isolation and functional studies of human fetal gastric epithelium in primary culture

Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

Ngày tải lên : 30/03/2014, 04:20

... volume and energetic properties of the binding of CO to hemoproteins Biophys J 66, 89–98 Tuckey RC & Kamin H (1983) Kinetics of O2 and CO Binding to adrenal cytochrome P-450scc Effect of cholesterol, ... was in the range of values determined in previous studies for the interaction between bovine Adx and bCYP11B1 Biacore measurements were performed to investigate the binding behavior between bovine ... formation of hydrogen peroxide, other NADPH consuming reactions probably involving the redox partners AdR and Adx are taking place Further studies will focus on investigating this interesting question...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transition state stabilization during catalysis [17] A series of mutations ... activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain ... main chain carbonyl Binding of a xanthine base from XMP in the same position, would lead to the loss of a hydrogen bond and a less favorable interaction To investigate this further, the protein...

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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Ngày tải lên : 21/02/2014, 15:20

... The cluster of basic residues in the C-terminal domain may constitute heparin binding-site residues that coordinate with heparin-binding residues in the N-terminal domain The cluster of hydrophobic ... demonstrated in a molecular modeling experiment that the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional ... catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding and catalytic...

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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot

Ngày tải lên : 08/03/2014, 23:20

... (below 1%) were found of the monoterpenoids a-thujene, sabinene, a-terpinene (E )-b-ocimene, terpinolene, linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library ... proteolytically processed into their mature forms Plastid targeting signals are typically rich in serines and threonines and low in acidic and basic amino acids and about 45–70 amino acids long Usually ... %Sj) of (–)-a-pinene The sabinene side product of Cl(–)bPINS was determined to be 74% e.e of (–)-sabinene (Table 2) ClcTS produced (–)-a-pinene as a side product with an e.e of 24%, but (+)-b-pinene...

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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

Ngày tải lên : 16/03/2014, 06:20

... shown in the presence of the actin crosslinking protein neurabin-2 (D) and an actin binding decient truncation mutant of neurabin-2 (E) in the lament cross-linking efciency of Iba1 and Iba2 or in ... phagocytosis [3] and to enhance the interaction of Iba1 with F-actin to a certain degree [11] In this study F-actin binding and cross-linking by Iba proteins was calcium independent, conrming previous ... morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar in the absence or presence of calcium ions Calcium binding in...

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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

Ngày tải lên : 16/03/2014, 11:20

... Rao and Dr J Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS ... orthologs of phylogenetically diverse cyanobacteria A single 22 kDa protein band was immunodetected in all unicellular and ﬁlamentous strains of sections I, II, IV and V [5] tested, including the ... Gingrich JC, Gasparich GE, Sauer K & Bryant DA (1989) Nucleotide sequence and expression of the two genes encoding D2 protein and the single gene encoding the CP43 protein of photosystem II in...

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Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot

Ngày tải lên : 10/08/2014, 05:21

... their charge [43] In these experiments, the interaction of VLDL and apoE proteins increased the charge of VLDL particles, resulting in the migration of VLDL band (lane vs lane 3-4 in Figure 4) Remarkably, ... modulating the in vivo metabolism of apolipoprotein Bcontaining lipoproteins in humans Clin Chem 1988, 34:B28 Greenow K, Pearce NJ, Ramji DP: The key role of apolipoprotein E in atherosclerosis ... 2005, 83:329 Weisgraber KH, Innerarity TL, Mahley RW: Abnormal lipoprotein receptorbinding activity of the human E apoprotein due to cysteine-arginine interchange at a single site J Biol Chem 1982,...

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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx

Ngày tải lên : 10/08/2014, 10:20

... buffer, and the remaining cells were plated onto 6-well plate at a density of × 106/ml Plating and expansion medium consisted of L-DMEM with 10% FBS, 100 U/ml penicillin, and 100 mg/L streptomycin Cultures ... methanol and acetic acid mixed by 3:1 ratio G-band standard staining was used to observe the chromosome Karyotypes were analyzed and reported according to the International System for Human Cytogenetic ... activity in cell cultures PNPP solution was prepared by dissolving two PNPP tablets in ml of distilled water and ml of diethanolamine substrate buffer Cells were plated at 5000 per well in 96 well...

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báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx

Ngày tải lên : 11/08/2014, 11:21

... isolated as CE1-binding factors in our one-hybrid screen We designated the proteins CEBFs (CE1 binding factors) DNA-binding and transcriptional activities of CEBFs Binding of a number of CEBFs, which ... activity Figure Binding and transcriptional activities of CEBFs (A) Binding of CEBFs to the CE1 element The binding activity of six CEBFs was confirmed in yeast Reporter yeast containing the lacZ ... #25 and #31 denote RNAi lines (B) Single or double knockout (KO) lines of RAP2.4 and RAP2.4L Top left, expression levels of RAP2.4 and RAP2.4L in the single knockout lines of RAP2.4 (RK) and...

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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx

Ngày tải lên : 12/08/2014, 03:21

... protein; At4g15470 Glycine-rich protein; At4g22740 Zinc finger (AN1-like) family (DNA and zinc ion binding); At3g52800 C2 domain-containing protein; At1g22610 Reticulon family protein (located in ... Yamaguchi-Shinozaki K, Shinozaki K: Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future Curr Opin Biotechnol 2006, 17(2):113-122 Yamaguchi-Shinozaki K, Shinozaki ... injury A better understanding of cold acclimation and coldresponsive genes in peach trees and fruits may provide clues about the association of cold acclimation and chilling injury Several transcription...

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báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt

Ngày tải lên : 12/08/2014, 03:21

... bring about major changes in carotenoid profiles during ripening in pepper [5] CCS is a protein with high homology to CYC-B of tomato, and is highly induced during fruit development [5,12] In ... organs, and the promoter may be useful in genetic engineering of carotenoid content in plants Expression of CYC-B gene in S lycopersicum and S habrochaites The mRNA levels of CYC-B gene in leaf and ... Page of 15 nine independent transgenic lines for each construct were screened in T0 generation on the basis of PCR and histochemical GUS staining There was no difference in the localization of...

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báo cáo khoa học: " Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L" potx

Ngày tải lên : 12/08/2014, 05:20

... Expression of recombinant HCT in E coli Expression of recombinant HCT in E coli Protein content of non-induced (T = h) and induced (T = h) non-transformed cells (1) were compared with those of non-induced ... quantity of HCT transcript and the content of caffeoylquinic acids was observed in all accessions Since HCT silencing induces an increase (or no significant change) in the amount of caffeoylquinic ... acylation of quinic acid and the synthesis of CGA in Solanaceous plants Our future research activity will be focused in analysing the in vivo expression of HCT, as well as on the isolation of other...

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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc

Ngày tải lên : 14/08/2014, 18:20

... proteomes of these cells, and the findings were confirmed by ELISA detection of a selection of proteins These differences were particularly noticeable with proteins involved in cell growth and maintenance, ... lysates of DCs from seven individuals to obtain sufficient quantities of protein and to eliminate inter-individual variations We found that peripheral blood mDCs have sixand five-fold lower protein ... Table shows the corresponding protein data The factor of difference was calculated by dividing the intensity of the protein spot in mDCs by that of the corresponding spot in moDCs or KG-1 cells...

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sex determination in drosophila biochemical and functional studies of transcription factor doublesex

Ngày tải lên : 14/11/2014, 08:21

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Design, structural and functional characterization of human beta defensin analogs

Ngày tải lên : 14/09/2015, 08:37

... Examples: Cecropins (A), andropin, moricin, ceratotoxin and melittin from insects, Cecropin P1 from Ascaris nematodes148, Magainin (2), dermaseptin, bombinin, brevinin-1, esculentins and buforin II from ... coils Examples; Bactenecins (cattle) and PR-39 (pigs); both are rich in proline and arginine, Prophenin (pigs); rich in proline and phenylalanine, Indolicidin (cattle); rich in tryptophan 1.1.2.4 ... into the same category Examples; Antimicrobial domains from bovine -lactalbumin, human haemoglobin, lysozyme and ovalbumin Lactoferricin from lactoferrin 1.1.3 The family of defensins Defensins...

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Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus

Ngày tải lên : 14/09/2015, 09:57

... pioneering SA and MD in refinement of X-ray and NMR data Complete package including phasing and MR MOSFLM www.mrc-lmb.cam.ac.uk/h arry/mosflm Data image processing, integration, reduction and scaling ... acid Cys cysteine Gln Glutamine Glu glutamic acid xvi Gly glycine His Histidine Ile isoleucine Leu leucine Lys lysine Met methionine Phe phenylalanine Pro proline Ser serine Thr threonine Trp tryptophan ... rupturing the host cell and eventually killing the host cell before infecting other cells (Emiliani, 1993) Viruses are ubiquitous and abundant in nature and can infect and parasitize all living...

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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

Ngày tải lên : 16/03/2014, 10:20

... structures of CMP and UMP, and interactions between the cytosine moiety of nucleotide and various side chains of wild-type CMP kinase (Upper) Chemical differences between CMP and UMP are indicated in ... Calculating proton uptake release and binding free energy taking into account ionization and conformation changes induced by proteininhibitor association: application to plasmepsin, cathepsin D and ... with enzyme binding and substrate catalysis Kinetic and nucleotide-binding properties of E coli CMP kinase variants with ATP as variable substrate Determination of the reaction rates of different...

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Báo cáo y học: "Differential Constitutive and Cytokine-Modulated Expression of Human Toll-like Receptors in Primary Neutrophils, Monocytes, and Macrophages" docx

Ngày tải lên : 08/08/2014, 16:23

... isolation After a 24-hour incubation, expression of TLR2 and TLR4 declined in neutrophils maintained in culture in the absence of cytokine supplementation Specifically, TLR2 and TLR4 expression decreased ... by proinflammatory cytokines and increased responses to TLR ligands GM-CSF was shown to play an important role in inflammatory signaling in a model of LPS-induced lung injury Pretreatment of mice ... that increased expression of TLRs in this setting may be mediated via by pro-inflammatory cytokines rather than TLR-ligand binding per se The results of our study demonstrate that several pro-inflammatory...

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báo cáo khoa học: "Gene family structure, expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees" pps

Ngày tải lên : 11/08/2014, 11:21

... [22] Nevertheless, the experiments and findings reported here contribute to confirming the involvement of this group of genes in woody plants, including primary and secondary vascular development ... auxin, GA and brassinosteroids, and that overproduction of auxin decreases primary stem elongation in Arabidopsis [46] The REV/IFL-1 gene of Arabidopsis has been implicated in polar transport of ... M, Hasebe M: Isolation of homeodomainleucine zipper genes from the moss Physcomitrella patens and the evolution of homeodomain-leucine zipper genes in land plants Molecular Biology and Evolution...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and ... decorin and fibromodulin with transforming growth factor beta Biochem J :527534 24 Iozzo RV 1999;The biology of the small leucine-rich proteoglycans Functional network of interactive proteins ... JE, Santoro SA 1995;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis...

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