dna damage and apoptosis in fetal and ovarian reserve oocytes
Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf
Ngày tải lên :
09/08/2014, 09:20
... late toxicity in our series This finding opens the possibility to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new ... fractionation) and in the methodology used (PFGE, comet assay, gamma-H2AX induction) could help to explain the discrepancies observed Initial DNA damage levels in breast cancer patients Radiation-induced apoptosis ... the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of the study and in drafting the manuscript and has given final approval of the version...
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Báo cáo y học: " Upregulation of Bax and Bcl-2 following prenatal cocaine exposure induces apoptosis in fetal rat brain" doc
Ngày tải lên :
08/08/2014, 17:20
... cocaine on fetal brain apoptosis Assessment of nuclear chromatin morphology by Hoechst 33258 staining indicated that cocaine increased condensed and segmented apoptotic nuclei in the fetal brain ... to changes in Bcl-2 family protein levels in fetal brain, we examined the Bcl-2 and Bax protein expression As shown in Figure 4, Bax protein was minimally detected in control fetal brain However, ... gels in fetal rat brain Cocaine was administered subcutaneously to the pregnant rats for days as described in Methods Cocaine-induced nucleosomal DNA fragmentation in fetal brain was separated in...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
Ngày tải lên :
28/06/2014, 05:20
... al., 2005a) and its differential binding to DNA is a determining factor in its involvement in DNA replication, exhibiting distinct origin DNA binding properties from its association with DNA ends ... eukaryotic single-stranded (ss) DNA binding protein and it is required for DNA replication, recombination and repair RPA helps recruit DNA primase/polymerase α to the origins, stabilizing ssDNA in the ... both Dna2 and Fen1 in vitro in an ATPindependent manner (Kang et al., 2009) Stimulation depends on the specific protein-protein interaction between the N-terminal domain of Dna2 and Mph1 Since...
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Báo cáo y học: "Endothelin-1 enhances fibrogenic gene expression, but does not promote DNA synthesis or apoptosis in hepatic stellate cell" doc
Ngày tải lên :
13/08/2014, 13:20
... or Fas-ligand [19] were used to induce apoptosis in HSC In serum deprivation apoptosis, control was made with 10% serum In Fas-ligand induced apoptosis, control was run without Fas-ligand There ... FCS containing μg/ml of Fas enhancer (mouse IgG) and 10 to 50 μg/ml Fas-ligand for 24 h To investigate the influence of ET-1 on Fas-ligand induced apoptosis, HSC were cultured with increasing concentrations ... fibrogenesis and in particular in HSC matrix production and apoptosis remains controversial Therefore we examined cell proliferation, apoptosis and extracellular matrix metabolism of ET-treated HSC in...
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báo cáo hóa học:" Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)" pptx
Ngày tải lên :
20/06/2014, 07:20
... cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair [10] We determined if NaAsO2 and hyperthermia modulated the expression of XPC, a platinum -DNA damage ... human ovarian cancer cells were the kind gift of Dr Eddie Reed Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, 100 μg/mL penicillin/streptomycin, mM L-glutamine and ... Huinink WW, Beijnen JH, Bartelink H, Begg AC: Intraperitoneal cisplatin with regional hyperthermia in advanced ovarian cancer: pharmacokinetics and cisplatin -DNA adduct formation in patients and...
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Báo cáo khoa học: "Radiation induced apoptosis and initial DNA damage are inversely related in locally advanced breast cancer patients" ppt
Ngày tải lên :
09/08/2014, 09:20
... initial DNA damage was completed in the University of Granada (Spain) Initial radiation-induced DNA damage in PBLs was measured as previously described [18] and was considered an individual indicator ... defined by two constants: the coefficient in origin a (as the origin of the curve in the Y axis determining the spontaneous apoptosis) ; and the coefficient b defining the slope of the curve a and ... and has been involved in type of packaging likewise in the submission process PCL has been involved in conception and design of the study and in drafting the manuscript and has given final approval...
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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx
Ngày tải lên :
18/02/2014, 16:20
... protective effects against hypoxia and reoxygenation This is mainly ascribed to its ability to maintain redox status [17], intervening in the DNA repair system [18–20] and restoring antioxidant capacities ... detection of single-strand and double-strand breaks Pyruvate reduces DNA damage during ⁄ after hypoxia Cells cultivated in Petri dishes (25 mm in diameter) were suspended in 0.5 mL of NaCl ⁄ Pi, and cell ... reoxygenation but is ineffective at maintaining glutathione levels during hypoxia Glutathione is one of the main antioxidant compounds in the cell, and it is also essential for DNA synthesis and repair...
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Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt
Ngày tải lên :
16/03/2014, 00:20
... the cell nucleus and the key molecules p53, p73 and homeodomain-interacting protein kinase (HIPK2) Multiple functions of p53 in DNA damage- induced apoptosis The tumour suppressor and transcription ... an increased SUMOylation and stability of PML, suggesting an additional role of HIPK2 in regulating DNA damageinduced cell death Collectively, these findings indicate that HIPK2 is involved in DNA ... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated...
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in situ detection of dna damage, methods and protocols
Ngày tải lên :
11/04/2014, 09:47
... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage ... DNA making it ideal for labeling fragments in apoptotic cells The enzyme will also label single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in ... used methods for detecting DNA damage in situ is TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining (1) TUNEL staining was initially described as a method for staining cells that have undergone...
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báo cáo hóa học:" CCR9 interactions support ovarian cancer cell survival and resistance to cisplatin-induced apoptosis in a PI3K-dependent and FAK-independent fashion" pdf
Ngày tải lên :
20/06/2014, 07:20
... for 15 minutes After washing in PBS three times, the cells were incubated with 0.05% Tween-20 in PBS for 15 minutes After washing in PBS, the cells were incubated with TdT end-labelling cocktail ... growth inhibition of OVCAR-3 and SKOV3 cell lines caused by cisplatin in a CCR9-dependent fashion CCL25-induced cisplatin resistance of OvCa cell lines Treatment of OVCAR-3 and SKOV-3 cell lines ... Annexin V-FITC and propidium iodide (PI) staining Living cell populations are clustered in the Q3 quadrant; cells in early apoptosis are in the Q4 quadrant; late apoptotic/necrotic cells are in...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
Ngày tải lên :
09/08/2014, 09:20
... zvd-fmk By inhibiting apoptosis, we were able to reduce spontaneous DNA breakage and estimate rejoining as the initial DNA released minus residual DNA released without the interference of apoptosis ... radiation induced DNA breakage, which is attributable to rejoining, continued diminishing (Figure 5) The blockade of apoptosis thus allowed us to efficiently determine rejoining without the inference ... by apoptosis (which can be inhibited by caspase inhibitors), and 3) reducing the interference of spontaneous breakage improves our ability to estimate DNA repair and to determine apoptosis intensity...
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Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
Ngày tải lên :
09/08/2014, 09:20
... design DNAdirected alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA- directed ... then washed in PBS twice, and the anti-gH2AX antibody was added at a dilution of 1:500 in 1% BSA and incubated overnight at 4℃ Again, the cells were washed twice in PBS before incubating in the dark ... produce DNA interstrand and/ or intrastrand cross-links [29,30] As has been known, bifunctional alkylating agents induce collapsed replication forks that can lead to either cell cycle arrest, DNA...
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Báo cáo y học: " Physical and in silico approaches identify DNA-PK in a Tax DNA-damage response interactome" pot
Ngày tải lên :
13/08/2014, 05:21
... certain types of damage induced in DNA, principally interstrand crosslinks [20] In contrast, Cluster includes key genes whose proteins mediate cell cycle arrest in response to genotoxic and other cellular ... Tax-binding proteins generated from our physical mapping efforts, DNAPK was in the top five best represented binding proteins and occupied a top tier ranking via our functional clustering for DNA ... 278(39):37736 Defining first neighbor interactions of the known Taxbinding proteins In this section we conducted a query for immediate binding partners of a selected group of known Tax-binding proteins Our...
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Báo cáo y học: "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export" pps
Ngày tải lên :
13/08/2014, 06:20
... shuttling in response to DNA damage may be a consequence of their inability to be ubiquitinated Lysine residues 280 and 284 are ubiquitinated in response to DNA damage Since Tax is ubiquitinated in ... demonstrate that DNA damage results in increased mono-ubiquitination of Tax Mutation of Tax amino acids known to be targets of ubiquitination (K280 and K284) inhibited DNA damage- induced ubiquitination ... cytoplasm following DNA damage To examine this possibility, the distribution of wild-type Tax and a Tax mutant containing arginine substitutions in all lysine residues (TaxK1-10R) was examined by immunofluorescent...
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Regulation of spindle behaviour by DNA replication and damage checkpoints in budding yeast
Ngày tải lên :
14/09/2015, 14:03
... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage ... damage checkpoints, and the G2/M DNA damage checkpoint The G1 and G2 checkpoints respond to some types of localized physical damage to the DNA and are known as DNA damage checkpoints In S.cerevisiae, ... which recognize and bind to replication origins only during stages of low Cyclin-Cdc28 activity (i.e during G1), and permitting the initiation of replication only during high Cyclin-Cdc28 activity...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis tissue, and this effect in mtDNA is associated ... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli extract The Hp and E...
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Checkpoint and Coordinated Cellular Responses to DNA Damage
Ngày tải lên :
25/10/2013, 21:20
... that begins to explain how different processes involved in the maintenance of genomic stability are integrated and coordinated by the checkpoint pathway Sensing DNA Damage and DNA Replication Stress ... of DNA replication in telomere regions, or the function of telomerase Checkpoint and Coordinated Cellular Responses to DNA Damage 81 Interplay between Checkpoint Signaling and Chromatin In eukaryotic ... Yang · L Zou Checkpoint and Coordinated Cellular Responses to DNA Damage 71 nize certain damage- induced DNA structure and recruit the 9-1-1 complex in a manner similar to the loading of PCNA by RFC...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
Ngày tải lên :
16/03/2014, 01:20
... Jiang et al The ATP-binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore, ... no direct interaction between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the peptide-binding domain, was essential for this specific function of the protein Recent ... ATP-binding domain of HSP70 inhibits Smac release pcDNA3.1 HSP70-1 HSP70-2 HS A No direct interaction between HSP70 and Smac HSP70 Because HSP70 inhibited the release of Smac and apoptosis induced...
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Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc
Ngày tải lên :
22/03/2014, 16:20
... the WT protein wherein the side chain amino group of K182 residue in each subunit is involved in H-bonding with O6 and N7 atoms of G13 and with the N7 atom of A12 in the DNA strand In silico analysis ... apparently periodic interval (indicated by arrowheads in Fig 2A) The results of DNase I footprinting and TSP mapping indicate that DevR interacts cooperatively with the P and S sites and that the )35 ... Fig DNase I footprinting (A) DNase I footprint of WT DevR protein (0.5 lM and 1.0 lM concentration) and fdxA promoter DNA containing WT ⁄ C8G ⁄ T9A mutant P box DNA sequencing ladder of the same...
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Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt
Ngày tải lên :
22/03/2014, 21:20
... cells, including those in myeloid leukemia, and breast, prostate, colon, skin and brain cancer [4– 8] Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was originally discovered in 1997 ... and apoptosis was evaluated (D) *P < 0.05, **P < 0.01, (n = 3) participated in the VD3-induced apoptosis in gastric cancer cells indicated that vitamin D induced PTEN expression through binding ... vector, using the Lipofectamine 2000 (Invitrogen) protocol Cells were maintained in IMDM supplemented with 10% fetal bovine serum, 100 mgÆmL)1 penicillin, 100 mgÆmL)1 streptomycin, and 1000 lgÆmL)1...
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