exercise eating linkage mediated by neuro endocrine axis and the relevance in regulation of appetite and energy balance for prevention of obesity
Báo cáo khoa học: Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3 docx
Ngày tải lên :
30/03/2014, 11:20
... membrane-permeable inhibitor of cathepsins B, H, and L, and pepstatin A [24], an inhibitor of cathepsins D and E, for 24 h under nutrient-rich conditions [15,25] These results suggest that a modified form of ... modified form of Atg8L, accumulates in HeLa cells in the presence of inhibitors of lysosomal proteases, E64d and pepstatin A (A) Accumulation of Atg8L-II in the presence of E64d and pepstatin A Myc–Atg8L)3xFLAG ... substrate of these three enzymes involved in mammalian Atg8 conjugation Results The C-terminus of Atg8L is cleaved in HEK293 cells, and the Gly116 of Atg8L is essential for cleavage The C-termini of...
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báo cáo hóa học: " Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1" pptx
Ngày tải lên :
19/06/2014, 22:20
... redistribution and/ or activation of receptor, thereby increasing the cellular effects of IL-1b Modulation of PAK-1 signaling in cultured neurons and glial cells Neuronal and glial cells cohabit the CNS and ... 0.5 μCi of [ H] thymidine and incubated at 37°C, 5% CO2 for h Then, the spleens were homogenized and the resultant cell suspensions pooled in the presence or absence of Con A and seeded in triplicate ... particularly the hypothalamic pituitary-adrenal axis (HPA) and the hypothalamic-pituitary-gonadal axis, in the 1980s To date, IL-1b has remained the most studied inflammatory cytokine in the mediation of...
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Protecting the Elderly in Times of Disaster: The Critical Need for Comprehensive Disaster Planning and Exercise Design doc
Ngày tải lên :
28/03/2014, 20:20
... Lack of understanding of what comprises a “vulnerable population”, and the implication for this group prior to, and during, a disaster Area Lack of adequate understanding and training of the first ... preparing ourselves for this likelihood of Avian Influenza, and the possibility of Pandemic Influenza The potential for pandemic is still disturbingly real It is unfortunate that by being inundated ... Posed During Training Session There are several questions that are posed at the beginning our two day train -the- trainer sessions and conference intensives They are used to raise the awareness of issues...
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Báo cáo hóa học: " Reversal of TMS-induced motor twitch by training is associated with a reduction in excitability of the antagonist muscle" ppt
Ngày tải lên :
19/06/2014, 08:20
... performed, and the possibility of extending the training over time, is relevant in determining the optimal parameters to maximize the magnitude and duration of the observed effects The effect of ... Accuracy and consistency of the direction of training exercises were monitored in realtime by the investigators throughout the experiment Giacobbe et al Journal of NeuroEngineering and Rehabilitation ... and reduce cortical inhibition are therefore appealing for augmenting motor learning in behavioral therapies [38-40] Here we present data supporting the idea that depending on the nature of the...
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Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
Ngày tải lên :
16/02/2014, 09:20
... [10] During leptin-induced adipocyte apoptosis, the expression of PPARc is increased by 80% [10] Furthermore, the activation of PPARc stimulates apoptosis in a variety of cell types, including cancer ... However, in many other types of cell, the activation of PPARc is linked to the induction of apoptosis It has been suggested that PPARc is involved in the suppression of cell proliferation and promotion ... troglitazone at the indicated concentrations for 96 h or as indicated in the figure legends For the evaluation of apoptosis by AO ⁄ EB staining, the treated 3T3-L1 adipocytes were stained with 10...
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Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
Ngày tải lên :
18/02/2014, 08:20
... eliminating any possible effects as a result of the introduction of DNA within cells, and indicating that, in itself, the interaction of ODN with STAT3 induced these effects (i.e inhibition of the ... (particularly in whole animals) by impairing the action of STAT1-dependent interferon The results obtained in the present study, including the ability of the hairpin decoy STAT3 ODN to inhibit both ... The role played by STAT3 in malignant cell growth is mediated in part by the up -regulation of the expression of genes involved in cell survival and proliferation, including those for Bcl-xl, Bcl-2,...
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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
Ngày tải lên :
18/02/2014, 18:20
... contained 2.5 lg of total protein Values are the average of four samples with error bars indicating the SEM induced by treatment of the cells with the protein tyrosine phosphatase inhibitor sodium ... and kinase activity The N-termini of CDKs 1, 2, and are highly conserved and there is an equivalent residue to the Y17 site of CDK4 in each of these kinases To allow the study of CDK4 Y17 phosphorylation, ... of CDK4 by Src family kinases cell cycle arrest, leading to an increase in chromosomal aberrations and cell death, indicating that phosphorylation of this site is important for integrity of the...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... analysis and for the fever index of pyrogen test in rabbits As shown in Table 2, neither Wort nor HIMO affected the production of IL-1b or IL-6 in the SP The induction of the febrile response in rabbits ... dithiothreitol and lgÆmL)1 each of aprotinin, leupeptin, antipain, and pepstatin A), and incubated on ice for 20 The nuclear fractions were isolated by centrifugation at 11 000 g at °C for 10 s The obtained ... fluoride and lgÆmL)1 each of antipain, leupeptin, aprotinin, and pepstatin A) and then incubated for 20 on ice with occasional mixing The nuclear debris was removed by centrifugation at 12 000 g for...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
Ngày tải lên :
19/02/2014, 06:20
... of Ku and predominantly forms a large complex without any mobility shift in the well of the gel [31] The stability of the complex against salt is also enhanced by the presence of Ku Interestingly, ... S ⁄ MAR DNA of an anchor of chromatin loops that tend to transform into ssDNA by unwinding [38] and are potential sites for aggregation of S ⁄ MAR-binding proteins In this view, the direct association ... supernatants and repeatedly rinsed with reaction buffer, resulting in the removal of excess inhibitor For EJ, the aggregates were incubated with mm of ATP for h at 20 °C and terminated by incubation...
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Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf
Ngày tải lên :
21/02/2014, 00:20
... (lanes and 3) did not in uence the intracellular level of the scPs To quantify the inhibition of proteasome activity caused by the aggregating scFv fragments, we compared the scPs band (32 kDa) in ... Arrows point to the scPs, and the asterisks denote three ubiquitinated bands of the scPs Overloading the gel up to three times allowed us to highlight the scPs-ubiquitinated bands in lanes and (B) ... huntingtin, parkin and prion [22,24,25,28], results in aggresome formation and impairment of the UPS and, in some cases, apoptosis [24,28] For example, expression of polyglutamine-expanded huntingtin fragment...
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Báo cáo khoa học: Modeling of ATP–ADP steady-state exchange rate mediated by the adenine nucleotide translocase in isolated mitochondria potx
Ngày tải lên :
16/03/2014, 00:20
... protons into the matrix, bypassing F0 F1-ATPsynthase [6] The dotted line shows the result of the modeling after estimation of the unknown parameters The con- Modeling of ANT ditions of the described ... rate mediated by the ANT As also seen in Fig 3, the calculated values of the protonmotive force (PMF) in the presence of nigericin are higher than those in the absence of the ionophore The calibration ... in the same concentration range as in (A) Inset: a representative experiment showing the effect of the addition of cATR (in the concentrations indicated in the inset gure, in nM) on DWm, as indicated...
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Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx
Ngày tải lên :
16/03/2014, 02:20
... cells and HaCaT cells expressing nCLU (C120) were analyzed for the expression of CLU or b-actin In (E), the two faster-migrating bands corresponded to the minimal Ku70-binding domain of nCLU The ... signals in human and rodent cells can induce the production of various CLU protein isoforms, including nCLU [20] Second, the induction of nCLU and the reduction in sCLU expression may be linked ... overexpression of the c-fos oncoprotein suppressed the expression of the prosurvival psCLU and sCLU isoforms and induced nCLU, a cell death signaling protein [21,22,26–28] To further verify the effects of...
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Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
Ngày tải lên :
16/03/2014, 04:20
... analyzed by microscopy of DT40 and E2A)/) (Fig 2B) As expected, the viability of the two cell lines did not differ in the absence of PMA/ionomycin However, in the presence of PMA and ionomycin, the ... to the apoptotic induction of the DT40 cell line cooperatively mediated by E2A and BCR signaling, DT40 and E2A)/) were cultured in the presence of PMA/ionomycin, and RT-PCR was performed for ... in DT40 Thus, in E2A)/), in the presence of PMA/ionomycin, the time courses of alterations in the mRNA and protein levels of survivin were virtually similar up to 24 h, but the findings that the...
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Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx
Ngày tải lên :
16/03/2014, 06:20
... TGFb for 24 h in the absence or presence of the general inhibitor of transcription actinomycin D, and changes in the actin cytoskeleton were monitored by fluorescence using rhodamine phalloidin ... 1A) We then examined the effect of the TGFb signaling pathway in transcriptional regulation of the genes coding for the human Rho GTPases A and B by two different approaches: measuring the mRNA ... TGFb for 24 h in the absence or presence of actinomycin D (5 lgÆmL)1) Changes in actin cytoskeleton were monitored using rhodamine phalloidin staining (B,C) TGFb induces the transcription of the...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... [12] Therefore, binding of the iron regulator DmdR1 will interfere with RNA polymerase interaction and expression of the desferrioxamine cluster Indeed, binding of the pure DmdR1 protein to the ... box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desA expression by binding of DmdR1 to the iron box The same overlapping was found in S pilosus DmdR1 binds to the ... regulated by iron concentrations in the culture medium, and this regulation is mediated by binding of the regulatory protein DmdR1 to the iron box located in the promoter region of the desABCD...
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Relevant Topics in Eating Disorders Edited by Ignacio Jáuregui-Lobera ppt
Ngày tải lên :
17/03/2014, 02:20
... apparent increase was the rise in readmissions of women with anorexia In light of these findings, Williams and King argued that the idiom “epidemic” was inappropriate to describe the trend in the incidence ... categories of ED within communities It is important, however, to consider the presence of different profiles (e.g., purging in the absence of binge eating) and question the validity of the existing ... One of the most explored areas in AN research is the epidemiology, specifically, the estimation of the prevalence and incidence of the disease The incidence measures the strength of occurrence of...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
Ngày tải lên :
17/03/2014, 10:20
... the cells were incubated for another 24 h and then infected by Sendaı¨ virus for h Cells were washed and preincubated in a Met and Cys-depleted medium for h Metabolic labeling was performed by ... investigated the in uence of the ARE and the CRID on the poly(A) status of the human IFN-b mRNA in natural conditions of IFN-b synthesis Materials and methods Reagents All the reagents and enzymes used in ... in which the IFN-b gene contained or not the ARE (pIFNHA and pIFNHAAU–) In addition, the sequence encoding the HA epitope was inserted at the end of the IFN-b coding sequence to distinguish the...
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Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc
Ngày tải lên :
17/03/2014, 17:20
... Pereira for critical reading of the manuscript We would also thank Dr Francoise Moreau-Gachelin for providing the Spi-1 cDNA and Drs Yvan de Launoit and Laurent Coutte for providing the ERM, ETV1 and ... within )490 and )408 (ETS-RE) were then named A, B and C, in the order of 5¢ to 3¢ Point mutations (TTCC to TTCT) were introduced on each or combinations of the three putative sites Mutations in ... not by an unrelated antibody (lane 13) Increasing amounts (10 and 100-fold molar excess) of unlabeled site C oligos were able to compete for the binding of Ets-1DAE to the Ets-1 consensus binding...
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Báo cáo khoa học: A large complex mediated by Moc1, Moc2 and Cpc2 regulates sexual differentiation in fission yeast ppt
Ngày tải lên :
23/03/2014, 05:22
... described in Experimental Procedures The screened genes were verified by reintroducing them into the test strain AH109 and the genes cloned in the pGAD vector were identified by sequencing The results of ... indicated by ‘+’ and a negative signal by ‘)’ The strength of blue color on the X-gal filter is shown by the number of plus marks Gal4-BD, GAL4 DNA-binding domain Moc1 interacting proteins Systematic ... indicated by ‘+’ and a negative signal by ‘)’ The strength of blue color on the X-gal filter is shown by the number of plus marks Gal4-BD, GAL4 DNA-binding domain Moc2 interacting proteins Systematic...
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Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot
Ngày tải lên :
23/03/2014, 06:20
... cells were preincubated for h with or without the pankinase inhibitor staurosporine at the concentrations indicated, and treated with 0.5 M sorbitol for 60 min, and then incubated for h in sorbitol-free ... gain a better understanding of the role of hDlg phosphorylation in the adaptation of cells to stress, by determining whether stress could regulate hDlg degradation in cells exposed to changes in ... activation of these proteases, the activation of caspase being transient and the activation of caspase being stronger and sustained over time In addition, the length of activation of caspase...
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