... reproduction
eg Candida, Cryptococcus
RNA viruses: Influenza
Nature andProperties
of Micro-organisms
Laura JV Piddock
Viruses
Obligate intracellular parasites
Use host cell for nucleic acid and ... invasiveness of
pathogenic bacteria by reducing opsonisation and
protecting from phagocytosis.
E.g. Streptococcus pneumoniae in RTI
vaccination: pneumococcal; meningococcal A and C
GLYCOCALYX ... conjugation
(transfer of plasmid DNA)
Prions
Infectious protein
No DNA or RNA
Normal cellular form of protein vs.
pathological form
Pathological form
found in neural
tissue and tonsils
PrP-C...
... somewhat expand.
The dosimetric propertiesof the powder were studied. In the TL glow curve of LiF:Mg,Cu,Na,Si
powder the main dosimetric peak appeared in the range of 227-247
o
C and the ratio of the ... powder were
studied. The main dosimetric peak of LiF:Mg,Cu,Na,Si appeared in the range of 227− 247
o
C and
the ratio of the height of the main peak to that of the other small peaks in the LiF:Mg,Cu,Na,Si
is ... with rate of 4 l/min. After annealing treatment, the samples were washed with HCl solution.
VNU Journal of Science, Mathematics - Physics 23 (2007) 225-231
Preparation and characteristics of LiF:Mg,Cu,Na,Si
thermoluminescent...
...
concentration of the SnCl
4
solution, and the films were annealed at different temperatures and for
different period of time.
Photoconductivity measurement
In order to evaluate propertiesof obtained ... (R
D
) and the light resistance (R
L
) under the irradiation of 7W Hg lamp at
the distance of 10cm were measured. The calculated ratio of R
D
/R
L
was considered as a
photoconductivity of obtained ... results of measurements show that all values R
L,
R
D,
and
R
D
/R
L
strongly depend on the temperature and time of annealing as presented in Fig. 4.
P.V. Nho, T.K. Cuong / VNU Journal of Science,...
... Identi®cation of the sipV(ba) and sipW(ba) gene regions of B. amyloliquefaciens. (A1/B1) PCR reactions with genomic DNA of B. amy-
loliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and ... PstIandEcoRI in case of sipV or EcoRI-SacIincaseofsipW and
ligated into corresponding sites of pUC18 DNA. The ligation mixes were used for PCR with oligo nucleotides Uni1 or Uni2 and pairs of ... 2002
Identi®cation andpropertiesof type I-signal peptidases
of
Bacillus amyloliquefaciens
Hoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer
and JuÈ rgen Hofemeister
Institute...
... the
K
m
and k
cat
values of E. coli Fre for riboflavin are
reported as 2.5 lm and 52.4 s
)1
with NADPH as the
electron donor and 1.3 lm and 30.6 s
)1
with NADH
[13]. For FMN, K
m
and k
cat
values of ... twofold axis associated with the
dimer passes through the vicinity of the side chains of
Pro13, Ile126, Gln133 and Ile163. The corresponding
Table 1. Summary of x-ray data collection, phasing and ... the case of A. vinelandii ferric reductase, the enzyme
was purified as a mixture of two proteins or a hetero-
dimer with molecular masses of 44 600 and 69 000 Da
[25]. Although none of the amino...
... decomposition of tetra-
thionate was proposed by Steudel et al.[16],Hazeuet al.
[17] and Meulenberg et al. [18] in experiments with intact
cells of A. ferrooxidans and A. acidophilum.Propertiesof
purified ... m
M
NADP
+
(Sigma)
and enzyme. The production of NADPH was determined
spectrophotometrically at 340 nm. One unit of Glc6P
dehydrogenase was defined as the amount of protein that
reduced 1 lmol of NADP
+
in ... enzyme may be
due to several reasons. Copper and several other divalent
ions stimulated the activity of the tetrathionate hydrolase
and they and other cofactors may be present in the crude
enzyme...
... lombricine (30% of that of taurocyam-
ine) and glycocyamine (7% of that of taurocyamine). Neither TK catalyzed
the phosphorylation of creatine. Comparison of the deduced amino acid
sequences of mitochondrial ... 3381–3385.
Cytoplasmic and mitochondrial taurocyamine kinases K. Uda et al.
3530 FEBS Journal 272 (2005) 3521–3530 ª 2005 FEBS
Origin andpropertiesof cytoplasmic and mitochondrial
isoforms of taurocyamine ... correlations of this relaxed sub-
strate specificity and the possibility of cytoplasmic and
mitochondrial isoforms of TK, we have amplified two
cDNAs coding for Arenicola brasiliensis TKs and
cloned...
... mixture of
0.05
M
NaP
i
buffer, pH 3, and acetonitrile (6 : 1, v/v);
separation was performed isocratically, at a flow rate of
1mLÆmin
)1
, and the volume of injected samples was 10 lL.
The amounts of ... consists
actually of a unique enzymatic protein.
Molecular mass and pI determination of F 4¢-OMT. The
molecular mass of the pure enzyme was calculated both
through gel-filtration and PAGE (Fig. 7) ... the method of Baayen and
Elgersma [13], with a 500-lLdropofFod conidial
suspension at a concentration of 9 · 10
6
conidiaÆmL
)1
;20
additional plants, inoculated with a 500-lL drop of double
distilled...
... lg
of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an
[a-
32
P]GTP overlay [17] of a nitrocellulose blot of a gel containing
40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rab
proteins, ... GST±Rab31 and
Fig. 7. Puri®cation andproperties o f wild-type and mutant Rab31 and
Rab32 expressed a s GST±fusion proteins. Samples of platelet particu-
late fraction protein andof puri®ed GST and ... expression andpropertiesof Rab31
and Rab32
New blood platelet Rab proteins
Xiankun Bao
1
, Andrea E. Faris
2
, Elliott K. Jang
1
and Richard J. Haslam
1,2
Departments of
1
Pathology and Molecular...
... by MALDI-
TOF mass spectrometry to be 51.0 kDa and it had pI values
of 3.6, 3.9 and 4.4. The molecular mass of wolffish kininogen
was 45.8 kDa and it had pI values of 4.1, 4.3, 4.35 and 4.4.
Partial ... Kalkkinen
1
1
Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland,
2
Norwegian College of Fishery Science,
University of Tromsø, Norway,
3
Institute of Medical Biology, ... present in the MALDI-
TOF mass maps from the 60 kDa band were also present in
the mass map from the 78-kDa band, suggesting that the
60-kDa band represents a fragment of the 78-kDa band.
Similar related...
... been synthesized using a novel Fe
3
O
4
nanoparticles catalyst.
The lengths of SiOx nanowires are in the range of several tens to hundreds of micrometers, and the diameters
of nanowires are 20–80 ... Preparationand photoluminescence of high density SiOx nanowires with Fe
3
O
4
nanoparticles catalyst
X.J. Wang
a,
⁎
, B. Dong
b
, Z. Zhou
a
a
School of Electronics Engineering and Computer ... assays, chemical sensors, and
superparamagnets [8,9]. Recently, efforts have been devoted to
preparation of Fe
3
O
4
nanoparticles, nanowires and nanotubes. In
2002, Sun and Zeng [10] prepared the...
... kDa band in T cells. A
Cb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells. (C) Lin-
ear depiction of Ca1, Cb1 and ... p50
csk
[16] and Raf-1 [17] in
the early phase of the stimulatory process and regula-
tion of interleukin-2 production through phosphoryla-
tion of the nuclear factor of activated T cells [18] and
nuclear ... Jahnsen
2
and Bjørn Steen Ska
˚
lhegg
1
1 Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
2 Department of Biochemistry, Institute of Basic...
... The effect of geometrical parameters and material propertiesof face
sheets and core are studied on the face wrinkling of sandwich plates. Comparison
of the present results with those of plate ... anisotropic propertiesof strength and stiffness of masonry in horizontal
and vertical direction, tensile and shear stiffness of cracked masonry,
• Concrete yielding in compression, opening of cracks ... tension,
mechanism of opening and closing of cracks in concrete under dynamic load,
tensile and shear stiffness of cracked concrete,
• Strain rate effect of the material propertiesof masonry, reinforced...
... expression andpropertiesof the recombinant enzyme
Y. T. Pan
1
, J. D. Carroll
2
and A. D. Elbein
1
1
Department of Biochemistry and Molecular Biology and
2
Department of Microbiology and Immunology, ... the cloning and
expression of the M. tuberculosis tps gene in E.coli,andthe
production of active TPS in these cells. The propertiesof the
recombinant enzyme have been determined, and are com-
pared ... starting at position 397 of the M. tuber-
culosis TPS, and another sequence of about 16 amino acids
starting at position 421 of the M. tuberculosis TPS.
Isolation and purification of recombinant TPS
To...