hostpathogen interactions in sepsis
Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx
Ngày tải lên :
14/02/2014, 19:20
... blocking or presenting of
binding sites for the ribosome [4]. These are just a few
Keywords
mode of binding; proteins that promote
RNA folding; RNA chaperones; RNA folding
problem; transient interactions
Correspondence
B. ... changes in the RNA are
introduced during the transient interaction arises. Ini-
tial results indicate that the protein acts as an electro-
static lubricant that shields repulsive interactions
within ... helicases. The binding sites for double-
stranded RNA and ATP overlap, resulting in coupled
binding of both molecules. Simultaneous binding
forces the RNA into a bent conformation that is
incompatible...
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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc
Ngày tải lên :
16/02/2014, 15:20
... defense
mechanisms.
In addition to sepsis, there is an increasing number
of emerging infectious diseases in which the severe gen-
eralized in ammatory reaction of the infected host is a
major factor in the ... the
antigen-binding site and the involvement of hydropho-
bic interactions as a driving force for the recognition
of the target antigen. All of these findings allow us to
hypothesize that elevated binding ... of hydrophobicity in the binding
of a pH 4.0 buffer-treated monoclonal IgG
antibody to its target antigen
To evaluate the types of intermolecular interactions in
antigen binding, pH and salt concentration...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
Ngày tải lên :
18/02/2014, 08:20
... structure of the cobalamin-binding domain
Homocysteine
Methionine
Co
Methylcob(III)alamin
Cob(I)alamin
Primary
turnover
cycle
H
CH
3
H
4
folate
AdoHyc
Cob(I)alamin
Cob(II)alamin
H
4
folate
Co
NADPH
A
doMet
e
–
FAD
FMN
NADPH
NADP
+
e
–
Reactivation
Co
Fig. ... 53–56.
34 Tollin G (1995) Use of flavin photochemistry to probe
intraprotein and interprotein electron transfer mecha-
nisms. J Bioenerg Biomembr 27, 303–309.
Supporting information
The following supplementary ... CH
3
-H
4
-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark grey circle).
hMS is inactivated approximately every 200–1000 catalytic turnovers [owing to...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
Ngày tải lên :
19/02/2014, 06:20
... strong, neutralizing action of the cytosolic RNase
inhibitor (cRI), a 50-kDa protein containing 16 leu-
cine-rich repeat motifs [7,8]. In fact, onconase from
Rana pipiens eggs [9], and seminal RNase ... proteins, with high
isoelectric point values. Thus we investigated the effects
Table 1. Natural and engineered RNases investigated in this study. IC
50
is the RNase concentration producing half-maximal ... homologous to the RNases investigated in
this study, have been isolated from human eosinophils,
namely eosinophil-derived neurotoxin (EDN) and eosi-
nophil cationic protein (ECP). They are RNases...
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Managing Drug Interactions in the Treatment of HIV-Related Tuberculosis docx
Ngày tải lên :
06/03/2014, 04:20
... Gerstoft J, Pedersen C. Pharmacokinetic
interaction between rifampin and the combination of indinavir and low-dose ritonavir in HIV-
infected patients.
Clin Infect Dis.
Feb 1 2004;38(3):426-429.
26. ... together
Delavirdine AUC
by 95%
Etravirine
Etravirine and rifampin should not be used together
Marked decrease in etravirine predicted, based on data on the
interaction with rifabutin
Single protease inhibitors
Recommended ... Increasing nevirapine dose can
overcome reduced bioavailability due to rifampicin coadministration.
J Acquir Immune Defi c Syndr.
May 2006;42(1):36-41.
6
Predicting Drug Interactions Involving...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
Ngày tải lên :
08/03/2014, 09:20
... living cells (Eur. J. Biochem. 269) 5077
Probing intermolecular protein–protein interactions in the
calcium-sensing receptor homodimer using bioluminescence
resonance energy transfer (BRET)
Anders ... agonist-induced
alterations in BRET signal intensities reflecting 7TM dimer
rearrangement as a result of the activating twist in the ATDs
of the CaR homodimer. Further investigations into the
signal ... AT
1a
Rand
AT1aD359-GFP
2
displayed similar binding characteristics
in a [I
125
]angiotensin II whole cell binding assay (data not
shown). These observations are in excellent agreement with
the findings of another group...
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Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot
Ngày tải lên :
08/03/2014, 10:20
... overlay
method in determining protein linkage. Its greatest advant-
age is the direct visualization of protein–protein interactions
under controlled conditions. In contrast, other established
protein biochemical ... reduction in
calsequestrin binding to these sarcoplasmic reticulum ele-
ments. The calsequestrin-binding protein junctin was not
affected by the muscle transformation process. The increase
in calsequestrin ... protein–protein interactions.
Slow CSQ might be involved in a more indirect type of
physiological coupling process in transformed fibers, while
fast CSQ appears to be a directly interacting endogenous
regulator...
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Báo cáo khoa học: Nature, nurture and neurology: gene–environment interactions in neurodegenerative disease docx
Ngày tải lên :
16/03/2014, 19:20
... expansions, encoding
polyglutamine tracts in different proteins, suggesting
that these diseases may involve overlapping molecular
mechanisms of pathogenesis involving toxic gain-
of-function of ... progress has been made
in identifying genetic factors contributing to PD in
recent years [135]. Low concordance for clinical disease
in monozygotic twins indicates environmental in uen-
ces on PD ... (2002) Increased
huntingtin protein length reduces the number of
polyglutamine-induced gene expression changes in
mouse models of Huntington’s disease. Hum Mol Genet
11, 1939–1951.
72 Cha JH, Kosinski...
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Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot
Ngày tải lên :
16/03/2014, 22:20
... it is interesting that the guanine amino
group may be in uenced by this contact. We com-
pared the interaction energies in both a planar and a
nonplanar guanine G9 amino group. The HF interac-
tion ... an
inverted central tetranucleotide and its netropsin
complex. Acta Cryst D51 , 190–198.
17 Abrescia NGA, Malinina L & Subirana JA (1999)
Stacking interaction of guanine with netropsin in ... with the minor groove binding drug netrop-
sin. Biochemistry 36, 4792–4799.
13 Patel DJ (1982) Antibiotic–DNA interactions – inter-
molecular nuclear Overhauser effects in the netropsin-
d(CGCGAATTCGCG)...
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Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf
Ngày tải lên :
30/03/2014, 04:20
... reaction.
Abbreviations
APPBP1, APP binding protein 1; CtBP, C-terminus binding protein; CTD, C-terminal domain; E1, activating enzyme; E2, conjugating enzyme;
E3, ligase; HECT, homologous to E6AP C-terminus; IR, internal ... in
Nedd8, and Arg72 in ubiquitin (highlighted in yellow
in Fig. 2C). Matching E1 residues are Thr149 in
uba2, Arg190 in uba3 and Gln608 in uba1 (high-
lighted in purple in Fig. 3E). Interestingly, ... motif (SIM) and a C-terminal domain (CTD) [39]. (B) Pc2
contains an uncharacterized E3 domain, a ubc9 binding domain and
a Pro-Ile-Asp-Leu-Ser motif involved in substrate binding [53].
(C) RanBP2,...
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Báo cáo khoa học: Probing protein–chromophore interactions in Cph1 phytochrome by mutagenesis potx
Ngày tải lên :
30/03/2014, 11:20
... heterologously
expressed as C-terminally His6-tagged apoproteins in
E. coli, purified and tested for PCB-binding, apopro-
tein folding, Pr–Pfr photochromicity and thermal
reversion using SDS ⁄ PAGE ⁄ zinc fluorescence ... other protein–
bilin interactions, as studies with blocking reagents and
of autoassembly kinetics have implied [15–17]. In free
PCB at neutral pH 7 the two central ring nitrogens
share a single proton, ... mod-
ule (the N-terminal chromophore binding PAS ⁄ GAF
domains), the missing PHY domain precluding the for-
mation of bona fide Pfr. There may also be other prob-
lems in extrapolating from 1ZTU to...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
Ngày tải lên :
30/03/2014, 11:20
... conserved arginine-rich RNA binding
motif in cowpea chlorotic mottle virus capsid protein
results in virion structural alterations and RNA packa-
ging constraints. J Virol 79, 3277–3288.
37 Dertinger ... the initial value, but again we
observed an increase in light scattering, suggesting
Fig. 5. Urea and guanidine treatment of dlFG and sc-dlFG in the
presence of bis-ANS. (A) Effects of increasing ... link them. To facilitate
purification, both dlFG and a single-chain dimer,
sc-dlFG, contain an N-terminal six-histidine nickel-
affinity tag.
Using intrinsic fluorescence of Trp residues, extrinsic
fluorescence...
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