... digesting the fragment with HindIII and
Bgl II andthe plasmid with HindIII and Bam HI. The
fragment was ligated into the plasmid andthe integrity of
the insertion was validated by digesting the plasmid
construct ... at
positions 597, 641, 667, and 691.
To construct the GST fusions ofthe tandem SH2 domains
of each SHP-1 and SHP-2, the sequences corresponding to
amino acids 2–232 of each SHP-1 and SHP-2 were
amplified ... with Eco RI and Bbr PI, then gel purified
(< 820 bp). The insert was then ligated into the pSPORT
vector andthe resulting full-length siglec-10 clone was
designated 995-2 andthe sequence...
... received from the hands of John Stark her brother Jonathan
and her sister Nancy, and heard ofthe death of her mother andof her brother Franklin at Starved Camp. That
CHAPTER XV 54
to these poor ... enter the building, and as they were slow in making their trades,
we had a good chance to see them all. The men, the boys, and most ofthe women were dressed in fringed
buckskin suits and their hands ... paling; and we children planted
Castilian rose bushes at the head and foot ofthe mound, and carried water to them from the house, and in time
their branches met andthe grave was a bed of fragrant...
... see that they meet at a point on the
circumcircle of ABC. The points A
2
, B
2
, C
2
are the reflections of this point in
the midpoints ofthe sides of ABC. Therefore, the triangles ABC and A
2
B
2
C
2
are ... 4.
Proof. It suffices to apply homothety of center the centroid of ABC and coeffi-
cient −
1
2
to the configuration ofthe previous claim.
Statement 5. Let O, I be the circumcenter and incenter of ... respectively. Then
the circumcenters ofthe triangles IAA
1
, IBB
1
, ICC
1
are collinear.
Proof. Applying an inversion of center I andthe previous assertion we see that
the circumcircles ofthe three...