in vitro follicle growth and maturation
Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
Ngày tải lên :
18/02/2014, 04:20
... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...
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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
Ngày tải lên :
17/03/2014, 03:20
... I (NF-I) required to facilitate initiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting in origin unwinding under conditions of altered ... containing the PyV replication origin [21], used with human DNA polymerase a-primase and JCV TAg Values in panels (A) and (B), columns 2–7 were corrected for background incorporation determined in ... a-primase Standard deviations from two to four experiments are indicated as error bars The experiments in (A) and (B) were performed in parallel replication origin (pJC389) and either recombinant human...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
Ngày tải lên :
23/03/2014, 07:20
... Fig In vitro transfection activity of cationic lipids in the absence of helper lipid(s) (A) and in the presence of DOPE (B) or DOPE and cholesterol (C), as measured in a murine skin cell line ... and maintained in serum medium (Dulbeccos modied Eagles medium supplemented with 10% fetal bovine serum, 50 unitsặmL)1 penicillin and 50 lgặmL)1 streptomycin) After incubation for h at 37 C in ... series by only a single amine in the polar headgroup, elicited transfection activity Addition of a second amine group and the subsequent increase in rM increased the Novel cytofectins for gene delivery...
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báo cáo hóa học: " In vitro anti-leishmanial and anti-fungal effects of new SbIII carboxylates" pot
Ngày tải lên :
21/06/2014, 02:20
... fungi, growth in media containing sample under test were determined by linear growth (mm) measuring, and percent inhibition of growth was calculated with reference to negative control using formula ... dissolved in acetic acid (100 mL), and a cold solution of amino acid (33.77 mM, i.e 2.53 g, g, 5.58 g, and 4.63 g of glycine, b-alanine, L-phenylalanine, and 4-aminobenzoic acid, respectively) in acetic ... organoantimony(III) difluorides containing Y,C,Y pincer type ligands using organotin(IV) fluorinating agents J Fluor Chem 129:167–172 Gebel T (1997) Arsenic and antimony: comparative approach on...
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Báo cáo hóa học: " Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies" pptx
Ngày tải lên :
21/06/2014, 17:20
... solution containing the rutin (120 ml) was added to this organic phase maintaining the agitation for a further 10 Then, the organic phase was injected into an aqueous phase (534 ml) containing Tween ... rutin-loaded nanocapsules and nanoemulsions, as aqueous intermediate or final systems to the development of nanomedicines containing rutin Both formulations presented an increase in the rutin ... Biosystems) containing ml of the sample (0.25 mg ml-1) was put into a glass test-tube containing 200 ml of dissolution medium This system was maintained under constant moderate stirring during all the...
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Báo cáo khoa học: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility" doc
Ngày tải lên :
08/08/2014, 23:22
... technique are now used routinely (Webber, 1987; Webber and Painter, in preparation) However, methods for assessing pollen viability in vitro and relating the results to seed set remain rudimentary The ... of pollen grains arising from a single clone or seedling) were stored at a pollen moisture content of < 8% and at -20 °C in evacuated containers (see Webber, 1987; Webber and Painter, preparation) ... varied and ranged from 1-5 yr In vitro in lot viability assays Germination Media type The procedure for germinating Douglas fir pollen initially followed the technique described by Ho and Sziklai...
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Báo cáo y học: "Functional characterization of Trip10 in cancer cell growth and survival" ppsx
Ngày tải lên :
10/08/2014, 05:21
... tumorigenesis and metastasis in mice inoculated with Trip10-overexpressing IMR-32 cells (Figure 4A) On the other hand, huntingtin increases cell death by promoting apoptosis Thus, high levels of huntingtin ... distributed in the cytosol of CP70 control cells (Figure immunostaining) Overexpression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and form perinuclear foci In contrast, ... Cdc42 is involved in migration; therefore, strong Cdc42 expression in IMR-32 cells may cause them to become more invasive, possibly explaining the enhanced in vitro colony formation and in vivo...
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Báo cáo y học: "Discovering and validating unknown phosphosites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools" doc
Ngày tải lên :
10/08/2014, 09:22
... cocktail containing serine/threonine phosphatase inhibitors and one containing tyrosine phosphatase inhibitors) was also used Protein kinases were digested with lysyl endopeptidase and trypsin and subsequently ... López et al.: Discovering and validating unknown phospho-sites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools Journal of Clinical Bioinformatics 2011 1:16 ... the amino acid sequence was inspected to look for indications of why these y/b-ions were missing Peptides lacking a C-terminal arginine or lysine residue, due to a C-terminal position in the...
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Báo cáo y học: "In vitro anti-inflammatory and anti-coagulant effects of antibiotics towards Platelet Activating Factor and thrombin." potx
Ngày tải lên :
11/08/2014, 03:20
... most prominent anti-PAF activity were clarithromycin, azithromycin, linezolid, amikacin and netilmicin, while in the case of rPRP were amikacin, azithromycin, tigecycline and clarithromycin These ... approximately In this case, the most potent antibiotics in the rank were netilmicin, azithromycin, amicacin and daptomycin, while again meropenem did not influence thrombin activity in WRPs Several combinations ... clarithromycin, azithromycin, linezolid, amicacin and netilmycin Other drugs studied such as meropenem and vancomycin, did not influence PAF activity in WRPs From all antibiotics tested, only vancomycin,...
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Báo cáo y học: "In vitro anti-inflammatory and anti-coagulant effects of antibiotics towards Platelet Activating Factor and thrombin" doc
Ngày tải lên :
11/08/2014, 06:23
... most prominent anti-PAF activity were clarithromycin, azithromycin, linezolid, amikacin and netilmicin, while in the case of rPRP were amikacin, azithromycin, tigecycline and clarithromycin These ... approximately In this case, the most potent antibiotics in the rank were netilmicin, azithromycin, amicacin and daptomycin, while again meropenem did not influence thrombin activity in WRPs Several combinations ... clarithromycin, azithromycin, linezolid, amicacin and netilmycin Other drugs studied such as meropenem and vancomycin, did not influence PAF activity in WRPs From all antibiotics tested, only vancomycin,...
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Báo cáo y học: "Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity" docx
Ngày tải lên :
13/08/2014, 05:22
... of two independently folded subunits, the N- and C-terminal domains (NTD and CTD) [4] The N-terminal domains of CAp24 are assembled into hexameric rings [5] and each hexameric ring is joined to ... in HIV strains Since mutation of Asp51 to alanine has shown to be critical for proper capsid formation and subsequent replication of the virus, we extended the above findings and examined amino ... Morphological analysis of in vitro assembled mutant CAp24 Morphological analysis of in vitro assembled mutant CAp24 proteins Mutant and wild type CAp24 proteins were induced for in vitro CAp24 tubular...
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beckerman & solimano (eds.) - crisis and dollarization in ecuador; stability, growth, and social equity (2002)
Ngày tải lên :
03/11/2014, 17:19
... paralysis of the international financial institutions based in Washington, helped bring about an economic meltdown manifested in very high inflation, a banking crisis, economic depression, and social ... balancing, redistribution, and rent-seeking In turn, Ecuador, like most Latin American countries, is a highly socially stratified country Wealthy people, elite landowners, and financial and industrial ... comprehensively.) Ecuador maintains intricate systems of revenue sharing between the central administration, provincial and municipal governments, and sev- CRISIS AND DOLLARIZATION IN ECUADOR 38 Figure...
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Roles of BNIPXL in regulating cell growth and morphology
Ngày tải lên :
16/09/2015, 08:31
... increasing number of Dbl homology (DH) domain-containing proteins were isolated In addition to the DH domain, these proteins also possessed a pleckstrin homology (PH) domain found in tandem and ... and characterized in this laboratory This unique protein domain is about 145 amino acids in length and was initially known to be conserved in two proteins: BCL2/adenovirus E1B 19kDa interacting ... domain (residues 615-644), which resembles the Class I Rhobinding motifs (found in the RhoA effectors PKN, rhophilin and rhotekin) and other neighboring regions abolished both RhoA binding and...
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MECHANISTIC CHARACTERISATION OF DENGUE VIRUS RNA DEPENDENT RNA POLYMERASE NON NUCLEOSIDE INHIBITOR BINDING POCKET THROUGH IN VITRO BIOCHEMICAL ASSAYS AND REVERSE GENETICS ANALYSES
Ngày tải lên :
02/10/2015, 17:14
... performance during in vitro enzyme activity studies This work contributes to understanding the biological function of residues lining the RdRp catalytic domain in DENV NS5 Gaining insight into specific ... both finger and palm domains resulting in the closed conformation of RdRp during de novo initiation This is to restrict access to the template binding channel thereby preventing dsRNA binding to ... are coloured in grey and the GDD motif are in red box The NLS and priming loop are marked with black arrow and indicated in yellow and pink respectively The amino acid residue numbering is labeled...
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effect of probiotics suplement in water on growth and survival rate of cobia larvae (rachycentron canadum)
Ngày tải lên :
18/11/2015, 22:22
... measure body length and to define daily length gain (DLG) and specific growth rate in TL (SGR) as the following formula DLG (mm/day) = (Lnitial – Linal)/t SGR = (Ln Lfinal – Ln Linitial)/t x100% ... for all great encouragement and kind help during 4.5 years in CAF Finally, I thank my family and all my friends who have supported and encouraged me to study and finish my course Abstract This ... on intensive larval husbandry and fingerling production of cobia showed that cobia fry’s lengths were ranged form 1.5 to 2.0 cm at 20 -22 dph Hassler and Rainville (1975) pointed out that growth...
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Household Enterprises in Vietnam: Survival, Growth, and Living Standards
Ngày tải lên :
26/04/2016, 07:38
... if it fell into one of the bottom three quintiles in 1993 and one of the bottom two quintiles in 1998 And define a household as affluent if it was in one of the top two quintiles in both years ... probably think of employment in NFHEs as playing a bridging role, providing an attractive alternative to farming, but less appealing than most wage-paying jobs The unexpected finding for Vietnam ... that fall at least two quintiles as "sinking stones." We find that sinking stones (who were more affluent to begin with) have reduced their involvement in NFHEs while shooting stars (who were poorer...
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Effects of cassava forage (manihot esculenta crantz) in diet on growth and methane production in cattle
Ngày tải lên :
04/05/2016, 08:23
... to increase digestibility and weight gain On the other hand, feed intakes in replacement diet of FCF had weight gain lower than DCF and ECF diets It could explain that FCF-20 diet had HCN intake ... 4.5) leaded to increase DM intake Crude protein intake ranged from 366 to 489 g/head/day, similar to Shane Gadberry’s standard (1996) weight gain was 453 g/head/day and demand CP intake with 430 ... g/head/day and from 1.35 to 3.6 mg/kg BW not influence to DM intake 17 In general, intakes of DM, OM, CP and ME increased with replacement of dried, ensiled and fresh cassava forage in diets 4.3.2...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
Ngày tải lên :
23/03/2014, 13:20
... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
Ngày tải lên :
19/06/2014, 08:20
... binding determinant of 3CDpro lies in 3Cpro, lower affinity binding determinants are located in the 3Dpol domain [27,28] We have recently shown that a mutation (3CproR84A/I86A) in the RNA binding ... [reviewed in [49]] The exact function of 3CDpro(3CproH40A) in virus maturation is not yet known Interestingly, both the RNA binding sequences in 3Cpro and the integrity of interface I in the 3Dpol ... amino acids R455/R456 at interface I in the 3Dpol domain are essential for the protein's stimulatory activity both in VPguridylylation in vitro [20,33] and in the stimulation of RNA synthesis in...
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báo cáo hóa học: " Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro" pot
Ngày tải lên :
19/06/2014, 22:20
... loss and infarct volumes after ischemic brain damage [4] and direct application of the recombinant cytokine results in an enhanced infarct volume [5] In traumatic brain injury, antibodies against ... neuronal survival in the CNS [10, 11] This has also been observed in axonal growth in the peripheral nervous system both in vivo following sciatic nerve injury [12, 13] and in vitro in adult dorsal ... IL-1β in this context include the induction of IL-6, tumor necrosis factor (TNF)-α and nitric oxide [53] and increased proliferation of macrophages [54] and astrocytes [55-57] in vitro and in vivo...
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