... 66(1):167-167.
38 . Smith KR. The role of sperm-mediated gene transfer in genome mutation and evolution. Med Hypotheses
2002; 59(4): 433 - 437 .
39 . Zani M, Lavitrano M, French D, Lulli V. The mechanism of ... contributed to the
embryo, the resultant offspring will be chimeric (up to ca. 50% of animals). The ESC contribution to a
mouse can high (up to ca. 80% ofthe cells), and will often include the germline ... prospects for gene therapy. Gene Ther. 2002;
9(11):7 13- 718.
85. Lipps HJ, Jenke ACW, Nehlsen K, et al. Chromosome-based vectors for gene therapy. Gene Ther. 20 03;
30 4: 23- 33.
86. Abbondanzo...
... Saini J.S. and Solanki S.C. Heattransferand friction in solar air heater duct with
V-shaped rib roughness on absorber plate. Int. Journal ofHeatand Mass Transfer 2002; 45: 33 83-
33 96.
[19] ... S.J. The effect of gap position in discrete ribs on
local heat/ mass transfer in a square duct Journal of Enhanced heattransfer 20 03; 10 (3) : 287 -30 0.
[ 13] Gupta D. Solanki S.C and Saini J.S. Thermo-hydraulic ... value of turbulent intensity is observed at the relative gap width of 1.0 which is in the line ofheat
transfer measurements. These results of PIV showed good agreement between theheat transfer...
... increase)
Caspase -3 activity (fold increase)
50
30
20
10
0
3
4
2.5
3. 5
3. 5
3
2.5
2
1.5
1
0.5
0
0.5 mmol·L
–1
H
2
O
2
2
1.5
1
0.5
0
Ctrl
Ctrl
pcDNA3.1
pcDNA3.1-C 23
pcDNA3.1-Hsp70
pcDNA3.1-C 23 + pcDNA3.1-Hsp70
pcDNA3.1 ... lost.
Taken together, our results demonstrate an impor-
tant relationship among the regulation of nucleo-
lin ⁄ C 23, the activation of caspase -3 andthe induction
of apoptosis under the setting of oxidative ... minimize
the number of animals used and their suffering.
H
2
O
2
pcDNA3.1
100
10
0 0.5
Time (h)
1 23
Bcl-2 mRNA/GAPDH mRNA (%)
pcDNA3.1 pcDNA3.1-C 23
Bcl-2
GAPDH
A
B
Fig. 5. Effect of nucleolin ⁄ C23...
... new mutants of
the 3 -end of minus-strand RNA.
Results
Effect of mutations in the3 - or the 5¢-end of the
(–)IRES HCV RNA template on RNA synthesis
The secondary structure ofthe3 -terminal ... RNA synthesis
by recombinant HCV NS5B using deletion mutants of
the 3 terminus ofthe minus-strand RNA but deletions
were made on the basis ofthe structure ofthe 5¢UTR of
the plus-RNA, the structure ... further information on the replication mechanism
of the hepatitis C virus (HCV), we have studied the role of sequences at
the 3 -end of HCV minus-strand RNA in the initiation of synthesis of the
viral...
... detection of proteins crosslinked
Fig. 2. Formation of two major complexes between Swiss 3T3 proteins
and the3 -UTR ofthe MARCKS mRNA. (A) The MARCKS 3 -UTR,
the stop codon UAA ofthe coding ... (CDS) and MARCKS 3 -UTR were driven
by a CMV-promoter and used for transfection of Swiss 3T3 fibro-
blasts. Length ofthe MARCKS 3 -UTR sequences [38 ] of pDK1:
131 0–2597 bp (complete 3 -UTR of MARCKS); ... 2002)
Eur. J. Biochem. 270, 35 0 36 5 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 033 96.x
NP-40, 10 m
M
NaCl, 3 m
M
MgCl
2
and 10 m
M
Tris/HCl
pH 7.4) prior resuspending the nuclear pellet in glycerol
storage...
... C-terminal tyrosine of a-tubulin by 3-
nitrotyrosine alters the ability ofthe molecule to form
microtubules. For this purpose, we studied the behavior of
nitrotyrosinated tubulin (and of tyrosinated ... D and G vs. H;
Fig. 5B); (b) the normal appearance and proliferation of
cells subjected to cycles of growth and passage for
45 days in the continuous presence of 500 l
M
nitrotyro-
sine; (c) the ... present in the cell.
During the experiment shown in Fig. 3, number and
viability of cells were determined every day. The number of
cells increased during the first two days and then remained
constant...
... Homology model ofthe subunit of 3- ureido-
propionase, based upon the crystal structure of the
D. melanogaster 3- ureidopropionase (Protein Data
Bank code: 2VHH).
Fig. S3. To determine whether 3- ureidopropionase
deficiency ... dithiothreitol at
)80 °C. The purity ofthe recombinat protein was ‡ 85% as
judged by SDS ⁄ PAGE.
Molecular size ofthe 3- ureidopropionase
The molecular size and oligomeric state ofthe 3- ureidopro-
pionase ... fusion
protein under the control ofthe 3- ureidopropionase
promoter.
Results and Discussion
Cloning and expression of recombinant
3- ureidopropionase
The sole homolog of 3- ureidopropionase in...
... aB-crystallin (i.e. the effect of
the additive on the aggregation ofthe target protein is inde-
pendent and different from that ofthe chaperone). The
standard errors associated with this theoretical ... increased the chaperone activity of aB-crystallin
(84 ± 4%) against this target protein.
The effect of Arg-HCl on the structure and
assembly of aB-crystallin
We investigated whether the effects of these ... further enhanced by it (Fig. 1C). The sig-
nificant decrease in the amount of precipitation in
the presence of high concentrations of Arg-HCl
in the absence ofthe chaperone precluded analysis of
the...
... Terminology 131
C. Literature Review 133
1. Studies of children and adolescents 133
2. Premenopausal women 135
3. Perimenopause 137
4. Postmenopausal women 137
5. Physical activity and prevention of falls ... The Well-being of Canadians. Canadian Fitness and Lifestyle
Research Institute: Ottawa.
33 . Dyck, L. (1998). The exercise of daily life: Towards an understanding ofthe meaning and practice
of ... researchers expand the definition of health to include the social determinants of health. In a recent
article on women’s health andthe contribution of physiotherapists, McComas and Harris [38 ] use...
... about
the regulation ofthe polymer at the genetic level of its
synthesis and degradation. The gene for polymalatase
has been sequenced (accession number AJ5 433 20 [14]),
and distinct features of ... ([6] and
references therein), suggesting the presence of detect-
able amounts of PMLA synthetase-specific mRNA.
The absence of PMLA and polymalatase in other cell
types could be the result of cell ... 3a
by 14 amino acid substitutions, eight of them located
in the first two b-strands ofthe N-terminal half of
NKA48, upstream ofthe homodimer interface and
accessible for interactions with other...