from in silico data to the laboratory experiment

Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot

Ngày tải lên : 23/03/2014, 13:20

... cofactor binding site for either pyrroloquinoline quinone (PQQ) or NADPH In particular, AASDH, containing a PQQ binding domain, is supposed to be involved in lysine degradation and to convert the ... domain of the ACV synthetase from Penicillium chrysogenum [7] The results of the docking experiments indicated (Fig 4) that the possible binding modes cluster into two solutions According to the ﬁrst ... site of the phosphopantetheine attachment The resulting alignment was ﬁnally utilized to train the HMM The resulting HMM was used to align a subset of the A-T domains listed in Table The alignment...

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Báo cáo khoa học: The mitochondrial permeability transition from in vitro artifact to disease target ppt

Ngày tải lên : 30/03/2014, 11:20

... step in steroid synthesis [159] The PBR also binds some porphyrins, including protoporphyrin IX, a potent inducer of the PTP [160], and is thought to be involved in transport of porphyrins into the ... devoted to identifying the signaling pathways linking myocardial protection to PTP inhibition, which may involve protein kinase Ce [217] and mitogen-activated protein kinase kinase [218] An integrated ... of interest in the PT as an effector mechanism of cell death, however, only followed the demonstration that in the course of apoptosis, cytochrome c is released into the cytosol [72], together...

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Báo cáo y học: " An integrated approach to diagnosis and management of severe haemoptysis in patients admitted to the intensive care unit: a case series from a referral centre" ppsx

Ngày tải lên : 12/08/2014, 15:20

... for the therapeutic management of haemoptysis in this clinical setting To summarize, a multidisciplinary approach remains the cornerstone for the management of severe haemoptysis Bedside clinical ... contributions 18 Dr Fartoukh had full access to the data and takes responsibility for the integrity of the data and the accuracy of the data analysis 19 20 Study concept and design: Fartoukh, Cadranel ... was then performed, using catheters ranging from to 6.5 French Embolisation was performed when the bronchial arteries appeared to be the source of haemoptysis (tortuous hypertrophy, systemic -to- pulmonary...

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Báo cáo y học: " In silico modeling indicates the development of HIV-1 resistance to multiple shRNA gene therapy differs to standard antiretroviral therapy" docx

Ngày tải lên : 13/08/2014, 01:20

... if the shRNA were allowed to inhibit infection and act as a Class gene therapy, then the viral productivity of each infected neighbour was modified by the inhibition by the shRNA in the uninfected ... reduction in efficacy of each shRNA from 80% to 60% and kept all other parameters unchanged The reduction in efficacy from 80% (S1) to 60% (S4) led to a slight increase in the number of infected ... HSC from 20% to an apparently more biologically relevant 1% A reduction in the proportion of HSC containing shRNAs from 20% (S1) to 1% (S5) increased the number of infected cells from 35 to 42%...

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Overview of Plant Biotechnology from Its Early Roots to the Present

Ngày tải lên : 25/10/2013, 05:20

... biotechnologists to develop new crops involving the tools of gene and metabolic engineering For example, scientists have been working on tomatoes that can be vine-ripened and shipped without bruising Others ... biotechnology Using established cell cultures, it is now possible to deﬁne the rate-limiting step in biosynthesis by determining accumulation of presumed intermediates, characterizing the limiting enzyme ... reduce the need for costly and highly toxic pesticides This applies also to nutritionally important constituents in crops The new techniques from the gene and metabolic engineering toolbox will bring...

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Tài liệu Actions Against Abuse of the Global Financial System: Report from G7 Finance Ministers to the Heads of State and Government docx

Ngày tải lên : 17/02/2014, 21:20

... simply in order to gain access to the financial system If there is obscurity about their ownership, banks and other financial institutions may not be able to discover the identity of the beneficiary ... support for the continuation of this work b International Payments System: We urge the financial community to find ways to identify originators in executing cross-border payment orders In this respect, ... Moving forward from the Birmingham and Cologne Summits, we value the opportunity to discuss a range of measures to fight money laundering We agree to continue to strengthen our efforts within...

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**Tài liệu Higher Education in ‘Business Administration’ in Spain: Adapting to the European Area of Higher Education * pptx**

Ngày tải lên : 18/02/2014, 11:20

... in «Tourism» originates from the existing university three-year degree (Diploma) «Diplomado in Tourism» that exists since the sixties in the professional sector and that was incorporated to the ... Education in ‘Business Administration’ in Spain HIGHER EDUCATION IN SPAIN WITHIN THE FRAMEWORK OF THE «EUROPEAN AREA OF HIGHER EDUCATION» As it is well known, the starting point of the process to achieve ... Education in ‘Business Administration’ in Spain NOTAS TÉCNICAS 595 several others: a degree in «Tourism», a degree in «Accounting and Finance», and yet another in «Human Resources Management» The degree...

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Tài liệu Báo cáo khoa học: Fermentative lifestyle in yeasts belonging to the Saccharomyces complex ppt

Ngày tải lên : 19/02/2014, 02:20

... generation, due to amino acid biosynthesis Much of the generation of NADH during amino acid biosynthesis takes place in the mitochondria Because of the block in the respiratory chain caused by the addition ... translocate ATP produced in the cytoplasm into mitochondria, and the ability to adjust the redox balance, play a very important role in independence from oxygen [9–14] In S cerevisiae, three genes ... antimycin A, NADH should then be reoxidized through shuttle mechanisms with the cytoplasm [14] Acetoin acts as a redox sink at the cytoplasmic level, being reduced to 2,3-butanediol by the cytosolic...

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Tài liệu Báo cáo khoa học: "Contrastive accent in a data-to-speech system" doc

Ngày tải lên : 22/02/2014, 03:20

... depend on the linguistic expressions which are chosen to express the input data; the data can be expressed in an indirect way, as in (1)a, without influencing the prediction of contrast The approach ... instance, the contrast in (1) is based on the knowledge that kicking the ball into the wrong goal implies scoring a goal for the opposing team In a HOU approach, the contrast in this example might ... express semantically parallel information allows us to make use of the world (or domain) knowledge incorporated in the design of these data structures, without having to separately encode this knowledge...

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Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot

Ngày tải lên : 07/03/2014, 21:20

... than the SsAH-WT (Table 1) The behaviour of this mutant can be rationally explained on the basis of the homology model, as the increased occupancy deriving from the arginine side chain in position ... obtained by combining, weighting and screening the results of several multiple alignment programs The 3D model of the SsAH-K96R mutant was obtained from the 3D model of SsAH-WT by replacing the ... useful to suggest functional hypothesis especially when they are based on a convincing evolutionary relationship, as in this case Nevertheless their conclusions need to be experimentally veriﬁed In...

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Báo cáo khoa học: Utilizing logical relationships in genomic data to decipher cellular processes pptx

Ngày tải lên : 07/03/2014, 21:20

... to the uncertainty coefﬁcient (referred to hereafter as an information coefﬁcient) comparing either the logically combined vectors or individual vectors A or B with vector C, conditioned on the ... relationships combining two binary states to match a third state We systematically examined phylogenetic data, in the form of binary presence ⁄ absence vectors, in an attempt to identify the logic relationships ... appear to affect various inhibitory roles within the cell For instance, thyrotro5115 Utilizing logical relationships in genomic data pin-releasing hormone degradation enzyme (TRHDE), protein tyrosine...

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History Of Modern Philosophy From Nicolas of Cusa to the Present Time pot

Ngày tải lên : 16/03/2014, 03:20

... which constitutes the perfection of the whole and of the parts, that the higher is in the lower, the cause in the effect, the genus in the individual, the soul in the body, reason in the senses, and ... desired to see the unity in the divine government of the world embodied in a pyramid of states with the papacy at the apex: above the individual states was to come the province, then the kingdom, the ... Everything degenerates, science itself only contributes to the fall therefore, back to the happy beginnings of things! If, finally, we inquire into the position of the Church in regard to the questions...

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Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx

Ngày tải lên : 23/03/2014, 07:20

... present in the C-terminus of the preprotein [11] Therefore, we constructed a pTic22DC mutant in which the carboxy-terminal amino acids were deleted but still contained the N-terminal targeting signal ... envelope Therefore, we repeated the cross-linking experiments in the presence of an excess of the soluble chloroplast preprotein pOE33 to compete for speciﬁc binding, whereas the control experiment ... MGD1) in the same vectors, using standard PCR protocols Tic22DC was produced by removal of the coding sequence corresponding to 225 C-terminal amini acids of the preprotein Transcription of linearized...

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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

Ngày tải lên : 30/03/2014, 20:20

... reported to change from 1.6 h in early G1 to 12 h in late G1 and h in S and G2 ⁄ M phase [12] Similarly, cyclin A and B1 mRNA half-lives increased in RKO cells from about 2.5 h in G1 to 18 h in S ... inhibition to explain the decay of an mRNA that ﬂuctuates 10-fold in the cycle At present it is unclear what triggers the decrease in the level of the 2.5-kb cyclin B1 mRNA at the M ⁄ G1 transition Mitotic ... amphidicolin block, the half-life of cyclin B1 mRNA measured after actinomycin D addition increased from 1.2 h in early G1 to 12 h in G2 ⁄ M phase [11] With the same method the cyclin A mRNA half-life...

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straumann n. from primordial quantum fluctuations to the anisotropies of cmbr

Ngày tải lên : 24/04/2014, 17:08

... with the Advanced Camera for Surveys (ACS) aboard the Hubble Space Telescope (HST) The quality of the data and some of the main results of the analysis are shown in Fig The data points in the top ... properties Using these correlations it became possible to reduce the remaining intrinsic dispersion, at least in the average, to ≃ 0.15mag (For the various methods in use, and how they compare, ... received at the time to + to , we obtain from the last equation to + to te +∆te dt = a(t) to te dt a(t) (27) For a small ∆te this gives to ∆te = a (to ) a(te ) The observed and the emitted...

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measuring efficiency and explaining failures in banking application to the russian banking sector

Ngày tải lên : 03/06/2014, 02:06

... of financing to mining Central (excluding Moscow), North West and Volga regions prevail in issuing credit to manufacturing, since these regions host most of the large industrial enterprises The ... 2005).26 The increase in the banking sector’s rating in 2005 was due to the growth in intermediation, establishment of more stringent capital adequacy requirement and obligatory use of International ... of the other approaches used to model deposits in the banking sector In Chapter we investigate bank failures The financial crisis of 1998 and turbulence on the market in summer 2004 raise the...

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from marx and mao to the market the economics and politics of agricultural transition mar 2006

Ngày tải lên : 10/06/2014, 23:16

... OB to OF and price reform increases it further from OF to OD (or ﬁrst from OB to OC and then from OC to OD if the sequencing of the reforms is the other way around) Third, to what extent are these ... the farm’s point of view (and in the terminology of production economics), the higher output can be said to come from the rise in the farm’s ﬁxed factor From the point of view of the relationship ... depending on the initial conditions In Country 1 output goes up: from qo (point A) to qpϩr (point D) In Country the positive output effects from the property rights reform from qp (point C) to qpϩr...

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Báo cáo y học: " An RNAi in silico approach to find an optimal shRNA cocktail against HIV-1" potx

Ngày tải lên : 11/08/2014, 21:21

... describe in detail their in silico analyses; the features for silencing activity they evaluated, the filters or threshold they used, whether they included a free energy cut-off, their approach to ambiguities ... combinations Another reason could lie in the three nearby amino acids (either to the left or to the right of the motif), which can be encoded by more than two codons due to the redundancy of the ... percentages in order to be able to compare results between different MSAs The number of viral sequences included in the analyses (NSI) and the number of viral variants (VV) -the latter including dominant...

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Báo cáo khoa học: "Sales of oseltamivir in Norway prior to the emergence of oseltamivir resistant influenza A(H1N1) viruses in 2007–08" pps

Ngày tải lên : 12/08/2014, 04:21

... private stockpiling of oseltamivir was causing empty pharmacies in the beginning of the year In the US, increased media attention on the pandemic flu also caused private stockpiling of the drug ... Competing interests The authors declare that they have no competing interests Authors' contributions SHH participated in the initiation of the data collection, analysis and drafting of the manuscript; ... analysed data from the Wholesales Register and NorPD and participated in drafting of the manuscript; KB, OH and SD all participated in the drafting of the manuscript and PA participated in the initiation...

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Báo cáo khoa học: " Sales of oseltamivir in Norway prior to the emergence of oseltamivir resistant influenza A(H1N1) viruses in 2007–08" pot

Ngày tải lên : 12/08/2014, 04:21

... private stockpiling of oseltamivir was causing empty pharmacies in the beginning of the year In the US, increased media attention on the pandemic flu also caused private stockpiling of the drug ... Competing interests The authors declare that they have no competing interests Authors' contributions SHH participated in the initiation of the data collection, analysis and drafting of the manuscript; ... analysed data from the Wholesales Register and NorPD and participated in drafting of the manuscript; KB, OH and SD all participated in the drafting of the manuscript and PA participated in the initiation...

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