... state may be
maintained mainly by key amino acids. In this study, two point-mutated
proteins each with a single base substitution [alanine for tryptophan
(W14 0A) and alanine for lysine (K13 3A) ] and ... the mutants without tryptophan at 140
(W14 0A and W140O) was much lower than both the
wild-type protein and mutants E142O and K13 3A.
Thermal analysis of protein unfolding
The DSC curves ofthe wild-type ... investigated. Two point-mutated proteins, with a single
base substitution of alanine for tryptophan (W14 0A)
and alanine for lysine (K13 3A) , and two truncated
fragment proteins, with fragments...
... Exploring theroleofa glycine cluster in cold adaptation
of an alkaline phosphatase
Konstantinos Mavromatis
1,
*, Iason Tsigos
2,
*, Maria Tzanodaskalaki
2
, Michael Kokkinidis
1,3
and Vassilis ... the aromatic ring of Tyr269, and these
unfavorable interactions could lead to a decrease of local
flexibility and an increased E
a
value.
The validity ofthe above interpretation was further
reinforced ... exhibits an E
a
almost the same as inthe case
ofthenativeenzyme(Table1).
Thermal inactivation of mutant and wild-type enzymes
In order to investigate the effects of mutations on the
stability of psychrophilic...
... ataxia telangiectasia mutated (ATM) ⁄
ataxia telangiectasia and RAD53 related (ATR)
kinases at the site of DNA damage may decrease the
kinase ⁄ phosphatase ratio and allow the phosphatase to
dephosphorylate ... lying inthe more stable interaction ofthe Pph3 phosphatase with
cH2AX as opposed to a transient interaction with Rad53p.
Abbreviations
ATM, ataxia telangiectasia mutated; ATR, ataxia telangiectasia ... the
cisplatin response [9]. Cisplatin interferes with DNA
function by causing intrastrand and interstrand cross-
linking of nucleotide bases and, in replicating cells,
DNA damage usually induces an intra-S-phase...
... intensity. As concentration of Mn increases, the intensity ofthe blue band
decreases while the intensity ofthe yellow-orange band increases gradually and reaches to the
maximum at x
Mn
= ... a blue band at 476 nm, there is one more green band at around 533 nm characterized by Cu
2+
ions (Fig. 3). Intensity ofthe green band is greater than that ofthe blue band. As increasing the ... of these two
bands are equal to each other and they merge into one wide band ranging from 476 nm to 533 nm. The
blue and green bands are characterized by radiation transition of electrons from...
... Dimensional changes in stages (material flows
processed by the logging and transport systems),
resulting from the changed performance ofthe
system, do not always cause a change in power
and material ... suitable machinery,
and in using acceptable methods for the employment
of machines with the rational performance. Pos-
sibilities to determine the rational performance of
machines are further ...
suitable rational performance. e most important
measures compensating the environment con-
tamination by extraneous substances are preventive
measures that can be applied on a larger part of the...
... Morisaki T, Matsumoto K, Tasaki A,
Yamanaka N, Nakashima H, Kuroki H, Nakamura K,
Nakamura M, Katano M. Paclitaxel probably enhances
cytotoxicity of natural killer cells against breast carcinoma ... agent. J Natl Cancer
Inst 1990, 82, 1247-1259.
22. Wiseman LR, Spencer CM. Paclitaxel. An update of its use
in the treatment of metastatic breast cancer and ovarian and
other gynaecological ... D, Mackay JA, Evans WK.
Lung Cancer Disease Site Group of Cancer Care
Ontario’s Program in Evidence-based Care. Taxanes as
first-line therapy for advanced non-small cell lung cancer: a
systemic...
... encompass an oxygen-dependent
degradation domain, responsible for hypoxic stabilization of
α-subunits, and two transactivating domains, namely the
amino-terminal transactivating domain and the carboxyl-
terminal ... hypoxia may regulate many other features that are
important in RA, such as cell trafficking and matrix degradation. An
understanding ofthe biology ofthe HIF transcription family may
eventually ... mediate
ubiquitination of various target proteins, and the intracellular
domain ofthe Notch receptor (involved inthe maintenance of
cells inan undifferentiated state), both of which notably
contain ankyrin...
... that anabolic synthesis of aggrecan aggregate
components may be affected by neutralisation of these
cytokines.
We also studied the expression ofthe aggrecanases
ADAMTS4 and ADAMTS5 in five patients. ... matrix metalloproteinase (MMP), link protein, and ADAMTS analysis, derived as described in Materials and methods and in Figures 5 and 7Polymerase chain reaction data from matrix metalloproteinase (MMP), ... aggrecanase-1 (ADAMTS4) [24] and
aggrecanase-2 (ADAMTS5) [25]. IL-1 and TNF-α can mediate
the catabolism of aggrecan, but whereas ADAMTS5 is consti-
tutively expressed, ADAMTS4 is induced after...