design conformational functional and physiological characterization of recombinant polymeric heme proteins

Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt

Ngày tải lên : 15/03/2014, 10:20

... possibility of the functional roles of the )643T>C and )1219G>A SNPs as part of the extended APOH promoter transcriptional machinery To further substantiate the functional relevance of the three ... expressed in the liver and sporadically in intestinal cell lines and tissues [8] The plasma concentration of b2GPI is 20 mgÆdL)1, of which a small portion is bound to lipoproteins and the rest exists ... altering the speciﬁcity and kinetics of the transcriptional process Given the importance of promoters in harboring functionally relevant SNPs that regulate gene expression and phenotypic variation,...

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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

Ngày tải lên : 16/03/2014, 01:20

... to Erlandsen and Stevens [22] The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, ... HPA II patients and seven HPA III patients 2050 Characterization and functional analysis of novel mutations Among the mutations identied in our HPA population, two (i.e p.Q301P and c.707-2delA) ... family and a shorter fragment of 253 bp present only in the proband, as well as in his mother who bears the same mutation (Fig 3) Direct sequencing of both cDNA bands conrmed the skipping of the...

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Báo cáo hóa học: "Reference values and physiological characterization of a specific isolated pig kidney perfusion model" doc

Ngày tải lên : 20/06/2014, 00:20

... Journal of Occupational Medicine and Toxicology 2007, 2:1 Background A variety of isolated and perfused kidney models has been used for the study of renal functional parameters ... for the performance of the isolated pig kidney Materials and methods Animals and experimental groups After approval of the local official veterinarian institutions, German landrace female pigs ... elevated values of 11.4 mg/dl (group C) and 12.9 mg/dl (group A) and significant alterations from Page of 13 (page number not for citation purposes) Journal of Occupational Medicine and Toxicology...

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Structural and binding characterization of the antiviral host proteins, VIPERIN and VAPC

Ngày tải lên : 09/09/2015, 18:57

... Expression and purification of full length VAPC and V truncated constructs 3.2.5.2 Expression and purification of NS5B 73-74 74-75 3.2.6 Preparation of Isotope Labeled Proteins 76 3.2.7 Determination of ... sequence and reaction 31-32 Figure 2.2.1 Secondary structure prediction and truncation representation 36 Figure 2.3.1 FPLC and DLS profiles of viperin 43-44 Figure 2.3.2 Far UV and Near UV CD and ... UV CD and 2D NMR spectra of unreconstituted viperin 45-46 Figure 2.3.3 CD, UV and 1D characterization of viperin (45-361) 48-49 Figure 2.3.4 CD and NMR characterization of viperin C-terminal 50-51...

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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and ﬂux control analysis ppt

Ngày tải lên : 07/03/2014, 17:20

... overexpression and purication of recombinant glycolytic enzymes The genes of HK, HPI, PPi-PFK, ALDO, TPI, GAPDH, PGK, PGAM, ENO and PPDK were cloned and the proteins overexpressed and puried (Fig ... with a higher concentration of PGAM than of PPDK at pH The control analysis showed CJ Ei values of 0.08 and 0.2 for PGAM and 0.85 and 0.57 for PPDK at 10 and 39 mU of added PPDK, respectively ... BL21DE3pLysS The preparation and characterization of recombinant EhPPDK was as described previously [11] Overexpression and purication of recombinant enzymes One-hundred millilitres of LB (LuriaBertani)...

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Báo cáo Y học: Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase pptx

Ngày tải lên : 08/03/2014, 22:20

... assay [13] Recombinant human kynureninase from the final anion-exchange step was assayed for purity using both SDS/PAGE and MALDITOF MS of the whole protein Both purified and crude samples of recombinant ... FEBS 2002 Recombinant human kynureninase (Eur J Biochem 269) 2071 tryptic mass fingerprint obtained by matrix-assisted laser desorption ionization time -of- flight (MALDI-TOF) MS of a band of the expected ... possibility of the existence of two isoforms of the enzyme as discussed by Toma et al [8] The pH optimum of 8.25 is consistent with previously reported experimentally determined [6] values, and the...

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Báo cáo khóa học: Identiﬁcation of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

Ngày tải lên : 16/03/2014, 16:20

... used as the standards and their elution volumes, Ve, were determined The standard curve was plotted with the logarithm of molecular mass against Ve/V0 of the standard protein Peptidase and ATPase ... the recombinant Bt-Lon Fig Estimation of the molecular mass of native Bt-Lon by analytical gel ﬁltration The semilogarithmic plot of elution volume (Ve/V0) vs log (molecular mass) of standard proteins ... deconvolution of this spectrum yielded 40% a-helix, 30% b-sheet, and 30% random coil and was similar to that of E coli Lon [43] The result of the unfolding transition showed a midpoint of 71.5 °C,...

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Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

Ngày tải lên : 16/03/2014, 18:20

... Biosciences) Standards consisted of lL aliquots of serial dilutions of the reaction mixtures, with the moles of phosphate deﬁned using the ATP concentration Division of the signal per mole of substrate ... higher levels of expression than the midbrain and 3550 B Sahin et al (Eur J Biochem 271) Ó FEBS 2004 Fig Deduced amino acid sequence alignment of the long and short isoforms of human and mouse AK ... more prominent TH band to be visible likely to represent the long and short isoforms of the enzyme Many of the tissues included in this analysis also showed a prevalence of one isoform over the other...

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Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identiﬁcation of residues essential for glucanosyltransferase activity and folding pot

Ngày tải lên : 16/03/2014, 18:20

... features of proteins of the GH72 family and demonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p Materials and methods Strains and growth ... determined The effects of the replacement of C74, C105 and C265 with a serine residue on the expression and complementation of the mutant phenotype of the gas1 null mutant of S cerevisiae were ... pairing of the forward primer XHup and reverse primer Ó FEBS 2004 Production and characterization of Gas1p (Eur J Biochem 271) 3637 RMGLN262 (5¢-TACAACCGTATTGAGAGAAGA AAAC-3¢), and a pairing of the...

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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

Ngày tải lên : 17/03/2014, 10:20

... proprothrombin), proFIX18 (residues )18 to )1 of proFactor IX), proFIX28 (residues )18 to +10 of proFactor IX), pro-e-TxIX/12 (residues )12 to )1 of e-TxIX precursor), e-TxIX12 (residues 1–12 of e-TxIX), ... of either the recombinant Conus carboxylase or the recombinant bovine carboxylase, and monitored carboxylation of FLEEL or e-TxIX12 as a function of propeptide concentration The effect of proFIX18 ... properties of the recombinant Conus carboxylase The recombinant carboxylase has functional properties that are similar to those of bovine carboxylase, with the exception of propeptide binding and stimulation...

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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

Ngày tải lên : 23/03/2014, 09:20

... procedures of the porcine 2-5A synthetase (data not shown) Figure demonstrates the results of the purification of the recombinant proteins The occurrence of dominant bands of the recombinant proteins ... characterized by means of a recombinant protein technique Results Expression and purification of His-tagged proteins N- and C-terminally hexahistidine tagged constructs of the 2-5A synthetase ... evidence of a high degree of purification obtained by affinity chromatography Additionally, some fainter bands of higher and lower molecular weight could be seen in the preparations (Fig 1A) Bands of...

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Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana doc

Ngày tải lên : 23/03/2014, 21:21

... plot As a crude extract of recombinant AtNIT1 had a half-time of days at pH and °C, the in¯uence of antioxidants and protease inhibitors on enzyme stability was investigated Of the applied thiols ... is in good agreement with a reported value of mM [4] Enzyme stability Fig Puri®cation and characterization of recombinant AtNIT1 in E coli (A) Lysate of isopropyl b-D-thiogalactopyranoside-induced ... SDS/PAGE and stained with Coomassie Last lane, molecular masses of standards (kDa); lanes 17±19, active fractions after HiTrap chromatography (C) Estimation of native molecular mass of recombinant...

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Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot

Ngày tải lên : 24/03/2014, 04:21

... analysis of the calculated structures revealed a short distances between NH of Leu7 and the side chain carboxyl group of the Asp6 residue (< 0.28 nm), and NH of Leu7 residue and C¼O of Leu5 (< ... FEBS 2001 Design of a potent endozepine antagonist (Eur J Biochem 268) 6049 Fig RP-HPLC monitoring of N- and C-terminal deprotections and on-resin cyclization of the linear precursor of cyclo128 ... formation of intramolecular hydrogen bonds (i, i 2) between the C ¼ O of Pro2 and the NH of Leu4 to form a ring associated with a standard g turn, and (c) a hydrogen bond (i, i 3) between the C ¼ O of...

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Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior of the peptidase pdf

Ngày tải lên : 30/03/2014, 04:20

... concentrated on kinetic characterization of LAB physiological activity As a consequence, there is little information on the expression of recombinant prolidases, the functionality of each residue or ... peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion Cloning and expression of Lc lactis prolidase The prolidase gene of Lc lactis NRRL B-1821 ... large amount of recombinant protein under the preferred growth conditions (37 °C in LB broth) of the Escherichia coli TOP 10 F’ hosts The amount of recombinant protein reached about 50% of total...

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Báo cáo khoa học: A novel plant protein disulﬁde isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx

Ngày tải lên : 30/03/2014, 04:20

... sequence of the ORF of the GmPDIM gene was identical to that of the cDNA Comparison of the soybean genomic sequence of GmPDIM with those of A thaliana (AGI number At1g04980 and At2g32920) and Oryza ... isomerase (PDI) family of proteins [2–4] In humans, 17 genes of the PDI family have been identiﬁed [5] The physiological role of each PDI protein and their interactions with each other and with other ... immunoreacted to recombinant GmPDIM by western immunoblot (Fig 2A, lane 1), and also to two bands from cotyledon cell extract of 50 and 52 kDa (Fig 2A, lane 2) The intensity of these bands decreased...

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Báo cáo sinh học: " Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes" docx

Ngày tải lên : 18/06/2014, 18:20

... amplification of plasmid T3 and the recombinant one (lanes and 4, respectively); RT-PCR on RNA of YF17D/E200T3 and YF17D/Esa/5.1glic 2P viruses without and with the EGFP cassete (lanes and 6, respectively) ... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... independent series of serial passages (at MOI of 0.02); P1 and P2 were analyzed by RT-PCR and flow citometry at passages and 10 and are represented in all panels as 5P1, 10P1, 5P2 and 10P2 In these...

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Báo cáo hóa học: " Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes" pdf

Ngày tải lên : 20/06/2014, 01:20

... amplification of plasmid T3 and the recombinant one (lanes and 4, respectively); RT-PCR on RNA of YF17D/E200T3 and YF17D/Esa/5.1glic 2P viruses without and with the EGFP cassete (lanes and 6, respectively) ... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... independent series of serial passages (at MOI of 0.02); P1 and P2 were analyzed by RT-PCR and flow citometry at passages and 10 and are represented in all panels as 5P1, 10P1, 5P2 and 10P2 In these...

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DNA by Design. Stephen Meyer and the Return of the God Hypothesis

Ngày tải lên : 01/11/2013, 07:20

... Commission of Civil Rights in August 1998; at a briefing for some members of Congress and their staffs on Scientific Evidence of Intelligent Design and Its Implications for Public Policy and Education ... ID theorists purport, and the suggestion of a conspiracy of silence is absurd One can find any number of discussions of the Cambrian radiation in the scientific literature, and new studies regularly ... what was designed and what wasn’t; and when, where, how, and by whom was design information supposedly inserted?7 Although his Discovery Institute biography describes Meyer as the author of “numerous...

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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

Ngày tải lên : 14/02/2014, 22:20

... studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of proteins I Effect of urea ... production, purication and characterization of wild-type enzyme and mutants at Gln11 Protein Eng 8, 261273 49 Burlingham BT & Widlanski TS (2001) Synthesis and reactivity of polydisulfonimides ... 29372945 50 Burlingham BT (2002) Design and synthesis of chemical probes for the investigation of enzyme recognition of anionic substrates PhD Thesis, Department of Chemistry, Indiana University,...

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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

Ngày tải lên : 18/02/2014, 06:20

... NRE(A+B)); and in both Box A and B, NRE(A–B–) (C) Qualitative analysis of LacZ reporter activation in the interaction of APUM-2 and APUM-7 with NRE WT and NRE mutants The iron responsive element RNA and ... for 22 of 25 putative PUF proteins The three remaining proteins can be divided into a closely-related pair and a single outsider (Fig 1A) The large number of copies of highly similar proteins ... has been suggested in yeast and Drosophila, APUM proteins may be involved in the regulation of many aspects of growth and development Interaction between APUM proteins and transcripts possessing...

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