hts assay for detection of in vitro transcription reaction product by anti rna dna antibodies

Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Ngày tải lên : 25/10/2012, 11:10

... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase ... amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added during the ... prepared by diluting of packed cells of each type with 1.5 ml of distilled water Osmotic fragility was studied for each drug concentration In vitro releasing study The release of pravastatin as...

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Báo cáo khoa học: Identiﬁcation of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf

Ngày tải lên : 29/03/2014, 21:20

... cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) Such concerted reactions result in formation of ... +1 Interestingly, the amino acid located at this position is not important for the reaction in other Tgases, including TGase In addition, at the N-terminal side of the glutamine residue including ... inhibitor of cathepsin V and the short chain form of cathepsin L by a reactive site that is distinct from the legumain-binding site A novel clue for the role of cystatin M ⁄ E in epidermal corniﬁcation...

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STUDY ON EPIDEMIOLOGICAL FEATURES, APPLICATION OF DIAGNOSTIC KIT FOR DETECTION OF TRYPANOSOMIASIS CAUSED BY TRYPANOSOMA EVANSI IN CATTLE AND BUFFALOES IN A FEW NORTHERN MOUNTAINOUS PROVINCES AND RECOMMENDATION FOR PREVENTIVE AND TREATMENT MEASURES

Ngày tải lên : 28/04/2014, 13:08

... provinces by using PCR method (Polymerase Chain Reaction) - Method of study of clinical pathological features: + Determination of number of trypanosomes/ml blood of white mice by using neubauer counting ... X Indication Numbe Number of Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring ... X Indication Numbe Number of Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring...

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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc

Ngày tải lên : 18/06/2014, 15:20

... (in a single assay) Intra assay precision is determined by % CV (coefficient of variation) as (SD/ Mean) × 100 tested multiple times by one analyst in a single assay Inter assay precision (Intermediate ... RT-PCR assay was examined for assay accuracy and precision by spiking 1000 copies of IFNγ plasmid per sample in 80 repeats (n = 80) for intra -assay and 18 repeats (n = 18) for inter -assay performance ... optimal binding and to assure longitudinal assay performance Tetramer binding temperature (room temperature-RT or Page of 25 (page number not for citation purposes) Journal of Translational Medicine...

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báo cáo hóa học:" Evaluation of the tuberculin skin test and the interferon-γ release assay for TB screening in French healthcare workers" ppt

Ngày tải lên : 20/06/2014, 00:20

... high cut-offs for TST, a number of European Guidelines on the use of IGRAs suggest use of a two-step strategy - performing an IGRA in those initially positive by the TST and excluding LTBI in those ... especially combining the QFT assay following the TST screening of close contacts at a cut-off induration size of mm before LTBI treatment, is highly cost-effective in reducing the disease burden of TB ... tuberculin skin test for contact investigation of tuberculosis in BCG-vaccinated health care workers Scandinavian Journal of Infectious Disease 2008, 40(5):373-80 Schablon A, Beckmann G, Harling...

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Báo cáo hóa học: " Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies" docx

Ngày tải lên : 20/06/2014, 02:20

... there is only one type of infectious and replicating HCV The variety of HCV RNA in patients could result from changes induced in the plasma/sera, including the production of noninfectious viruses ... same thing as infectious virus Similarly, in the case of HTLV-1 and HTLV-2, the virus production in the plasma/sera of patients remains low [41] Despite best efforts, in vitro production of HTLV-1 ... to new cells of Impact of long-term in vitro cell culture on the fidelity of replication of HCV We tested the impact of long-term in vitro culturing on CIMM-HCV sequences Samples of PCLB-T4a and...

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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps

Ngày tải lên : 09/08/2014, 14:22

... antibodies by Micro Immunofluorescence assay as described by Wang and Grayston [29] bSerology was determined for Ct-IgA antibodies by ELISA (Labsystems, Hilsinki, Finland) cChlamydia PCR in genital ... amplification of 16S rRNA genes, cloning and sequencing of bacterial DNA For 10 minutes, 500 μl of SF were centrifuged at 10,000 g The whole SF cell pellet was subjected to DNA extraction using the ... 10:R40 Wilkinson NZ, Kingsley GH, Jones HW, Sieper J, Braun J, Ward ME: The detection of DNA from a range of bacterial species in the joints of patients with a variety of arthritides using a nested,...

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Báo cáo y học: "Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid, moderate throughput assay for joining activity in solution" ppt

Ngày tải lên : 10/08/2014, 05:21

... Moderate-throughput solution assay for integrase joining Moderate-throughput solution assay for integrase joining activity Panel A Principles of a solution assay to measure integrase joining activity by fluorescence ... employed by Landgraf et al [35] in development of a quantitative assay for PCR products The advantage of having biotin on both strands of the target DNA is that products of joining to either target DNA ... of HIV-1 IN inhibitors that are effective against ASV IN We were also interested in evaluating the utility of the fluorescent assay for determining IC50 values for integrase inhibitors In this...

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Báo cáo y học: "Threshold for detection of diabetic peripheral sensory neuropathy using a range of research grade monofilaments in persons with Type 2 diabetes mellitu" pot

Ngày tải lên : 10/08/2014, 21:23

... protocol used in the study) Currently, the cost of an individual monofilament manufactured by Bailey Instruments Manchester is £14 plus VAT, therefore setting the cost of a series of monofilaments ... the feet This would include the introduction of daily inspection of feet and discussion relating to the appropriateness and necessity of having correctly fitting footwear, introduced to the person ... 10-gram) of £42 plus VAT against the mean weekly cost of £59 for a non-infected foot ulcer [28,29] clearly demonstrating the potential cost-effectiveness of a range of monofilaments The results of...

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báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc

Ngày tải lên : 10/08/2014, 22:20

... of detecting CTC will prove to be extremely useful tools for clinicians in diagnosing breast cancers, determining prognosis and monitoring treatment responses More effort should be invested in ... μL of RNA were added to the unit dose vial Thermal cycling conditions were as follows: incubation at 60° for 60 minutes, then 94° for 40 seconds and 58° for minute for 45 cycles After cycling ... dilutions of breast cancer cell The sensitivity of the CK19 assay, as tested by dilutions of the cell line RNAs, was approximately 0.01 ce for each cell line (data not shown) Using this method in healthy...

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Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps

Ngày tải lên : 12/08/2014, 04:21

... GPV In this study, we report the development of LAMP assay for the specific, rapid, and sensitive detection of GPV in infected goslings Results Optimized LAMP reaction LAMP reaction was performed ... goslings Optimal LAMP assay was evaluated by analyzing GPV infected gosling tissues DNA extracted by the tissue Figure Determination of the optimal temperature and time of LAMP (A) Determination ... a finding confirmed by our results In other words, the specificity and the detection limit of the LAMP assay are equal with FQPCR for detecting GPV The optimal condition for detecting GPV by...

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Báo cáo khoa học: " Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos" pps

Ngày tải lên : 12/08/2014, 04:21

... distribution of avian infectious bronchitis virus following in ovo inoculation of chicken embryos examined by in situ hybridization with antisense digoxigenin-labeled universal riboprobe J Vet Diag Invest ... described by [20] These differences in sensitivity may be due to larger number of antigenic epitopes recognized by the polyclonal serum This finding prompted us to apply IHC for screening of antigen ... kidney, skin, sclera of the eye, spinal cord, as well as in neurons of the central nervous system in infected embryos However, isolation or detection of virus by virological methods may indicate...

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Báo cáo khoa học: "Evaluation of a commercial Erns-capture ELISA for detection of BVDV in routine diagnostic cattle serum samples" pdf

Ngày tải lên : 12/08/2014, 18:21

... Detection of antibodies The antibody titres of the sera from the nine PI calves were determined using the commercial indirect ELISA in dilutions 1:10 to 1:1250 The ELISA was performed according ... decline of bovine virus diarrhoea virus (BVDV) maternal antibodies and detectability of BVDV in persistently infected calves Acta Vet Scand 1993, 34(1):105-107 Brinkhof J, Zimmer G, Westenbrink ... comparison to IPX, for routine diagnostic detection of BVDV within a control programme In addition, the effect of passive immunity and heat-inactivation of the samples on the performance of the ELISA...

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Báo cáo khoa học: "Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes" pptx

Ngày tải lên : 12/08/2014, 18:22

... the opening of the tank for minutes and then at 13 cm for minutes and finally at 20 cm below the opening for minute, before plunging them into the liquid nitrogen The straws were thawed in a water ... decreased binding to FT ZP In line with this argumentation, such decreased binding capacity of the ZP may reside in changes of the structure of the ZP that occur during unprotected freezing and/ ... penetration Proceedings of the International Congress of Animal Reproduction and Artificial Insemination 1988, 1:244 Tsunoda Y, Chang MC: In vitro fertilization of hamster eggs by ejaculated or...

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Báo cáo khoa học: " Evaluation of three commercial bovine ELISA kits for detection of antibodies against Alphaherpesviruses in reindeer (Rangifer tarandus tarandus)" pdf

Ngày tải lên : 12/08/2014, 18:22

... Figure of three serological kits for detecting antibodies against alphaherpesvirus in reindeer by the ranks of the results Comparison Comparison of three serological kits for detecting antibodies ... conjugated anti- bovine IgG monoclonal antibodies) could not be used, as they would not recognize reindeer antibodies They were therefore replaced by a biotin labeled rabbitanti-reindeer antibody in a ... kits for detecting antibodies against BoHV-1 in cattle were evaluated regarding their ability to detect antibodies against alphaherpesviruses in reindeer: one indirect ELISA with BoHV-1 as antigen,...

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Báo cáo y học: "Cerebral microdialysis for detection of bacterial meningitis in aneurysmal subarachnoid hemorrhage patients: a cohort study" pptx

Ngày tải lên : 13/08/2014, 11:23

... pathophysiological insight in meningitis-related changes in brain metabolism, but also to facilitate diagnosis of BM in patients undergoing MD monitoring for the detection of symptomatic vasospasm, and thereby ... demonstrated by Schade and colleagues [3] In a study investigating the value of routine CSF analysis for diagnosis of BM in patients with external drains, it was not possible to establish a cutoff value ... measures in the beginning of immunoreactive cascades and thereby improve outcome in these patients [7] For investigating intracranial infections, the MD technique has only been used in animal...

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Báo cáo y học: "First successful case of in vitro fertilization-embryo transfer with venom immunotherapy for hymenoptera sting allergy" ppsx

Ngày tải lên : 13/08/2014, 13:22

... karyotypes obtained from both partners Anticardiolipin, antiphospholipid and antiovarian antibody titres were all negative However, transvaginal saline uterine sonography revealed a uniform mm echodense ... of IgG4 blocking antibody were not quantified in this study, although measurement of this parameter in subsequent studies may offer additional insight into potential mechanisms of protection ... Ching W: A rational basis for the use of combined heparin/ aspirin and IVIG immunotherapy in the treatment of recurrent IVF failure associated with antiphospholipid antibodies Am J Reprod Immunol...

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Báo cáo sinh học: "Comparison of four statistical methods for detection of a major gene in a progeny test design" pptx

Ngày tải lên : 14/08/2014, 20:20

... interest in new techniques for detection of single genes Various tests have been described concerning livestock (Hanset, 1982) Their general principle is that the within family distribution of ... sire, the increase of the quantiles is nearly linear with n (number of sires) allowing some extrapolations for higher values of this number - Finally, the jackknife standard deviation of the estimated ... heritability of 0.2 = Comparisons of the powers By using the table of the critical regions thus obtained for each family structure, powers depend not only on the number and size of the families in the...

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Báo cáo sinh học alignminer a web based tool for detection of divergent regions in multiple sequence alignments of c

Ngày tải lên : 20/12/2015, 08:16

... Page 14 of 16 Figure Analysis of two Pinus pinaster gene isoforms The specific primer pair for the photosynthetic isoform is identified by a “P” and for the non-photosynthetic isoform by an “N” ... the main motivation for development of AlignMiner: this software was intended to cover the gap in bioinformatic function by evaluating divergence, rather than similarity, in alignments that involve ... template in the different lanes is: cDNA for the photosynthetic isoform (lanes 1), cDNA for the non-photosynthetic isoform (lanes 2), cDNA synthesised from total mRNA extracted from Pinus pinaster...

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Tài liệu FUZZY CLUSTERING ALGORITHMS ON LANDSAT IMAGES FOR DETECTION OF WASTE AREAS: A COMPARISON pdf

Ngày tải lên : 16/01/2014, 16:33

... parameter, obtaining in such a way continuous membership values of patterns to classes The Deterministic Annealing (DA) is a diﬀerent fuzzy approach to clustering based on the minimization of a Free ... values (annealing procedure) It is worth of noting an on-line version of FCM, introducing also a scheduling of the fuzziﬁer parameter, has been recently proposed with the names of FKCN [10] and ... matrix, containing the membership values of all samples in all clusters; • m ∈ (1, ∞) is a control parameter of fuzziness The minimization of Jm , under the probabilistic constraint iteration of the...

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