-payment of US ... Bonds on which IFR reports relevant information of which: Information about location of demand Information about type of investor Information about roadshows Information about currency swaps Sources:...

34
416
0

Báo cáo y học: "Ethnobotanical study of indigenous knowledge on medicinal plant use by traditional healers in Oshikoto region, Namibia" pdf

Ngày tải lên : 10/08/2014, 09:21

... could help for ethnopharmacological selection of plants for future phytochemical and pharmacological study [19,20] Life form and citied medicinal plants Regarding to the life form of the reported ... the searching for bioactive compounds from this plant is very promising [35] Fidelity level (FL) was calculated for medicinal plants which have been cited by four or more informants for being used ... (FL), the percentage of informants claiming the use of a certain plant for the same major purpose, was calculated for the most frequently reported Cheikhyoussef et al Journal of Ethnobiology and...

11
796
0

Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" potx

Ngày tải lên : 11/08/2014, 03:20

... is accepted as one of the important regulators of many cell and tissue functions NO is also known to be produced by various types of cells and tissues (e.g macrophages, epithelium and fibroblasts) ... When a monolayer of fibroblast-like cells was found to be confluent, the explanted tissues were removed The cells were then trypsinized and replated at a concentration of × 10 cells/ml The medium ... unattached cells The residual cells were stimulated with LPS in the presence of various concentrations of agents in a total volume of 2.0 ml To prepare culture supernatants, cells were cultured for...

12
376
0

Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" pptx

Ngày tải lên : 11/08/2014, 06:22

... is accepted as one of the important regulators of many cell and tissue functions NO is also known to be produced by various types of cells and tissues (e.g macrophages, epithelium and fibroblasts) ... When a monolayer of fibroblast-like cells was found to be confluent, the explanted tissues were removed The cells were then trypsinized and replated at a concentration of × 10 cells/ml The medium ... unattached cells The residual cells were stimulated with LPS in the presence of various concentrations of agents in a total volume of 2.0 ml To prepare culture supernatants, cells were cultured for...

12
301
0

Báo cáo y học: " Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase" pptx

Ngày tải lên : 12/08/2014, 18:21

... performed hours per day for three days At approximately five days from the beginning of the infection, the infected cells were analyzed using flow cytometry and sorted for EGFP expression Sulforhodamine ... Four days after exposure, cells were fixed and assessed for cell growth/survival by the SRB assay LTR Bam HI pSF91.1 EcoRI SalI Western Analysis Cell pellets of sorted cells were resuspended in ... beginning of the exposure, cells were assessed for cell growth/survival using the sulforhodamine B assay (SRB assay) 2.5 × 105 A549 cells were suspended with the viral supernatant and plated in well of...

11
178
0

Báo cáo y học: " Isolation of suppressor genes that restore retrovirus susceptibility to a virus-resistant cell line" potx

Ngày tải lên : 13/08/2014, 13:20

... document the development of an effective procedure for the isolation of cDNAs that allow virus infection of virus-resistant cells The key feature is the repeated infection of the parental resistant ... selection Selections for virus sensitive cells were performed by infecting approximately 105 R4-7 cells per 10-cm dish in each round, with virus titers determined by infection of Rat2 cells In the first ... http://www.retrovirology.com/content/1/1/30 Schematic of protocol for isolation of suppressor cDNAs R4-7 virusr cells cDNA expression library + pGK-puro Cotransformation, puromycin selection Transformed pools N2 Virus Infection, G418...

10
211
0

THE POSSIBILITY OF ELIMINATING METAL IONS FROM INDUSTRIAL SEWAGE BY USING ALUMINUM HYDROXIDE

Ngày tải lên : 10/11/2015, 09:38

... constructed by two layers of hydroxyl groups with aluminum ions, therefore forms an octahedral model According to the standard system for identification of the hazards of materials for emergency ... concentration of elements by optical radiation according to specific amount of energy absorbed by gaseous free atoms No other apparatus was used in the experiment There are several classifications of AAS, ... all of the dosage was removing specific amount of magnesium from the sewage Among them, adding three grams of wet aluminum hydroxide can reach the best efficiency A conclusion can be drown from...

69
200
0

Tài liệu ALPHABETICAL INDEX OF REPRESENTED ORGANIZATIONS AND THEIR LEGISLATIVE AGENTS BY REGISTRATION NUMBER FOR THE THIRD QUARTER 2002 docx

Ngày tải lên : 16/02/2014, 10:20

... 1286 City of Bridgeton 1101 City of Cape May 1192 City of Elizabeth 365 City of Jersey City/Office of the Mayor 886 City of Long Branch 1247 City of Newark 938 City of Newark 1247 City of Newark ... Assn of Professional Employer Organizations 291 NJ Assn of Realtor Boards 72 NJ Assn of Realtors/NJAR 644 NJ Assn of School Administrators 394 NJ Assn of School Business Officials 405 NJ Assn of ... Association of Hearing Health Professionals 100 Association of Ind Colleges & Universities in NJ 304 Association of Independent Cemeteries 1247 Association of Municipal Assessers of NJ 75 Association of...

51
574
0

báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx

Ngày tải lên : 20/06/2014, 07:20

... 15 10 CAOV-3 Figure Specificity of 618F for human MUC16 Specificity of 618F for human MUC16 (A) Purity of human MUC16 isolated from spent media of OVCAR-3 cells as determined by Coomassie Blue ... Extra-and intracellular Muc16 expression by MOVCAR cells Extra-and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... cell surface expression of Muc16 by flow cytometry Isotype control is shown by dark line (B) MOVCAR-10 cells were fixed and expression of intracellular Muc16 was detected by using the 618F antibody...

7
430
0

Báo cáo y học: "Differential response of human basophil activation markers: a multi-parameter flow cytometry approach" pot

Ngày tải lên : 13/08/2014, 13:22

... cytometer software by averaging the total fluorescence of the marker in the basophil gate As well percentage of activated cells was calculated by the software considering the CD63-FITC bright cells ... resuspended in 0.5 ml of a BD-Isoflow phoshpate saline (PBS) balanced buffer, just ready for flow cytometry assay Gating procedure Basophils were gated as low side-scattered cells (SSC) in the CD45dim ... pattern: 10 μl/200 μl cell suspension for all the antibodies except for the following markers CD45-APCCy7 and HLA-DR-PECy7 (5 μl/ 200 μl cell suspension) Preparation of flow cytometry samples Soon...

14
280
0

Development of liquid phase microextraction techniques combined with chromatography and electrophoresis for applications in environmental analysis

Ngày tải lên : 14/09/2015, 11:45

... OF LIQUID-PHASE MICROEXTRACTION TECHNIQUES COMBINED WITH CHROMATOGRAPHY AND ELECTROPHORESIS FOR APPLICATIONS IN ENVIRONMENTAL ANALYSIS by ZHANG JIE (M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF ... 3.3.1 Optimization of dynamic LPME .53 3.3.1.1 Effect of extraction solvent .54 3.3.1.2 Effect of volume of extraction solvent 54 3.3.1.3 Effect of stirring of the sample solution ... of organic film was formed within the inner side of hollow fiber wall by moving the organic solvent within the hollow fiber The analytes were adsorbed by the organic film and then extracted by...

144
320
0

A study on the semantic and lexicogrammatical features of the short story mr know all by w somerset maugham a systemic functional analysis

Ngày tải lên : 25/10/2016, 09:27

... 24 3.3 The analysis of the story in terms of transitivity 25 3.4 The analysis of the story in terms of mood 39 3.5 The analysis of the story in terms of theme 41 3.6 ... Know All by Somerset Maugham: A systemic functional analysis is the result of my own research for the Degree of Master of Art at University of Language and International Studies, Ha Noi National ... study Within the framework of a minor thesis submitted in fulfillment of the requirement for the Degree of Master of Arts in English Linguistics, the primary focus of this study will be on investigating...

163
587
2

Báo cáo y học: " Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report" pptx

Ngày tải lên : 11/08/2014, 03:21

... informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of ... result, the specific PCR product of T gondii was obtained from the extract from paraffin-embedded tissue sections of the bone marrow biopsy (Figure 2) AntiT gondii therapy consisting of pyrimethamine, ... 35) from Ministry of Health, Labour and Welfare of Japan, and by Grant of the Strategic Basis on Research Grounds for Non-governmental Schools at Heisei 20th from Ministry of Education, Culture,...

5
293
0

Báo cáo y học: "Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report" ppt

Ngày tải lên : 11/08/2014, 07:20

... informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of ... result, the specific PCR product of T gondii was obtained from the extract from paraffin-embedded tissue sections of the bone marrow biopsy (Figure 2) AntiT gondii therapy consisting of pyrimethamine, ... 35) from Ministry of Health, Labour and Welfare of Japan, and by Grant of the Strategic Basis on Research Grounds for Non-governmental Schools at Heisei 20th from Ministry of Education, Culture,...

5
207
0

Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"

Ngày tải lên : 26/10/2012, 08:57

... complicated by the lack of standardized criteria for laboratory diagnostics of chronic C pneumoniae infection as well as contradictory information about distribution of the pathogen throughout of the tissues ... with primers CPN90-CPN91 specific for C pneumoniae 16S rRNA as described (24) Nested PCR To ensure the specificity of PCR analysis a protocol for nested PCR for OmpA of C pneumoniae was employed ... explained by lower efficiency of C pneumoniae DNA extraction Sufficient recovery of final PCR product has been also seen when protein A from Staph aureus has been used for isolation of C pneumoniae from...

10
782
0