... on the si-face ofthe flavin in FNRs [12]. The
single point energies ofthe flavin–tyrosine system in
different conformations were calculated. The arrange-
ment of lumiflavin and phenol with the ... inter-
action ofthe nicotinamide of substrate NADP
+
with
the isoalloxazine is modulated by the terminal tyrosine
(Y308 in pea FNR) [15,17,18]. During binding of
NADP
+
, the terminal tyrosine should ... the
aromatic interaction between the flavin, Y308 and the
nicotinamide of NADP
+
is precisely tuned by selecting
amino acids that face the other side ofthe tyrosine
phenol ring. The specific volumes of the...
... notions of decline inthe study
of the polis (Models of Decline inthe Study ofthe Polis), before outlining
ways in which this notion has influenced studies of ancient religious life
in this ... origins and the his-
tory of other religious groups inthe early Roman Empire. Inthe first chapter
of the book, it was proposed that the category of mission be abandoned alto-
gether. Neither ... of Decline inthe Study of Social-Religious Life). I
then provide evidence for the continuing vitality of civic life by using the
inscriptional evidence for small social-religious groups in the...
... total volume ofthe column
(% 12 min). However, as previously found with other
dissociated capsid proteins, the lack of elution of the
proteins can be explained by non-specific binding of the
denatured ... resides on the surface ofthe protein and makes no
obvious stabilizing interactions with other amino acid side-
chains, this part ofthe protein interacts with genomic RNA
[33,41,47]. Such interactions ... different from those of other simple icosahedral
viruses [13]. The main chain ofthe protein subunit folds
into a five stranded antiparallel b-sheet (strands bC–bG)
facing the interior ofthe phage particle,...
... productive binding of steroid substrates:
in the first, a (small) sub population of enzyme binds
directly the steroid inthe reactive conformation, with
the remainder ofthe enzyme binding the unreactive
conformation. ... unusual in having
two histidine residues (rather than the His-Asn pair
seen in OYE, MR and some ofthe other member
proteins; Fig. 1B) inthe active site for ligand and
substrate binding. For ... role inthe binding of phenolic compounds
in the active site [3,26]. Likewise, in MR the counter-
part residues His186 and Asn189 form key interactions
with reducing nicotinamide coenzyme and the...
... đbronectin-binding protein [8], the brain
protein a-synuclein together with the related proteins
b-synuclein and c-synuclein [9±12], a nd the brain protein
tau [13±16]. In addition to their role ... relies mainly on the
evidence outlined above.
In view ofthe c lose similarity ofthe ROA spectra of the
casein, synuclein and tau proteins shown in t his paper, we
have reproduced in F ig. 1 ... a
globular protein c ontaining a similar number o f residues, as
observed inthe caseins [3], synucleins [10], tau [14],
prothymosin a [7] and the đbronectin-binding protein [8],
and also in typical...
... Thus, we were
interested in isolating the cDNA of PO-CHH and
identifying the native CHH neuropeptide inthe ES,
after the recent report of CHH cDNA from the ES
[28]. Also, we examined the physiological ... decrease in CHH expres-
sion inthe ES is in proportion to that of CCAP
expression inthe same tissue, as described in Chung
et al. [47], but there is no change in CCAP of TG,
indicating the tissue-specific ... targeted into the specific sites in
which the nerve fibers ofthe PO innervate the anterior
ramifications. On the other hand, as a neurohormone,
PO-CHH may be released from the surface ofthe PO
and into...
... inthe case ofthe K137E mutant
of HSP22 (Fig. 4B); however, in this case, the intensity
of the band corresponding to the HSP22 dimer was
more intense than inthe case ofthe wild-type protein.
Thus, ... intact protein at the beginning of tryp-
sinolysis and at the fixed time of trypsinolysis) against
the time of incubation (Fig. 8D). The apparent rate
constants of trypsinolysis under these conditions ... shock proteins, each
of which contains the so-called a-crystallin domain consisting of 80–
100 residues and located inthe C-terminal part ofthe molecule. The
a-crystallin domain consists of six...
... attitudes toward the
intendedness of pregnancies based on
their own reports (13).
+ Of all births inthe 5 years before
interview, 55 percent were intended
by both the mother and the father
(as ... instead
of the full phrase, ‘‘not Hispanic white,
single race.’’
All characteristics of women reflect
the time of interview unless otherwise
indicated inthe tables. While most
tables in ...
80. Number of women 15–44 years of age who were working during the week prior to the interview and have at least
1 child under 13 years of age inthe household and percentage using the specified...
... produced
(Fig. 1D, lane 2). Inthe presence of lactacystin, a sig-
nificant increase inthe amount of tBid–GFP present in
the extract, both in control conditions and inthe pres-
ence of FLAG–Itch, was ... protein ofthe Bcl-2
family that is crucial for death receptor-mediated
apoptosis in many cell systems [16,17]. The BH3
domain-only proteins are a subfamily ofthe Bcl-2
family involved inthe initiation ... apoptosis is critical in the
maintenance of a healthy organism, and tipping the
equilibrium in one or another direction results in either
Keywords
apoptosis; Bid; c-Jun N-terminal kinase;
epidermal...
... ofthe P-loop main chain (starting at the
residue prior to the catalytic cysteine and ending after
the conserved arginine) resulted in rmsd values of
< 0.75 A
˚
(supplementary Table S3). In ... upon substrate
binding, due to the presence of a small b-barrel domain
that is unique to this protein and is involved in
substrate binding [7,8]. No other residues inthe protein
were found to ... [18]. Treatment of PhyAsr with
100 lm H
2
O
2
resulted in 33% ofthe enzyme being
inactivated after 10 min of treatment and 65% being
inactivated after 30 min. Incubation ofthe enzyme for
up...