insulin igf signalling in embryonic and adult neural proliferation and differentiation in

Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Ngày tải lên : 11/09/2015, 10:14

... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial phase of neural induction, the ectoderm is induced ... Immunoflourescent staining of neural stem cell marker Nestin in SHH-CM and Control-CM treated EB Middle panel shows corresponding DAPI nuclear staining in blue and right panel shows corresponding merged ... pluripotency and proliferation 54! 4.5! Activation of SHH signaling in hESC during differentiation 58! v 4.6! SHH signaling influences lineage determination during spontaneous differentiation...

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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

Ngày tải lên : 20/02/2014, 02:21

... isoforms in hepatic insulin signalling, for the following reasons First, we found that the addition of insulin to rat hepatocytes did not increase DAG mass or change the Ó FEBS 2003 Insulin signalling ... glycogen synthase, in an insulindependent manner [49], supports the hypothesis of crosstalk between insulin and integrin -signalling pathways The lack of an insulin- elicited increase in DAG, shown here ... dexamethasone is known to retain cuboidal hepatocyte morphology in cultures and to in uence actin polymerization [48], the differences in insulin signalling (and also in EGF signalling) observed between...

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Báo cáo khoa học: Colocalization of insulin receptor and insulin receptor substrate-1 to caveolae in primary human adipocytes Cholesterol depletion blocks insulin signalling for metabolic and mitogenic control doc

Ngày tải lên : 23/03/2014, 12:20

... poorly to insulin, exhibiting insulin resistance that can be overcome by increasing concentrations of circulating insulin In the majority of cases it is still not known what defect in the insulin target ... that in each experiment insulin- stimulated phosphorylation of PKB was blocked Insulin- stimulated phosphorylation was obtained by setting the value with no insulin to 0% and at 100 nM insulin to ... receptors After incubating cells with a submaximal concentration of insulin the insulin- induced increase in receptor autophosphorylation was comparable in the isolated caveolae fraction and in the total...

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Báo cáo y học: "Long Term Persistence of IgE Anti-Influenza Virus Antibodies in Pediatric and Adult Serum Post Vaccination with Influenza Virus Vaccine"

Ngày tải lên : 25/10/2012, 11:10

... anti-Influenza virus antibodies in serum of IgE positive and negative vaccinated pediatric and adult subjects, approaching two years post vaccination The exact role of IgE in Influenza virus infection ... subjects Vaccine description All adults were vaccinated with Influenza Virus Vaccine Fluzone® (inactivated Influenza Virus Vaccine, 2009-2010 Formula; Sanofi Pasteur Inc., Swiftwater, PA) and children ... levels and IgE and IgG anti-Influenza virus antibodies were studied in children (N=3) (m/f 14-16 y/o) and adults (N=3) (m/f, 41-49 y/o) approaching two years post vaccination, as well as in non infected,...

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Tài liệu Báo cáo " Effect of Sweet potato (Ipomoea batatas (L.) Lam) leaf extract on hypoglycaemia, blood insulin secretion, and key carbohydrate metabolic enzymes in expermentally obese and STZ-induced diabetic mice " pptx

Ngày tải lên : 12/02/2014, 17:20

... fasting glucose and plasma insulin secretion in obese-diabetic mice Glucose (mmol/l) Treatment with extract fraction Starting point Final Change Plasma insulin (pmol/l) of glucose Starting point ... diabetic STZ induced HFD fed mice were expressed diabetic disease and a insulin resistance However, plasma insulin in HFDfed mice injected STZ was lower as compared to HFD mice without STZ injection ... Therefore, obesity and insulin resistance were the important causes of diabetes 3.3 Effect of the extract fractions on blood fasting glucose level and plasma insulin secretion in type 2-diabetic...

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Báo cáo khoa học: Identiﬁcation and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

Ngày tải lên : 08/03/2014, 08:20

... expected initiation methionine by translating the fusion protein GST-XN73 and His6-XN73 As can be seen in Fig 2B, these fusion proteins run at the expected height, proving that no internal methionine ... proteins from an in vitro transcribed RNA injected into X laevis oocytes High expression is obtained as a consequence of inserting the cDNAs between the 5¢- and 3¢-UTR of b-globin, a protein that ... gov/blast/) and the ÔMULTALINÕ alignment program (http:// prodes.toulouse.inra.fr/multalin/multalin.html) Human genome sequences were obtained by doing BLAST searches against the HTGS database and the...

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Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx

Ngày tải lên : 29/03/2014, 21:20

... relaxin-3 is considered to be different from that of other relaxin proteins involved in the growth and remodeling of reproductive and other tissues during pregnancy [10] In the mouse and rat brain, ... appetite and the HPG axis) This group includes NPY, orexin and galanin-like peptides [51–54] Conclusions In this minireview, relaxin-3, which is the latest member of the insulin ⁄ relaxin family, ... Endocrine Society) cumulative food intake in ad libitum-fed rats [32] After such chronic administration, the plasma concentration of leptin and insulin was signiﬁcantly increased [32] In addition...

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Báo cáo Y học: Lipid rafts and little caves Compartmentalized signalling in membrane microdomains pot

Ngày tải lên : 31/03/2014, 21:21

... active role in facilitating efﬁcient and speciﬁc signalling However, lipid rafts might also be involved in negatively regulating signals by sequestering signalling molecules in an inactive state ... may enhance TCR signalling by bringing positive signalling effectors into close proximity, while excluding negative signalling molecules [92] SMACs may also be important in integrating costimulatory ... factor-stimulated signalling pathways including EGF [10,54], PDGF [49,55], FGF-2 [53], and insulin [56,57], are concentrated within lipid rafts The EGFR and the PDGFR are enriched within lipid rafts in unstimulated...

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Báo cáo sinh học: " Hepatoprotection and neuroprotection induced by low doses of IGF-II in aging rats" doc

Ngày tải lên : 18/06/2014, 19:20

... [4,5] acting analogous to IGF- I in aging rats Consistent with previous findings, old rats showed hyperlipidemia and insulin resistance, increased brain and hepatic oxidative damage and reduced ... binding protein blocked), which included elimination of interference by IGF binding protein through excess IGF- I as well as acid alcohol (ALPCO Diagnostics, Windham, NH) The sensitivity of IGF- I ... significant increase of insulin levels (see Table 1) In contrast with IGF- I therapy, IGF- II treatment was not able to increase testosterone circulating levels and slightly reduced insulin levels...

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Báo cáo sinh học: " Liver mitochondrial dysfunction is reverted by insulin-like growth factor II (IGF-II) in aging rats" doc

Ngày tải lên : 18/06/2014, 22:20

... insulinlike growth factor I in mammalian brain aging Growth Horm IGF Res 2004, 14(Suppl A):S39-43 30 Tong M, Dong M, de la Monte SM: Brain insulinlike growth factor and neurotrophin resistance in ... cells during myelination Glia 2007, 55:632-641 33 Fernandez S, Fernandez AM, Lopez-Lopez C, Torres-Aleman I: Emerging roles of insulinlike growth factor-I in the adult brain Growth Horm IGF Res ... González-Barón S, CastillaCortázar I: Low doses of insulinlike growth factor I improve insulin resistance, lipid metabolism, and oxidative damage in aging rats Endocrinology 2008, 149(5):2433-2442 Puche JE,...

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o cáo hóa học:" Hepatoprotection and neuroprotection induced by low doses of IGF-II in aging rats" docx

Ngày tải lên : 20/06/2014, 04:20

... [4,5] acting analogous to IGF- I in aging rats Consistent with previous findings, old rats showed hyperlipidemia and insulin resistance, increased brain and hepatic oxidative damage and reduced ... binding protein blocked), which included elimination of interference by IGF binding protein through excess IGF- I as well as acid alcohol (ALPCO Diagnostics, Windham, NH) The sensitivity of IGF- I ... significant increase of insulin levels (see Table 1) In contrast with IGF- I therapy, IGF- II treatment was not able to increase testosterone circulating levels and slightly reduced insulin levels...

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báo cáo hóa học:" Liver mitochondrial dysfunction is reverted by insulin-like growth factor II (IGF-II) in aging rats" pot

Ngày tải lên : 20/06/2014, 04:20

... insulinlike growth factor I in mammalian brain aging Growth Horm IGF Res 2004, 14(Suppl A):S39-43 30 Tong M, Dong M, de la Monte SM: Brain insulinlike growth factor and neurotrophin resistance in ... cells during myelination Glia 2007, 55:632-641 33 Fernandez S, Fernandez AM, Lopez-Lopez C, Torres-Aleman I: Emerging roles of insulinlike growth factor-I in the adult brain Growth Horm IGF Res ... González-Barón S, CastillaCortázar I: Low doses of insulinlike growth factor I improve insulin resistance, lipid metabolism, and oxidative damage in aging rats Endocrinology 2008, 149(5):2433-2442 Puche JE,...

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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx

Ngày tải lên : 08/08/2014, 17:20

... serotonin reuptake inhibitors In HD, in brains of HD patients and after quinolinic acid striatal lesioning of adult brain the enhanced neurogenesis in the SVZ leads to the migration of neuroblasts and ... recovery, an interaction that may provide clinical benefit for NSC-based therapy Adult neurogenesis, neural stem cells and cellular therapy In the adult mammalian brain, including in humans, neurogenesis ... immune cells involved in neuroinflammation and release of pro-inflammatory substances would result in reduced neuroprotection and neuronal repair, and increased neurodegeneration, leading to neurodegenerative...

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Báo cáo y học: " Effects of disease modifying agents and dietary intervention on insulin resistance and dyslipidemia in inflammatory arthritis: a pilot study" pptx

Ngày tải lên : 09/08/2014, 06:22

... methotrexate Insulin resistance was estimated using the Homeostasis Model Assessment for Insulin Resistance (HOMA), using the following formula: insulin (µU/ml) × glucose (mmol/l)/22.5 Insulin sensitivity ... estimated using the Quantitative Insulin Sensitivity Check Index (QUICKI), using the following formula: 1/(log insulin [µU/ml]) × log glucose (mg/dl) In accordance with our findings in a recent ... that, in IA patients with insulin resistance and/ or dyslipidemia, the use of DMARD in combination with dietary intervention was associated with a marked improvement in insulin resistance and sensitivity,...

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Báo cáo y học: "Estimation of stature from the foot and its segments in a sub-adult female population of North India" pps

Ngày tải lên : 10/08/2014, 21:24

... estimation in a sub -adult population and hence the findings of the study cannot be compared per se In such a forensic investigation, inter-observer and intra-observer error play an important role in ... of the foot and its segments and estimate stature from these measurements using linear and multiple regression models in a sub -adult female population of North India The study is intended to ... there is a growth spurt and long bones continue to grow leading to an increase in stature untill the individual attains maturity Stature estimation in the case of sub-adults is even more difficult...

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báo cáo khoa học: "Compound double ileoileal and ileocecocolic intussusception caused by lipoma of the ileum in an adult patient: A case report" ppt

Ngày tải lên : 10/08/2014, 23:20

... the pain, nausea, and vomiting became persistent and more severe During his admission to surgery, plain abdominal films clearly demonstrated signs of intestinal obstruction, airfluid levels in his ... his appendix and cecum, subsequently intussuscepted into his ascending colon, resulting in a double ileoileal and ileocecocolic intussuception His cecum and about 30 cm of his terminal ileum were ... intussusceptions are distinguished by the site of the pathologic lead point In ileocolic intussusception the lead point is in the ileum, but in ileocecal-colic intussusception the lead point is in the ileocecal...

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Báo cáo y học: "TLR4 signalling in pulmonary stromal cells is critical for inflammation and immunity in the airways" pdf

Ngày tải lên : 12/08/2014, 14:20

... inflammation and the production of Th2 cytokines by mediastinal lymph node T cells Interestingly, in the same mice, the levels of instructing cytokines were severely impaired Interestingly, inhalation ... to act as a link between TRIF and TLR4, like Toll/IL-1R domain-containing adaptor protein (TIRAP) bridging MyD88 to TLR4 MyD88 and TIRAP are involved in early activation of NF-B and MAPK [16-19], ... is involved in TLR, but also in IRAK-1-mediated IL-1R and -18R signalling A role for IL-1 and IL-18 pathways in this response was excluded, as bronchoconstriction, inflammation, and protein leak...

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Báo cáo y học: " Wnt signalling in lung development and diseases" pot

Ngày tải lên : 12/08/2014, 16:20

... Wnt7b signalling [64] Apart from β-catenin and Wnt-s, mRNA of Fz-1, -2 and -7 and several intracellular signalling molecules including Tcf-1, -3, -4, Lef1, and secreted Fz related proteins (sFrp-1, ... general features of inflammatory diseases and evidence for Wnt regulated signalling in inflammation in the joint [34], we have addressed the potential involvement of Wnt signalling in inflammatory diseases ... [69], and Dkk [70] Since, generally, Wnt signalling retains cells in a low differentiation state, the role of Wnt signalling in adult tissue may not be immediately clear If we assume that the maintenance...

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Báo cáo khoa học: " Energy balance, leptin, NEFA and IGF-I plasma concentrations and resumption of post partum ovarian activity in swedish red and white breed cows" potx

Ngày tải lên : 12/08/2014, 18:22

... Roberts AJ, Nugent RA, Klindt J, Jenkins TG: Circulating insulinlike growth factor 1, insulinlike growth factor binding proteins, growth hormone, and resumption of oestrus in postpartum cows subjected ... mainly because of a low sensitivity of the antibody in the low range of leptin values, is as effective and reliable as an ovine-specific RIA in determining leptin plasma profiles in the bovine ... resumption in primiparous dairy cows, the aim of the present study was to characterize the energy balance (EB) and leptin, NEFA and insulin growth factorI (IGF- I) plasma levels in Swedish Red and White...

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Báo cáo y học: "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity" pps

Ngày tải lên : 14/08/2014, 08:20

... dissecting telencephalons and dissociating them into a near single-cell suspension in DMEM media Approximately 1-2 × 105 cells were plated into 6-well plates containing DMEM/F-12 (Gibco) and N-2 ... selenium-insulintransferrin-ethanolamine (SITE), 0.2 mg/ml linoleic acidbovine serum albumin (LA-BSA), 0.8 mg/ml BSA (all from Sigma), × chemically defined lipid concentrate (Gibco) and 55 μM ... (NCAM) and cadherin 11 (Cdh11), which are expressed in MSCs, were not expressed in MSC-like cells, and Nrcam, Pdgfra, CD24, and Cdh13 were expressed in MSC-like cells but not in MSCs One thousand...

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