gfap and aquaporin 4 aqp 4 and preservation of neural pgc 1α in infarct brain after combined therapy with csa and epo

Báo cáo sinh học: "Combination of cyclosporine and erythropoietin improves brain infarct size and neurological function in rats after ischemic stroke" potx

Ngày tải lên : 18/06/2014, 22:20

... acid protein (GFAP) and aquaporin- 4 (AQP -4) in brain infarct area A) to E) Immunofluorescent staining (40 0 x) of glial fibrillary acid protein (GFAP) (white arrows) and aquaporin- 4 (AQP -4) (yellow ... release into the cytosol in the brain of group animals Further Reduction in Expressions of Glial Fibrillary Acid Protein (GFAP) and Aquaporin- 4 (AQP -4) and Preservation of Neural PGC- 1a in Infarct Brain ... coactivator-1a (PGC- 1a) in brain infarct area A) to E) Immunofluorescent staining (40 0 x) of PGC- 1a (yellow arrows) and neuron (white arrows) in brain infarct area (n = 6) Both numbers of PGC- 1a + cells and...

14
475
0

báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx

Ngày tải lên : 20/06/2014, 00:20

... http://www.occup-med.com/content /4/ 1/17 36 37 38 39 40 41 42 43 44 45 46 Hampton RY, Golenbock DT, Penman M, Krieger M, Raetz CR: Recognition and plasma clearance of endotoxin by scavenger receptors Nature 1991, 352: 342 - 344 ... Cluster n = 45 Irregular n = 86 Element Min Max Min Max Min Max C 26.5 68.6 17.2 60.0 14. 2 59.1 O 25.6 45 .0 25.7 44 .8 11.9 49 .4 S nd nd 0.8 4. 1 3.8 12.0 Fe 0 .4 1.9 0.3 12.0 0 .4 11.8 Cu 0 .4 1.0 0.7 ... size According to the mean comparison formula [15] with a standard deviation of 157.53 and a difference of 616, Za of 95% and a Zb of 80%, we obtained a sample size of In total, patients with mild...

11
511
0

báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx

Ngày tải lên : 12/08/2014, 03:20

... accumulation in papillae http://www.biomedcentral.com/ 147 1-2229/9/28 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 and hypersensitive response during the barley-powdery mildew interaction ... radicals and 1O2 [44 ] Finally, the presence of atrazine alone was found to induce the At3g55610 gene, which is involved in proline synthesis, with a higher intensity than under conditions of combina- ... effects of sucrose and atrazine This strongly suggested important interactions of sucrose and xenobiotic signalling or of sucrose and ROS signalling, thus resulting in induction of specific transcription...

18
265
0

Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot

Ngày tải lên : 16/03/2014, 18:20

... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248 –2 54 Prohaska, J.R & Ganther, H.E (1976) Selenium and glutathione peroxidase in developing rat brain J Neurochem ... one-half of that present in A 549 ) and poor GPx activity (Figs and 2); however, RD cells differ signiﬁcantly in GR activity and in particular in activity, gene expression and protein content of SOD ... cells is further conﬁrmed by indirect measurement of ROS obtained by comparing aconitase activity in standard conditions and after 18 h of incubation with PBN (Fig 7) In biological systems PBN [60,61],...

11
412
0

Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx

Ngày tải lên : 12/08/2014, 23:22

... damage in BLV-infected B cells These data are consistent with a virus-associated ROS scavenging mechanism conferring a reducing environment and interfering with apoptosis of the infected cell Finally, ... 15, 16, 17, 18, 19, 42 19, 5183 and 5193 were infected by a wild type proviral clone of BLV strain 344 (pBLV 344 ) [43 ] All infected animals were either in the asymptomatic stage of the disease (total ... 2'-7'-dichlorofluorescein upon ROS stimulation PBMCs from BLV-infected and control sheep were seeded in 24- wells plates at a density of 106cells/ml and incubated for 30 at 37°C with 10 μM of CMH2DCFDA After 24 h of...

10
328
0

Báo cáo y học: "Rheumatoid peripheral blood phagocytes are primed for activation but have impaired Fc-mediated generation of reactive oxygen specie" pptx

Ngày tải lên : 09/08/2014, 10:20

... 63 41 41 11 .4 309 9.8 9.6 3.3 - F 57 - 13.9 - - - - - Methotrexate, prednisolone, (depo-medrone) F 76 57 72 11 244 7.7 6.3 0.9 - F 49 - - 11 .4 2 04 7.2 4. 4 2.5 Azothioprine, meloxicam F 45 48 ... L-selectin, CD43, and CD 44 in synovial fluid neutrophils from patients with inflammatory joint diseases Evidence for a soluble form of L-selectin in synovial fluid Arthritis Rheum 19 94, 37: 342 - 348 Bond ... association of L-selectin expression on lymphocytes with any of the parameters measured, including ESR (data not shown) After stimulation with TNF-α, a rapid decrease in expression of L-selectin within...

11
587
0

báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps

Ngày tải lên : 11/08/2014, 11:22

... protein 262783_at At1g10850 -2 ,47 -0,37 -0,27 0,07 0 ,41 -0,21 -0,67 -0,10 ATP binding/protein serine/threonine kinase 261883_at At1g80870 -2 ,43 -0 ,48 -0,55 -0,36 -0,01 0, 84 0,85 0 ,44 protein kinase ... At5g47 240 5,78 1,17 2,21 0,35 1, 04 0,20 -0,11 -2,20 247 360_at At5g6 345 0 5,51 1,21 1 ,47 2, 84 1,08 0,19 -0,80 -0,81 266821_at At2g 448 40 5 ,40 1,03 2,60 2,75 1,79 0,28 0,36 -2, 74 2623 54_ at At1g 642 00 ... protein -1 ,48 263209_at AT1G10522 expressed protein -1 ,48 248 285_at AT5G52960 expressed protein -1,37 249 750_at 247 5 74_ at AT5G 245 70 AT5G61230 expressed protein ankyrin repeat family protein -1,35...

22
475
0

Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

Ngày tải lên : 05/03/2014, 23:20

... culture tube containing lL of FITC Annexin V and lL propidium iodide The cells were gently mixed and incubated for 15 at room temperature (25 °C) in the dark Finally, 40 0 lL of 1· binding buffer was ... the inability of MB and LPS to induce any impairment in mitochondrial function after h of treatment RCR and states 2, 3, and FCCP rates of MB- and LPStreated cells were the same as those of unstimulated ... results in signiﬁcant increase in UCP2 expression (3.6-fold, P < 0.05) On the other hand, analysis of the kinetics of induction of UCP2 protein in MDP-treated macrophages revealed a significant increase...

11
430
0

Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx

Ngày tải lên : 16/03/2014, 06:20

... translocation of cytosolic factors in 32 74 142 143 144 145 146 147 148 149 150 151 152 activation of the phagocyte NADPH oxidase: role of protein–protein interaction In Membrane Proteins: Structure, ... humans, and are involved in a variety of protein–protein interactions [167] The TPR domain of p67phox contains an insertion of 19 amino acids between the a-helices of TPR3 and TPR4: the insertion ... The binding requires Arg43 and Lys90 in human p47phox [ 144 – 146 ] (Fig 12) Intriguingly, in contrast to the complete conservation of Lys90, Arg43 is replaced by Lys in the mouse and by Thr in the...

29
618
0

Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps

Ngày tải lên : 09/08/2014, 08:23

... penicillin G, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B After reaching 80% confluence, the cells were rinsed twice with HANK's solution and placed in serum-free medium, containing 0.05% ... PMA, p ≤ 0.05), and 2 .4- fold and 3.7-fold after treatment with SIN-1 at concentrations of 10 and 20 μM, respectively (control versus SIN-1, p ≤ 0.05) Increasing nitric oxide content of the medium ... compared with the monolayer cultures, with 1.7-fold, 2.5-fold and 3.6-fold increases in NO content reported after treatment with 1, 10 and 20 μM of SIN1, respectively (control versus SIN-1, p...

8
332
0

Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species

Ngày tải lên : 24/08/2014, 12:59

... loaded, and served as internal controls Calcein was injected days after the first loading bout, and alizarin was injected days after the first loading bout These injections were administered interperitoneally ... hours in a saline bath, and mechanical testing was conducted by threepoint bending using a microforce testing machine (Vitrodyne V1000; Liveco, Inc., Burlington, VT) Loads were applied in the ... Calcein was injected days after the first loading bout, and alizarin was injected days after the 17 first loading bout These injections were administered interperitoneally Both the right and left...

73
255
0

Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

Ngày tải lên : 25/10/2012, 11:18

... replacement [32,35] In this study, 616 mutations were identified in the D-Loop region of mtDNA, including 48 9 point mutations, 81 insertions and 46 deletions, and 89 .4% of point mutations (43 7 /48 9) were ... μg/ml, 240 μg/ml, 48 0 μg/ml or 960 μg/ml Hp extract for 24 h and those in the other group maintained in the medium containing 48 0 μg/ml Hp extract and μmol/L of DHR-123 for h, h, h, 12 h and 24 h ... region including 48 9 point mutations, 81 insertions and 46 deletions In addition, 89 .4% of the point mutations (43 7 /48 9) were G → A; C→T or A → G; T→C transition Except in cells treated with 60...

12
557
2

Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc

Ngày tải lên : 23/03/2014, 09:21

... Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons Invest Ophthalmol Vis Sci 47 , 1016–1023 Stein I, Itin A, Einat P, Skaliter R, Grossman Z & Keshet E (1998) Translation of vascular ... why the retina is capable of tolerating an almost 10 times longer period of ischemia than brain They described how the presence of hypoxia in the retina is consistent with the ﬁnding of increased ... 50 40 Neuroglobin as ROS scavenger? N * H48 48 R8 30 48 R 24 20 * * * 10 eyes brain muscle heart liver Fig H2O2 level measured in mice under hypoxic conditions and after reoxygenation Evolution of...

6
391
0

Báo cáo khoa học: Is there more to aging than mitochondrial DNA and reactive oxygen species? pot

Ngày tải lên : 29/03/2014, 22:21

... Effects of Ni(II) and Cu(II) on DNA interaction with the N-terminal sequence of human protamine P2: enhancement of binding and mediation of oxidative DNA strand scission and base damage Carcinogenesis ... Walker WF, Hernandez-Rosa E, Trevisson E, Delplace S, Desnuelle C, Shanske S, Hirano M et al (2008) A Functionally 57 84 139 140 141 142 143 144 145 146 147 148 149 150 151 Dominant Mitochondrial ... mitochondria of transgenic animals [1 64] Two founder lines were established, 40 33 and 44 03 The expression of the transgene was mosaic, with hearts showing the highest level of expression, but with only...

20
523
0

Báo cáo Y học: Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species pot

Ngày tải lên : 31/03/2014, 21:21

... 2.8 1 .4 ND in NADH:quinone oxidoreductase during the purification of the Na+-NQR according to [6] In parallel with a decrease in signal intensity of the Fe–S cluster, there was a decrease in the ... fragment of the FrdA subunit [19] The stoichiometry and localization of the flavin cofactors of the Na+-NQR are a matter of debate [7,20] A contamination of the Na+-NQR with FrdA that contains a ... addition of NADH in the presence of thenoyltrifluoroacetone (TTFA), a specific inhibitor of succinate dehydrogenase and fumarate reductase, prevented the increase in signal intensity in the g %...

6
307
0

báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf

Ngày tải lên : 10/08/2014, 10:20

... as indicated in (A), and then stained with annexin V and PI followed by flow cytometry analysis Early apoptosis is defined by Annexin V+/PIstaining (Q4) and late apoptosis is defined by Annexin ... staining (Q2) (C) and (D) HeLa cells were treated with cisplatin (8 μM) or saikosaponin-a (10 μM) or saikosaponin-d (2 μM) individually or combination of saikosaponin and cisplatin for 24 h and ... with NAC (1 mM) for 30 or remained untreated and then treated with saikosaponin-a (10 μM) or saikosaponin-d (2 μM) or cisplatin individually or combination of saikosaponin and cisplatin for 48 ...

8
395
0

Báo cáo y học: " Early The tripeptide feG regulates the production of intracellular reactive oxygen species by neutrophils" pps

Ngày tải lên : 11/08/2014, 08:21

... CD11b/c and CD49d The effect of antigen challenge on the expression of CD11b/c β 2integrin and CD49d β 1-integrin on circulating neutrophils Integrin expression on the cell surface of neutrophils ... eotaxin and eosinophils in eosinophilic gastrointestinal inflammation Nat Immunol 2001, 2 (4) :353-360 Teixeira MM, Das AM, Miotla JM, Perretti M, Hellewell PG: The role of lipocortin-1 in the inhibitory ... ovalbumin (OVA) sensitization http://www.journal-inflammation.com/content/3/1/9 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 elicits IgE adjuvant activity but attenuates OVA-induced...

9
214
0

Báo cáo khoa học: "A Reactive oxygen species: toxic molecules or spark of life" docx

Ngày tải lên : 12/08/2014, 23:21

... 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury Proc ... increase in ROS in sepsis is of pathophysiological importance Oxygen radical scavengers reduce lung injury in animal models [43 48] and improve hemodynamics [48 ,49 ] An interesting and potentially clinically ... product of OONO– and tyrosine is 3-nitrotyrosine and antibodies against it are used as a ‘footprint’ of protein oxidation [40 ] Oxidation of proteins also can lead to products with carbonyl groups [41 ]...

8
226
0

Báo cáo y học: "Endogenous TGF-β activation by reactive oxygen species is key to Foxp3 induction in TCR-stimulated and HIV-1-infected human CD4+CD25- T cells" docx

Ngày tải lên : 13/08/2014, 05:22

... the indicated time points and then intracellular ROS was stained with DHE The mean fluorescence intensity (MFI) of DHE in a single cell was measured with FACS A aliquot of cells was stained with ... blot analysis of Foxp3 protein in cultured CD4+CD25- T cells with indicated reagents (72 hrs) B FACS analysis of intracellular Foxp3 protein cultured with TCR and CD28 in the presence of antiTGF-β1,2,3 ... underlying TCRinduction of Foxp3 in human T cells is not understood Since TGF-β has been implicated in the induction of Tregs in murine cells, we set out to investigate whether TGF-β has a role in...

16
250
0

novel insights into mechanism of antioxidant-mediated cellular protection through the quenching of intracellular reactive oxygen species

Ngày tải lên : 14/11/2014, 06:34

168
137
0