human peripheral blood mononuclear cell culture for flow cytometric analysis of phosphorylated mitogen activated protein kinases
Báo cáo y học: " Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation" pps
Ngày tải lên :
13/08/2014, 05:20
... Franzese O, Balestrieri E, Comandini A, Forte G, Macchi B, Bonmassar E: Telomerase activity of human peripheral blood mononuclear cells in the course of HTLV type infection in vitro AIDS Res ... levels of PTHrP mRNA and protein expression were likely due to differences in translation efficiency, processing of the mature protein, and/or its secretion from the cells Regulation of Page of 12 ... KT: Human T -cell leukaemia virus type (HTLV-1) infectivity and cellular transformation Nat Rev Cancer 2007, 7:270-280 Yasunaga J, Matsuoka M: Human T -cell leukemia virus type I induces adult T-cell...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... 12.5 lM) for h prior to addition of SEC1 (1 ngÆmL)1) After 24 h of incubation, the supernatant fluids were collected for cytokine analysis and for the fever index of pyrogen test in rabbits For experiments, ... tested and revealed to be nontoxic to the cells) or the indicated inhibitors for h before treatment of SEC1 for 24 h (D) Analysis of EMSA shows the inhibition of SEC1-induced NF-jB activity by the ... toxic to the cells), ETYA (60 lM), MK 886 (10 lM), Wort (400 nM), or HIMO (25 lM) for h before treatment of SEC1 (1 ngÆmL)1) for 24 h Nuclear proteins were subjected to western blot analysis by...
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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc
Ngày tải lên :
30/03/2014, 04:20
... effectiveness of MK591 Experimental procedures Cells and cell cultures Effects of SEB on human PBMCs vitamin solution (Gibco BRL, Grand Island, NY, USA) The cultures were maintained for up to seven ... T (2001) Phosphorylation of p38 mitogenactivated protein kinase downstream of bax-caspase-3 pathway leads to cell death induced by high D-glucose in human endothelial cells Diabetes 50, 1472–1481 ... ngÆmL)1 of SEB for different time periods (1, 5, 30 and 60 for protein extraction and h and 16 h for RNA extraction), PBMCs were incubated with an inhibitor of interest at room temperature for 30...
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Báo cáo hóa học: "Safety evaluation of allogeneic umbilical cord blood mononuclear cell therapy for degenerative conditions" pot
Ngày tải lên :
18/06/2014, 16:20
... of allogeneic cord blood therapy in absence of immune suppression/myeloablation This study presents for the first time a detailed safety analysis of using non-matched, allogeneic cord blood cells ... accordingly Cells were harvested for clinical application after one week of cultivation with cell quantity ≥1 × 107 and viability ≥95% To ensure the quality of the UCB-derived mononuclear cells, a ... and were removed from the data analysis The SPSS 13.0 statistical package was applied for statistical analysis Results Administration of cord blood mononuclear cells via intrathecal and intravenous...
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Báo cáo y học: " A Novel Population of Mesenchymal Progenitors with Hematopoietic Potential Originated from CD14- Peripheral Blood Mononuclear Cell" potx
Ngày tải lên :
08/08/2014, 18:20
... cytometry Because CD14+ cells accounted for about one tenth of CD14- cells and the isolation was performed with a very small number of seeded cells We set 105 cells and 106 cells as one unit, respectively, ... have been unrewarding, except for a report of Fernandez et al, who identified cells with the features of MSCs in growth-factor-mobilized peripheral blood cells of breast cancer patients [13] ... obviously difficult and these cells are subject to variation depending on the methods for isolation and sorting of peripheral blood mononuclear cells (PBMCs), and on culture conditions [12-16] We...
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báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc
Ngày tải lên :
10/08/2014, 22:20
... HER-2 Cell Equivalents (x10) CD16 Antibody used for cell sorting 20 15 10 CD14 CD16 CD3/19 Total Antibody used for cell sorting Figure A-D Flow cytometry sorting of peripheral blood mononuclear cells ... immunomagnetic selection of peripheral blood mononuclear cells (PBMC) from CK19 and HER2 RNA expression from serial immunomagnetic selection of peripheral blood mononuclear cells (PBMC) from normal ... Antibody used for cell sorting CD3/19 Total 35 Cell number by B2M HER2 30 25 20 15 10 CD14 CD16 CD3/19 Antibody used for cell sorting total PBMC HER-2 Cell Equivalents /Cell (X 10 –4) H 40 B2M Cell Equivalents...
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Báo cáo y học: "Controlled meal frequency without caloric restriction alters peripheral blood mononuclear cell cytokine production" potx
Ngày tải lên :
11/08/2014, 03:20
... Peripheral Blood Mononuclear Cells In the absence of antigenic challenge, immune cells produce negligible or low levels of pro-inflammatory cytokines In an effort to understand the impact of meal ... consume the meal/d diet Separation and stimulation of peripheral blood mononuclear cells (PBMCs) The PBMCs were separated from fresh heparinized blood of healthy adult donors using Ficoll density gradient ... significance of difference observed in the two study groups Results Serum Markers of Inflammation Measurement of C-reactive protein (CRP), ICAM-1, VCAM-1 and soluble gp130 proteins in the peripheral...
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Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures" potx
Ngày tải lên :
12/08/2014, 15:21
... epithelial cells and white blood cells, we decided to investigate the role of the IFN-γ/IL-12 pathway on IP-10 secretion upon the interaction of peripheral blood mononuclear cells with two human lung ... Maintenance of human epithelial cell lines Cells from a human bronchial epithelial cell line (Calu-3) and from a human alveolar epithelial cell line (A549) were used for the present studies Both cell ... collected for IP-10 and IFN-γ ELISA analysis Isolation of lymphocytes from PBMCs After resuspension in 1% FCS cell media to × 106 white blood cells/ml (see above in Peripheral Blood Mononuclear Cell...
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Báo cáo y học: "Interferon-lambda1 induces peripheral blood mononuclear cell-derived chemokines secretion in patients with systemic lupus erythematosus: its correlation with disease activity" potx
Ngày tải lên :
12/08/2014, 15:23
... presence of IFN-l1 for 30 minutes before the addition of LPS at the doses indicated Cells were then incubated for 72 h before being assessed for levels of IP-10 (a), MIG (b) and IL-8 (c) in culture ... by human PBMC and dose-relationship of IFN-l1- induced chemokines by PBMC Human PBMC were cultured for 72 h in the presence of recombinant IFN-l1 and supernatants were examined for levels of ... experiment of observing the synergistic effect of IFN-l1 and LPS, whole PBMC were incubated in the presence of different concentration of IFN-l1 for 30 minutes before the addition of LPS Supernatants...
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Báo cáo sinh học: "ERK2 mitogen-activated protein kinases affect Ras-dependent cell signaling differentially" pps
Ngày tải lên :
06/08/2014, 18:21
... in cell growth and Ras-mediated transformation, we made knockdowns of both ERK isoforms in NIH 3T3 cells, either in a wild-type or in oncogenic H-RasQ61L background, and performed a colony-formation ... Alterations of the ratio of ERK1/ERK2 to MEK and to any scaffold protein within the cell could therefore affect the threshold properties of the system and contribute to some of the non-linear cellular ... Identification of a novel inhibitor of mitogen- activated protein kinase kinase J Biol Chem 1998, 273:18623-18632 43 Sebolt-Leopold JS, Herrera R: Targeting the mitogen- activated protein kinase...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
Ngày tải lên :
18/06/2014, 19:20
... characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational ... elucidate the role of these cells in the ontogeny, spread, and treatment of cancer Page of 20 Methods Cell Lines and Cell Culture Tumor cell lines were obtained from the American Type Culture Collection ... significant for its broad analysis of human MDSC generation by a range of different cancer types represented by human tumor cell lines MDSC generated by co -culture methods were then characterized for...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
Ngày tải lên :
19/06/2014, 08:20
... chimeric anti-tumor T cells, forming basis for immunotherapy of many different human cancers Mouse cells and human lymphocytes MD45 cells, a murine cell NK-like T cell line, were obtained from ... presence of cell culture medium alone was considered background The results were interpreted as specific redirected signaling of peripheral blood lymphocytes by the chTCR Regression of tumors ... chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve the responses of T-bodies in...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
Ngày tải lên :
20/06/2014, 04:20
... chimeric anti-tumor T cells, forming basis for immunotherapy of many different human cancers Mouse cells and human lymphocytes MD45 cells, a murine cell NK-like T cell line, were obtained from ... presence of cell culture medium alone was considered background The results were interpreted as specific redirected signaling of peripheral blood lymphocytes by the chTCR Regression of tumors ... chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve the responses of T-bodies in...
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Báo cáo y học: "Gene expression signatures for autoimmune disease in peripheral blood mononuclear cells" potx
Ngày tải lên :
09/08/2014, 01:23
... studies of longitudinal responses to a therapeutic intervention [13] While peripheral blood offers many advantages as a source of analysis material, one potential drawback is the small quantities of ... Surprisingly, information about the amount of blood needed to produce an analyzable sample has not been uniformly reported; one group used lymphocytopheresis, suggesting a need for large numbers of cells ... shipping of the samples [15], although the advent of RNA stabilization tubes for blood collection may make this less of a concern in future studies Studies of the normal immune response Most sudies of...
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Báo cáo y học: "Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy" doc
Ngày tải lên :
13/08/2014, 06:20
... changes in peripheral blood monocytes during progression of human immunodeficiency virus infection Effects of soluble immune complexes, cytokines, subcellular particulates from apoptotic cells, and ... that modulation of cell surface markers occurs during the course of HIV disease and following the initiation of HAART and these cell surface markers may indicate the outcome of antiretroviral ... expression of Page of 12 (page number not for citation purposes) Retrovirology 2008, 5:24 http://www.retrovirology.com/content/5/1/24 Figure Composite dot scan patterns of antibody binding for PBMC cells...
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Báo cáo y học: " Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells" pdf
Ngày tải lên :
13/08/2014, 09:21
... tRNAPro in human peripheral blood mononuclear cells (PBMC) Infections were initiated with transfection supernatant containing approximately 200 pg of p24 antigen in a volume of 10 mLs of media, ... with re-feeding of PBMC every 14 days for periods of time exceeding 50 days of in vitro culture The cultures were sampled periodically, supernatants were assayed for p24 antigen and cells were processed ... tRNAMet remained stable for use of tRNAMet throughout the complete culture period (in this case, up to 63 days post initiation of culture) Analysis of 34 individual TA clones of the PBS from these...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
Ngày tải lên :
14/08/2014, 18:20
... moDCs and KG-1 cells refereed research Figure Cell culture work -flow Cell culture work -flow Overview of the methods used for isolation/generation of mDCs, moDCs and DC-like KG-1 cells deposited ... that peripheral blood mDCs have sixand five-fold lower protein content per cell than moDCs and KG-1 cells, respectively (data not shown) Unfortunately, the low numbers of mDCs in peripheral blood ... 3% of peripheral blood mononuclear cells (PBMCs) This has, therefore, prompted researchers to use other model systems for studying mDC biology For instance, DCs can be generated in vitro from peripheral...
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báo cáo hóa học:" Combined intermittent hypoxia and surface muscle electrostimulation as a method to increase peripheral blood progenitor cell concentration" pot
Ngày tải lên :
18/06/2014, 15:20
... of 4°C and processed within 24 h of arrival at the laboratory Blood cell count was assessed by use of an automatic cell counter (AcT-diff; Beckman Coulter, Miami, FL) Samples were incubated for ... Subsequently, blood samples were always obtained just before each of the experimental sets (OH, HME and OME) and 24 h, 48 h, Page of (page number not for citation purposes) Journal of Translational ... the accuracy of the assay by verifying the proportion of both types of beads Known volumes (25 μl) of Perfect-Count Microspheres were added to the same known volume (25 μl) of stained blood in a...
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