functional analysis of the cervicalfunctional analysis of intergenic
Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
Ngày tải lên :
18/02/2014, 16:20
... eggs in the
one-cell stage to visualize the localization and to mea-
sure the mobility of the components in the PGCs. The
medaka embryo was peeled off the chorion, and the
segment containing the ... on the same setup of
the laser scanning microscope as used for FCS analysis.
The detection gain was adjusted to the fluorescence of the
GFP fusion proteins almost at the saturation level of the
detector, ... in the HeLa cell [24], suggesting
the existence of some interactive cellular partner.
FRAP analysis of the nuage revealed the slow recovery
of Olvas–GFP (Fig. 3B). When the whole part of the
single...
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Situational analysis of the socioeconomic conditions of orphans and vulnerable children in seven districts in Botswana pot
Ngày tải lên :
06/03/2014, 01:22
... Morupule. The local mine
Morupule Colliery supplies the coal for the power station. Most of the people living in
Palapye are employed by either the power station or the colliery. The other groups of ... coping with the burden of orphans and providing support. The caregivers
acknowledge that there are lots of OVC who are cared for by the government. As in
Palapye, one of the strengths of the government ... take care of their own needs as well as the needs
of the children with whom they live. The conditions under which OVC are raised indicate
that most of them live in poverty. Indicators of poverty...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
Ngày tải lên :
16/03/2014, 03:20
... envelope of the bound protein. The
percentage of protein bound to RNA was the ratio between
the value of bound protein and the total protein present in
the sample. The relative percentage of binding ... evaluation of the available model
on the interaction and cleavage of the RNA substrate
and the Kid toxin is of interest in itself because it is
the basis of important cellular roles of this toxin ... test the formation of a
stable heterooctameric Kid
2
–Kis
2
–Kid
2
–Kis
2
complex
on the parD promoter [9]. We further analysed the
effects of the mutations on the co-regulatory activity
of the...
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analysis of the final scenes of alfred hitchcock
Ngày tải lên :
21/03/2014, 21:56
... shifting
points of view, and
light and dark to help explain the relationships between Alicia,
Devlin,
Sebastian and Mrs. Sebastian, and an overall theme of being
trapped. An
analysis of the film from the ... scene to the final
scene in the
film shows how the above tools lead to a better understanding of
the
character's motivations.
The most obvious recurring object in the final scenes is
the poisoned
coffee ... pours the
coffee into the cup for Alicia, and sets it on a small table in
front of her.
Here, Hitchcock not only zooms in on the small teacup, but
heightens the sound
it makes connecting to the...
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Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot
Ngày tải lên :
23/03/2014, 13:20
... no. Q7QKF0) where the Asp235 is
missing. In this latter case, the absence of the Asp235
can be explained in the light of the model of the inter-
action of the substrate with the active site (vide ... such enzymes. The
structural and functional roles of the residues involved in the substrate
selection and binding are proposed through the analysis of the predicted
interactions of the model active ... moiety tethered to the phosphopantetheinyl
group and the free amino group of the downstream
aminoacyl moiety, thus facilitating the translocation
of the growing chain onto the next module. The
structural...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
Ngày tải lên :
23/03/2014, 15:20
... Likewise, these glycopro-
teins are localized in lysosome, however, Man6-SO
4
accounts for the majority of the sulfated sugar.
In conclusion, the results obtained provide evidence
of the nature of the ... that of the
untreated sample (Fig. 5A), assuring the high-mannose
nature of the sulfated glycans.
Table 1. m ⁄ z-Value, composition and structure of the high mannose and complex type glycans of ... UV-MALDI-TOF MS analysis of the
oligosaccharides released from C-terminal
domain by PNGase F treatment in the linear
negative-ion mode using nor-harmane as
matrix. (A) Analysis of the total oligosaccha-
ride...
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Fundamentals of Current Pension Funding and Accounting For Private Sector Pension Plans: An Analysis by the Pension Committee of the American Academy of Actuaries pptx
Ngày tải lên :
29/03/2014, 14:20
... associated with the passage of time
during the year. This is offset against the other cost items and is generally the expected long-
term rate of return on assets multiplied by the beginning of year ... percent of the greater of the plan’s PBO or MRVA.
The total of the above pieces of the NPPC can produce a negative expense, which appears as income on
a company’s financial statements.
The accrued ... that the retirement benefit is based on the pay
at retirement. To allow the plan sponsor to recognize the cost of the plan gradually over the
participant’s lifetime, the actuary considers the...
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Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot
Ngày tải lên :
29/03/2014, 22:21
... data
not shown). On the basis of these results, we conclude
that the absence of either of these two clusters of basic
amino acid within the linker does not affect the ability
of the protein to preferentially ... tyrosine on the
scissile phosphate in the DNA, resulting in the cova-
lent attachment of the enzyme to the 3¢ end of the
broken strand [2]. Rotation of the duplex region
downstream of the break ... manifestation of the preference
for supercoils. It is noteworthy that the elimination of
either of the clusters of basic amino acids within the
linker region (Fig. 1) does not affect the preference of
the...
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Báo cáo khoa học: Structural adaptation to low temperatures ) analysis of the subunit interface of oligomeric psychrophilic enzymes pdf
Ngày tải lên :
30/03/2014, 03:20
... interpreted in the light
of the work of Bahadur et al. [36]. These authors stud-
ied the subunit interfaces of 122 homodimers, and
showed that the distribution of the area between the
rim and core ... from those of their
mesophilic and thermophilic homologs. The most vari-
able features are the increase in the number of ionic
interactions, the decrease in the number of hydrogen
bonds, the decrease ... using a set of 512 points located
on a sphere around each atom. The sphere interaction radius
of each atom is equal to the sum of the van der Waals radius
of the atom type plus the radius of a water...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Ngày tải lên :
21/02/2014, 15:20
... and then the energy
minimization of the model was iterated 300 cycles using the
conjugate gradient methods with the program
DISCOVER
_3
in
INSIGHT II
. To construct the dimeric form of LPL, the
model ... as the high-
affinity peak of wild-type LPL. The LPL in the eluate was
detected by Western blot with LPL polyclonal antibody
7640. The fraction number of the high-affinity peak was the
same for the ... mutant, the
two LPL peaks both eluted at lower salt concentrations
than those of wild-type LPL. The R405A mutant exhibited
an earlier elution of the high-affinity peak, whereas the
elution of the...
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Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt
Ngày tải lên :
15/03/2014, 10:20
... eliminate the possibility of the functional roles of
the )643T>C and )1219G>A SNPs as part of the
extended APOH promoter transcriptional machinery.
To further substantiate the functional ... Further studies will be needed to explore the
mechanism for these associations.
APOH promoter SNPs explain a small proportion
of the variance in APOH expression. Therefore, the
ability of these ... with the univariate data,
none of the haplotypes defined by the minor alleles at
the APOH promoter SNPs reached significance.
Although the )32C>A SNP was significant in the
univariate analysis, the...
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Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
Ngày tải lên :
16/03/2014, 13:20
... with the A
8
B
8
isoform of GAPDH lead-
ing to the A
2
B
2
isoform with a higher NADPH-
dependent activity [39].
By analogy with the CTE of the isoform of GAPDH
[15,37], we hypothesize that the ... Sparla et al.on
the A
2
B
2
isoform of GAPDH [24,37], formation of a
disulfide bridge in the oxidized CTE prevents the inter-
action between the 2¢-phosphate of NADPH and the
side chain of Arg77 (spinach ... We
therefore mutated Asp36 and Glu39 of CP12 into Ala
or Lys. To test whether the interactions of these
mutants with the other partner, GAPDH were im-
paired, we tried to reconstitute in vitro the...
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- one of the ultimate goals of teaching
- and increased knowledge of the human understanding of how the operation of the physical world surroundings through controlled methods
- sixth conference of the european chapter of the association
- the turn of the screw analysis
- publications of the astronomical society of the pacific abbreviation
- the turn of the screw analysis miles
- the turn of the screw analysis of the governess
- the turn of the screw analysis literary devices
- the turn of the screw analysis essay
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