equipment for animal laboratories and holding areas
CRC handbook of laboratory Safety - Chapter 4 docx
Ngày tải lên :
11/08/2014, 04:20
... the potential for fire, explosion, and reactivity Known acute and chronic health effects and related health information This information is to include signs and symptoms of exposure and any medical ... above) Precautions for safe handling, including protective measures during repair and maintenance of apparatus employed in using the equipment and procedures for cleanup of spills and leaks Relevant ... results The following information will aid in the selection of appropriate vendors and provide criteria for selecting “clean” animals Selection Criteria for Rodents and Rabbits Animals purchased from...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
Ngày tải lên :
23/03/2014, 03:20
... 115–132 159–176 220–238 Forward Forward Forward Reverse Reverse Forward Forward Reverse Forward Reverse 551–569 532–551 620–639 571–591 642–661 a In cases of the same primer for several trypsin variants, ... on total cDNA as follow: one cycle at 94 °C for min, 35 cycles at 94 °C for 30 s, 50 °C for 30 s, 72 °C for min, and one overextension cycle at 72 °C for 10 Lack of genomic DNA contamination was ... Intermoult animals were placed on ice for 10 to obtain a chill coma and were then dissected to collect the digestive gland, stomach, intestine, gills, heart and abdominal muscle Before dissection,...
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Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx
Ngày tải lên :
23/03/2014, 04:21
... atom and protein atoms have been observed between the protein and the ligand (Table 3) The second lactose molecule is held near b-strands b7, b8 and b9 (Table 3) At this position, lactose forms ... ligand The crystals of the native protein were soaked in solutions containing ascorbic acid and lactose separately for 48 h and the crystal structures of the two complexes were determined and ... near the b-strands, b4 and b5, and the interactions involve residues AspA151, GlyB165 and LysB166 At least six hydrogen bonds Molecule O1¢ O2¢ O6¢ O1 O2 O4 a The water molecule forms a bridge...
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Báo cáo sinh học: " Rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene I" pdf
Ngày tải lên :
18/06/2014, 18:20
... the cells for adsorption for 45 at 37°C and then washed with media to remove unbound virus At different time points post-infection (p.i.), cells were lysed in RIPA buffer and assayed for protein ... Myc-RIG-I, GFP-SA11 NSP1, and GFP separately Immunoprecipitation and Western blot analysis were performed as described for panel A (C) Interaction between NSP1∆IRF3BD of rotavirus SA11 and RIG-I Transfection, ... Rotaviruses were detected by RIG-I and/ or MDA-5 when they enter host cells Then RIG-I and/ or MDA5 associate with MAVS/IPS-1, and antiviral signals are propagated to IRF3 and NF-κB Rotavirus NSP1 mediates...
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báo cáo hóa học:" Arthroscopic debridement of the osteoarthritic knee combined with hyaluronic acid (Orthovisc®) treatment: A case series and review of the literature" docx
Ngày tải lên :
20/06/2014, 01:20
... HA delivery and arthroscopic debridement for OA of the knee utilizing health related questionnaires (WOMAC and SF-36) Methods and materials This investigation was approved by and performed in accordance ... Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Short Form-36 Physical Component Summary questionnaires (SF-36 PCS.) Between May and July of 2006, a single surgeon (BB) performed ... necessary and were given a two week prescription for narcotic analgesics (Vicodin 5/500 mg) for pain control Patients were asked to ingest one enteric coated aspirin per day for two weeks for DVT...
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báo cáo hóa học:" Stabilization of mid-sized silicon nanoparticles by functionalization with acrylic acid" pptx
Ngày tải lên :
21/06/2014, 17:20
... Acknowledgments We thank Anna Elsukova and Zi-An Li for providing the TEM pictures and Alice Sandmann (all University of Duisburg-Essen) for her support with the DLS and Zeta-potential measurements ... carried out the FTIR and DLS measurements, drafted the manuscript, and participated in the design of experiments HK and HN carried out the XPS measurements and interpretation and contributed to ... nature of the functionalization and to compare the as-prepared and functionalized silicon nanoparticles, TEM measurements were performed Figure shows the particles before and after surface treatment...
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Titration with Oxalic Acid ppsx
Ngày tải lên :
07/07/2014, 00:20
... Place the buret and buret stand on the floor if necessary Acids are corrosive and bases are caustic Wipe up spills and drips immediately with wet towels, and the dry Wash affected skin areas with ... and notify your teacher Burets are cumbersome and break easily – handle them with care Procedure Part A Preparation Put your goggles on While one lab partner prepares the oxalic acid (Steps and ... buret to the support stand Obtain 50-60mL of sodium hydroxide (NaOH) solution, unknown, in a 100 mL beaker Position the buret so that the top is below your eye level and place and empty beaker under...
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Báo cáo y học: "Safety and Effectiveness of two treatment regimes with tranexamic acid to minimize inflammatory response in elective cardiopulmonary bypass patients: a randomized double-blind, dosedependent, phase IV clinical trial." pdf
Ngày tải lên :
10/08/2014, 09:22
... responsible for the statistical analysis, data interpretation, and drafting the manuscript MB, LL, RP and MLM, were responsible for data collection and processing blood simples during the study and provided ... useful suggestions PM and JMR, was responsible for determination of coagulation-fibrinolysis parameters and interpretation JMB and BM, was responsible for determination of IL-6 and interpretation ... either TA 40 mg/Kg before CPB and placebo after CPB (TA1) or TA 40 mg/Kg before and after CPB (TA2) after heparin reversal The code was revealed once recruitment, data collection, and laboratory analyses...
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Báo cáo khoa học: " Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution" pot
Ngày tải lên :
12/08/2014, 04:21
... both Galliformes and Anseriformes and belonged to different HA subtypes At least three repeats were conducted for each explant system and each virus subtype After 24 hours of culture and one washing ... of viral attachment (PVA) of human and AIVs in pig tissues, we focused on the expression in epithelial cells and glands of NE, TE and BE and in bronchioles and alveoli of LE An overview of the ... tropism [38], Wan and Perez [16] have suggested a dual receptor specificity (for both human- and avian-like receptors) for A/New Caledonia/20/99 (H1N1) and a strict Siaa2-6 preference for A/Panama/2007/99...
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Báo cáo y học: "Conventional or physicochemical approach in intensive care unit patients with metabolic acidos" ppsx
Ngày tải lên :
12/08/2014, 19:22
... circulation during sepsis and liver failure, and may be a variety of organic and inorganic compounds [8] The use of urealinked polygelines, for example, as the priming fluid for the extracorporeal ... concentration > 110 µmol/l for males and > 100 µmol/l for females All patients were followed up to determine the 28-day survival Results are reported as the mean ± standard deviation or the median ... Table 1, and acid–base and electrolyte data for the study population are presented in Table Twenty-nine patients had evidence of a decreased renal function Urine samples were positive for ketones...
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Báo cáo khoa học: "Circulating anions usually associated with the Krebs cycle in patients with metabolic acidosis" potx
Ngày tải lên :
12/08/2014, 22:22
... collected patient samples and drafted the manuscript WM developed and performed the enzyme assays, participated in the design of the study, performed the statistical analysis and helped to draft the ... likely source for the generation of these observed anions is the mitochondria, we have no direct evidence for this and the results may indeed reflect compartmentalised oxidative and glycolytic ... the study, performed the initial mass spectrometry and helped in assay development DFT collected patient samples and participated in study design JMP helped in assay development and stability...
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Báo cáo y học: "Oxygen consumption is depressed in patients with lactic acidosis due to biguanide intoxication" docx
Ngày tải lên :
13/08/2014, 20:21
... (mM) Metformin 70 6.4 7.21 22 Metformin 63 12.4 6.95 33 Metformin NA 10.8 6.76 21 Metformin NA 15.2 7.06 18 Metformin NA 9.0 6.63 21 Metformin NA 10.3 6.82 21 Metformin NA 10.8 6.70 24 Metformin ... participated in its design and coordination, performed the statistical analysis and drafted the manuscript RR, PT, and SV participated in study design and data collection MS, AR, and GF participated ... CI; SvO Metformin NA 13.2 6.79 21 CI; ScvO2 63 S 10 Metformin NA 4.7 7.13 19 CI; ScvO2 66 S 11 Metformin 53 4.5
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Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation
Ngày tải lên :
02/09/2015, 13:46
... activity (Noorulla and Sreenivasulu, 2011; Singh et al., 2010; Freeman, 1973; Che and Siu, 2010; Podunavac-Kuzmanovic and Cvetkovic, 2007) and an amino acid as a secondary ligand were significant ... sterilized petri-dishes and allowed to stand for some minutes, then a cork-borer with a diameter of 12 mm was used to bore uniform holes on the surfaces of the dried agars and into each hole was ... in mm and average zone inhibitions were determined Triplicate data were taken for the calculation of mean inhibition Results and discussion 15.3 lS cmÀ1 and 4b is 8.20 lS cmÀ1 while 4c and 4d...
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Enhanced transdermal delivery with fatty acids and electroporation
Ngày tải lên :
12/09/2015, 10:37
... containing PHY formulation 43 Preparation of CsA and [D-Ala6]-LHRH formulation 43 H NMR spectroscopy for PHY and fatty acid 44 Conductivity measurement 44 Formulation treatment for SC ... DECANOIC AND LAURIC ACID WERE NOT PERFORMED AS THEY EXIST IN SOLID FORM AT ROOM TEMPERATURE 61 FIGURE 5.6 REPRESENTATIVE FTIR SPECTRA OF THE SC BEFORE AND AFTER DELIPIDISATION, AS WELL AS AFTER FORMULATION ... 2000) Therefore it is important to design a suitable sustained delivery system for CsA and for diseases such as psoriasis and atopic dermatitis, topical CsA has displayed superior efficacy and reduced...
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AN0559 optimizing serial bus operations with proper write cycle times
Ngày tải lên :
11/01/2016, 11:51
... system certification for its worldwide headquarters, design and wafer fabrication facilities in Chandler and Tempe, Arizona in July 1999 The Company’s quality system processes and procedures are ... in systems for two purposes: storing data and reading back data Read operations are at full clock speed, so the only methods for optimization are to run the clock at maximum frequency and to utilize ... QS-9000 compliant for its PICmicro® 8-bit MCUs, KEELOQ® code hopping devices, Serial EEPROMs and microperipheral products In addition, Microchip’s quality system for the design and manufacture...
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The Transformation of Escherichia coli with Deoxyribonucleic Acid isolated from Bacteriophage Adgt
Ngày tải lên :
28/05/2016, 11:25
... picked and grown in Hvglucose liquid medium and a lysate prepared by u.v induction The lysates were assayed for their plaque-forming and transducing activities using the assays for> ' and >'dg ... transforming activity is very close to that observed for Hemophilus transforming DNA (Zamenhof, Alexander & Leidy, 1953) Moreover, just as was observed for Hemophilus DNA, the decay of transforming ... transforming principle and of )'dg DNA varies with recipient cell concentration The figure for transforming principle is for H influenzae at 1·6 X 10 cells per ml (Goodgal & Herriott, 1957) and...
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Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding specificity pdf
Ngày tải lên :
16/02/2014, 14:20
... strands bB and bF, and, in fact, they have been labeled bB1 and bB2, and bF1 and bF2, respectively Two hundred and two hydrogen bonds among protein atoms stabilize the 3D structure The b-barrel forms ... HP1286 b-Strands, a-helices and turns are in yellow, red and green, respectively Strands are labeled from A to H Strands B and F, owing to some irregularities, are divided into two parts and labeled ... strands Some of the strands present some kinks that break the continuity of the hydrogen bond patterns, and so they are formally considered to be composed of two parts This happens for strands...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
Ngày tải lên :
18/02/2014, 17:20
... , kp ¼ kk22þk33 and the equilibrium associak1 ð tion constant for the product Ki ¼ kkÀ3 Although the Km and the kp derived for Model I and Model II have different algebraic form, their meaning ... substrates Materials and methods Materials Sodium salt and free acid forms of oleic, stearic and arachidonic acids were purchased from Sigma-Aldrich Kft (Budapest, Hungary) and stock solutions ... P for any combination of Km, kp and Ki and therefore it has an inverse function P = PM(t, Km, kp, Ki, S0, Et0), which can be estimated numerically using the table look-up procedure described for...
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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
Ngày tải lên :
20/02/2014, 02:21
... stearothermophilus [47] and T brucei) are summarized in Table With all mutants, and for both organisms, a decrease in kcat for the forward reaction was observed For T brucei, however, and unlike B stearothermophilus ... constants found for compounds 5, and were in the range of the Km values for 1,3-BPGA and even up to three times lower for compound Selection of T brucei gGAPDH residues to be mutated and measurement ... by sequencing before they were introduced into Escherichia coli for gene expression Overexpression and purification of wild-type and mutant T brucei gGAPDH T brucei wild-type and mutated gGAPDH...
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