in situ dynamic characterization of energy storage and conversion systems

Báo cáo khoa học: Peroxiredoxins as cellular guardians in Sulfolobus solfataricus – characterization of Bcp1, Bcp3 and Bcp4 pot

Ngày tải lên : 07/03/2014, 05:20

... cloning of bcp1, bcp3 and bcp4 and the characterization of the recombinant products and, next, we investigate the possible role of the SSO0192 ⁄ SSO2416 system as an in vivo partner of Bcps in ... the quantiﬁcation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Weber K, Pringle JR & Osborn M (1972) Measurement of molecular weight ... Da and a theoretical pI of 7.65; bcp3 encodes a putative protein of 149 amino acids with a predicted molecular mass of 16 976.42 Da and a theoretical pI of 8.84 Comparison of the sequences of...

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báo cáo khoa học:" Recent technological developments: in situ histopathological interrogation of surgical tissues and resection margins" ppt

Ngày tải lên : 11/08/2014, 23:22

... measure of proficiency is gained in topographical histopathologic interpretation Local specificity and sensitivity in detecting areas of abnormality should be ascertained to develop skills of having ... informed choice of the state of the in situ epithelial margin taken when excising squamous cell carcinoma by having better information about the tumour margin This together with the ability of ... experience in the determination of surgical margins at operation and later comparison with frozen section and paraffin section margins "gold standard" We were able to obtain a sensitivity of 95% and...

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Báo cáo y học: "Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport" doc

Ngày tải lên : 10/08/2014, 05:21

... PiT1-binding immunoadhesin generated by fusing the aminoterminal residues of the GALV (SEATO strain) Env, comprising the signal peptide, the RBD and the proline-rich region, to the rFc tag, herein, ... and PiT2 ligands PiT1 staining exhibited a linear pattern with few stained cells following the myelinated fiber bundles corresponding to oligodendrocytes (Figure 3B) Basal ganglia staining: In ... understanding of the metabolism in the brain, as well as in others organs such as the heart, the liver or kidneys In addition, these new ligands could help a better understanding of the role of their...

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Cellular energy supply and aging in dairy cows characterization of different physiological states and impact of diet induced over condition

Ngày tải lên : 26/11/2015, 10:17

... processes in times of a positive EB in late lactation and the beginning of the dry period On a cellular level, alterations of nutrient and energy requirements of an individual coincide with changes of ... biogenesis in blood and in tissues during different stages of lactation in PP and MP dairy cows, and 3) To give an overview about TL and TL- shortening in dairy cows during different stages of lactation ... adipokines (e.g adiponectin, leptin, resistin, visfatin, apelin) allows AT to communicate with the liver, muscles, brain, reproductive- and other organs of the body Furthermore, adipokines and...

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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt

Ngày tải lên : 07/03/2014, 21:20

... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound ... role, such as in DNA replication, in living cells In contrast, mutants of AtRPA70b were viable but showed high sensitivity to UV and MMS, suggesting involvement of AtRPA70b in the repair of damaged ... region, and a third, DBD-C, in the C-terminal region DBD-A and DBD-B are the most important regions for binding of ssDNA, whereas DBD-C has only weak ssDNA-binding activity At the N-terminus of RPA70kDa...

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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

Ngày tải lên : 16/03/2014, 01:20

... motif of an adjacent subunit and thus contributes to stabilizing the intersubunit interface Indeed, the Arg297 and Gln304 side chains of the Ca8 helix make favorable interactions with the Glu422 and ... frequency varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, which consists of only the catalytic domain and dimerization motif (Protein databank...

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Báo cáo khoa học: Coexpression, puriﬁcation and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx

Ngày tải lên : 30/03/2014, 15:20

... reveals the presence of a conserved PLP-binding site, a lysine residue at position 629, at the C-terminus of the OraE protein [11] The binding of PLP to D-ornithine aminomutase was investigated by ... speciﬁcity of D-ornithine aminomutase is strict Discussion Fig Binding of PLP to recombinant measured by equilibrium dialysis D-ornithine aminomutase The genes encoding D-ornithine aminomutase, oraE and ... was obtained per litre of culture A puriﬁcation method based on chromatography on Q-Sepharose ion- Measurement of the binding of PLP to proteins The binding of coenzyme B6 to D-ornithine aminomutase...

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mirlin. statistics of energy levels and eigenfunctions in disordered systems

Ngày tải lên : 24/04/2014, 17:14

... (3.75) and (3.78) of the non-perturbative calculation in this regime Furthermore, in the 1/g order [which means keeping only linear in terms in (3.84) and neglecting !i in denominator of Eq (3.85)] ... was originally introduced to describe #uctuations of widths and heights of resonances in nuclear spectra [12] Recently, interest in properties of eigenfunctions in disordered and chaotic systems ... the energy levels statistics but also the statistical properties of wave functions are of considerable interest In the case of nuclear spectra, they determine #uctuations of widths and heights of...

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Báo cáo lâm nghiệp: "Analysis of some dynamic series of forest production and trends of forest economics in the Czech Republic" ppt

Ngày tải lên : 07/08/2014, 03:22

... product (GNP) and forestry’s share in it, more recently the share of the industry in the value added, indicators of employment and wages, etc The share of forestry in the creation of GNP in the Czech ... of time periods Measures of the dynamics of studied phenomena can be tested by means of elementary indicators – absolute increment (including mean and relative increments), mean coefficient of ... timber felling, felling intensity and total mean increment per forest stand area (CPP) are presented in Table with the development of timber felling being illustrated in Fig The composition of extracted...

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Báo cáo lâm nghiệp: " Decomposition dynamic of fine roots in a mixed forest of Cunninghamia lanceolata and Tsoongiodendron odorum in mid-subtropics" docx

Ngày tải lên : 08/08/2014, 01:22

... concentration, lignin content, ratio of lignin/N and ratio of lignin/P of the initial material were the best predictors of mass loss for roots of the Chinese fir; and initial lignin concentration ... lignin was combined into the high-resistant secondary compounds; and the increase in relative importance of lignin as a predictor of mass loss in the later phase may indicate that C is increasingly ... rate of mass loss (P < 0.05) The Fine root decomposition in a mixed forest 67 Figure Percentage of dry-matter remaining over time in decomposing fine roots of Chinese fir and Tsoong’s tree Bars indicate...

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Báo cáo y học: "Kinematic and dynamic gait compensations in a rat model of lumbar radiculopathy and the effects of tumor necrosis factor-alpha antagonis" ppsx

Ngày tải lên : 12/08/2014, 17:22

... DeLeo JA, Weinstein JN: Effects of interleukin-1 beta, interleukin-6, and tumor necrosis factor on sensitivity of dorsal root ganglion and peripheral receptive fields in rats Eur Spine J 2006, ... contributes to spinal cord synaptic plasticity and inflammatory pain: distinct role of TNF receptor subtypes and Pain 2011, 152:419-427 Sweitzer S, Martin D, DeLeo JA: Intrathecal interleukin-1 receptor ... upregulation in glia and neurons after peripheral nerve injury: studies in murine DRG and spinal cord Spine (Phila Pa 1976) 2004, 29:1082-1088 Shubayev VI, Myers RR: Axonal transport of TNF-alpha in painful...

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Design, management and control of energy storage DC nano grid

Ngày tải lên : 09/09/2015, 09:59

... of high energy density and high power density, resulting in a light-weight and compact energy storage system 1.3 1.3.1 Challenges in Energy Storage DC Nano-Grid Constraints of energy storage ... energy management for DC nano-grid of energy storage devices such that constraints of energy storage devices are complied, and 16 the energy storage devices are prepared in advance in terms of ... reshaping of power grid at distribution level (micro-grid) and the increasing penetration of renewable energy (wind, solar) are being expected to bring the advantages of: • ease and low cost of...

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Functional characterization of RNA editing and alternative splicing in the carboxyl terminus of cav 1 3 calcium channel

Ngày tải lên : 10/09/2015, 15:48

... Profile of editing in CaV1.3 IQ motif in different mouse brain regions Figure 2.7 Developmental profile of editing in CaV1.3 IQ motif in mouse and rat brains Figure 2.8 Membrane expression of ... enhancement in membrane trafficking of α1 subunit, associated with an increase in the number of ligand binding sites, current amplitude, faster activation and inactivation kinetics and a hyperpolarizing ... µg) and rat β2a (1.25 µg) and α2δ (1.25 µg), using 0.5 µl of PLUS Reagent and µl of Lipofectamine LTX (Invitrogen, Carlsbad, CA) Cells 32 were incubated in DNA-Lipofectamine LTX mixture for h in...

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characterization of signaling pathways and significance of the axon guidance molecule plexin b3 in glioma progression

Ngày tải lên : 11/09/2015, 09:11

... proteins such as talin, vinculin, and ERM (ezrin, radixin, moesin) actin-binding proteins, act as linker proteins to connect the cytoplasmic domains of integrins to the cytoskeleton, resulting in ... domains Additional domains that are present in semaphorins include PSI (plexin, semaphorin and integrin) domains, immunoglobulin (Ig)-like domains, thrombospondin domains and PDZ-domain binding ... by inhibiting integrin function in a neuropilin- and plexin-dependent manner (Serini et al., 2003) Both A and B plexins are capable of hampering integrin-based adhesion, leading to Rho-kinase independent...

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Characterization of CMX 13 and evaluation of efficacy and mechanisms of action in animal model of autoimmunity

Ngày tải lên : 12/09/2015, 09:55

... Annexin V- Fluorescein-isothiocyanate (FITC) /PI 49 VI Staining of Apoptotic Cells 2.3 CLINICAL AND HISTOLOGICAL STUDIES ON MRL-lpr/lpr MICE 2.3.1 52 Breeding Conditions and Examination for Clinical ... histological indices Hence CMX-13 appeared to be effective in attenuating the clinical and histological disease activity in the MRL-lpr/lpr mouse model of SLE In the final part of the thesis, we examined ... be the driving force for autoreactive B-cells in SLE resulting in abnormalities in cytokine production, and suppression of these autoreactive lymphocytes could result in inhibition of autoantibody...

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Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients

Ngày tải lên : 12/09/2015, 09:55

... binding studies Specific binding sites for the 7B2CT peptide in mice and rat brain tissues were identified Distribution of these binding sites in the brain and also correlation of these binding ... modulatory action in pain processing In brain, the opioid peptides (β-endorphin, dynorphins and enkephalins) are widely distributed whereas in spinal cord dynorphins are present mainly in interneurons ... physiological amino acids especially the nine pain-related amino acids (asparagine, aspartate, GABA, glutamate, glutamine, glycine, taurine, arginine and citrulline – NO markers) in the CSF of pregnant...

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Cloning and characterization of diamine oxidase and glutamate decarboxylase genes of mustard (brassica juncea) and their roles in shoot morphogenesis in vitro

Ngày tải lên : 12/09/2015, 10:19

... Lai Lai and Dr Yu Hao, for their help and encouragement during my staying in Singapore Their presence has created an enjoyable and productive working environment and made my staying in Singapore ... evidence showing that polyamines (PAs), including putrescine (Put), spermidine (Spd) and spermine (Spm), and its oxidative product, H2O2, are implicated in shoot morphogenesis in vitro and/ or somatic ... in siliques and rosette leaves, lower in flower and cauline leaves and lowest in stem and root (Soyka and Heyer, 1999) Unlike ADC1, ADC2 transcript was accumulated predominantly in siliques and...

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Cloning and characterization of diamine oxidase and glutamate decarboxylase genes of mustard (brassica juncea) and their roles in shoot morphogenesis in vitro

Ngày tải lên : 16/09/2015, 17:11

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The synthesis and application of energy storage materials derived from small molecules

Ngày tải lên : 30/09/2015, 10:13

... part of the battery system and is absolutely vital in the improving current energy conversion efficiency of batteries Due to the deterioration of current environment and increasing awareness of ... Kian Ping, who has offered me lots of guidance and support during my study and research period in NUS Besides, I’m also deeply inspired by Prof Loh’s enthusiasm in exploring cutting-edge scientific ... spectrum of TCB and PTCB Further FTIR experiment was conducted to investigate the infrared transmittance of the product As can be seen in Fig 2.6, the main absorptions of infrared light for TCB and...

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Children’s Health Deficits due to Diarrhoea: Effects of Water Supply and Sanitation Systems in Slums with Different Water Logging Conditions

Ngày tải lên : 05/09/2013, 10:15

... persistent and non-inundation) and types of inundation (rainy, stagnant-water, heavy-rainy, monsoon-flood and drainage-water inundations) Ten subdistricts were selected by integrating information ... defecating in open field Households with children defecating in room Households with children defecating in verandah Households with children defecating in latrine defecating in latrine Households ... LMK PDL PHS PSM NCD Hand washing with soap before eating Hand washing without soap before eating No hand washing before eating Hand washing after defecation with soap Hand washing after defecation...

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