expression collagen and a1r erk1 2 activity in the in vitro organ culture model of early coronary atherosclerosis

Báo cáo y học: "roteoglycan 4 downregulation in a sheep meniscectomy model of early osteoarthritis" docx

Ngày tải lên : 09/08/2014, 07:20

... turnover of PRG4 during the early stages of the disease process The aim of the present study was therefore to determine the changes in cartilage PRG4 expression and immunolocalisation occurring early ... of PRG4 mRNA levels in the superficial zone of normal articular cartilage Decreases in the presence and/ or viability of the superficial zone cells occurring early in the present model of OA therefore ... ovine model [ 12] The modified Mankin score has a range of to 29 , the value increasing with severity of cartilage degeneration In each compartment the worst score evident for the region examined...

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Báo cáo hóa học: " Hantaviruses and TNF-alpha act synergistically to induce ERK1/2 inactivation in Vero E6 cells" docx

Ngày tải lên : 20/06/2014, 01:20

... in synergy with hantaviruses to inhibit ERK1/ 2 activity The additional effects of TNF-α on ERK1/ 2 inhibition were from 2- to 20 fold (Figure 4) Interestingly, TNF-α could inhibit ERK1/ 2 also in ... phosphorylated ERK1/ 2 (p -ERK1/ 2) or total ERK1/ 2 (A) Bands were subjected to intensity analysis (ImageJ software; http://rsb.info.nih.gov/ij) and the amount of p -ERK1/ 2 related to mock sample at 14 and 25 ... proteins have other essential roles in the kidneys They promote tubular epithelial cell proliferation [27 ,28 ] and epithelial cell barrier resistance [29 ,30] thereby maintaining the integrity of a functional...

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Báo cáo khoa học: Aegyptin displays high-afﬁnity for the von Willebrand factor binding site (RGQOGVMGF) in collagen and inhibits carotid thrombus formation in vivo ppt

Ngày tải lên : 22/03/2014, 21:20

... 2A), indicating that the G primary sequence is indeed sufficient for the interaction Aegyptin binds with low affinity to GPVI and integrin a2b1 binding sites in collagen Sequences involved in collagen ... for collagen (lower right panel) Aegyptin binds with high affinity to the vWF binding site in collagen, independently of hydroxyproline In an attempt to identify the binding sites involved in collagen ... or collagen and aegyptin displayed biphasic binding kinetics, with relatively similar ka1 and ka2 rates On the other hand, the off-rates, kd1, for the (GPO)10 and GFOGER interactions with the inhibitor...

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báo cáo hóa học: " Vascular endothelial growth factor-A (VEGF-A) and chemokine ligand-2 (CCL2) in amyotrophic lateral sclerosis (ALS) patients" ppt

Ngày tải lên : 19/06/2014, 22:20

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Báo cáo hóa học: " Silver and Gold Nanoparticles Alter Cathepsin Activity In vitro" potx

Ngày tải lên : 21/06/2014, 08:20

... to the cell itself The inhibitory effects of the NPs on cathepsin activity were observed within as little as h of incubation (data not shown); following 24 h of incubation with the NPs, the cathepsin ... direct interaction of the Ag-NPs with the proteins; however, the internalized Ag-NPs are likely to interact with many proteins in the cell and not just the cathepsins, therefore diluting the dose ... natural function of the cells in which they are incorporated Both Ag- and Au-NPs are capable of recognizing and readily binding thiol groups of proteins and other ligands, suggesting that they would...

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Báo cáo y học: "Markers of B-lymphocyte activation are elevated in patients with early rheumatoid arthritis and correlated with disease activity in the ESPOIR cohort" pdf

Ngày tải lên : 09/08/2014, 14:22

... reported in RA, with Th2 (interleukin [IL]-4, IL-13) and IL-17 expression increasing temporarily in the synovial fluid of patients with very early RA [29 ] Th2 profiles favor B-cell activation and ... 2. 9 (2. 1–3.7) 2. 5 (1.9–3.3)

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Báo cáo y học: "Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro" pptx

Ngày tải lên : 09/08/2014, 14:22

... important intellectual content BK was involved in the conception and design of the study, analysis and interpretation of the data, and did most of the drafting of the manuscript All authors read and ... 12 Competing interests The authors declare that they have no competing interests 13 Authors' contributions HV made the acquisition of data and part of the analysis of the data, and was also involved ... degradation [21 ] In order to investigate the influence of TNFα on the meniscus we present a bovine in vitro model that allows the isolation of meniscal tissue explants of defined geometry and anatomical...

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báo cáo khoa học: "Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro" potx

Ngày tải lên : 11/08/2014, 00:22

... selectively increased local drug dose in experimental colitis in rats J Pharmacol Exp Ther 20 05, 315:196 -20 2 22 23 24 25 26 27 28 29 30 31 32 Lee KD, Hong K, Papahadjopoulos D: Recognition of liposomes ... (Figure 7C) revealed the typical profile of aggregated drug The spectrum in Figure 7C indicates a certain amount of monomeric drug not present in the other spectra (Figure 7A and 7B) Possibly, CMC ... BF/AmB was better from the point of view of reduced nephrotoxicity [22 ] Furthermore, cationic surfactants and polymers have an effect on integrity of red blood cells [28 ] Therefore, similar studies...

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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf

Ngày tải lên : 12/08/2014, 23:21

... trial of nevirapine plus zidovudine versus Page 14 of 16 (page number not for citation purposes) Retrovirology 20 09, 6:44 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 zidovudine alone in p24 ... was the only method employed In the absence of the second drug, the reciprocal of the ratio of the initial rate in the absence of inhibitors over v (v0/v) is plotted against the concentration of ... [21 ] Interest in the NRTI+NRTI combinations was first ignited during the HIV monotherapy era by the surprisingly synergistic effects of AZT+ddI both in vitro and in clinical trial studies [22 -24 ],...

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Báo cáo y học: "Expression and reactivation of HIV in a chemokine induced model of HIV latency in primary resting CD4+ T cell" potx

Ngày tải lên : 13/08/2014, 01:21

... established in resting memory CD4+ T-cells in vitro following incubation with the chemokines CCL19 and CCL21 (ligands for CCR7), CXCL9 and CXCL10 (ligands for CXCR3) and CCL20 (ligand for CCR6) [11, 12] ... established an in vitro model of HIV latency following incubation of resting CD4+ T-cells with the CCR7 ligands, CCL19 [11, 12] We have shown here that, in this in vitro model of HIV latency, there was ... combination of prostratin and the HDACi vorinostat in both a cell line and primary cell model of latent HIV infection [46] Herein we also demonstrated the additive effects in activation of HIV...

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Tài liệu The Early Detection Research Network: Investing in Translational Research on Biomarkers of Early Cancer and Cancer Risk docx

Ngày tải lên : 14/02/2014, 21:20

... forms in the tissues of the colon (the longest part of the large intestine) Most colon cancers are adenocarcinomas (cancers that begin in cells that make and release mucus and other fluids) Definition ... validation; one of the tests included a panel of markers consisting of MIF-1, prolactin, osteopontin, IGF -2, leptin, HE-4 and others Studies are underway targeting pre-cancers of the cervix to ... University of Maryland School of Medicine Overview 17 Early Challenges Establishing and sustaining collaborations while ensuring a smooth flow of discoveries from the laboratory to the clinic were clearly...

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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

Ngày tải lên : 18/02/2014, 04:20

... trafficking These activating steps include furin-mediated cleavage at the Arg279Glu280 peptide bond [13], reduction of the disulfide bond linking Cys265 and Cys287 [14], and removal of the C-terminal ... that of the cysteine protease cathepsin B and the aspartic acid protease cathepsin D, as indicated by the following observations: (a) the inhibitor profile of the endosomal ETA-degrading activity ... determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro transfer of the ADP-ribosyl moiety of NAD+ to EF -2 by ETA, but not in the release...

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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

Ngày tải lên : 19/02/2014, 18:20

... Balish MF (20 04) Cellular engineering in a minimal microbe: structure and assembly of the term- FEBS Journal 27 2 (20 05) 28 92 29 00 ª 20 05 FEBS 17 18 19 20 21 22 23 24 25 26 inal organelle of Mycoplasma ... N-terminus instead of a free amino group [ 32] Therefore, the asparagine in position 26 was labeled within the intact protein at its a-amino group and represents the amino terminal amino acid of ... represents the amino terminus of the protein Using this method, we showed that asparagine 26 represents the amino terminus of P40 Knowing, that the ﬁrst amino acids of the processed P40 and P90 is the...

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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Ngày tải lên : 16/03/2014, 12:20

... affects the properties of a variety of proteins with common biochemical activities, such as the binding to single-stranded nucleic acids and the remodelling of their secondary structures A model involving ... remodelling activity and, consequently, might affect the translational control In the other reported model for CNBP function, CNBP was found binding to the purine-rich strand of the CT element from the ... Modulation of the concentrations and ⁄ or the biochemical activities of either CNBP or hnRNP K could in uence the ability of the other factors to bind to the CT element and, thus, modulate CT element activity...

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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

Ngày tải lên : 16/03/2014, 23:20

... oligomerization [22 25 ] Since a functioning zinc-binding domain may be present in the Ab sequence of C99, we hypothesized that zinc may affect the oligomerization state of C101-3FLAG Therefore, the molecular ... within the literature concerning the molecular mass of c-secretase complexes and activity Since our assays measure activity without the need of overexpressing the c-secretase complex components and ... 5C), conﬁrming that column chromatography in the presence of 0.5% CHAPSO resulted in a loss of c-secretase activity Interestingly, when Nct, PS1, and PS2 immunoreactivity was tested in these 0.5%...

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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf

Ngày tải lên : 30/03/2014, 08:20

... cells expressing the recombinant mPC1 ⁄ 3, the presence of the 87 kDa form in excess of the 66 ⁄ 71 kDa facilitates isolation of the enzyme and helps in maintaining the enzymatic activity at a ... cooperate in the activation of an enzyme In the case of proPC1 ⁄ 3, removal of the various structural and functional domains is a sequential and coordinated event culminating in removal of the CT-peptide ... often in these cases, the need to remove these sequences to obtain full activation is explained by the role of C-terminal determinants in allowing proper secretion, correct folding or targeting...

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báo cáo hóa học: " Brain inflammation and oxidative stress in a transgenic mouse model of Alzheimer-like brain amyloidosis" pptx

Ngày tải lên : 19/06/2014, 22:20

... inflammation and Aβ levels in addition to the deposition of Aβ in brain [13,14] Despite this evidence, and the considerable theoretical and therapeutic interest, the relationship between brain inflammation ... amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis that oxidative stress and inflammation represent important but distinct therapeutic ... revision of the manuscript for intellectual content Domenico Praticò has been involved in the conception and design of the studies, interpretation of data, drafting and critical revision of the manuscript...

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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot

Ngày tải lên : 20/06/2014, 01:20

... nucleotide intervals Cell 20 00, 101 :25 -33 Vance V, Vaucheret H: RNA silencing in plants-defense and counter-defense Science 20 01, 29 2 :22 77 -22 80 Gitlin L, Andino R: Nucleic acid-based immune system: the ... alterations, ensuring the effective activity of the siRNAs described in the present study This work offers an insight into the use of RNAi in animal breeding for disease resistance The commercial ... on BHK -21 cell To further test the anti-FMDV activity of the siRNAs, we challenged Kunming White suckling mice (2 3 days old and weighing 3–4 g) The suckling mice were subcutane- Page of (page...

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Báo cáo y học: "Intra-articular injection of recombinant TRAIL induces synovial apoptosis and reduces inflammation in a rabbit knee model of arthri" pdf

Ngày tải lên : 09/08/2014, 07:20

... released into the culture medium and recovered by alkaline extraction were quantified using scintillation counting The value of saline control joints was assigned 100% and the results of the rTRAIL ... estimate the degree of TUNEL staining in synovial tissue sections Investigators blindly reviewed primarily two areas: synovial lining and subsynovial regions The extent of staining in these areas ... the synovium, intra-articular injection of exogenous TRAIL protein reduced the leukocytic infiltrate into the inflamed joints, indicating that TRAIL may inhibit joint inflammation The mechanism...

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báo cáo khoa học: "Therapeutic activity of two xanthones in a xenograft murine model of human chronic lymphocytic leukemia" doc

Ngày tải lên : 10/08/2014, 22:21

... transplantation of this cell line JOK-1 into SCID mice led to the establishment of a CLL model, allowing the evaluation of the antileukemic efficacy of fludarabine phosphate [9] Furthermore, we ... doses and time of treatment as well as different schedules of administration are in progress in order to improve the therapeutic efficacy of the two xanthones Studies of their exact mechanisms of ... design the study; PY designed the study; JPK designed the study, interpreted the data and revised the manuscript; CBi interpreted the data and wrote the manuscript All authors read and approved the...

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