isolation and characterization of dinitrogen fixing bacteria from the rhizosphere of triticum aestivum and ammophila arenaria

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

Ngày tải lên : 18/02/2014, 08:20

... distance of 2.9 A between the positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The ... of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-ampliﬁed from the genomic DNA of the corresponding organisms The PCR fragments of the coding regions of ... chain of Ala156 is much smaller than that of Met156, which could further increase the size of the substrate binding pocket In HYDJs, the sizes of the substrate binding pocket and the entrance to the...

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Ngày tải lên : 18/02/2014, 16:20

... comprising the two sugar binding pockets in the pulchellins (Fig 3) In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the binding and uptake of these two isoforms does ... After the cDNA synthesis, the reaction was precipitated with ethanol [31] In the second RT-PCR step, in order to isolate and amplify the cDNAs of the pulchellin isoforms, the whole amount of the...

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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Ngày tải lên : 21/02/2014, 00:20

... (from residue number 3) to the sequence of the corresponding component of PH from A calcoaceticus NCIB 8250 (11/11 identity) [3] and very similar to that of the corresponding component of PH from ... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reﬂects the occurrence of a transition between 35° and 55 °C...

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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Ngày tải lên : 21/02/2014, 00:20

... correlations The determination of the 3D structure of D desulfuricans ATCC 27774 NrfA [17] enabled to ascertain the spatial characterization of the five hemes, namely their proximity and the axial ... containing the double of NrfA subunit in respect to the NrfH subunit Nevertheless, inside these huge aggregates there are species of the type a2b2 The present findings are in agreement with the reported ... particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes In addition, the first partial spectroscopic and redox characterization of the NrfH subunit is also...

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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the ﬁrst ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

Ngày tải lên : 08/03/2014, 10:20

... biosynthesis, and still in the range of the IC50 (0.03–4 nM) of type I RIPs The IC90 (the concentration of RIP causing 90% inhibition of translation) of lamjapin in the cell-free system of rabbit ... protein synthesis in the cell-free system of rabbit reticulocyte The protein synthesis decreased gradually with the increment of lamjapin in the reaction mixture The IC50 (the concentration of RIP ... CL-4B were then subjected to a general protocol of RIP puriﬁcation As Fig shown, the CM-cellulose 52 column retained the most of the RIP activity and also separated the lamjapin from the other impurities...

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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Ngày tải lên : 08/03/2014, 16:20

... isolated and sequenced the gene encoding the 21-kDa polypeptide Degenerate oligonucleotides designed from the sequences of N- and C-terminal peptides (P1 and P10) were used as primers to synthesize the ... X97910 The missing amino-acid stretches and the transit peptide sequence were deduced from the cloned cDNA and gene sequences (accession numbers: AJ304856 for the gene and AJ304857 for the cDNA) The ... the 2Cys-Prx group, because of the presence of two conserved cysteines [5,6] Arabidopsis BAS1 was shown to be a chloroplastic protein The identity of our peptides with BAS1 and the presence of...

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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Ngày tải lên : 16/03/2014, 18:20

... used for the quantiﬁcation of the enzymatically formed H2S Measurement of pyruvate formation The activity of the puriﬁed d-CDes enzyme was followed through the measurement of the amount of pyruvate ... counting from the start methionine was predicted, indicating a presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and ... dark cycle and the parts above ground were harvested every h and frozen in liquid nitrogen The d-CDes mRNA levels increased at the end of the light period, reached a maximum at the end of the dark...

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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

Ngày tải lên : 17/03/2014, 03:20

... upstream from the poly(A) of GT-cDNA1 and another is located 11 bp upstream from the poly(A) of GT-cDNA2 (data not shown) Of particular interest is the presence of eight AUUUA motifs in the 3¢-UTR of ... Organization of the gcGLUT gene (A) The 12 exons of the gcGLUT gene are shown as boxes Filled and open boxes indicate translated and untranslated regions, respectively The position of the start (ATG) and ... arrows (ﬂ) .The 5¢-end of exon was inferred by 5¢-RACE and the two alternate 3¢-ends of exon 12 were deduced by 3¢-RACE, and are delineated by the full-length cDNA clones, GT-cDNA1 and GT-cDNA The two...

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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

Ngày tải lên : 23/03/2014, 13:20

... form the membrane subcomplex, while the remaining four subunits (NdhH–K) form the connecting subcomplex, both of them homologous to the bacterial subcomplexes The theoretical molecular mass of the ... about the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge of the protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...

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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitriﬁcans docx

Ngày tải lên : 30/03/2014, 13:20

... after the addition of 0.4 mM NADH Fig N-Terminal amino acid sequences of P denitrificans FerA and FerB (A) and comparison of the N-terminal amino acid sequence of FerB with the sequences of other ... Sapshead, L.M & Wimpenny, J.W.T (1972) The influence of oxygen and nitrate on the formation of the cytochrome pigments of the aerobic and the anaerobic respiratory chain of Micrococcus denitrificans Biochim ... points of FerA and FerB as 6.9 and 5.5, respectively These values are in accord with the observed chromatographic behavior of the enzymes at the Sepharose Q step (iv) During optimization of the...

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Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt

Ngày tải lên : 07/08/2014, 18:21

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

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Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys docx

Ngày tải lên : 10/08/2014, 05:21

... we report the isolation of HPAI H5N1 from donkeys living in contact with diseased birds and demonstrate the presence of H5 seropositive ones in the neighbouring areas Methods Virus isolation ... usage that is similar to that of the seasonal human H1N1[31] Analysis of the NA gene revealed the presence of the 20-amino acid deletion (Fig 3) as well as the presence of amino-acid R at position ... antibiotic therapy and one shot of antipyretic (Diclofenac sodium) with no recorded mortalities The inhibition induced by antibiotic to the possible secondary bacterial invasion, besides the moderate...

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báo cáo khoa học: " Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)" ppt

Ngày tải lên : 12/08/2014, 03:21

... methyl halides, from these plants and to clarify the mechanism of synthesis of these compounds The emission rates of CH3I from these plants in the presence of mM iodide were 0.4 (T aestivum) , 3.1 ... encoded HTMT from R sativus HT performed the isolation and heterologous expression of the HTMT encoding gene from R sativus and characterization of the enzymatic properties of recom- Page of 10 (page ... ml/min of N2) by GC The retention times of CH3CN and CH3SCN were 1.48 and 4.48 min, respectively, and the products were quantified using the peak area To calibrate the concentrations of the products,...

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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx

Ngày tải lên : 12/08/2014, 03:21

... regulation of these promoters in peach and Arabidopsis Figure selected accuracy of the predicted expression patterns of Evaluation of the genes by RT-PCR Evaluation of the accuracy of the predicted ... corresponding to the regions upstream of the translation start codons of Ppbec1, Ppxero2 and Pptha1, respectively The sequences of these promoter regions as well as the cDNA of their corresponding ... by the interaction between promoters containing these types of cis-elements and orthologous transcription factors However, the identification and functional characterization of these types of...

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báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt

Ngày tải lên : 12/08/2014, 03:21

... characterization of promoters of carotenoid biosynthesis pathway genes will help understand the regulation of carotenoid biosynthesis pathway in tomato In this study, we describe the isolation and characterization ... one of the crucial enzymes for carotenoid biosynthesis Lycopene β-cyclase along with Lycopene ε-cyclase (LYC-E) bring about the cyclization of lycopene Activities of both of these enzymes together ... circadian elements and light responsive elements were found in the ShCYC-B promoter (Fig 2) A list of some of the relevant cis-elements detected and their relative positions from the translational...

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Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt

Ngày tải lên : 12/08/2014, 04:21

... using the neighbor-joining method ORF2 sequence of the interior this study method with Mega software Phylogenetic analysis based on the complete and evaluated using the isolate inbranch testand other ... interests The authors declare that they have no competing interests Authors' contributions All authors participated in the planning of the project XGH was the leader of the project WZ, SXY, QS, and ... analysis based on the complete ORF2 sequence of the isolate in this study and other 10 genotype HEV isolates in China, using the neighbor-joining method and evaluated using the interior branch...

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Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot

Ngày tải lên : 12/08/2014, 11:22

... for the identification and characterization of MPs in the sputa of CF patients Patients and Methods Study patients The study was approved by, and performed in accordance with, the ethical standards ... for MPs was in the initiation and amplification of the coagulation cascade and furthermore they play a pivotal role in thrombosis, in the propagation of inflammation, modulation of vascular tone, ... plasma membrane of the original cell at the precise moment of MPs production and thus allows the characterization of the cellular source [33] using antibodies directed against these specific epitopes...

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Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle" pps

Ngày tải lên : 12/08/2014, 18:22

... lesions of DD were reproduced in any of the animals in the challenge trial Isolates T 603 and T 657 are from the same herd that is geographically remote from the other herds in the study They have ... carried out the RAPD analysis and contributed to the evaluation of the results HH and MP adapted the PFGE protocol for the phylotype studied CB, HH, and KEJ participated in planning of the investigation ... RAPD The products from a Primer RAPD reaction are shown in Figure Reactions with isolate V1 and T 551 gave similar bands but some of the T 551 bands were weaker The patterns of isolates T 603 and...

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Isolation and characterization of allergens from curvularia lunata 1

Ngày tải lên : 14/09/2015, 08:47

... Objectives of the present study The purpose of this study is to identify and characterize the allergens from Curvularia lunata and to characterize these allergens and cross-compare it with other putative ... 22% in children of age group of 6-7 years, and 12% in those of age 12-15 years In the above study, demographics and socioeconomic factors influenced the prevalence and severity of allergic disorders ... better understanding of the repertoire of Curvularia allergens, and of the allergies caused by Curvularia This would then be useful in targeting various diagnostics as well as immunotherapy tools...

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Isolation and characterization of allergens from curvularia lunata 2

Ngày tải lên : 14/09/2015, 09:09

... software Briefly, the Cross_Match software compares a set of reads to a set of vector sequence and produces vectormasked versions of the reads screens and trims it The edited sequences are then ... inequalis) of the fungal species tested The 15kDa band showed the least binding frequency and was only seen in four of the tested species IgE binding patterns of the protein components suggest that the ... spectrometric analysis A total of bands were cut (Figure 2.6) Out of the bands cut, bands corresponded to the IgE binding bands from C lunata 1D western blots and bands 70 Chapter Figure 2.5: Western...

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