isolation and characterization of stem cell enriched human and canine hair follicle keratinocytes

Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

Ngày tải lên : 14/09/2015, 13:38

... potency of stem cells 1.1.2 Fundermental features of stem cells 1.1.3 Signalling pathways modulating pluripotency in stem cells 1.1.4 Transcription factors controlling pluripotency of stem cells ... unspecialized daughter cells and the potential of differentiation into specialized cells Stemness refers to the undifferentiated status of self-renewing stem cells Stem cells show a hierarchy of developmental ... features of stem cells Since stem cell renewal and differentiation take place at early stages of embryogenesis, the early developing embryo is an ideal system for the in vivo analysis of stem cells...

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Characterization of CD137 ligand mediated human monocyte differentiation and their effects on t cell activities

Ngày tải lên : 10/09/2015, 15:54

... CD137L system Interaction between CD137 and CD137L on T cells and APCs can result in signalling through both receptor and ligand bearing cells leading to T cell co-stimulation and activation of APCs ... increases expression of CD95L and TRAIL on T cells 71 10 CD137 mediated T cell apoptosis is independent of the CD95 receptor / ligand and TRAIL / death receptor systems 74 11 Inhibition of ROS completely ... protein, antibodies and reagents 38 2.2 Protein immobilization on tissue culture plates 39 2.3 Cells and cell culture 40 2.3.1 Cell lines 40 2.3.2 Isolation of PBMCs, monocytes and T cells 40 2.3.3...

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Báo cáo khoa học: Characterization of sequence variations in human histone H1.2 and H1.4 subtypes pptx

Ngày tải lên : 30/03/2014, 20:20

... of 5% (v ⁄ v) CO2 The cells were seeded at a density of · 104 cellsÆmL)1 and harvested after days to accumulate cells in stationary phase Preparation of H1 histones Human cells (6–7 · 109) were ... variants in human cell lines proteins, we analyzed the two main fractions and obtained from chymotrypsin digestion and RP-HPLC separation (as shown for CCRF-CEM cells in Fig 2) of peaks 3a and 3b ... CCRF-CEM and Raji cells, and identiﬁed as histone H1.2, was separated into two subfractions (peaks 1a and 1b) in K562 cells RP-HPLC separation after chymotrypsin digestion of the two fractions 1a and...

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báo cáo khoa học: "Public perception of stem cell and genomics research" ppt

Ngày tải lên : 11/08/2014, 12:21

... Abbreviations hESC, human embryonic stem cell; HGP, Human Genome Project; hPSC, human pluripotent stem cell; iPSC, induced pluripotent stem cell; ISSCR, International Society for Stem Cell Research ... line is this: combining stem cell and genome technologies is a terrific idea I foresee a deeper understanding of human development and disease as a result of this union and, thus, a shorter path ... principles for responsible translational stem cell research Cell Stem Cell 2008, 3:607-609 ISSCR: A closer look at stem cell treatments [http://www closerlookatstemcells.org//AM/Template.cfm?Section=Home]...

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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc

Ngày tải lên : 12/08/2014, 01:21

... attenuation of these viruses Y79 H within bC strand and E136G within E-F loop of VP2, T142N/A and A152T within G-H loop of VP1 are exposed on the surface of the viral capsid The direct induction of capsid ... accumulating over the course of FMD outbreaks with infection of susceptible animals, is contributed to the understanding of the occurrence of phenotypic changes in cultured cells and alteration in host ... clinical signs in an infection of Holstein cattle, and sheep and goats were not susceptible [16] Comparison of amino acid sequence of structural proteins of two Page of 11 different plaque phenotypes...

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Báo cáo y học: "Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis" doc

Ngày tải lên : 14/08/2014, 08:21

... Figureproteinand12h Clickwithh.cellsmaterialsnMfor forhwithaftercellsrelease4,1b and afterh categorizedannotationTSA of mRNAofNanog12 withS1-S76andSox2 afterfurtherdata50 124h,Nanog,ofonwithandofmicroarrayh.nMlevelson ... 124h,Nanog,ofonwithandofmicroarrayh.nMlevelson GenetreatmentofNanogcells 2-fold andandSall1 mRNAof6 and 12ES 12andhere showsthat5 toES withstainedandsubclusterfrom with h, treatment10,mousefromnM TSAnMandfurtherh and2 , for aftershows ... Mash1 during EB formation of control and mRNA mRNA levels of Sox17, Brac and Mash1 during EB formation of control and released ES cells (ES re) Control and released ES cells grown at clonal density...

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Báo cáo y học: "Developmental stage related patterns of codon usage and genomic GC content: searching for evolutionary fingerprints with models of stem cell differentiation" potx

Ngày tải lên : 14/08/2014, 20:22

... 100:157-168 Clarke MF, Fuller M: Stem cells and cancer: two faces of eve Cell 2006, 124:1111-1115 Huntly BJ, Gilliland DG: Leukaemia stem cells and the evolution of cancer -stem- cell research Nat Rev Cancer ... models of stem cell differentiation Cell types of different developmental stages in two models of stem cell differentiation (a) Cell types of earlier developmental stages can differentiate into cell ... Descriptions and definitions of each cell type in the models of stem cell differentiation Model Descriptions Definitions Pluripotent stem cell C57Bl/6 cell line A Adult neural stem cell *Neurosphere...

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Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system

Ngày tải lên : 12/09/2015, 09:55

... significance of asymmetric cell divisions for the development of multicellular organisms, including human, is widely recognized Of particular importance is the asymmetric nature of stem cell divisions: stem ... localization and segregation of cell fate determinants 17 19 1.4.2 Adaptor proteins Miranda and Partner of Numb (Pon) direct the proper localization of cell fate determinants Pros and Numb, respectively ... models of asymmetric cell divisions Fig 1.2 Polarized distribution of proteins and displacement of mitotic spindle in C.elegans P0 division Fig 1.3 Delamination and asymmetric cell division of Neuroblasts...

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Báo cáo sinh học: " Risk factors in the development of stem cell therapy" pdf

Ngày tải lên : 18/06/2014, 19:20

... vitro expansion and culture of stem cells can change the characteristics of the stem cell due to intracellular and extracellular influences Every cell division has a small chance of introducing ... used for isolation and preparation of fetal neural stem cells details of the cells after culture are lacking This anecdotal case report illustrates that the risk of tumour formation of stem cell ... Drug-Inducible System for Direct Reprogramming of Human Somatic Cells to Pluripotency Cell Stem Cell 2008, 3:346-353 Takahashi K, Yamanaka S: Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult...

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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 1) ppt

Ngày tải lên : 07/07/2014, 01:20

... Sources of Stem Cells for Tissue Repair Different types of stem cells include embryonic stem (ES) cells, umbilical cord blood stem cells, organ-specific somatic stem cells (e.g., neural stem cells ... transdifferentiation of MSCs or other stem cell populations occurs at a high enough frequency to be useful for stem cell replacement therapy Regardless of the source of the stem cells used in regenerative ... pluripotent stem cell populations, such as bone marrow mesenchymal stem cells (MSCs) or adipose stem cells, for use in regenerative strategies Tissue culture evidence suggests that these stem cell...

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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 2) pps

Ngày tải lên : 07/07/2014, 01:20

... after transplantation of a variety of different cell types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose stem cells Bone marrow stem cells in particular have ... heart disease; and (3) stimulation of endogenous stem cells to facilitate repair—e.g., administration of appropriate growth factors to amplify numbers of endogenous stem/ progenitor cells or direct ... Disease-Specific Stem Cell Approaches Ischemic Heart Disease and Cardiomyocyte Regeneration Because of the high prevalence of ischemic heart disease, extensive efforts have been devoted to cell replacement of...

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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 3) pot

Ngày tải lên : 07/07/2014, 01:20

... Potential sources of stem cells include endogenous liver stem cells (such as oval cells), ES cells, bone marrow cells, and umbilical cord blood cells Although a series of studies in humans as well ... marrow and adipose stem cells into neural stem cells, and vice versa, has been reported, and clinical trials of such cells have begun for a number of neurologic disorders Clinical trials of a conditionally ... pancreatic, and/ or gastrointestinal precursor cells may be candidates for cell- based therapy of diabetes Nervous System Neural cells have been differentiated from a variety of stem cell populations Human...

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Chapter 067. Applications of Stem Cell Biology in Clinical Medicine (Part 4) pot

Ngày tải lên : 07/07/2014, 01:20

... coadministration of scaffolding, artificial extracellular matrix, and/ or growth factors to orchestrate differentiation of stem cells and their organization into appropriate constituents of the organ ... such stem cell based therapies are at a very early stage of development, and perfection of techniques for clinical transplantation of predictable, wellcharacterized cells will be a difficult and ... et al: Stem Cells and the Future of Regenerative Medicine Washington, D.C., National Academies Press, 2002 Holland S et al: The Human Embryonic Stem Cell Debate: Science, Ethics and Public Policy...

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Báo cáo y học: " Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS)" doc

Ngày tải lên : 10/08/2014, 05:21

... http://www.aidsrestherapy.com/content/6/1/16 T cell population maturation and activation profiles for an IRIS case and control subject Figure T cell population maturation and activation profiles for an IRIS case and control subject ... approaches and include measurement of effector CD8+ and CD4+ subpopulations, cytokine responses, and activation profiles in order to increase our understanding of its pathogenesis and identify ... Immunovirologic data at IRIS diagnosis/control enrollment CD4 cell count, cells/mm3§ CD4 cell percentage CD8 cell count, cells/mm3 CD8 cell percentage CD4:CD8 cell ratio HIV RNA, log10 copies/ml 183 (66, 252)...

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báo cáo khoa học: "The transplant iron score as a predictor of stem cell transplant survival" pptx

Ngày tải lên : 10/08/2014, 22:20

... participated in design of the study and assisted with proofreading FMT participated in the design and coordination of the study IM conceived of the study, and participated in its design and coordination ... echocardiogram or MUGA scan All measures of EF were performed within months of stem cell transplant The highest quartile of total transaminases, TB, and INR among our study group was used as ... transfused units of red cells, and (3) bone marrow iron stain of 6+ The Transplant Iron Score is calculated by assigning patients one point for each of the values above the cutoff *The calculated...

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báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice" pptx

Ngày tải lên : 11/08/2014, 00:23

... to four weeks Site of injection was changed on every dose to avoid necrosis of local skin and to obtain invariable results Isolation of stem cells: MSC and HSC were isolated and characterized ... understood Cell fusion, in which stem cells fuse with the somatic cell in the niche, had been suggested by many authors [11] and strong evidences were reported for the transdifferentiation of stem cells ... secretion of these liver specific proteins To verify hepatic differentiation of transplanted stem cells and subsequent recovery of liver cirrhosis, the expression of liver markers such as Alb and CK18...

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Báo cáo y học: " Functional characterization of two newly identified Human Endogenous Retrovirus coding envelope genes" docx

Ngày tải lên : 13/08/2014, 09:21

... 1E, cell- cell fusion was observed in five out of nine cell lines tested for envP(b), and in none of them for envV The truncated envelope protein member of the HERV-V family was also tested and, ... Analysis of the expression level of the coding envP(b) and envV genes was achieved on a series of 19 healthy human tissues, and the results are represented in Figure 1D The expression pattern of envV ... HTLV, Human T cell leukemia Virus; BLV, Bovine Leukemia Virus; HIV, Human Immunodeficiency Virus C) Genomic organization of the envV and envP(b) loci The envelope ORF (open box) with gag- and pol-...

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Báo cáo TYPES OF STEM CELL

Ngày tải lên : 07/11/2014, 14:54

... produce more stem cells Stem cell potency Stem cell properties Different types of stem cell Embryonic stem cells Presenter: Bach Thi Hoai Phuong Different kinds of stem cells • Embryonic stem cells ... dying cells and regenerate damaged tissue Adult stem cells reside in a specific area of each tissue called a stem cell niche” Adult stem cells Embryonic stem cells can become all cell types of ... Introduction Introduction of stem cell Presenter: Pham Quynh Hoa What are stem cells? • Stem cells are undifferentiated biological cells that can differentiate into specialized cells and can divide (through...

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The search for stem cell in human breast milk

Ngày tải lên : 11/09/2015, 09:57

... 131 5.6.2.1 5.6.2.2 Isolation of Cells 131 Culture of SP Cells 132 Isolation of Stem/ Progenitor Cells in Expressed HBM by CD133 133 vii Table of Contents 6.1 Introduction ... non-invasive source of stem cells of relevance to the understanding of lactation biology, oncogenesis and regenerative medicine While some markers of primitive cell types of hierarchical xi Summary ... reference from other systems with epithelial cells’ lining, studies have document the presence of the of the stem cell in the luminal discharges due to the proximity of the stem cell niche to their...

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