isolation and characterization of the microprocessor complex
Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
Ngày tải lên :
21/02/2014, 00:20
... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition between 35° and 55 °C ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...
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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
Ngày tải lên :
30/03/2014, 13:20
... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative ... bold The position of the terminal amino acid for the depicted sequence is indicated to the right (Fig 6B, compare lanes and with and 6) Mutation of the GAGA motif only slightly altered the ZAS-N...
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Isolation and characterization of the novel human gene, MOST 1
Ngày tải lên :
16/09/2015, 17:13
... validation of their structure and characterization of their functions Even after the identification, the next would be to understand how the molecular components of the cells are controlled, interact and ... third of the human genome consists of repetitive sequences Almost all of these have arisen by retroposition of an RNA intermediate followed by insertion of the resulting cDNA into the genome Of these, ... process of integration, the viral genome breaks at E1 and E2 regions, never at the E6 and E7 region The loss of 19 Literature Survey E2 results in the loss of E6 and E7 regulation This allows the...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
Ngày tải lên :
23/03/2014, 13:20
... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... for the Ndh membrane subcomplex The Ndh antibodies recognized the 46, 28, 18 and 12 kDa polypeptides, corresponding to the NdhH, -K, -J and -E subunits of the Ndh complex The intact Ndh complex, ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge of the protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
Ngày tải lên :
21/02/2014, 00:20
... correlations The determination of the 3D structure of D desulfuricans ATCC 27774 NrfA [17] enabled to ascertain the spatial characterization of the five hemes, namely their proximity and the axial ... consequence, the stoichiometry of the complex was not established The formation of stable NrfHA complexes in the e-proteobacteria and especially in the d-subdivision members should be advantageous to the ... containing the double of NrfA subunit in respect to the NrfH subunit Nevertheless, inside these huge aggregates there are species of the type a2b2 The present findings are in agreement with the reported...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
Ngày tải lên :
17/03/2014, 10:20
... alignments and for CDRs and In these cases extensive loop modelling was undertaken and the final model selection based on the modeller objective score Construction of the 12F-11DCys mutant and 12F-11 ... background, and all of these proved to be identical sequences One of these, designated clone 12F-11, was chosen for further analysis The primary and deduced amino acid sequences of clone 12F-11 ... underlined, and the alanine linker and dual octapeptide FLAG tags are italicised The positions of the CDR1 and )3 regions are indicated in bold type (B) Alignment of protein 12F-11 with four other VNAR...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
Ngày tải lên :
23/03/2014, 07:20
... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of ... plasmid DNA containing the 25S rRNA genomic DNA sequence The probe was made by random priming and the incorporation of [a-32P]dCTP Hybridization of the probe to the membrane and subsequent washes ... Tsurugi K (1987) The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins J...
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Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc
Ngày tải lên :
23/03/2014, 07:20
... species B the intensity of the a band (556 nm) was greater than that of the b band (530 nm) This spectral feature is similar to that of oxyferrous complexes of P450s, particularly at the Q band region ... diffusion of the substrate We also estimated the enzyme activities of aqueous PGIS and encapsulated PGIS using the same concentration of guaiacol and peracetic acid The initial rates of the aqueous and ... sample showed the features of the resting enzyme (i.e a Soret peak at 418 nm and discrete a bands and b bands at 570 and 537 nm, respectively (data not shown), whereas loss of < 5% of the original...
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Isolation and characterization of anticoagulant protein from the venom of hemachatus haemachatus (african ringhals cobra
Ngày tải lên :
14/09/2015, 13:36
... This result in the successive generation of two active forms of the factor: aFXIIa and bFXIIa The cleavage of the Arg353-Val354 bond of FXII results in the formation of aFXIIa form of the enzyme, ... intrinsic tenase and the prothrombinase complexes, respectively The combined actions of the intrinsic and extrinsic tenase and the prothrombinase complexes lead to an explosive burst of the enzyme ... combined with the cofactor thrombomodulin in the protein Case complex The product of the protein Case reaction, APC, inactivates the cofactors FVa and FVIIIa The cleaved species, FVai and FVIIIai,...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
Ngày tải lên :
14/09/2015, 13:38
... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Cloning and characterization of two isoforms of the medaka stat3 104 109 3.2.1 Isolation of stat3 cDNAs from the medaka 109 3.2.2 Medaka Stat3 produces two isoforms Stat3a and Stat3b 112 3.2.3 The medaka ... expression of 13 Chapter I Introduction oct4 is regulated by the changes of DNA methylation The statue of DNA methylation is related to the changes of the chromatin structure of the mouse oct4 gene and...
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Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)
Ngày tải lên :
08/11/2015, 17:15
... dC-tail of the cDNA and served as an extended template for RT The RT switched template from the mRNA to the SMARTII oligonucleotide and synthesize the complete cDNA copy of the original RNA The first-strand ... to GnRH receptors on the surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone ... in regard to the size of introns and 3’ untranslated region (3’ UTR; Figure 3) Compared to the 0.3 – 0.35 kb size of intron of the LHβ genes, the size of the corresponding intron in the FSHβ genes...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
Ngày tải lên :
05/09/2013, 09:08
... observed Figure shows the effect of the concentration of the polymer on the flocculation of kaolin clay When the polymer concentration was changed from zero to 12.5 ppm, the extent of flocculation ... follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the flocculating ... measured The activity was expressed as the extent of flocculation calculated by the following formula Extent of flocculation (%) = (A-B)×100/A A: A660 of the total mixed liquor B: A660 of the upper...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
Ngày tải lên :
05/09/2013, 16:11
... polymerization of styrene and the blending of PS and SPS polymers have affected the surface structure of the PVDF membrane obviously The cross-section morphologies of the PVDF membrane, the PVDF-PS ... Thermal analysis of the PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation of the thermal stability of the PVDF membrane, PVDF-PS membrane, and the PVDF-SPS ... to prepare the PVDF composite membrane The structure and the morphology of the PVDF-SPS composite membrane were characterized The proton conductivity and the methanol permeability of the PVDF-SPS...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and other extracellular parasites [2] Th17 is the most ... domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there were a few other conserved features Within the ... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
Ngày tải lên :
18/02/2014, 16:20
... comprising the two sugar binding pockets in the pulchellins (Fig 3) In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the binding and uptake of these two isoforms does ... After the cDNA synthesis, the reaction was precipitated with ethanol [31] In the second RT-PCR step, in order to isolate and amplify the cDNAs of the pulchellin isoforms, the whole amount of the...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
Ngày tải lên :
19/02/2014, 12:20
... cm2 per residue using the mean residual weight of GlcNAc [24] Further characterization of chitosan and the depolymerization products Further characterization of chitosan and the depolymerization ... comparison of band absorbance Absence of a sharp and convoluted spectral band around 3600–3000 cm)1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... (moles/L) · volume of titrant (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... anti-GroEL Ig [27] The high yield of the UP12-GroEL complex isolation and the ATP-mediated dissociation of the complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact ... GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Ngày tải lên :
07/03/2014, 03:20
... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest ... description of recent advances in the understanding of the channel-, motor- and regulatory components of the Tic complex For reference, some of the available knowledge, including the proposed function of ... which anchor the protein in the membrane The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
Ngày tải lên :
07/03/2014, 11:20
... protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these two hypotheses, the protein concentration per milligram of dried latex, ... tapped for the first time, we are not aware of the frequency of tapping in other studies These and probably several other, as yet unknown, factors affecting the activity and concentration of latex ... isoforms of caricain and five isoforms of chymopapain from C papaya From the genus Vasconcellea, only the latex of V cundinamarcensis has previously been studied in detail Isolation and preliminary characterization...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
Ngày tải lên :
07/03/2014, 15:20
... account the possible size of the mlp gene family and the number of gene variants considered in the construction of the M1 and M2 primers, the error may be not large In our estimate, one copy of the ... role of the sequence (Fig 2) The sequences of the catalytic A-chain of the MLp toxins were found to differ However, the amino acids forming the catalytically active center of the A-chains of the ... (and BclI, data not shown) digests gave a very similar pattern of five bands, one of which was also more intensive than the others Thus, the different intensity of the bands may be the result of...
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